Dioscorea yunnanensis ethylene response factor gene pkerf2 and application thereof
By overexpressing the PkERF2 gene in Polygonatum yunnanense, the biosynthesis of diosgenin was regulated, which solved the problems of germplasm degradation and decline in medicinal quality in the artificial cultivation of Polygonatum yunnanense, and achieved a significant increase in diosgenin content and accumulation of medicinal components.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- KUNMING UNIV OF SCI & TECH
- Filing Date
- 2026-05-27
- Publication Date
- 2026-07-17
AI Technical Summary
Artificial cultivation of Polygonatum yunnanense suffers from problems such as germplasm degradation, inconsistent seedling quality, long planting cycle, insufficient environmental adaptability, and declining medicinal quality, which limits the development of the industry.
By cloning the ethylene response factor gene PkERF2 of Polygonatum yunnanense and constructing the pCAMBIA2300S-PkERF2 recombinant vector, PkERF2 was overexpressed in Polygonatum yunnanense through Agrobacterium tumefaciens transformation, thereby regulating the expression of genes related to diosgenin biosynthesis.
It significantly increased the content of diosgenin in Polygonatum yunnanense, promoted the accumulation of medicinal components, improved the quality of medicinal materials, and elucidated the relationship between SA signal transduction and secondary metabolism regulation.
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Figure CN122405657A_ABST