Construction of prokaryotic expression vector of pig AANAT protein, method for preparing fusion protein and application thereof
By constructing a prokaryotic expression vector for porcine AANAT and preparing antibodies, the problem of detecting porcine AANAT protein was solved, achieving efficient and specific detection of porcine AANAT protein and meeting the needs of immunological and functional research.
Patent Information
- Application Number
- CN202610573478.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies lack effective methods for detecting porcine AANAT proteins. Artificial synthesis is costly and has poor stability, while proteins prepared by peptide methods have insufficient immunogenicity, failing to meet the needs of immunological detection and functional research.
Amplification primers were designed and synthesized to construct the porcine AANAT prokaryotic expression vector pET32a(+)-pAANAT. The His-tag-pAANAT fusion protein was expressed using the BL21(DE3) recombinant protein expression strain. Soluble protein was obtained through purification, and polyclonal and monoclonal antibodies were prepared for the detection of porcine AANAT protein.
A His-tag-pAANAT fusion protein with high expression level and high purity was obtained. The prepared antibody can specifically bind to AANAT protein in porcine pineal gland tissue, providing efficient ELISA detection conditions.
Smart Images
Figure CN122405618A_ABST