A special elution buffer system for significantly removing half-antibody fragment impurities in double antibody culture
By using a special elution buffer and rinsing technique in the affinity chromatography step, the problem of removing semi-antibody fragment impurities in bispecific antibody culture was solved, achieving high-purity bispecific antibody purification that meets commercial production standards.
CN122404472APending Publication Date: 2026-07-17INTELLECTIVE BIOLOGICS (SUZHOU) CO LTD
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INTELLECTIVE BIOLOGICS (SUZHOU) CO LTD
- Filing Date
- 2026-05-28
- Publication Date
- 2026-07-17
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Figure CN122404472A_ABST
Abstract
The application discloses a special elution buffer system for significantly removing half-antibody fragment impurities in double-antibody culture and belongs to the technical field of biology. In the elution stage of the eluent in the affinity chromatography capture, the application adds ammonium sulfate to the eluent, removes the half-antibody fragment, improves the purity of the target protein after the affinity chromatography, and makes the purity of the double-antibody meet the demand of a medicine (the SEC-HPLC purity is greater than 97%) after the purification. By using the elution system method, the purity of the half-antibody fragment nr-CE is reduced from 6% to 0.2%.
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