Cosmetic composition comprising lotus extract blend and method for reducing facial perspiration

Abstract

The present invention provides cosmetic compositions and methods comprising a composition derived from a lotus plant effective to treat, reduce, ameliorate and / or eliminate signs and results of dermatological aging of the skin and / or to improve the aesthetic appearance of the skin. Further, the composition is provided for use in compositions and methods to treat, ameliorate and / or reduce hyperhidrosis (or sweating or perspiration), particularly facial perspiration. More particularly, the active composition is an extract of lotus and / or a blend of extracts of various species of the lotus family, such as NELUPURE (INCI: Propanediol (and) Glycerin (and) Nymphaea Caerulea Flower Extract (and) Nelumbo Nucifera Flower Extract). This active reduces sebum and perspiration and is used to provide cosmetic long-lasting compositions.

Description

Cross-reference to related applications

[0001] This application claims the benefit of priority to U.S. Provisional Patent Application No. 63 / 614,226, filed December 22, 2023, entitled “Cosmetic Composition,” the disclosure of which is incorporated herein by reference in its entirety. Background Technology

[0002] For regular cosmetics, users often have to choose between products that are easy to remove and products that stay on the skin for a longer period of time (e.g., all day). Attached Figure Description

[0003] The accompanying drawings illustrate various aspects of this disclosure by way of example rather than limitation.

[0004] Figure 1 This is a graph showing the amount of facial sweat collected from skin treated with 2% NELUPURE facial serum in one instance.

[0005] Figure 2 This is a graph showing the amount of facial sweat collected from skin treated with 0.2% NELUPURE facial serum in one instance. Summary of the Invention

[0006] The disclosed aspects relate to a cosmetic composition comprising any of the components disclosed herein. This disclosure provides compositions and methods comprising newly discovered lotus-derived components that are effective in treating (including preventing, reducing, improving, and / or eliminating) signs and consequences of dermatological aging of the skin, particularly wrinkles and fine lines, and / or improving the aesthetic appearance of the skin. Furthermore, these components are provided for use in compositions and methods to treat, including preventing, improving, and / or reducing hyperhidrosis (or sweating or perspiration) and its associated effects, such as odor. More specifically, the active ingredient (also referred to herein as an active agent, component, ingredient, reagent, or compound) is an extract of lotus and / or a blend of extracts from various species of the lotus family, such as, but not limited to, NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily (Nymphaea Caerulea) flower extract (and) Chinese lotus (Nelumbo Nucifera) extract). These plant materials have recently been found to treat wrinkles, fine lines, and other signs of dermatological aging (e.g., internal aging) or skin exposure to sunlight (e.g., external aging), and because the plant materials act on muscle cells and nerve cells, as well as the neuromuscular junction (NMJ) of tissues and sweat gland fibers to inhibit the release of acetylcholine, for example at the NMJ, thereby relaxing or decontracting muscles such as facial expression muscles, they can be used to treat hyperhidrosis.

[0007] It has been discovered that incorporating active ingredients that reduce epidermal sebum production can yield cosmetic compositions that remain on the skin for extended periods. This disclosure provides a long-acting composition comprising an active ingredient. The active ingredient may include a material that inhibits epidermal sebum production. On one hand, the active ingredient downregulates epidermal sebum production by inhibiting leptin receptors. On the other hand, the active ingredient downregulates epidermal sebum production by competitively inhibiting cell surface leptin receptor activity. On the other hand, the active ingredient downregulates epidermal sebum production by downregulating nicotinic acetylcholine receptors (nAchR). On the other hand, the active ingredient downregulates epidermal sebum production by competitively inhibiting nAchR activity. On the other hand, the active ingredient inhibits acetylcholinesterase activity; production; and / or stability. On the other hand, the active ingredient inhibits acetylcholine release.

[0008] In some respects, the skin for which the disclosed composition is required may be skin with excessive sebum. In another aspect of the method for treating skin with excessive sebum, the skin benefits may be selected from the group consisting of: restoring a matte texture to the skin; nighttime skin type; reducing the oiliness / greasiness of the skin and / or hair; reducing dandruff production; reducing pore blockage / clogging; reducing acne production; reducing acne lesions; reducing areas of lipid overexpression or overproduction; reducing skin thickness affected by lipid overproduction; and / or combinations thereof.

[0009] Another aspect of this disclosure provides compositions, formulations, and methods containing materials newly identified for treating wrinkles, fine lines, folds, furrows, and other signs of aging skin, in addition to treating, preventing, or reducing hyperhidrosis. According to this disclosure, these materials exert their efficacy by acting on the neuromuscular junction (NMJ), such as in muscle and nerve cells or tissue layers at the application site, for example, in the skin of the face, neck, arms, feet, or hands, or in the dermis of the skin where sweat glands are located. More specifically, extracts from flowers of one or more species of lotus block or inhibit the release of acetylcholine at the NMJ of skeletal muscle, which in turn causes contracted muscles to relax or discontract. Contracted muscles are associated with the formation of fine lines, wrinkles, etc.

[0010] It should be understood that, as used herein, the terms "treating" and "treatment" include and encompass the prevention, reduction, improvement, enhancement, mitigation, and / or elimination of the dermatological effects of aging and sun exposure, particularly concerning wrinkles, fine lines, folds, grooves, and creases of the skin. This disclosure further covers the treatment of "marionette" lines extending on either side of the mouth as defined above, as well as fine lines on the forehead and vertical lines between the eyebrows. The compositions and methods of this invention are also applicable to the treatment of dermatological conditions in many areas of the body as defined above, including but not limited to the face, forehead, neck, arms, hands, legs, knees, feet, chest, back, groin, and buttocks. Detailed Implementation

[0011] Reference will now be made to certain aspects of the disclosed subject matter. While the disclosed subject matter will be described in conjunction with the enumerated claims, it should be understood that the illustrated subject matter is not intended to limit the claims to the disclosed subject matter.

[0012] Throughout this document, values ​​expressed in range format should be interpreted flexibly to include not only the numerical value explicitly stated as the limit of the range, but also all individual numerical values ​​or subranges covered within the range, as if each numerical value and subrange were explicitly stated. For example, the range “about 0.1% to about 5%” or “about 0.1% to 5%” should be understood to include not only about 0.1% to about 5%, but also the individual values ​​within the range shown (e.g., 1%, 2%, 3%, and 4%) and subranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%). Unless otherwise indicated, the statement “about X to Y” has the same meaning as “about X to about Y”. Similarly, unless otherwise indicated, the statement “about X, Y, or about Z” has the same meaning as “about X, about Y, or about Z”.

[0013] In this document, unless the context clearly indicates otherwise, the terms "a / an" or "the" are used to include one or more, or one or more kinds. Unless otherwise specified, the term "or" is used to mean a non-exclusive "or". The statement "at least one of A and B" has the same meaning as "A, B, or A and B". Furthermore, it should be understood that the wording or terms used herein, unless otherwise defined, are for descriptive purposes only and not restrictive. Any use of section headings is to aid in the reading of this document and should not be construed as restrictive; information relating to section headings may appear within or outside that particular section.

[0014] As used herein, the term "composition" includes compositions intended for application to body surfaces (e.g., skin or hair). The term "composition" may also include cleaning compositions, such as fabric care compositions or household care compositions, including air care compositions (e.g., air fresheners), for use on clothing or other substrates such as hard surfaces (e.g., tableware, floors, countertops). Further non-limiting examples of "composition" may include facial or body powders, deodorants, foundations, body / face oils, mousses, creams (e.g., cold creams), waxes, sunscreens and sunblocks, bath and shower gels, lipsticks, self-tanning compositions, face masks, and patches.

[0015] As used herein, the term “consumer” refers to the user of the composition and observers in or around the user.

[0016] It should be understood that the test methods disclosed in the Test Methods section of this application can be used to determine the corresponding values ​​of the parameters as described herein and the applicant's disclosures as claimed.

[0017] In all aspects of this disclosure, unless the context clearly indicates otherwise, all percentages are based on the weight of the total composition, as will be apparent from the context. Unless otherwise clearly stated, all ratios are by weight, and unless otherwise clearly stated, all measurements were performed at 25°C.

[0018] While easily removable products can be quickly removed, for example, by washing with soap and water, users may find themselves repeatedly reapplying the product throughout the day, as it can also be unintentionally removed by sweat and contact with clothing. On the other hand, typical long-lasting cosmetics are less likely to be accidentally removed, but can be difficult to wash off and may require the use of organic solvents or other irritating cosmetic removers. Therefore, there is a persistent need in the art for cosmetic compositions that remain on the skin for extended periods while being easily removable by washing with soap and water. Transfer-resistant cosmetics typically employ film-forming polymers to provide a long-lasting film on the skin, lips, hair, or eyelashes and facilitate the diffusion and adhesion of the formulation to the surface. Such film-forming polymers can include polymers such as organosiloxanes, including polydimethylsiloxane (PDMS or dimethicone), used due to their excellent diffusion properties and bioinertness. Recently, the properties of silicone polymers have been modified by copolymerizing them with other polymers such as polyurethanes, olefinically unsaturated monomers, or polymers thereof. For example, in U.S. Patent Publication 2008 / 0019932, the contents of which are hereby incorporated by reference, Revlon describes a cosmetic composition comprising “at least one silicone film-forming polymer, at least one pigment, and at least one dispersant that facilitates the dispersion of the pigment and the silicone film-forming polymer in the composition.”

[0019] Aesthetic conditions of the skin are often associated with natural or environmental factors, such as the aging process (e.g., intrinsic aging), exposure to sunlight or the resulting ultraviolet radiation (e.g., extrinsic aging). Other factors, such as improper care and / or diet, stress, nutritional deficiencies, repetitive facial movements, and genetic predisposition, also contribute to the development of unfavorable skin conditions, such as fine lines, frown lines, wrinkles, furrows, and creases.

[0020] Skin aging and wrinkling are multifactorial processes. The formation of fine lines and wrinkles can be attributed to both external and internal causes. External causes include exposure to ultraviolet radiation and unquenched reactive oxygen species, smog, and other pollutants. Internal factors include degradation of the skin matrix, volume loss (fat and bone), and muscle tension.

[0021] With each smile, glance, laugh, or frown, the contraction of facial muscles causes dynamic wrinkles to appear on the face. In younger skin, these fine lines fade quickly and are not prominent. However, as we age, these lines become deeper and more noticeable. Most commonly, these fine lines appear as deep grooves between the eyebrows ("frown lines"), around the eyes ("crow's feet"), and on the forehead ("forehead lines").

[0022] The biochemistry and biomechanics of skeletal muscle contraction are well characterized. Contraction is triggered by an electrical signal (action potential) transmitted by a motor neuron. At the neuromuscular junction, the action potential causes vesicles to release the neurotransmitter acetylcholine (“ACh”), which then diffuses across the nerve ending and the synapse, i.e., the muscle fiber membrane. ACh binds to ACh receptors, triggering a diffused action potential and causing the release of calcium ions.

[0023] Muscle groups exhibit varying sensitivities to muscle relaxants. Muscles in the periorbital and glabellar regions are particularly sensitive to ACh and therefore readily treatable with agents that block ACh release. See, for example, the review article by L. Vimal and O. Oladapo, “Pharmacology of Non-Depolarizing Muscle Relaxants,” Vol. 5, No. 7, *Update in Anaesthesia*, World Journal of Electronic Education in Anesthesiology and the World Federation of Anesthesiologists. http: / / www.nda.ox.ac.uk / wfsa / html / u05 / u05 — 012.htm Accessed June 20, 2007.

[0024] Botulinum toxin (also known as Botox®, Allergen, Irvine, California) is currently popular for treating wrinkles and fine lines, but was originally used to treat muscle spasms. This toxin works by specifically inhibiting neurotransmission in nerve cells, thus causing the contracted muscles to relax (e.g., A. Blitzer et al., 1993, *Arch. Otolaryngol. Head NeckSurg.*, 119:118-122; U.S. Patent No. 6,344,461 B1 to L. Breton et al.). When injected subcutaneously, botulinum toxin can also be used to treat frown lines (creases between the eyebrows). (See JD Carruthers, 1992, *Journal of Dermatologic Surgery and Oncology*, 18:17-21; U.S. Patent No. 6,344,461 B1 to L. Breton et al.). However, the full extent of adverse effects associated with long-term use of botulinum toxin and products or treatments containing this material remains not fully determined. Botulinum toxin treatment is associated with numerous side effects, including transient fatigue, dysphagia, neck weakness, hoarseness, and localized pain. Additionally, many patients who initially respond to botulinum toxin subsequently become unresponsive to treatment. (See, for example, U.S. Patent Application No. US2002 / 00812914 to Hawrot).

[0025] Novel ingredients used as active agents in compositions and formulations applied topically, preferably topically, to the skin are beneficial to both industry and consumers for various applications in skin care and therapy, as well as personal care products. NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract, used interchangeably throughout) is a blend of extracts from two lotus species: Egyptian lotus (Egyptian blue water lily) and Sacred Lotus (Chinese lotus). It has been hypothesized that NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract) regulates sebaceous glands by inhibiting leptin receptors, preventing excessive sebum production and inflammatory processes that cause skin blemishes.

[0026] Unwind Sacred Lotus, sold by Naolys, is a material composed of active cells from the Chinese lotus. Its effects are described as "regulating the skin's nervous system; the Sacred Lotus cells have a relaxing effect that helps restore the skin's natural balance." https: / / www.unifect.com / products / unwind-sacred-lotus /

[0027] The pH of the composition may be in the range of about 4 to about 8, about 5 to about 7, less than, equal to or greater than about 4, 5, 6, 7 or about 8. The ethanol concentration in any of the compositions described herein may be less than about 78 wt% ethanol, less than about 70 wt% ethanol, less than about 60 wt% ethanol, less than about 50 wt% ethanol, less than about 40 wt% ethanol, less than about 30 wt% ethanol, less than about 20 wt% ethanol, less than about 10 wt% ethanol, or ethanol-free.

[0028] In one embodiment, the active agent for reducing perspiration may be NELUPURE™. This material is a plant complex containing flower extracts of Chinese lotus and Egyptian blue water lily. NELUPURE, as described herein, comprises the following components:

[0029]

[0030] Epidermis and dermis

[0031] Human skin comprises two main septa: the superficial outer septum, the epidermis, and the deeper septum, the dermis. The outermost layer of skin, the epidermis, typically provides some protection to the body, but fine lines and wrinkles can easily appear in this part of the skin. Both the epidermis and dermis are susceptible to the harmful effects of photodamage. The natural function of the human epidermis is to provide the skin's basic protection within the body. The dermis, which provides solid and nutritional support to the epidermis, primarily consists of fibroblasts and the extracellular matrix, which is mainly composed of substances including collagen, elastin, and glycosaminoglycans (“GAGs”). Additionally, the dermis contains leukocytes, mast cells, tissue macrophages, blood vessels, muscle cells, and nerve fibers. The dermal muscles of the face are controlled by the motor nerves of the facial nerve. The interlobular septa of the hypodermis contain fibers that make up striated muscle tissue, such as carrageenan. A subset of dermal fibroblasts known as myofibroblasts possesses contractile properties similar to those of muscle cells and tissues.

[0032] fine lines on the face

[0033] Fine lines on the face between the eyebrows are produced by the activity of the corrugator supercilii and orbicularis oculi muscles. These muscles move the eyebrows inward, and the depressor supercilii and depressor supercilii muscles pull the eyebrows downward. This produces a frown, or "furrowed brow." The location, size, and use of these muscles vary significantly between individuals. Fine lines caused by facial expressions occur perpendicular to the direction of action of the contracting facial muscles. The effective dosage for facial fine lines is determined by gross observation of the patient's ability to activate the injected superficial muscles.

[0034] Fine lines at the outer corner of the eye primarily originate from the activity of the orbicularis oculi muscle, which is responsible for blinking and eyelid closure around the eye. Strong contractions of the orbicularis oculi muscle cause folds (crow's feet) that oriented laterally and radially from the outer canthus. The distribution of these radial lines varies among patients. Forehead fine lines are mainly caused by the activity of the frontalis muscle. This muscle moves the eyebrows upward, thus interacting with the depressor supercilii, orbicularis oculi, corrugator supercilii, and depressor supercilii muscles. Contraction of the frontalis muscle causes the eyebrows to lift. The location, size, and use of this muscle vary significantly between individuals.

[0035] High seborrheic dermatitis

[0036] Some people exhibit hyperspermia, characterized by excessive sebum production and excretion. Typically, individuals with hyperspermia show sebum levels greater than 200 µg / cm² on the forehead. 2 Excessive sebum production can lead to aesthetic problems such as oily / sparkling skin or hair, acne-prone skin, enlarged pores, thickened skin, or poor makeup retention. Additionally, excess sebum can act as a catalyst for acne by clogging pores, forming comedones (“blackheads” or “whiteheads”), which, if left untreated, can become inflamed and progress to acne vulgaris. Furthermore, an odor may be emitted due to the buildup, production, or excretion of excess sebum. The compositions described herein can provide skin benefits because they prevent these undesirable effects caused by excessive sebum production.

[0037] In other aspects, skin benefits can be selected from the following groups: reduced lipid production by sebaceous glands; reduced lipid synthesis in subcutaneous adipose tissue; reduced triglyceride synthesis; reduced fatty acid synthesis; prevention and / or improvement of skin-related conditions associated with inhibition of lipolysis; prevention and / or improvement of skin-related conditions associated with non-selective or partially selective PPAR-γ stimulators / upregulators; prevention and / or improvement of skin-related conditions associated with non-selective or partially selective upregulation of adipogenesis genes (usually upregulated by PPAR-γ activation); prevention and / or improvement of skin-related conditions associated with non-selective or partially selective FAS stimulation. Skin conditions associated with stimulants / upregulators; prevention and / or improvement of skin conditions associated with non-selective or partially selective SCD-1 receptor stimulants / upregulators; prevention, improvement, or treatment of oily skin; prevention, improvement, or treatment of oily hair; prevention, improvement, or treatment of oily scalp; prevention, improvement, or treatment of enlarged pores; prevention, improvement, or treatment of acne-prone skin; prevention, improvement, or treatment of body odor associated with excessive sebum production; prevention, improvement, or treatment of adult cellulite; prevention, improvement, or treatment of excessive accumulation or production of subcutaneous fat in adults; improvement of skin texture associated with cellulite; and / or combinations thereof.

[0038] In some respects, the skin for which the disclosed composition is required may be skin with excessive sebum. In another aspect of the method for treating skin with excessive sebum, the skin benefits may be selected from the group consisting of: restoring a matte texture to the skin; nighttime skin type; reducing oiliness / greasiness of the skin and / or hair; reducing dandruff production; reducing pore blockage / clogging; reducing acne production; reducing acne lesions; reducing areas of lipid overexpression or overproduction; reducing skin thickness affected by lipid overproduction; and / or combinations thereof.

[0039] According to various aspects of this disclosure, cosmetic compositions may include active ingredients.

[0040] Acetylcholine and acetylcholine release inhibitors

[0041] The neurotransmitter acetylcholine is secreted by neurons in many regions of the brain, but specifically by the large pyramidal cells of the motor cortex, several different neurons in the basal ganglia, motor neurons innervating skeletal muscles, preganglionic neurons of the autonomic nervous system (both sympathetic and parasympathetic), sacral fibers of muscle spindle fibers, postganglionic neurons of the parasympathetic nervous system, and some postganglionic neurons of the sympathetic nervous system. Essentially, only the postganglionic sympathetic fibers leading to sweat glands, arrector pili muscles, and a few blood vessels are cholinergic, as most postganglionic neurons of the sympathetic nervous system secrete the neurotransmitter norepinephrine. In most cases, acetylcholine has an excitatory effect. However, acetylcholine is known to have an inhibitory effect on some peripheral parasympathetic nerve endings, such as inhibiting heart rate via the vagus nerve.

[0042] Efferent signals from the autonomic nervous system are transmitted to the body via the sympathetic or parasympathetic nervous system. Preganglionic neurons of the sympathetic nervous system extend from the cell bodies of preganglionic sympathetic neurons located in the middle lateral horn of the spinal cord. Preganglionic sympathetic nerve fibers extending from the cell bodies synapse with postganglionic neurons located in the paravertebral sympathetic ganglia or prevertebral ganglia. Since the preganglionic neurons of both the sympathetic and parasympathetic nervous systems are cholinergic, the application of acetylcholine to the ganglia will stimulate both sympathetic and parasympathetic postganglionic neurons.

[0043] Acetylcholine activates two types of receptors: muscarinic receptors and nicotinic receptors. Muscarinic receptors are present in all effector cells stimulated by postganglionic neurons of the parasympathetic nervous system, as well as in effector cells stimulated by postganglionic cholinergic neurons of the sympathetic nervous system. Nicotinic receptors are present in the adrenal medulla and in autonomic ganglia, specifically on the cell surface of postganglionic neurons at the synapses between preganglionic and postganglionic neurons of both the sympathetic and parasympathetic nervous systems. Nicotinic receptors are also present in many involuntary nerve endings, such as in the membrane of skeletal muscle fibers at the neuromuscular junction.

[0044] When small, transparent intracellular vesicles fuse with the presynaptic neuron cell membrane, acetylcholine is released from the cholinergic neuron. Various non-neuronal secretory cells, such as adrenal medulla (and the PC12 cell line) and pancreatic islet cells, release catecholamines and parathyroid hormone, respectively, from large, dense-core vesicles. The PC12 cell line is a widely used clone of rat pheochromocytoma cells in tissue culture models for studying sympathetic adrenal gland development.

[0045] In skeletal muscle, a neuromuscular junction is formed through the proximity of the axon to the muscle cell. Signals transmitted through the nervous system generate action potentials at the terminal axons, where ion channels are activated and the neurotransmitter acetylcholine is released from synaptic vesicles within the neuron, such as at the motor endplate of the neuromuscular junction. Acetylcholine crosses the extracellular space to bind to acetylcholine receptor proteins on the surface of the muscle endplate. Once sufficient binding occurs, the muscle cell's action potential causes a change in specific membrane ion channels, resulting in muscle cell contraction. Acetylcholine is then released from the muscle cell and metabolized by cholinesterases in the extracellular space. The metabolites are recycled back to the terminal axons for reprocessing into additional acetylcholine.

[0046] Reduced sebum and sweat production and the proposed mechanism of action (downregulation of leptin receptors / inhibition of acetylcholine release).

[0047] It has been found that incorporating active ingredients that reduce sebum production can yield cosmetic compositions that remain on the skin for extended periods. This disclosure provides a cosmetic composition comprising an active ingredient. The active ingredient may include a material that inhibits sebum production. On one hand, it has been proposed that the active ingredient downregulates sebum production through leptin receptor downregulation. On the other hand, the active ingredient downregulates sebum production by competitively inhibiting the activity of cell surface leptin receptors. Additionally, the active ingredient inhibits acetylcholine release. The active substance does not downregulate sebum production through ACh receptors. It has been found that the active substance inhibits acetylcholine release.

[0048] Mechanism of action

[0049] On one hand, the active ingredient is a leptin receptor downregulator and / or an acetylcholine release inhibitor. On the other hand, the leptin receptor downregulator and / or acetylcholine release inhibitor is an extract solution that may include extracts of Egyptian blue water lily and Chinese lotus. On the other hand, said solution is available as a product under the trade name NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract). Without intending to be bound by any theory, it is believed that said extract solution can be used in cosmetic compositions, in part because said cosmetic compositions reduce the expression of leptin receptors in sebaceous gland cells, thereby preventing the inflammatory processes that trigger sebum production and cause skin spots; and / or inhibiting acetylcholine release at the neuromuscular junction. Leptin, a protein synthesized and secreted by different types of cells in the body, including skin cells, has been shown to play a role in the cellular mechanisms that trigger sebum synthesis. The benefits of including the extract solution are at least partly unexpected because the extract solution can be an inhibitor of acetylcholine release. According to some aspects, the ability of the extract solution to serve as a component of a cosmetic composition is determined by assays used to determine sebum levels in a sample that has been in contact with the extract or a composition comprising the extract; leptin cell surface receptors in sebaceous gland cells; and / or inhibition of acetylcholine release.

[0050] The ability of extract solutions of Egyptian blue water lily extract and Chinese lotus extract to provide beneficial properties as acetylcholine release inhibitors suggests that other natural extracts with acetylcholine release inhibitory properties can also be used in the manner described herein.

[0051] According to some aspects, the ability of an extract solution to serve as a component of a cosmetic composition is determined by a assay (e.g., ELISA assay) used to determine the level of acetylcholine in a sample that has been in contact with the extract or a composition comprising the extract.

[0052] This disclosure provides novel compositions and methods comprising newly discovered components that can effectively treat and / or improve the aesthetic appearance of the skin, said treatment including preventing, reducing, improving, inhibiting, alleviating, and / or eliminating signs and consequences of dermatological aging of the skin due to time and / or hormonal aging or due to light exposure of the skin (e.g., sunlight or ultraviolet radiation), particularly wrinkles, folds, grooves, creases, and fine lines. Further, these components are provided for use in the compositions and methods to treat, including preventing, improving, inhibiting, and / or reducing hyperhidrosis (sweating or sweating) and its associated effects, such as odor and non-aesthetic appearance.

[0053] More specifically, the components of this disclosure (also referred to herein as active agents, compositions, ingredients, reagents, substances, or compounds) are derived from flower extracts of one or more species of lotus, obtained from plants, preferably from the lotus family. These plant materials have been newly identified as effective components in compositions and methods for treating, preventing, reducing, inhibiting, improving, and / or eliminating wrinkles, fine lines, folds, wrinkles, furrows, and other signs of dermatological aging due to time and / or hormonal aging or due to sun exposure of the skin, as well as for treating hyperhidrosis.

[0054] According to this disclosure, but not wishing to be bound by theory, flower extracts of one or more species of lotus exert their effects by their ability to inhibit, block, reduce, or prevent the release of acetylcholine at the neuromuscular junction (NMJ) of skeletal muscle cells and tissues, thereby relaxing muscles, such as those associated with facial movement or expression. Both nerve cells and muscle cells (skeletal muscle cells) are electrically excitable, and the junction between these types of cells is called the NMJ. It has been known for many years that stimulation of motor nerves innervating skeletal muscles also induces the release of acetylcholine, and acetylcholine, in turn, stimulates skeletal muscle contraction. Therefore, acetylcholine has been identified as a neurotransmitter at the neuromuscular junction. (See, for example, B. Alberts et al., 1989, *Molecular Biology of the Cell*, 2nd ed., Chapter 19, The Nervous System, Garland Publishing, Inc., NY., p. 1075, etc.). This disclosure provides newly described methods for inhibiting, reducing, blocking, or preventing the release of acetylcholine at the NMJ in skeletal muscle, and thus relaxing muscles, preferably those associated with facial expressions or movement, particularly those composed of one or more species of lotus flowers when used in the compositions and methods of the present invention. By inhibiting acetylcholine release, compositions and methods comprising flower extracts from one or more species of lotus flowers as described herein can reduce, treat, and / or prevent excessive sweating or perspiration.

[0055] Flower extracts of one or more species of lotus regulate motor contraction by inhibiting the release of acetylcholine from muscle cells, thereby relaxing muscle fibers in the skin or subcutaneous muscles and / or nerve tissue, thus reducing wrinkles, fine lines, folds, and grooves. By relaxing or preventing the contraction or excessive contraction of skin or subcutaneous muscle cells in areas such as the face, hands, and feet, flower extracts of one or more species of lotus and compositions containing flower extracts of one or more species of lotus can effectively smooth the skin appearance in these areas, where muscle contraction leads to the formation of wrinkles, etc. Therefore, if muscle cell contraction is associated with the formation of wrinkles, fine lines, etc., the relaxation or decontraction of contraction of skin or subcutaneous muscle tissue by flower extracts of one or more species of lotus active ingredients in the compositions disclosed herein can loosen or relax contracted muscle tissue and alleviate, reduce, improve, inhibit, or eradicate wrinkles, fine lines, etc. Contraction of skin or subcutaneous muscle cells or skin tissue can cause wrinkles, fine lines, etc., which constitute at least some visible dermatological effects of aging due to time and / or hormonal aging and / or due to light exposure, as described herein.

[0056] Therefore, in one aspect, this disclosure covers compositions, formulations, and methods that, in addition to treating, preventing, or reducing hyperhidrosis, contain newly identified components that can be used to treat wrinkles, fine lines, folds, furrows, and other signs of aging and / or skin light exposure, preferably flower extracts of one or more species of lotus. Skin in various areas of the body is suitable for treating and / or receiving compositions of this disclosure, including the face, forehead, neck, arms, legs, hands, feet, torso (chest), back, etc.

[0057] According to this disclosure, plant-derived materials from the lotus family exert their efficacy by preferably acting at the neuromuscular junction (NMJ), such as within and around muscle and nerve cells and tissues at the application site, for example, the skin of the face, neck, arms, feet, hands, or chest, or in the dermis of skin containing sweat glands. Previously unknown or recognized combinations of materials can inhibit the release of acetylcholine at the NMJ of skeletal muscle, thereby relaxing muscles associated with aesthetically unpleasant wrinkles, frown lines, fine lines, folds, grooves, wrinkles, necklines, etc. (possibly due to aging over time and / or hormones, prolonged or excessive sun exposure, UV radiation associated with light exposure, or aging caused by overuse of facial muscles).

[0058] Generally, for the purposes of this disclosure, when a substance, such as an extract of one or more species of lotus in the described composition, induces relaxation in contracted muscle cells or tissues such as skin or subcutaneous muscle tissue, the substance is considered a muscle relaxant and / or exhibits an inhibitory effect on the release of acetylcholine at the NMJ. Contracted muscle cells or tissues are associated with the formation of wrinkles, fine lines, etc. Relaxation or decongestion of contracted muscles, such as by inhibiting or blocking the release of acetylcholine at the NMJ through the action of extracts of one or more species of lotus, can smooth the appearance of the skin or provide minor relief, thereby preventing, improving, reducing, and / or eradicating the formation of wrinkles and fine lines caused by contracted muscle tissue of the skin.

[0059] For use in the compositions of this disclosure, one or more species of lotus flower extracts can be chemically synthesized. Alternatively, these plant alkaloids can be extracted from plants. When extracted, the plant alkaloids can be in pure form, semi-purified form, or can be components of unpurified plant extracts.

[0060] Flower extracts of one or more species of lotus plant material contained in the compositions of this disclosure can be chemically synthesized on a large scale, industrially. Alternatively, the material can be extracted from the natural raw material of the plant. Any and all preparation methods can be used, including the use of cultured plant seed cells, as disclosed in commonly owned U.S. Patent Application Serial No. 10 / 040,242, which is hereby incorporated by reference. For example, unpurified plant extracts can be used in this disclosure with regard to the solubility of a particular plant extract in water or organic solvents, and the extraction process can be different. Two methods for extracting material from raw plant material include organic solvent extraction and aqueous-organic solvent extraction, as described in U.S. Patent No. 6,372,239 to Wu et al. Organic extraction methods involve the steps of continuously washing the plant material and extracting the plant material against an organic solvent stream. Examples of organic solvents include, but are not limited to, methanol, ethanol, dichloromethane, chloroform, xylene, and petroleum ether. Alternatively, alkaloids can be partially or completely purified. The chemical synthesis of alkaloids avoids the need for extraction and purification.

[0061] According to this disclosure, flower extracts of one or more species of lotus comprise compositions including, but not limited to, topical formulations, antioxidants, anti-inflammatory agents, sunscreens, cosmetics, including cosmeceuticals, anti-aging formulations such as creams for fine lines and / or wrinkles, topical medications, skin penetration enhancers, antiperspirants, deodorants, etc. Also according to this disclosure, flowers of one or more species of lotus, and other ingredients comprising such compositions, can be formulated in various product forms. Preferably, the compositions are prepared in a targeted delivery system, such as transdermal, like patches, particularly for topical application.

[0062] This disclosure covers compositions comprising flower extracts of one or more species of lotus, preferably in a pharmaceutically acceptable cosmetic or dermatological formulation suitable for contact with living tissues, including human tissues, and suitable for topical application with minimal adverse physiological effects on the user, such as irritation. Therefore, the compositions of the present invention are particularly suitable for sensitive skin.

[0063] The compositions covered by this disclosure may be provided in any cosmetic and / or dermatologically suitable form, preferably as a lotion, gel, cream, or incorporation into a transdermal patch, and also as an ointment or oil-based, as well as a sprayable liquid. Other suitable cosmetic forms for the compositions of this disclosure include lotions, mousses, lip balms, lip glosses, lotions, masks, ointments, hair creams, solutions, serums, sprays, or tissues. Additionally, the compositions contemplated by this invention may include more cosmetically acceptable adjuvants commonly used and known by skilled practitioners, such as colorants, fragrances, emollients, humectants, preservatives, vitamins, chelating agents, thickeners, etc., and other botanical preparations such as aloe vera, chamomile, etc., as further described below. The compositions are preferably applied topically once or twice daily. Daily application may continue for up to two weeks, four weeks, or longer.

[0064] In one aspect of sunscreen formulations, compositions comprising flower extracts of one or more species of lotus may further include, but are not limited to, ingredients such as avobenzone, cinnamic acid derivatives (e.g., octyl methoxycinnamate), octyl salicylate, oxybenzone, titanium dioxide, zinc oxide, and mixtures or combinations thereof. Such formulations may also preferably include α-hydroxy acids, oxacids, oxadiic acids, and mixtures or combinations thereof.

[0065] The compositions disclosed herein can also be formulated into liposomes, which may contain other additives or substances and / or may be modified to more specifically reach or remain at a site after application. Alternatively, compositions containing flower extracts of one or more species of lotus may be injected subcutaneously (sc) or intradermally (id) at sites where reduction, improvement, prevention, and / or elimination of wrinkles, fine lines, etc. is desired (e.g., via microneedling, optionally in combination with a transdermal patch), due to inhibition of acetylcholine release at the NMJ and the associated relaxation of contracting muscles.

[0066] Suitable pharmaceutically or physiologically acceptable compositions for use in this disclosure include compositions in which the active ingredient, component, or material is contained in an amount effective in achieving the intended purpose. For example, in the compositions of the present invention, flower extracts of one or more species of lotus are present in an amount from about 0.0001 wt% to about 10 wt% based on the total weight of the composition. More preferably, the compositions of the present invention comprise one or more flower extracts of one or more species of lotus in an amount from about 0.0005 wt% to about 5 wt%. Most preferably, the compositions of the present invention comprise one or more flower extracts of one or more species of lotus in an amount from about 0.001 wt% to about 2 wt% of the total composition.

[0067] The determination of an effective dose or amount is entirely within the competence of those skilled in the art. A therapeutically effective dose refers to the amount of the active ingredient, such as flower extracts from one or more species of lotus identified according to this disclosure, which, for example, treats, prevents, improves, reduces, or eliminates symptoms, more specifically, wrinkles, fine lines, folds, etc. A practitioner who will consider factors relevant to the individual requiring treatment will determine the exact dose. The dose and administration are adjusted to provide an adequate level of the active ingredient or to maintain the desired effect. Factors typically considered include the severity of the individual's specific need, the patient's general health condition, the individual's age, weight and sex, diet, time and frequency of administration, drug combination, sensitivity to response, and tolerance / response to treatment. As a general guideline, durable pharmaceutical compositions may be administered daily, every 2 to 4 days, weekly, or every two weeks, depending on the half-life and clearance rate of the particular formulation. Variations in these dose levels can be adjusted using standard empirical methods well known in the art for optimization. Guidance on specific dosages and delivery methods is provided in the literature and is generally available to those skilled in the art. Those skilled in the art will use different formulations based on the properties, such as structure and composition, of the active extracts from one or more species of lotus flowers.

[0068] The compositions disclosed herein improve the aesthetic appearance of the skin by treating at least one of the following: dermatological aging, particularly signs of aging due to time, photochemical, or hormonal changes, or signs of aging due to external factors such as sun exposure. Specifically, improving the aesthetic appearance of the skin includes at least one of the following: making facial fine lines less noticeable; making facial fine lines and / or wrinkles appear filled in; improving the appearance of infraorbital and / or periorbital fine lines; improving the appearance of crow's feet; reducing and / or diminishing the appearance of wrinkles, especially wrinkles (e.g., marionette lines) on the cheeks, forehead (e.g., vertical wrinkles between the eyes, horizontal wrinkles above the eyes) and / or around the mouth, and especially deep wrinkles, folds, or creases; improving skin suppleness; reducing and / or eliminating fine lines and / or deep fine lines, folds, and creases; and smoothing the skin, for example, to the extent of reduced wrinkling / fine lines.

[0069] This disclosure covers transdermal delivery modalities, such as patches, with or without suitable skin penetration enhancers. The methods and compositions embodied in this disclosure include means for the effective administration of one or more flower extracts of one or more species, including lotus, through a transdermal system. Typically, transdermal delivery is required for compounds that are poorly absorbed locally or at high dose levels. Therefore, transdermal means of delivering compositions or formulations (typically containing skin penetration enhancer compositions) to the skin are transdermal patches or similar devices known and described in the art. Examples of such devices are disclosed in U.S. Patents 5,146,846, 5,223,262, 4,820,724, 4,379,454, and 4,956,171; such descriptions are not intended to be limiting. Transdermal modalities that store and deliver compositions onto the skin to form active compositions are convenient and well-suited to the purposes of one aspect of this disclosure, using topical patches applied to the desired area for extended periods. Preferably, the long period of time is greater than one hour; more preferably, the long period of time is overnight, for example, when the user is sleeping.

[0070] A specific aspect of this disclosure relates to the delivery of the described compositions using targeted delivery systems, such as liposomes, microspheres (see, for example, U.S. Patent No. 5,770,222 to Unger et al.), transdermal patches, etc., such that flower extracts of one or more species of lotus active ingredients can more easily reach and influence the muscle layer of the application area, such as the face or neck, or the dermis of the skin where sweat glands are located, such as the armpits, feet, hands, etc. Compositions comprising flower extracts of one or more species of lotus (including liposome formulations) can also be administered by direct subcutaneous or intradermal injection to more precisely deposit the active agent at sites where inhibition of muscle relaxation or decongestion via acetylcholine release is desired.

[0071] Liposomes and delivery systems and media involving liposomes are well known in the art. In short, liposomes are monolayered or multilayered lipid vesicles that capture a considerable portion of an aqueous solution. Liposome vesicle microreservoirs can contain a variety of water-soluble materials suspended in an emulsion (e.g., reviewed in G. Gregorius (ed.), 1991, Liposome Technology, Volumes I, II, III, CRC Press, Boca Raton, Fla.; Davis SS and Walker IM, 1987, Methods in Enzymology, 149:51-64; Mayhew E. et al., 1987, Methods in Enzymology, 149:64-77; and Shafer-Korting M. et al., 1989, Journal of the American Academy of Dermatology, 21:1271-1275). The preparation of liposomes and their various uses in biological systems have been described (e.g., in U.S. Patent No. 4,708,861 to MC Popescu et al., U.S. Patent No. 4,224,179 to M. Schneider et al., and U.S. Patent No. 4,235,871 to DP Papahadjopoulos et al.). Therefore, such liposomes can be formulated into any dermatological or cosmetic composition as described herein.

[0072] In addition to flower extracts of one or more species of lotus as active agents, as described herein, physiologically acceptable and pharmaceutical compositions may also contain suitable pharmaceutically acceptable carriers, diluents, or excipients comprising adjuvants that facilitate the formulation of the active compound into a pharmaceutically usable formulation. Further details regarding techniques for formulation and administration are provided in the latest edition of Remington's Pharmaceutical Sciences (Mack Publishing Co.; Easton, PA). Pharmaceutical compositions containing flower extracts of one or more species of lotus as disclosed herein can be manufactured in ways known in the art, for example, by means of conventional mixing, dissolving, granulating, pelleting, grinding, emulsifying, encapsulating, embedding, or lyophilizing processes.

[0073] If applicable, the pharmaceutical composition may be provided as a salt and may form with many acids, including but not limited to hydrochloric acid, sulfuric acid, acetic acid, lactic acid, tartaric acid, malic acid, succinic acid, etc. The salt tends to be more soluble in aqueous solvents or other protic solvents than its corresponding free base form. In other cases, the formulation may be a lyophilized powder.

[0074] Preferred aspects of the topical compositions disclosed herein also include at least one of the following: surface smoothing agents, skin fillers, optical diffusing agents, sunscreens, exfoliating agents, or antioxidants.

[0075] Surface smoothing agents provide functional benefits by enhancing skin smoothness and reducing the appearance of fine lines and wrinkles. Examples include, but are not limited to, isopropyl myristate, petrolatum, isopropyl lanolin, silicones (e.g., methyl methacrylate, dimethicone), or any mixture thereof. The surface smoothing agent preferably constitutes about 0.1 wt% to about 50 wt% of the total weight of the composition.

[0076] Skin tampon agents act as collagen enhancers for the skin. Suitable and preferred examples of skin tampon agents are palmitoyl oligopeptides. Other non-limiting examples of skin tampon agents include collagen and / or glycosaminoglycan (GAG) enhancers. The skin tampon agent preferably constitutes about 0.1 wt% to about 20 wt% of the total weight of the composition.

[0077] Optical diffusing agents are particles that alter the surface luster of the skin, causing visual blurring and softening of, for example, fine lines and wrinkles. Examples of optical diffusing agents that can be used in this disclosure include, but are not limited to, boron nitride, mica, nylon, polymethyl methacrylate (PMMA), polyurethane powder, sericite, silica, silicone powder, talc, Teflon, titanium dioxide, zinc oxide, or any mixture thereof. The optical diffusing agent preferably constitutes about 0.01 wt% to about 20 wt% of the total weight of the composition.

[0078] On the other hand, this disclosure includes sunscreens that protect the skin from damaging ultraviolet radiation. Illustratively, sunscreens provide UVA and UVB protection by using a single sunscreen or a combination of sunscreens. Among the sunscreens that can be used in the compositions of this invention are avobenzone, cinnamic acid derivatives (such as octyl methoxycinnamate), octyl salicylate, oxybenzone, titanium dioxide, zinc oxide, or any mixture or combination thereof. Preferably, the sunscreen is present in about 1 wt% to about 30 wt% of the total weight of the composition. In particular, it is preferable to add sunscreen to prevent / reduce photodegradation of the composition and / or its components on the skin during packaging and / or after application.

[0079] It should be understood that the compositions of this disclosure containing sunscreen provide additional improvements to the aesthetic appearance of the skin, including at least one of the following: minimizing sunburn, minimizing tanning, reducing redness, and reducing the development of future wrinkles. It should be understood that adding sunscreen may not be preferred when the topical composition is intended to be applied before resting at night.

[0080] The compositions of the present invention may also contain one or more exfoliating agents. Suitable examples of exfoliating agents that may be used in the compositions of the present invention include stratum corneum exfoliants, such as active agents having peeling, exfoliating, or scrubbing properties, or active agents that can soften the epidermal stratum corneum of the skin; α (alpha) and / or β (beta) hydroxy acids; benzoyl peroxide; keto acids, such as pyruvate, 2-oxopropionic acid, 2-oxobutyric acid, and 2-oxovalerate; oxacids, such as those disclosed in U.S. Patent Nos. 5,847,003 and 5,834,513, the disclosures of which are incorporated herein by reference; urea; retinoids or mixtures thereof. These anti-wrinkle or anti-fine line active agents may be formulated, for example, in amounts from about 0.0001% by weight to 5% by weight relative to the total weight of the composition.

[0081] More specifically, examples of hydroxy acids include, but are not limited to, straight-chain, branched, cyclic, saturated, or unsaturated α-hydroxy acids or β-hydroxy acids. The hydrogen atoms in the carbon-based backbone of these materials may be substituted with halogens, halogen-containing alkyl groups, acyl groups, acyloxy groups, alkoxycarbonyl groups, or alkoxy groups having 2 to 18 carbon atoms. Suitable hydroxy acids include, for example, glycolic acid, lactic acid, malic acid, tartaric acid, citric acid, 2-hydroxyalkanoic acid, mandelic acid, salicylic acid, and their alkyl derivatives, including 5-n-octanoylsalicylic acid, 5-n-dodecanoylsalicylic acid, 5-n-decanoylsalicylic acid, 5-n-octylsalicylic acid, 5-n-heptoxysalicylic acid, 4-n-heptoxysalicylic acid, and 2-hydroxy-3-methylbenzoic acid or their alkoxy derivatives, such as 2-hydroxy-3-methoxybenzoic acid. Exemplary retinols include, but are not limited to, retinoic acid (e.g., all-trans or 13-cis) and its derivatives, retinol (vitamin A) and its esters, such as retinyl palmitate, retinyl acetate, and retinyl propionate and their salts. Preferred exfoliating agents are 3,6,9-trioxaundecanedioic acid, glycolic acid, lactic acid, or any mixture thereof. Other acids, such as oxacids (e.g., U.S. Patent No. 6,069,169) and oxadic acids (e.g., U.S. Patent No. 5,932,229), may be included in the compositions disclosed herein.

[0082] When this disclosure includes an exfoliating accelerator, the composition typically comprises about 0.5 wt% to 30 wt%, preferably about 1 wt% to about 15 wt%, more preferably about 2 wt% to about 10 wt%, and most preferably about 4 wt% of the exfoliating accelerator, based on the total weight of the composition.

[0083] Antioxidants, in particular, play a role in scavenging free radicals from the skin to protect it from environmental damage. Examples of antioxidants that can be used in the compositions of this invention include compounds with phenolic hydroxyl functions, such as ascorbic acid and its derivatives / esters; β-carotene; catechins; curcumin; ferulic acid derivatives (e.g., ethyl ferulic acid or sodium ferulic acid); gallic acid derivatives (e.g., propyl gallate); lycopene; reducing acids; rosmarinic acid; tannic acid; tetrahydrocurcumin; tocopherol and its derivatives; uric acid; or any mixture thereof. Other suitable antioxidants are antioxidants with one or more thiol (-SH) functions, in reducing or non-reducing forms, such as glutathione, lipoic acid, thioglycolic acid, and other thiol compounds. Antioxidants can be inorganic, such as bisulfites, metabisulfites, sulfites, or other sulfur-containing inorganic salts and acids. The compositions disclosed herein may include antioxidants, which preferably comprise about 0.001 wt% to about 10 wt% of the total weight of the composition, and more preferably about 0.001 wt% to about 5 wt%.

[0084] The compositions disclosed herein may also include one or more of the following ingredients, additives, or adjuvants: anesthetics, anti-allergic agents, antifungal agents, antibacterial agents, chelating agents, colorants, dyes, modifiers, emollients, emulsifiers, fragrances, fillers, humectants, lubricants, moisturizers, pH adjusters, pigment modifiers, preservatives, skin penetration enhancers, stabilizers, surfactants, thickeners, viscosity modifiers, hydrophilic or lipophilic gelling agents, vitamins, or any mixtures thereof. The amounts of these various ingredients, additives, adjuvants, or active agents are amounts conventionally used in the cosmetic or pharmaceutical fields, for example, they may constitute from about 0.01% to 20% of the total weight of the composition. Additionally, adjuvants, ingredients, additives, or active agents may be introduced into the fatty phase, liquid phase, and / or lipid vesicles, depending on their properties.

[0085] The components of this disclosure are preferably contained in a cosmetically acceptable medium (e.g., a mordant, diluent, or carrier). In the context of topical application, the compositions of this disclosure are in a skin-compatible medium. The compositions can be formulated as aqueous, alcoholic, or aqueous / alcohol-based solutions, ointments, lotions, gels, water-in-oil, oil-in-water, or water-oil-water triple emulsions, having the appearance of a cream or gel, microemulsion, or aerosol. Additionally, as described above, the compositions can be in the form of vesicular dispersions containing ionic and / or nonionic lipids. Dosage units suitable for such pharmaceutical compositions are formulated according to conventional knowledge and techniques used in the art.

[0086] More specifically, the composition for topical application may be in the form of a protective care composition for the skin, preferably for use on the face, neck, hands, feet, or other areas of the body. Non-limiting examples include day creams or lotions, night creams or lotions, sunscreens, lotions or oils, body lotions, makeup (foundation), artificial tanning compositions, and depilatory agents.

[0087] If the composition of this disclosure is an emulsion, the proportion of fatty substances contained therein, relative to the total weight of the composition, can range from about 5% by weight to 80% by weight, preferably from about 5% by weight to 50% by weight. The fatty substances and emulsifiers used in the emulsion-form composition are one or more of those conventionally used in the cosmetic or pharmaceutical fields. Non-limiting examples of fatty substances include mineral oils (e.g., petroleum jelly), vegetable oils and their hydrogenated derivatives, animal oils, synthetic oils (e.g., perhydrosqualene), silicone oils (e.g., polydimethylsiloxane), and fluorinated oils. Other exemplary fatty substances include fatty alcohols (e.g., stearyl alcohol or cetyl alcohol), fatty acids (e.g., stearic acid), and waxes.

[0088] The emulsifier is typically present in the compositions of this disclosure in an amount of about 0.3% to 30% by weight, and preferably about 0.5% to 30% by weight, relative to the total weight of the composition.

[0089] On the one hand, this disclosure relates to the application of an effective amount of at least one flower extract of one or more species of lotus or a composition containing the same to inhibit or block the release of acetylcholine at the NMJ via nerve cells, thereby relaxing and / or decontracting subcutaneous muscles, particularly facial expression muscles, to smooth the skin or eliminate wrinkles, wherein the inhibition of acetylcholine release at the NMJ via nerve cells corresponds to muscle, preferably facial expression muscles, relaxing or decontracting.

[0090] In another aspect, this disclosure covers a method for treating hyperhidrosis or drooling, the method comprising applying to the skin, in a cosmetically and / or dermatologically acceptable culture medium, a composition containing flower extracts of one or more species of lotus, in an amount effective in treating, reducing, preventing, and / or improving hyperhidrosis or drooling. The application of the composition containing flower extracts of one or more species of lotus is preferably topical.

[0091] Another aspect of this disclosure relates to a method for improving the aesthetic appearance of skin, comprising applying to the skin in an amount that effectively improves the aesthetic appearance of the skin, or introducing, via a targeted delivery mode, a composition comprising one or more flower extracts of one or more species, including lotus. According to this aspect, the aesthetic appearance relates to treating at least one condition, such as signs of dermatological aging. Dermatological aging can include aging over time, photoaging, hormonal aging, or any combination thereof.

[0092] As practitioners will understand, cosmetic treatments comprising compositions containing flower extracts of one or more species of lotus as described herein can be performed, for example, by topical application of the cosmetic composition as described herein, according to conventional techniques for applying such compositions. Conventional and commonly used techniques encompass the application of creams, lotions, gels, serums, ointments, antiperspirants, or deodorants to the skin; spraying as a form of application is also contemplated.

[0093] In addition to the active ingredients, the disclosed cosmetic compositions may include a sebaceous regulator ingredient that can act in conjunction with a traction solution of Egyptian blue water lily extract and Chinese lotus extract (“NELUPURE”). In the formulations disclosed herein, NELUPURE may be present at a concentration as low as 0.2 wt%. In NELUPURE, the nymphaea extract comprises about 0.5 wt% of NELUPURE, and the lotus extract comprises about 1 wt% of NELUPURE. In cosmetic compositions or formulations including NELUPURE, the nymphaea extract and / or lotus extract may independently range from about 0.0005 wt% to about 0.005 wt%, about 0.001 wt% to about 0.002 wt%, less than, equal to, or greater than about 0.0005 wt%, 0.001 wt%, 0.0015 wt%, or about 0.002 wt%. In addition to the extracts of Egyptian blue water lily and Chinese lotus, the extract solution may also include other components. These other components may include propylene glycol and glycerin. In some instances, the Egyptian blue water lily extract is present in the following ranges: about 0.00075 wt% to about 0.04 wt%, 0.001 wt% to about 0.02 wt%, 0.001 wt% to about 0.01 wt%, about 0.005 wt% to about 0.009 wt%, less than, equal to, or greater than about 0.001 wt%, 0.005 wt%, 0.009 wt%, or 0.01 wt%. The Chinese lotus extract may be in the following ranges: about 0.00075 wt% to 0.04 wt%, about 0.001 wt% to 0.02 wt%, about 0.001 wt% to about 0.01 wt%, about 0.005 wt% to about 0.009 wt%, less than, equal to or greater than about 0.001 wt%, 0.005 wt%, 0.009 wt% or 0.01 wt%. Propylene glycol may be present in the following ranges: about 0.08 wt% to about 4 wt%, about 0.1 wt% to 1.6 wt%, 0.5 wt% to about 1.5 wt%, about 0.6 wt% to about 1 wt%, less than, equal to or greater than about 0.5 wt%, 0.6 wt%, 0.7 wt%, 0.8 wt%, 0.9 wt%, 1.0 wt%, 1.1 wt%, 1.2 wt%, 1.3 wt%, 1.4 wt% or about 1.5 wt% of the extract.Glycerin may be present in the following ranges: about 0.01 wt% to about 1 wt%, about 0.03 wt% to 0.4 wt%, about 0.05 wt% to about 0.2 wt%, about 0.07 wt% to about 0.1 wt%, less than, equal to or greater than about 0.05 wt%, 0.06 wt%, 0.07 wt%, 0.08 wt%, 0.09 wt%, 0.1 wt% or about 0.2 wt%.

[0094] In addition to those disclosed herein, other examples of formulations including NELUPURE can be found in US20240299262A1 and US20240252424A1, the contents of which are incorporated herein by reference. Examples of sebum-regulating ingredients include mixtures of glycerin and water with palmitoyl pentapeptide-4 (trade name BB-BIONT), which are known to help reduce biofilm formation of Propionibacterium acnes and may help reduce acne scars. Other sebum regulators include zinc, zinc PCA, zinc gluconate, zinc oxide, lentil (Lens esculenta or Lentil) seed extract, syringa vulgaris extract, glycerin and water, a mixture of lemon myrtle leaf extract and backhousia citriodora leaf extract, octyl dodecanool and a mixture of twig / leaf oil of leptomeria (Leptospermum Scoparium) and black pepper (pepper) seed extract and magnolia officinalis bark extract, salicylic acid, salix alba extract, Quillaja saponaria bark extract, Mediterranean cypress (Cupressus Sempervirens) fruit extract, mimosa (Mimosa Tenuiflora) bark extract, butylene glycol and a mixture of 10-hydroxydecanoic acid and succinic acid, niacinamide, water and niacinamide and yeast extract, and horse chestnut (Aesculus hippocastanum) (horse chestnut). A mixture of Chestnut seed extract and glycyrrhizic acid, panthenol, propylene glycol, zinc gluconate, caffeine, and biotin; a mixture of Eucalyptus globulus and Pimpinella anisum seed extracts; Rosa centifolia flower extract; Paeonia suffruticosa root extract; Bombax malabaricum flower extract; Pistacia lentiscus gum / Boswellia suffruticosa gum and lecithin and glycerin and alcohol and water (Aqua / Water); Laminaria japonica, isopentyl glycol, and Trifolium pratense (clover) flower extract; Cucurbita pepo, Cinnamomum cassia bark extract, Zingiber officinale (or ginger) root extract, and Sanguisorba officinalis (burnt cinnamon) root extract. A mixture of *Salvia officinalis* root extract and sage (Salvia officinalis) root extract.Extracts of *Officinalis* or *Sage*, retinol, retinoic acid, retinyl derivatives, a mixture of *Plantago lanceolata* leaf and *Berberis aquifolium*, *Hypericum perforatum*, *Serenoa serrulata* fruit extract, and a mixture of *Sesamum indicum* / *Sesame* seed oil and *Argania spinosa* kernel oil and β-sitosterol and tocopherol, *Spiraea ulmaria* extract, *Crambe abyssinica* seed oil and a mixture of *Vitis vinifera* and *iris fiorentina* extracts, a mixture of propylene glycol and *Humulus lupulus* or *Hops* extract, and *Hamamelis virginiana* (witch hazel). A mixture of hazel and water, water and HCl, pyridoxine and nicotinamide and glycerol and panthenol and hydrolyzed yeast protein and threonine and allantoin and biotin, green tea, which is a mixture of tea tree (Camellia Sinensis) leaf extract and ivy (Hedera Helix or Ivy) leaf / stem extract and hydrolyzed soybean protein and Enteromorpha Compressa extract and Butyl avocadoate and tocopherol and potassium azeloyl diglycinate, Moringa pterygium seed extract and sarcosine and Cinnamomum zeylanicum bark extract and Houttuynia cordata and Crithmum maritimum extract and Ylang-ylang flower extract and a mixture of linum usitatissinum and Verbena officinalis, Lactobacillus fermentum A mixture of fermented, magnesium aluminum silicate and hydroxyethyl cellulose with black cumin (Nigella Sativa) seed oil and zucchini (pumpkin) seed oil and phospholipids; a mixture of butylene glycol and barosma betulina leaf extract and citric acid; a mixture of North African cedar (Cedrus atlantica) bark extract and butylene glycol and barosma betulina leaf extract and citric acid; and orthosiphon (cat's whiskers).A mixture of *Stamineus* leaf extract and water, maltodextrin, and wanthan gum; butylene glycol and a mixture of *Enantia Chlorantha* bark extract and oleanolic acid; a mixture of *Melia azadirachta* leaf and *Arctium lappa* root; sarcosine and a mixture of Ceylon cinnamon bark extract and kojic acid; *Bambusa Arundinacea* stem extract; farnesyl acetate, panthenol triacetate, tocopheryl acetate, and salicylic acid; a mixture of white willow bark and *Prunus armeniaca*; *Cassia italica* and *Saponaria officinalis*; sandalwood and *Laminium album*; *Plantago major*; *Myrtus communis*; *Originium marjorana*; lysine, carboxymethyl cysteine, and lysine; thiazoline and *Alchemilla*. A mixture of *Vulgaris* extract and *Harpagophytum procubens* root extract and diacid and glycolic acid and lactic acid and mandelic acid and gluconic acid and lactobionic acid and algae extract and *Laminaria saccharina* extract and acetylglucosamine and juniper alcohol and *Acacia senegal* gum and *Melaleuca alternifolia* leaf oil, capryloyl salicylic acid, chlorhexidine digluconate and piroctone olamine and carnosine and azelaic acid and sulfur and calamine and witch hazel extract and silymarin and ferulic acid, L-ascorbic acid and benzoyl peroxide and tartaric acid and citric acid, charcoal, kaolin or a mixture thereof.

[0095] In addition to the active ingredient, the disclosed cosmetic composition may contain any number of other ingredients, such as, but not limited to: additional active ingredients (e.g., cosmetic, dermatological and / or pharmaceutical), alcohols, allergy inhibitors, amino acids, anti-acne agents (e.g., salicylic acid), anti-aging agents, antibacterial agents, antifungal agents, analgesics, anti-hair loss agents, anti-wrinkle agents, antibacterial agents, antimicrobial agents, antioxidants, anti-inflammatory agents, burn healing agents, colorants (e.g., lakes, pigments), decolorizing agents, deodorants, dyes, emollients (e.g., glycerin, butylene glycol), excipients, fatty substances, fillers, film-forming agents (e.g., dimethicone copolymer, ethylhexyl acrylate copolymer), and fragrances. Free radical scavengers, glycerin, glyceryl monostearate, glyceryl distearate, hair growth agents, hair conditioners, hair softeners, hair moisturizers, herbal extracts, humectants (e.g., hyaluronic acid, orotic acid, lipoproteins), insect repellents, pharmaceuticals, moisturizers, non-active carrier oils (e.g., triglycerides, silicone oils, mineral oils), oils, peptides, polypeptides, proteins, fragrances, pigments, preservatives, plasticizers, reflectors, sebum absorbers, skin brighteners, sunscreens, surfactants, tanning agents, thickeners (hydroxyethyl cellulose, xanthan gum, carbomer), petrolatum, vasoconstrictors, vasodilators, vitamins (e.g., vitamin A, vitamin E), water, waxes, and / or combinations thereof.

[0096] In addition to the active ingredient, the disclosed cosmetic composition may contain any number of other ingredients, such as, but not limited to: additional active ingredients (e.g., cosmetic, dermatological and / or pharmaceutical), alcohols, allergy inhibitors, amino acids, anti-acne agents (e.g., salicylic acid), anti-aging agents, antibacterial agents, antifungal agents, analgesics, anti-hair loss agents, anti-wrinkle agents, antibacterial agents, antimicrobial agents, antioxidants, anti-inflammatory agents, burn healing agents, colorants (e.g., lakes, pigments), decolorizing agents, deodorants, dyes, emollients (e.g., glycerin, butylene glycol), excipients, fatty substances, fillers, film-forming agents (e.g., dimethicone copolymer, ethylhexyl acrylate copolymer), and fragrances. Free radical scavengers, glycerin, glyceryl monostearate, glyceryl distearate, hair growth agents, hair conditioners, hair softeners, hair moisturizers, herbal extracts, humectants (e.g., hyaluronic acid, orotic acid, lipoproteins), insect repellents, pharmaceuticals, moisturizers, non-active carrier oils (e.g., triglycerides, silicone oils, mineral oils), oils, peptides, polypeptides, proteins, fragrances, pigments, preservatives, plasticizers, reflectors, sebum absorbers, skin brighteners, sunscreens, surfactants, tanning agents, thickeners (hydroxyethyl cellulose, xanthan gum, carbomer), petrolatum, vasoconstrictors, vasodilators, vitamins (e.g., vitamin A, vitamin E), water, waxes, and / or combinations thereof.

[0097] Sunscreen ingredients may include zinc oxide, titanium dioxide, octinoxate, ensolizole, ethylhexyl triazine, octyl salicylate, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyl triazine, isoamyl p-methoxycinnamate, diethylhexylbutamidotriazine, or mixtures thereof. In some aspects, sunscreen ingredients may be octocrylene-free. Furthermore, according to some aspects, the sunscreen formulations described herein are eco-compatible compositions. For example, sunscreen formulations are biocompatible and harmless to marine ecosystems, particularly tropical environments with coral reefs. Specifically, sunscreen formulations do not contain components that inhibit cell growth or cell replication in the marine environment. In some instances, this can be achieved with the following sunscreen formulations: bis(ethylhexyloxyphenol) methoxyphenyl triazine (BEMT), cinnamic acid esters, benzophenone, camphor derivatives, titanium dioxide, tea tree oil, ylang-ylang flower extract, Bambousa Arundinacea extract, shea butter, animal-derived fats, ali root oil, tocopheryl acetate, tocopherol, retinol, polyphenolic compounds extracted from green tea, or mixtures thereof.

[0098] The compositions disclosed herein may also include other cosmetic ingredients, such as, but not limited to, humectants, emollients, moisturizers, anti-wrinkle ingredients, concealers, matte polishes, pigments, colorants, proteins, antioxidants, contour powders, chelating agents, emulsifiers, ultraviolet (UV) absorbers, oil absorbers, antifoaming agents, anti-sticking agents, thickeners, fragrances, preservatives, antimicrobial agents, antifungal agents, neutralizers, vitamins, plasticizers, cohesives, alkalizing and acidifying agents, fillers, solvents, and mixtures thereof.

[0099] The composition may contain additional ingredients such as alkalizing agents, emulsifiers, emollients, plasticizers, preservatives, humectants, moisturizers, solvents, and tensile agents, or active ingredients suitable for providing anti-aging benefits. Examples of preferred additional ingredients include glycerin.

[0100] Other ingredients may optionally be added to the disclosed compositions, as detailed below.

[0101] Colorants or pigments: The composition may comprise one or more cosmetic powders, such as calcium aluminum borosilicate, PMMA, polyethylene, polystyrene, methyl methacrylate crosspolymer, nylon-12, ethylene / acrylic acid copolymer, boron nitride, Teflon, silica, etc. Typically, the composition will include colorants or pigments to impart a desired color or effect; examples include inorganic pigments, organic pigments, and / or lakes. Exemplary inorganic pigments include, but are not limited to, metal oxides and metal hydroxides, such as magnesium oxide, magnesium hydroxide, calcium oxide, calcium hydroxide, aluminum oxide, aluminum hydroxide, iron oxides (α-Fe₂O₃, γ-Fe₂O₃, Fe₃O₄, FeO), red iron oxide, yellow iron oxide, black iron oxide, iron hydroxide, titanium dioxide, lower titanium oxides, zirconium oxide, chromium oxide, chromium hydroxide, manganese oxide, cobalt oxide, cerium oxide, nickel oxide, and zinc oxide, as well as complex oxides and complex hydroxides, such as iron titanate, cobalt titanate, and cobalt aluminate. Non-metallic oxides such as aluminum oxide and silicon dioxide, ultramarine (e.g., sodium aluminum silicate containing sulfur), Prussian blue, manganese violet, bismuth oxychloride, talc, mica, sericite, magnesium carbonate, calcium carbonate, magnesium silicate, magnesium aluminum silicate, silicon dioxide, titanate mica, iron oxide titanate mica, bismuth oxychloride, etc., are also considered as suitable inorganic pigments. Organic pigments may include, but are not limited to, carbon black, carmine, phthalocyanine blue and green pigments, diaryl yellow and orange pigments, and azo red and yellow pigments such as toluidine red, lithol red, naphthol red, and brown pigments, and at least one combination thereof.

[0102] A lake is generally a colorant prepared from a water-soluble organic dye (e.g., D&C or FD&C) that has precipitated onto an insoluble reactive or adsorbent matrix or diluent. As used herein, the term "D&C" refers to FDA-approved pharmaceutical and cosmetic colorants for use in pharmaceuticals and cosmetics. As used herein, the term "FD&C" refers to FDA-approved food, pharmaceutical, and cosmetic colorants for use in food, pharmaceuticals, and cosmetics. Certified D&C and FD&C colorants are listed in 21 CFR §74.101 and include FD&C colors Blue 1, Blue 2, Green 3, Orange B, Orange-red 2, Red 3, Red 4, Red 40, Yellow 5, Yellow 6, Blue 1, Blue 2, Orange B, Orange-red 2, and D&C colors Blue 4, Blue 9, Green 5, Green 6, Green 8, Orange 4, Orange 5, Orange 10, Orange 11, Red 6, Red 7, Red 17, Red 21, Red 22, Red 27, Red 28, Red 30, Red 31, Red 33, Red 34, Red 36, Red 39, Violet 2, Yellow 7, Yellow 8, Yellow 10, Yellow 11, Blue 4, Blue 6, Green 5, Green 6, Green 8, Orange 4, Orange 5, Orange 10, Orange 11, etc. Suitable substrates for forming lakes include, but are not limited to, mica, bismuth oxychloride, sericite, alumina, aluminum, copper, bronze, silver, calcium, zirconium, barium, as well as strontium, titanium mica, filmed silica, spherical silica, polymethyl methacrylate (PMMA), micronized Teflon, boron nitride, acrylate copolymers, aluminum silicate, aluminum starch octenyl succinate, bentonite, calcium silicate, cellulose, chalk, corn starch, diatomaceous earth, bleaching clay, and glycerol. Oil starch, hyaluronate, hydrated silica, kaolin, magnesium aluminum silicate, magnesium trisilicate, maltodextrin, montmorillonite, microcrystalline cellulose, rice starch, silica, talc, mica, titanium dioxide, zinc laurate, zinc myristate, zinc rosinate, alumina, ahapulgite, calcium carbonate, calcium silicate, dextran, nylon, silica silyl alkylates, silk powder, sericite, soybean flour, tin oxide, titanium hydroxide, magnesium triphosphate, walnut shell powder, and mixtures thereof. Suitable lakes include, but are not limited to, lakes of red dyes from the monoazo, diazo, Norm, xanthan, or indigo families, such as Red 4, 6, 7, 17, 21, 22, 27, 28, 30, 31, 33, 34, 36, and Red 40; lakes of yellow pyrazole, monoazo, fluorescein, xanthan, quinoline, dyes, or their salts, such as Yellow 5, 6, 7, 8, 10, and 11; lakes of purple dyes, including purple dyes from the anthraquinone family, such as Violet 2; and lakes of orange dyes, including Orange 4, 5, 10, and 11. Suitable lakes of D&C and FD&C dyes are defined in 21 CFR § 82.51.

[0103] The colorant may optionally be surface-treated, for example, to make the particles more hydrophobic or more dispersible in the medium. The surface of the particles may be covalently or ionicly bound to organic or silicon-based molecules, or may be adsorbed onto organic or silicon-based molecules, or the particles may be physically coated with a material layer. The surface-treated compound may be attached to the particles by any suitable coupling agent, linker group, or functional group (e.g., silane, ester, ether, etc.). The compound may contain a hydrophobic portion selected from, for example, alkyl, aryl, allyl, vinyl, alkyl-aryl, aryl-alkyl, organosilicon, diorganosilicon, dimethicone, methyl methacrylate, polyurethane, silicone-polyurethane, and their fluorine or perfluorinated derivatives. Other hydrophobic modifiers include lauroyl lysine, isopropyltrisethiotetra(ITT) isopropyltrisethiotetra(ITT), ITT and polydimethicone (ITT / dimethicone) crosspolymers, ITT and amino acids, ITT / triethoxyoctanoyl silane crosspolymers, waxes (e.g., carnauba palm), fatty acids (e.g., stearates), HDI / trimethylolcaprolactone crosspolymers, and PEG-8 methyl ether triethoxysilane, aloe vera, jojoba esters, lecithin, perfluorophosphates, and magnesium myristate (MM).

[0104] Optional pigment components include alkylsilane-treated colorants, which essentially consist of or comprise an alumina substrate (e.g., flaky) and a pigment, dye, or lake bonded to the alumina substrate by an alkylsilane surface treatment. Typically, the alkyl viscous liquid will be octylsilane and can be formed by treatment with triethoxyoctanoylsilane. Non-limiting examples of such colorants include, but are not limited to, 1% aluminum oxide / titanium dioxide / triethoxyoctanoyl silane (COVALUMINE Atlas White AS), 1% aluminum oxide / D&C red aluminum lake CTD / triethoxyoctanoyl silane (COVALUMINE Red Rose AS), 1% aluminum oxide / D&C red aluminum lake CTD / triethoxyoctanoyl silane (COVALUMINE Sonoma Red AS), 1% aluminum oxide / black iron oxide CTD / triethoxyoctanoyl silane (COVALUMINE Sonoma Black AS), 1% aluminum oxide / D&C red #6 aluminum lake CTD / triethoxyoctanoyl silane (COVALUMINE Fire Red AS), 1% aluminum oxide / yellow I iron oxide CTD / triethoxyoctanoyl silane (COVALUMINE Sonoma Yellow AS), and 1% aluminum oxide / D&C blue #1 aluminum lake CTD / triethoxyoctanoyl silane (COVALUMINE Astral Blue). The following are available from SENSIENT Cosmetic Technologies LCW: Alumina / Carmine CTD / Triethoxyoctanoylsilane 1% (COVALUMINECampari AS), Alumina / Yellow #5 CTD / Triethoxyoctanoylsilane 1% (COVALUMINE Sunburst AS), Alumina / Triethoxyoctanoylsilane 1% and combinations thereof.

[0105] It may also include interference or pearlescent pigments. These typically comprise mica layered with a film of about 50 to 300 nm, such as TiO2, Fe2O3, or Cr2O3. These include white pearlescent materials, such as mica coated with titanium dioxide or bismuth oxychloride; and colored pearlescent materials, such as titanium mica coated with iron oxide, titanium mica coated with iron blue or chromium oxide, or titanium mica coated with organic pigments of the aforementioned types. If these materials are used, it is preferred that they are used together in an amount of less than 1.0 wt%. Preferably, the pearlescent component has a pearlescent composition or reflective pearl based on bismuth oxychloride. Bismuth oxychloride matches the natural pearlescent luster of the skin better than compounds that provide a more artificial appearance, such as titanium dioxide. Bismuth oxychloride better mimics the natural reflection of the skin. However, other pearlescent components can be used. A preferred pearlescent component is called CHROMA-LITE, which is a combination of colored pigments bonded to BI-LITE 20 (bismuth oxychloride and mica) using calcium stearate. CHROMA-LITE components are available in various hues / colors from Englehard Corporation (Iselin, NJ).

[0106] Cosmetic-acceptable mediators: The disclosed compositions will generally contain cosmetic-acceptable mediators. As used herein, "cosmetic-acceptable" means safe for contact with the human exodermis. The mediator may comprise a liquid, including a single-phase, two-phase system, or an emulsion. Emulsions include oil-in-water, silicone-in-water, water-in-oil, and silicone-in-water emulsions. When formulated into an emulsion, an emulsifier is typically included. Specifically, it may be made from volatile silicones (e.g., cyclopentasiloxane), hydrocarbons, ester oils, lower alcohols (e.g., ethanol, isopropanol, etc.) and water.

[0107] Emollients: Emollients may be used in this disclosure and include, but are not limited to, ester oils, which will generally be in the form R4(COOH). 1-2 The acid and its form R5(OH) 1-3 The etherified product of an alcohol, wherein R4 and R5 are each independently a linear, branched, or cyclic hydrocarbon group, optionally containing an unsaturated bond, and having 1 to 30 carbon atoms, preferably 2 to 30 carbon atoms, and more preferably 3 to 30 carbon atoms, optionally substituted with one or more functional groups including hydroxyl, oxa, oxo, etc. Preferably, at least one of R4 and R5 contains at least one aliphatic chain. Esters as defined above include, but are not limited to, esters of monoacids having a monool, monoacids having a diol and a triol, diacids having a monool, and tricids having a monool. Other emollients include dimethicone. Humectants such as glycerin and other C1-10 polyols or diols may also be included.

[0108] Adsorbent Powder: Adsorbent powders that can absorb oil can be added to improve comfort. Examples of suitable adsorbent powders may include silica, mica, elastomers, kaolin, starch, or mixtures thereof.

[0109] Filler: Filler may also be added optionally in an amount of about 1% to about 20% by weight, preferably about 1% to about 10% by weight, of the final composition. Examples of fillers include, but are not limited to, silica, PMMA, nylon, alumina, barium sulfate, or any other filler commonly used in such compositions.

[0110] Film-forming agents: Polymer film-forming agents include cellulose, polyolefins, polyethylene, polyacrylates, polyurethanes, silicones, silicone acrylates, polyamides, polyesters, fluoropolymers, polyethers, polyacetates, polycarbonates, polyimides, rubbers, epoxy resins, formaldehyde resins, and homopolymers and copolymers of any of the foregoing. Specifically, when present, the film-forming agent may comprise from about 2 wt% to about 20 wt% of the active component, and from about 7 wt% to about 13 wt% of the active component, less than, equal to, or greater than about 2 wt%, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or about 20 wt%. As described above, film-forming agents can be used to help produce a homogeneous mixture of the extract solution and to help distribute the composition evenly on the user's skin. Depending on the specifics, the film-forming agent may include acrylate copolymers, acrylate copolymers (and) acrylate / polytrimethylsiloxane methacrylate copolymers, acrylate / octylacrylamide copolymers, acrylate / C1-2 succinate / hydroxy acrylate copolymers, acrylate / C12-22 alkyl methacrylate copolymers, acrylate / dimethicone copolymers, acrylate / ethylhexyl acrylate copolymers, acrylate / hydroxy ester acrylate copolymers, acrylate / lauryl acrylate / stearyl acrylate / ethylamine methacrylate copolymers, acrylate / octylacrylamide copolymers, acrylate / polytrimethylsiloxane methacrylate copolymers, acrylate / polytrimethylsiloxane methacrylate copolymers (and) lauryl ether-1 phosphate, acrylate / tert-butylacrylamide copolymers, adipic acid / diethylene glycol crosspolymers, adipic acid / neopentyl glycol / trimethicone copolymers, alginate octenyl succinate aluminum starch AMP-acrylate copolymers, and so on. 2,2-dimethyl methacrylate / tert-butyl methacrylate copolymer, rapeseed / tung oil copolymer, butyl acrylate / hydroxypropyl dimethicone acrylate copolymer, PVM / MA copolymer butyl ester, C24-28 alkyl dimethyl siloxytrimethyl siloxy silicate, octanoyl glycerol / sebacic acid copolymer, diisostearoyl polyglycerol-3 dimer linoleate, PVM / MA copolymer ethyl ester, isobutylene / ethyl maleimide / hydroxyethyl maleimide copolymer, PVM / MA copolymer isopropyl ester, methyl methacrylate, tert-butyl ... Acryloyl ethyl betaine / acrylate copolymer, octyl acrylamide / acrylate / butylamino methacrylate copolymer, phenylpropyl dimethylsiloxysilicate, polyamide-3, polyamide-8, polyester-10, polyester-7, polyethylene, polymethylsilsesquioxane, polyurethane-1, polyurethane-14 (and) AMP-acrylate copolymer, polyurethane-18, polyurethane-34, polyurethane-35, polyurethane-48, polyurethane-64, polyurethane-93, polyvinyl stearyl ether,PPG-17 / IPDI / DMPA copolymer, PPG-26 / dimer-linoleate copolymer, pullulan, PVP, PVP crosspolymer, sodium polyacrylate, sodium polystyrene sulfonate, styrene / acrylate copolymer, styrene / VP copolymer, triacontyl PVP, trifluoropropyl dimethylsiloxy / trimethylsiloxy polysilsesquioxane, trimethylpentanediol / adipic acid / glycerol crosspolymer, trimethylsiloxy silicate, VA / butyl, maleate / isobornyl acrylate copolymer, VA / crotonate copolymer VA / crotonate / vinyl neodecanoate copolymer, vinylcaprolactam / VP / dimethylaminoethyl methacrylate copolymer, VP / hexadecene copolymer, VP / acrylate / laurate methacrylate copolymer, VP / dimethylaminoethyl methacrylate copolymer, VP / DMAPA acrylate copolymer, VP / eicosene copolymer, VP / hexadecene copolymer, VP / methacrylamide / vinylimidazolium copolymer, VP / VA copolymer, VP / vinylcaprolactam / DMAPA acrylate copolymer, copolymers thereof, or mixtures thereof. In a specific aspect, the film-forming agent may include sodium polyacrylate.

[0111] Oils: Suitable non-limiting examples of oils in the oil phase (e.g., in an emulsion) include natural and synthetic oils, including animal fats, vegetable oils, and petroleum; fatty acid triglycerides; fatty acid esters, such as octyl palmitate, isopropyl myristate, and isopropyl palmitate; ethers, such as dioctyl ether; fatty alcohols, such as cetyl alcohol, stearyl alcohol, and behenyl alcohol; sterols; hydrocarbons such as isooctane, isododecane, isohexadecane, decane, dodecane, tetradecane, tridecane, C 8-20 Isoparaffins, mineral oils, petrolatum, isoeicosane, and polyisobutylene; C 10-30 Cholesterol / lanosterol esters; lanolin, etc. Representative hydrocarbons include paraffinic hydrocarbons available from ExxonMobil under the trademark ISOPARS and from Permethyl Corporation. Additionally, C... 8-20 Paraffinic hydrocarbons, such as those manufactured by Permethyl under the trademark PERMETHYL 99ATM. 12 Isoalkanes (isododecane) are suitable. Commercially available C... 16 Isoparaffins, such as isohexadecane (with the trademark name PERMETHYL.RTM.), are also suitable. Silicone oils, such as dimethicone, cyclic silicones, and polysiloxanes, can also be included in the oil phase. On the one hand, the silicone oil is present in an amount of less than about 5% by weight in the oil phase.

[0112] Solvents: Cosmetic agents can contain volatile solvents. Typically, volatile solvents can have a vapor pressure above about 0.01 mmHg at 20°C. Volatile solvents may include volatile C 5-12 Hydrocarbons (e.g., isododecane), aromatic hydrocarbons (e.g., xylene, toluene, etc.), ketoneacetone, methyl ethyl ketone, etc.), ethers (e.g., diethyl ether, methyl ethyl ether, etc.), perfluorocarbons, hydrofluoroethers, Freons, acetates (e.g., ethyl acetate, butyl acetate, etc.), etc. Preferred volatile solvents will be cosmetically acceptable. The solvent may be an organic solvent. When present, the solvent comprises about 10 wt% to about 50 wt% of the active ingredient, about 30 wt% to about 40 wt% of the active ingredient, and less than, equal to, or greater than about 30 wt%, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or about 50 wt% of the composition. Examples of organic solvents may include isododecane components, alkyl benzoate components, and neopentyl glycol diheptanoate components. Isododecane (examples thereof are represented by CAS 31807-55-3) is generally understood as a transparent, colorless, and odorless volatile liquid, making it suitable for use in makeup products such as mascara, eyeliner, lip products, or any product requiring improved abrasion properties and without the need for residue. It does not leave oily residue. Isododecane is a volatile lipophilic component used in deodorant sprays and hair care applications. It is a hydrocarbon component used as a solvent. Alkyl benzoate components can be C12-15 alkyl benzoates (examples thereof are represented by CAS 68411-27-8), which is generally understood as a low molecular weight ester of benzoic acid and C12-15 alcohol. C12-15 alkyl benzoates are nearly odorless, transparent liquids. In some instances, alkyl benzoate components include C12 alkyl benzoates, C13 alkyl benzoates, C14 alkyl benzoates, C15 alkyl benzoates, or mixtures thereof. Neopentyl glycol diheptanoate (an example thereof is represented by CAS 68855-18-5) is generally understood as a mixture of the texture-enhancing ingredient neopentyl glycol and the grape-derived fatty acid heptacid.

[0113] According to various aspects of this disclosure, the compositions are substantially free of cyclopentasiloxane. For example, the compositions may include less than about 10 wt% cyclopentasiloxane, less than about 9 wt%, less than about 8 wt%, less than about 7 wt%, less than about 6 wt%, less than about 5 wt%, less than about 4 wt%, less than about 3 wt%, less than about 2 wt%, less than about 1 wt%, less than about 0.5 wt%, less than about 0.1 wt%, less than about 0.01 wt%, or less than about 0.001 wt%. In some aspects, the compositions are completely free of (e.g., include 0 wt%) cyclopentasiloxane. The disclosed isododecane component, alkyl benzoate component, and neopentyl glycol diheptanoate component together serve as an effective substitute for cyclopentasiloxane while having similar, equal, or superior properties. Substitution for cyclopentasiloxane may be desirable because, despite its performance in the composition, the modifier seeks to limit the use of cyclopentasiloxane in cosmetic products. Therefore, finding effective alternatives to cyclopentasiloxane is important. Suitable alternatives may include oleochemical components such as dioctyl carbonate, dioctyl ether, or mixtures thereof.

[0114] The surface of a single pigment component, either entirely or partially, can be coated. Examples of suitable coatings include lauroyl lysine coatings, silane coatings, silicone oil coatings, dimethicone oil coatings, stearoyl glutamic acid, or combinations thereof. As commonly understood, lauroyl lysine is an amino acid derivative derived from the natural product lysine, namely protein and lauric acid, another natural coconut fatty acid. Lauroyl lysine treatment produces a hydrophobic system with enhanced dispersibility, increased abrasion properties, and a moisturizing feel on the skin in cosmetics. Compared to silane treatment, the pigment surface is smooth, flat, and less fuzzy, making it easier to press. Lauroyl lysine coatings are lubricating, thus imparting a smooth, moist feel to the substrate. As commonly understood, silane coatings are deposition achieved through the reaction of silanols with the pigment. By hydrolyzing the "Si-OR" group to silanol (Si-OH), alkylsilanes can self-associate or hydrogen-bond to the pigment surface, thus forming stable bonds between the pigment and the treated compound after curing. Silicone-treated pigments disperse well in cyclomethicone oils. Silicone has a very low surface tension, resulting in excellent hydrophobicity and better lipophilicity, as it disperses easily in mineral oils, esters, and silicone fluids. Pigments treated with alkylsilanes are more hydrophobic than those treated with silicone oils, are more wettable in commonly used cosmetic oils, and have lower oil absorption. In hydrated, dense formulations, the excellent wetting properties imparted by alkylsilane treatment allow for high pigment loading to achieve a “powdered” feel when applied to the skin, while maintaining a low melt viscosity for heat filling. As commonly understood, silicone oil coatings are polymethylhydrosiloxanes that absorb trace amounts of water from the pigment surface and convert Si-H bonds to Si-OH upon the release of a small amount of hydrogen, forming covalent Si-O pigment chemical bonds upon curing. The resulting coating is highly hydrophobic and not soluble in common solvents. The pigment is fully wetted in the oil, specifically in the silicone oil. The skin feels slightly dry, with enhanced slip and diffusivity. As commonly understood, dimethicone coatings (also known as polymethylsiloxanes) are coatings that bond to the surface of pigments or fillers through a mechanism of hydrolysis, condensation, and curing to Si-O pigment bonds. Surfaces treated with dimethicone are quite hydrophobic and offer good slip and a more lubricated feel. It can be used in oil-based systems, which can be used with anhydrous products.

[0115] Regarding individual pigments, the coating can essentially cover 100% of the total surface area of ​​the individual pigment. In some other aspects, the coating can cover approximately 5% to approximately 95% of the total surface area of ​​the individual pigment, approximately 10% to approximately 90% of the total surface area, approximately 15% to approximately 20% to approximately 80% of the total surface area, approximately 25% to approximately 75% of the total surface area, approximately 30% to approximately 70% of the total surface area, approximately 35% to approximately 65% ​​of the total surface area, approximately 40% to approximately 60% of the total surface area, approximately 45% to approximately 50% of the total surface area, less than, equal to, or greater than approximately 5% of the total surface area, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, or 24% of the total surface area. 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or about 95%. In some respects, at least a portion of the total amount of pigment present may be substantially free of any coating.For example, when less than 100% of the total pigment includes a coating, approximately 5% to approximately 95% of the total pigment may include a coating, approximately 10% to approximately 90% of the total pigment may include a coating, approximately 15% to approximately % of the total pigment may include a coating, approximately 20% to approximately 80% of the total pigment may include a coating, approximately 25% to approximately 75% of the total pigment may include a coating, approximately 30% to approximately 70% of the total pigment may include a coating, approximately 35% to approximately 65% ​​of the total pigment may include a coating, approximately 40% to approximately 60% of the total pigment may include a coating, approximately 45% to approximately 50% of the total pigment may include a coating, less than, equal to, or greater than approximately 5% of the total pigment may include a coating, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, and 22%. 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or approximately 95%. Each pigment may be coated with the same material, or different portions of the pigment may be at least partially coated with different coatings. An example of a suitable coating is stearoylglutamic acid.

[0116] In some examples where the coating is stearoylglutamic acid, the composition includes polyglycerol diisostearate, glyceryl stearate, or a combination thereof.

[0117] Thickeners: Suspending agents and thickeners typically include silica gel, gums, clay, pyrolytic silica, fatty acid soaps, and various hydrocarbon gels, as well as other components that remain on the surface of the stratum corneum when incorporated into formulations, as disclosed in the International Cosmetic Dictionary and Handbook (12th edition), which is hereby cited and incorporated. Thickening agents, such as gelling agents, may also be used. Examples include bentone, triglycerides, aluminum stearate, C18-C36 glycol esters, triglycerides, glyceryl monostearate, alginate, carbomer, cellulose, gums, carrageenan, starch, or silicates.

[0118] Waxes: Waxes that may be used in this disclosure include, but are not limited to, linear polyethylene, microcrystalline petroleum wax, cannawa wax, lignite wax, carnauba wax, rice bran wax, castor wax, mortar wax, stearyl ketone, synthetic fatty acid esters, myrica wax, castor wax, Japanese wax, ceresin wax, beeswax, candelilla wax, petrolatum, microcrystalline wax, cocoa butter, alpinia butter, thatch wax, shellac wax, glycol diester or triester of C18-C36 fatty acids, cetear, paraffin wax, hard animal fat oil, lanolin, lanolin alcohol, cetearyl alcohol, glyceryl monostearate, sugarcane wax, jojoba wax, stearyl alcohol, silicone wax, and combinations thereof.

[0119] In some aspects of this disclosure, the composition may include a preservative. Where present, the preservative may range from about 0.0001 wt% to about 0.5 wt%, from about 0.0003 wt% to about 0.1 wt%, less than, equal to, or greater than about 0.0001 wt%, 0.0003, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, or about 0.5 wt% of the composition.

[0120] When included, the preservative is included in an amount sufficient to prevent spoilage or the growth of unintentionally added microorganisms within a given time period, but insufficient to contribute to the odor-neutralizing properties of the composition. In other words, the preservative is not used as an antimicrobial compound to kill microorganisms deposited on the composition to eliminate odors produced by microorganisms. Instead, it is used to prevent spoilage of the composition to increase shelf life.

[0121] Preservatives can be any organic preservative material that does not damage the appearance of the fabric, such as causing discoloration, staining, or bleaching. Suitable water-soluble preservatives include organosulfur compounds, halogenated compounds, cyclic organonitrogen compounds, low molecular weight aldehydes, parabens, propylene glycol materials, isothiazolinones, tetroxides, benzoates, low molecular weight alcohols, dehydroacetic acid, phenyl and phenoxy compounds, or mixtures thereof.

[0122] Non-limiting examples of commercially available water-soluble preservatives include a mixture of about 77% 5-chloro-2-methyl-4-isothiazolin-3-one and about 23% 2-methyl-4-isothiazolin-3-one; a broad-spectrum preservative available from Rohm and Haas Co. under the trade name Kathon® CG in a 1.5% aqueous solution; and 5-bromo-5-nitro-1,3-dioxane available from Henkel under the trade name Bronidox. L®; 2-bromo-2-nitropropane-1,3-diol, available from Inolex under the trade name Bronopol®; 1,1'-hexamethylenebis(5-(p-chlorophenyl)biguanidine) (commonly known as chlorhexidine) and its salts, for example, having acetic acid and dichuronic acid; a 95:5 mixture of 1,3-bis(hydroxymethyl)-5,5-dimethyl-2,4-imidazolidinedione and 3-butyl-2-carbamate iodopropionyl ester, available from Lonza under the trade name Glydant Plus®; N-[1,3-bis(hydroxymethyl)2,5-dioxo-4-imidazolyl]-N,N'-bis(hydroxymethyl)urea, commonly known as diazolinyl urea, available from Sutton Laboratories, Inc. under the trade name Germall® II; N,N''-methylenebis{N'-[1-(hydroxymethyl)-2,5-dioxo-4-imidazolyl]urea}, commonly known as imidazolyl urea, is available from 3V-Sigma under the trade name Abiol®, from Induchem under the trade name Unicide U-13®, and from Sutton Laboratories under the trade name Germall 115®; polyoxymethylene bicyclooxazolidine, is available from Hüls America under the trade name Nuosept® C; formaldehyde; glutaraldehyde; polyaminopropyl biguanide, is available from ICI Americas under the trade name Cosmocil CQ® or from Brooks, Inc. under the trade name Mikrokill®; dehydroacetic acid; and benzothiazolinone, is available from Rohm and Haas under the trade name Koralone™ B-119.

[0123] The composition may further include an inactive component. The inactive component may comprise from about 50 wt% to about 90 wt%, from about 75 wt% to about 85 wt%, less than, equal to or greater than about 50 wt%, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89 or about 90 wt%. When an inactive component is present, the active component comprises, in the range of about 10 wt% to about 50 wt%, about 15 wt% to about 25 wt%, less than, equal to or greater than about 10 wt%, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or about 50 wt%.

[0124] On one hand, the composition is a pretreatment composition consisting of a non-tinted formulation containing an active ingredient, intended for application to the epidermis to reduce sebum before the application of a tinted cosmetic composition. Any portion of the above-described composition can be combined in any desired manner to produce a variety of different types of cosmetic compositions. The cosmetic composition can be applied to the epidermal layer of a user's skin. For example, the cosmetic composition can be a pretreatment formulation, a makeup formulation, a foundation, a sunscreen, or any other cosmetic applied to the user's epithelial tissue. Additionally, any portion of the foregoing composition can be combined to produce a cosmetic formulation that can be applied to the user's stratum corneum. For example, a cosmetic formulation can be a mascara, hair dye, nail polish, lip gloss, lipstick, or other tinted / colored cosmetic formulation intended for long-lasting compositions.

[0125] On the one hand, components derived from lotus plants (e.g., NELUPURE) can be formulated and / or used in products and / or programs to reduce perspiration in subjects. In some instances, perspiration reduction is primarily achieved without clogging pores.

[0126] On one hand, lotus-derived ingredients can be formulated and / or utilized in the form of a makeup primer to create a virtually flawless facial canvas, allowing cosmetics to last longer after application. In another embodiment, lotus-derived ingredients can be formulated and / or utilized in the form of a foundation composition. Suitable foundation formulations are those capable of evening out skin tone, concealing blemishes, and / or providing a flawless matte finish.

[0127] On the other hand, the composition derived from lotus plants can be formulated and / or utilized in the form of a cosmetic concealer, which essentially covers minor blemishes, dark spots, and dark circles.

[0128] On the other hand, ingredients derived from lotus plants can be formulated and / or utilized in combination cosmetic foundation / concealer products. Alternatively, ingredients derived from lotus plants can be formulated and / or utilized in the form of setting sprays, which can be sprayed over cosmetics to essentially lock in the makeup (after application) and protect the skin. Ingredients derived from lotus plants can also help reduce skin acne caused by cosmetics and sweat.

[0129] On one hand, the composition can be used to help treat rosacea. Patients with rosacea experience symptoms including persistent erythema and flushing, which are secondary manifestations caused by neurovascular changes. Reduction of these undesirable manifestations has been studied through administration of acetylcholine release. NELUPURE is believed to be effective for persistent erythema, rosacea, and general redness. Therefore, methods of treating rosacea may include the application of a composition comprising NELUPURE. The composition can be applied topically.

[0130] Example

[0131] The following examples are provided as illustrative examples of potential use and are derived from testing of formulations using sebum / sweat-reducing agents, such as NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract), compared to control formulations that do not use such agents. Various aspects of this disclosure can be better understood by referring to the following examples, which are provided as illustrative. This disclosure is not limited to the examples given herein.

[0132] Example 1: Consumer comparison of long-lasting foundation compositions

[0133] In the consumer testing survey, consumers will be asked to compare foundation compositions containing NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract) extract solution with commercially available transfer-resistant foundation compositions (standard) that do not contain NELUPURE (INCI: propylene glycol (and) glycerin (and) Egyptian blue water lily extract (and) Chinese lotus extract) extract solution.

[0134] In a double-blind test, each consumer tester will apply one layer of each foundation composition to one half of their clean (e.g., without any facial products) face (e.g., left side vs. right side). Consumers will be asked to compare their own sensations, comfort, appearance / coverage, and ease of removal at different times: immediately after application, 8 hours after application, and up to 9.9 hours after application, and to answer questions in a questionnaire comparing the two foundation compositions. Data will be tabulated using Compusense5 and analyzed using standard statistical processing procedures.

[0135] Expected consumer reviews from the aforementioned consumer tests indicate that, overall, the foundation composition containing NELUPURE (INCI: propylene glycol (and), glycerin (and), Egyptian blue water lily extract (and Chinese lotus extract) extract solution will provide a significantly more natural look after 8 hours of wear compared to other standard foundations. Consumers are expected to find the new foundation feels significantly lighter and more comfortable after 8 hours of application compared to standard foundations. Consumers are expected to find the foundation breathable and comfortable. Regarding appearance or coverage, consumers are expected to agree that the foundation evens out skin tone, reduces the appearance of fine lines and wrinkles, reduces redness, and reduces the appearance of age spots or sunspots. Overall, the foundation is expected to provide a natural and flawless look.

[0136] The technical evaluators, who are expected to oversee consumer testing, determined that the new foundation is more visible and provides greater coverage several hours after application compared to standard foundation. On average, the new foundation is expected to last at least 8 hours. The technical evaluators are expected to classify the new foundation composition as "long-lasting."

[0137] Example 2: Consumer testing of long-lasting foundation compositions

[0138] In the consumer testing survey, consumer testers will be asked to evaluate the foundation composition of the present invention containing an extract solution of NELUPURE (INCI: propylene glycol (and), glycerin (and), Egyptian blue water lily extract (and Chinese lotus extract)). Each consumer tester will apply a layer of the foundation composition to their clean face (e.g., without any facial products). Consumers will be asked to evaluate the feel, comfort, application, appearance, and coverage at different times: initially upon application and 8 hours after application, and to answer questions. The questionnaire will contain a series of questions about the aesthetics and performance of the foundation based on a seven-point attribute scale. Expert evaluators will also rate the appearance and wear characteristics at the initial time point and 8 hours after application, compared to a standard.

[0139] In general, the following results are expected: Consumers will like the composition both initially and after 8 hours of application. Consumers will find the foundation easy to apply and blend, providing even coverage and a smooth feel upon application. Consumers will find the composition initially lightweight; evens out skin tone; reduces the appearance of fine lines and wrinkles, redness, under-eye dark circles, age spots / sunspots, and shine; improves the appearance of skin texture; and provides a flawless and natural look. Even after 8 hours of application, the foundation is expected to be breathable and comfortable. It is expected to maintain smoothness, coverage, and color after 8 hours of application. The foundation composition is expected to still control shine and oiliness while looking freshly applied. After 8 hours, consumers are expected to find that the foundation has not settled in fine lines, wrinkles, or pores, nor does it appear clumpy or mask-like. Even if the foundation does not transfer after 8 hours, it is expected that the vast majority of consumers will find the foundation composition easy to remove.

[0140] Example 3: Inhibition of acetylcholine release at the neuromuscular junction

[0141] The aim of this study was to evaluate the effect of 0.2% NELUPURE (INCI: propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract) on the inhibition of acetylcholine release. The study was conducted using a specific neuromuscular co-culture model. In this model, human fibroblasts were reprogrammed into pluripotent cells and then differentiated into motor neurons. These motor neurons were co-cultured with human muscle cells to generate a functional neuromuscular junction. After incubation with 0.2% NELUPURE (INCI: propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract), the culture was activated with glutamate to induce the release of acetylcholine from the motor neurons. Following activation, the release of acetylcholine was quantified in the cell culture medium by an appropriate ELISA assay. Under these experimental conditions, a highly significant inhibition (p < 0.0001) of acetylcholine release was expected with 0.2% NELUPURE (INCI: propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract).

[0142] Example 4: Makeup primer composition

[0143] A makeup primer composition that may include NELUPURE may include the components shown in Table 1.

[0144] Table 1

[0145]

[0146] Example 4: Makeup primer composition

[0147] Makeup primer compositions including NELUPURE may include the components shown in Table 2.

[0148] Table 2

[0149]

[0150] Example 5: Makeup primer composition

[0151] Makeup primer compositions including NELUPURE may include the components shown in Table 3.

[0152] Table 3

[0153]

[0154] Table 4

[0155]

[0156] Table 5

[0157]

[0158]

[0159] Example 6: Makeup primer composition

[0160] Makeup primer compositions including NELUPURE may include the components shown in Tables 6A and 6B.

[0161] Table 6A

[0162]

[0163]

[0164] Table 6B

[0165]

[0166] Example 7: Including NELUPURE setting spray formulation

[0167] NELUPURE setting spray formulations may include the components shown in Table 7.

[0168] Table 7:

[0169]

[0170] Example 8: Testing the sebum control and thermal response of cosmetic compositions including NELUPURE

[0171] Various properties of cosmetic compositions containing NELUPURE as a component can be evaluated using the following 28-day protocol. Any composition mentioned in this disclosure can be evaluated using the following protocol, or any composition containing 2 wt% or 4 wt% NELUPURE can be analyzed using the following protocol.

[0172] According to the aforementioned protocol, participants washed their faces the night before the program began. On the first day of the program, participants did not wash their faces. Baseline measurements were established using one or more of the following: a sebum meter, sebufix, facial color skin quality analysis, and porphyrins. The product was applied, and measurements were taken at 1 hour, 4 hours, and 6 hours using one or more of the following: a sebum meter, sebufix, facial color skin quality analysis, and porphyrins. Participants then went to a sauna for 30 minutes. The product was applied twice daily for days 1–3.

[0173] On day 4, participants did not wash their faces the previous night. Baseline measurements were established using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Products were applied, and measurements were taken at 1 hour, 4 hours, and 6 hours using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Participants then went to a sauna for 30 minutes.

[0174] On day 14, participants did not wash their faces the previous night. Baseline measurements were established using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Products were applied, and measurements were taken at 1 hour, 4 hours, and 6 hours using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Participants then went to a sauna for 30 minutes.

[0175] On day 21, participants did not wash their faces the previous night. Baseline measurements were established using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Products were applied, and measurements were taken at 1 hour, 4 hours, and 6 hours using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Participants then went to a sauna for 30 minutes.

[0176] On day 28, participants did not wash their faces the previous night. Baseline measurements were established using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Products were applied, and measurements were taken at 1 hour, 4 hours, and 6 hours using one or more of the following: a sebometer, sebufix, facial color skin quality analysis, and porphyrins. Participants then went to a sauna for 30 minutes.

[0177] Example 9: Testing the sweat control and thermal response of cosmetic compositions including NELUPURE

[0178] In Example 9, two formulations were tested: a "placebo formulation" without any NELUPURE and a "prototype formulation" with NELUPURE. Both formulations used the setting spray formulations discussed in Examples 1-8 of this article.

[0179] An equivalent base facial serum formulation without Nelupure was used as a mediator control.

[0180] The various properties of the prototype formulation can be evaluated using the following method. Any composition mentioned in this disclosure can be evaluated using Nelupure at any concentration using the following method.

[0181] The study involved over 30 female participants with different skin types. After a conditioning period, technicians at the testing center applied a placebo and prototype formulation to the subjects once daily in a half-face control manner, and assessed their sweat production after a period of heat exposure in a sauna. Facial sweat was collected 50 and 100 minutes after heat exposure. This was performed on the first and fourth days, some time after the technicians applied the formulation.

[0182] Result. For example Figure 1 As shown, on day 1, the amount of facial sweat collected from skin treated with 2% Nelupure facial serum was 23% less than that collected from skin treated with 0% Nelupure facial serum, and on day 4 it was 41% less.

[0183] like Figure 2 As shown, on day 1, the amount of facial sweat collected from skin treated with 0.2% Nelupure facial serum was greater than that collected from skin treated with 0.2% Nelupure facial serum, and 45% less on day 4.

[0184] Example 10: Matte Composition

[0185] A matte composition comprising a low concentration of NELUPURE was prepared. The formulation is shown in Table 8 below.

[0186] Table 8

[0187]

[0188]

[0189] Example 11: Setting Spray

[0190] Setting sprays containing low concentrations of NELUPURE were prepared. Some included fragrances. Tables 9 and 10 below show the formulations.

[0191] Table 9

[0192]

[0193] Table 10

[0194]

[0195]

[0196] Example 12: Matte Composition

[0197] Table 11

[0198]

[0199] The terms and expressions used are descriptive and not restrictive, and their use is not intended to exclude any equivalents of the features shown and described or portions thereof, but it should be recognized that various modifications are possible within the scope of aspects of this disclosure. Therefore, it should be understood that while this disclosure is specifically disclosed by way of specific features, modifications and variations of the concepts disclosed herein can be taken by those skilled in the art, and such modifications and variations are considered to be within the scope of the embodiments described herein.

[0200] Exemplary aspect.

[0201] The following exemplary aspects are provided, and the numbering of these exemplary aspects should not be interpreted as indicating a level of importance:

[0202] Aspect 1. A cosmetic composition comprising: water in the range of about 55-65 wt%; aloe vera leaf juice in the range of about 0.050-0.150 wt%; sodium hyaluronate in the range of about 0.050-0.150 wt%; glycerin in the range of about 3.000-10.000 wt%; butylene glycol in the range of about 2.000-7.000 wt%; chlorphenesin in the range of about 0.0100-0.350 wt%; 3-O-ethyl ascorbic acid in the range of about 0.050-0.150 wt%; arginine in the range of about 0.035-0.090 wt%; PVP in the range of about 1.500-5.500 wt%; glycerin in the range of about 5.000-9.000 wt%; and sodium hyaluronate in the range of about 1.000-1.500 wt%. Acryloyl dimethyl taurate ammonium / behenyl ether-25 methacrylate crosspolymer in the range of about 0.500-1.500 wt%; 1,2-hexanediol and caprylyl glycol in the range of about 1.000-3.000 wt%; propylene glycol and glycerin and Chinese lotus extract and Egyptian blue water lily extract in the range of about 1.000-3.000 wt%; water and blue 1 in the range of about 0.100-0.300 wt%; and polysilicon-11 and water and lauryl ether-12 and ethylhexylglycerin in the range of about 8.000-12.000 wt%.

[0203] Aspect 2. A cosmetic composition comprising: water in the range of about 65,000-75,000 wt%; sodium hyaluronate in the range of about 0.0500-0.1500 wt%; glycerin in the range of about 10,000-18,000 wt%; ammonium acryloyl dimethyl taurate / behenol polyether-25 methacrylate crosspolymer in the range of about 0.150-0.250 wt%; cetearyl alcohol and cetearyl glucoside in the range of about 1,000-1.250 wt%; octyl dodecyl neopentanoate in the range of about 1,000-2,000 wt%; pentaerythritol tetraethylhexanoate in the range of about 1,000-3,000 wt%; butanediol in the range of about 2,000-7,000 wt%; and sodium hyaluronate in the range of about 0.050-0.110 wt%. Sodium polyacrylate starch in the range of about 0.250-0.750 wt%; dimethicone in the range of about 1.000-3.000 wt%; propylene glycol and glycerin and Chinese lotus extract and Egyptian blue water lily extract in the range of about 1.000-3.000 wt%; bisabolol in the range of about 0.050-0.150 wt%; water and cranberry fruit extract in the range of about 0.050-0.150 wt%; 1,2-hexanediol and caprylyl glycol in the range of about 0.050-1.500 wt%; and polysorbate 8 in the range of about 0.050-1.500 wt%.

[0204] Aspect 3. A cosmetic composition comprising: titanium dioxide and triethoxyoctanoyl silane in the range of about 1,000-3,000 wt%; tocopheryl acetate in the range of about 0.300-0.700 wt%; isododecane in the range of about 10,000-15,000 wt%; dioctyl carbonate and tocopherol in the range of about 2,000-5,000 wt%; dioctyl ether and tocopherol in the range of about 2,000-5,000 wt%; chromium oxide green in the range of about 0.500-1,000 wt%; iron oxide in the range of about 0.020-0.060 wt%; triethyl citrate in the range of about 0.700-1,100 wt%; water in the range of about 0.050-0.200 wt%; and a component in the range of about 0.100-0.300 wt%. Chlorphenesin in the range of wt%; silachlor bentonite in the range of about 1,000-3,000 wt%; a first functional mixture in the range of about 40,000-50,000 wt%; a second functional mixture in the range of about 10,000-15,000 wt%; 2,3-butanediol and water in the range of about 1,000-2,000 wt%; aluminum starch octenyl succinate in the range of about 1,000-5,000 wt%; silica in the range of about 4,000-8,000 wt%; propylene glycol and glycerin and lotus extract from Chinese water lily and water lily from Egyptian blue in the range of about 1,000-3,000 wt%; yeast ferment and lauroyl lysine in the range of about 0,400-1,000 wt%; and in the range of about 0.050-0.150 The first functional mixture comprises, in the range of wt%, glycerol and water and Centella asiatica leaf extract, wherein the first functional mixture comprises: water in the range of about 65,000-75,000 wt%; sodium chloride in the range of about 3,500-6,500 wt%; sodium benzoate in the range of about 0,200-0,600 wt%; dioctyl carbonate and tocopherol in the range of about 3,000-8,000 wt%; dioctyl ether and tocopherol in the range of about 3,000-8,000 wt%; tris(polyglycerol-3 / lauryl) hydrogenated trioleate in the range of about 1,000-5,000 wt%; and in the range of about 0,500-2,000 wt%. The second functional mixture comprises, in the range of wt%, cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydroxyhydrogenated cinnamate, wherein the second functional mixture comprises: dioctyl carbonate and tocopherol in the range of about 3,000-8,000 wt%; tris(polyglycerol-3 / lauryl) hydrogenated trimellioleate in the range of about 2,000-6,000 wt%; dioctyl ether and tocopherol in the range of about 3,500-6,500 wt%; isododecane in the range of about 6,000-9,000 wt%; and in the range of about 1.The composition includes: cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydroxyhydrocinnamate in the range of about 0.000-5.000 wt%; trimethylsiloxysilicate in the range of about 13.000-19.000 wt%; silica in the range of about 2.000-6.000 wt%; dimethylsilyl silica in the range of about 1.000-4.000 wt%; water in the range of about 30.000-39.000 wt%; glycerol in the range of about 8.000-12.000 wt%; sodium chloride in the range of about 2.000-6.000 wt%; and sodium benzoate in the range of about 0.200-0.600 wt%.

[0205] Aspect 4. A cosmetic composition comprising: dioctyl carbonate and tocopherol in the range of about 2,000-6,000 wt%; dioctyl ether and tocopherol in the range of about 2,000-6,000 wt%; cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydroxyhydrogenated cinnamate in the range of about 0.500-4,0000 wt%; iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated linoleate and cetyl PEG / PPG 10 / 1 in the range of about 0.500-2,300 wt%; and iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated linoleate and cetyl PEG / PPG in the range of about 0.300-0.900 wt%. 10 / 1; iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated trioleate and cetyl PEG / PPG 10 / 1 in the range of about 0.100-0.600 wt%; titanium dioxide and triethoxyoctanoyl silane in the range of about 6.000-10.000 wt%; zinc oxide [nano] and triethoxyoctanoyl silane in the range of about 2.000-8.000 wt%; silicon dioxide in the range of about 2.000-6.000 wt%; isododecane in the range of about 2.00-6.000 wt%; aluminum starch octenyl succinate in the range of about 2.00-6.000 wt%; silachlor bentonite in the range of about 0.500-2.000 wt%; in the range of about 0.500-1.500 The third functional mixture in the range of about 10,000-15,000 wt%; the second functional mixture in the range of about 32,000-39,000 wt%; the first functional mixture in the range of about 0.500-3.500 wt%; 2,3-butanediol and water in the range of about 0.100-0.400 wt%; glycerol in the range of about 1,000-3.500 wt%; α-glucan oligosaccharides in the range of about 0.150-0.350 wt%; sodium hyaluronate in the range of about 0.050-0.200 wt%; dimethyl methoxybenzodihydropyranol in the range of about 0.002-0.008 wt%; vitamin E acetate in the range of about 0.300-0.800 wt%; and 2,3-butanediol and water in the range of about 0.300-0.800 wt%. Caprylic / capric triglycerides and Rosa Damascus flower extract in the range of about 0.500-3.000 wt%; nicotinamide in the range of about 0.500-3.000 wt%; and propylene glycol and glycerin and lotus extract from China and water lily from Egypt in the range of about 0.500-3.000 wt%, wherein the first functional mixture comprises: water in the range of about 65.000-75.000 wt%; and in the range of about 3.The second functional mixture comprises: sodium chloride in the range of about 500-6.500 wt%; sodium benzoate in the range of about 0.200-0.600 wt%; dioctyl carbonate and tocopherol in the range of about 3.000-8.000 wt%; dioctyl ether and tocopherol in the range of about 3.000-8.000 wt%; tri(polyglycerol-3 / lauryl) hydrogenated trioleate in the range of about 1.000-5.000 wt%; cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydroxyhydrogenated cinnamate in the range of about 0.500-2.000 wt%, wherein the second functional mixture comprises: dioctyl carbonate and tocopherol in the range of about 3.000-8.000 wt%; dioctyl ether and tocopherol in the range of about 2.000-6.000 wt%. The composition includes: tri(polyglycerol-3 / lauryl) hydrogenated trioleate in the range of about 3,500-6,500 wt%; dioctyl ether and tocopherol in the range of about 6,000-9,000 wt%; isododecane in the range of about 1,000-5,000 wt%; cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydroxyhydrogenated cinnamate in the range of about 13,000-19,000 wt%; trimethylsiloxysilicate in the range of about 2,000-6,000 wt%; silica in the range of about 1,000-4,000 wt%; dimethylsilyl alkylated silica in the range of about 30,000-39,000 wt%; and water in the range of about 8,000-12,000 wt%. The mixture comprises: glycerol in the range of about 2,000-6,000 wt%; sodium chloride in the range of about 0,200-0,600 wt%; and sodium benzoate in the range of about 0,200-0,600 wt%, wherein the third functional mixture comprises: propylene carbonate in the range of about 60-75 wt%; chlorphenesin in the range of about 15-25 wt%; and purified water conforming to USP, PhEur, JP, and JSCI in the range of about 5-15 wt%.

[0206] Aspect 5. A method for reducing facial sweat production in human skin, the method comprising the steps of: treating skin requiring sweat reduction with a sweat-reducing cosmetic composition at least once daily at a sufficient concentration and duration, thereby reducing facial sweat compared to skin treated with the same cosmetic composition as the sweat-reducing cosmetic composition except for the absence of a sweat-reducing agent, as evaluated by the method described in Example 8 herein, wherein the sweat-reducing cosmetic composition contains an effective amount of the sweat-reducing active agent for skin in a physiologically compatible medium.

[0207] Aspect 6. The method according to aspect 5, wherein the cosmetic composition for reducing sweat does not contain pigments or colorants.

[0208] Aspect 7. The method according to aspect 5, wherein when measured at the end of day 1 and day 4 of the treatment, the treatment resulted in a reduction of facial perspiration by at least 25%.

[0209] Aspect 8. The method according to aspect 5, wherein when measured at the end of the fourth day of treatment, the treatment resulted in a reduction of facial perspiration by at least 25%.

[0210] Aspect 9. The method according to aspect 5, wherein the concentration of the sweat-reducing active agent contained therein is at least 2% of the cosmetic composition.

[0211] Aspect 10. The method according to aspect 5, wherein the concentration of the sweat-reducing active agent contained therein is at least 0.2% and less than 2% of the cosmetic composition.

[0212] Aspect 11. The method according to aspect 10, wherein the cosmetic composition for reducing sweat comprises a variety of formulations.

[0213] Aspect 12. The method according to aspect 11, wherein the first formulation is intended to be applied on days 1-4 of treatment, and the second and subsequent formulations are intended to be applied on day 5 and thereafter of treatment.

[0214] Aspect 13. The method according to aspect 12, wherein the first formulation comprises at least 0.2% NELUPURE and a second sweat absorber and / or sweat reducer, and the second formulation comprises at least 0.2% NELUPURE, and wherein the second formulation optionally contains the second sweat absorber and / or sweat reducer.

Claims

1. A cosmetic composition comprising: Water in the range of approximately 55-65 wt%; Aloe Barbadensis leaf juice in the range of approximately 0.050-0.150 wt%; Sodium hyaluronate in the range of approximately 0.050-0.150 wt%; Glycerin in the range of approximately 3,000–10,000 wt%; Butanediol in the range of approximately 2,000–7,000 wt%; Chlorphenesin in the range of approximately 0.0100-0.350 wt%; 3-O-ethyl ascorbic acid in the range of approximately 0.050-0.150 wt%; Arginine in the range of approximately 0.035–0.090 wt%; PVP in the range of approximately 1,500–5,500 wt%; Glycerin in the range of approximately 5,000–9,000 wt%; Acryloyl dimethyl taurate ammonium / behenol polyether-25 methacrylate crosspolymer in the range of about 1,000-1,500 wt%; 1,2-hexanediol and octyl glycol in the range of approximately 0.500-1.500 wt%; Propylene glycol and glycerin in the range of approximately 1,000–3,000 wt%, and extracts of Chinese lotus (Nelumbo Nucifera) flower and Egyptian blue water lily (Nymphaea Caerulea) flower; Water and Blue 1 in the range of approximately 0.100–0.300 wt%; and Polysilicone-11 and water and lauryl ether-12 and ethylhexylglycerin in the range of about 8,000-12,000.

2. A cosmetic composition comprising: Water in the range of approximately 65,000–75,000 wt%; Sodium hyaluronate in the range of approximately 0.0500-0.1500 wt%; Glycerin in the range of approximately 10,000–18,000 wt%; Acryloyl dimethyl taurate ammonium / behenol polyether-25 methacrylate crosspolymer in the range of about 0.150-0.250 wt%; Cetearyl alcohol and cetearyl glucoside in the range of about 1,000-1,250 wt%; Octyl dodecyl neopentanoate in the range of approximately 1,000–2,000 wt%; Pentaerythritol tetraethylhexanoate in the range of approximately 1,000–3,000 wt%; Butanediol in the range of approximately 2,000–7,000 wt%; Sodium polyacrylate starch in the range of approximately 0.050-0.110 wt%; Dimethicone in the range of approximately 0.250-0.750 wt%; Propylene glycol and glycerin, and Chinese lotus extract and Egyptian blue water lily extract, in the range of approximately 1,000-3,000 wt%; Bisabolol in the range of approximately 0.050–0.150 wt%; Water and Vaccinium macrocarpon (cranberry) fruit extract in the range of about 0.050-0.150 wt%; 1,2-hexanediol and caprylyl glycol; 1,2-hexanediol and octaneglycol in the range of about 0.050-1,500 wt%; and Polysorbate 8 in the range of approximately 0.050-1.500 wt%.

3. A cosmetic composition comprising: Titanium dioxide and triethoxyoctanoylsilane in the range of approximately 1,000–3,000 wt%; Tocopheryl acetate in the range of approximately 0.300–0.700 wt%; Isododecane in the range of approximately 10,000–15,000 wt%; Dioctyl carbonate and tocopherol in the range of approximately 2,000–5,000 wt%; Dioctyl ether and tocopherol in the range of approximately 2,000–5,000 wt%; Chromium oxide green in the range of approximately 0.500-1.000 wt%; Iron oxide in the range of approximately 0.020–0.060 wt%; Triethyl citrate in the range of approximately 0.700–1.100 wt%; Water in the range of approximately 0.050–0.200 wt%; Chlorphenesin in the range of approximately 0.100-0.300 wt%; Siladiamine bentonite in the range of approximately 1,000–3,000 wt%; The first functional mixture is in the range of approximately 40,000-50,000 wt%. A second functional mixture in the range of approximately 10,000-15,000 wt%; 2,3-Butanediol and water in the range of approximately 1,000-2,000 wt%; Aluminum starch octenyl succinate in the range of approximately 1,000–5,000 wt%; Silica in the range of approximately 4,000–8,000 wt%; Propylene glycol and glycerin, and Chinese lotus extract and Egyptian blue water lily extract, in the range of approximately 1,000-3,000 wt%; Saccharomyces ferment and lauroyl lysine in the range of approximately 0.400–1.000 wt%; and Glycerin and water and Centella Asiatica leaf extract in the range of approximately 0.050-0.150 wt%. The first functional mixture comprises: Water in the range of approximately 65,000–75,000 wt%; Sodium chloride in the range of approximately 3,500–6,500 wt%; Sodium benzoate in the range of approximately 0.200-0.600 wt%; Dioctyl carbonate and tocopherol in the range of approximately 3,000–8,000 wt%; Dioctyl ether and tocopherol in the range of approximately 3,000–8,000 wt%; Tris(polyglycerol-3 / lauryl) hydrogenated trimer linoleate in the range of approximately 1,000–5,000 wt%; Cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydrocinnamate, in the range of approximately 0.500-2.000 wt%, The second functional mixture comprises: Dioctyl carbonate and tocopherol in the range of approximately 3,000–8,000 wt%; Tris(polyglycerol-3 / lauryl) hydrogenated trimer linoleate in the range of approximately 2,000–6,000 wt%; Dioctyl ether and tocopherol in the range of approximately 3,500–6,500 wt%; Isododecane in the range of approximately 6,000–9,000 wt%; Cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butyl hydroxyhydrocinnamate in the range of approximately 1,000-5,000 wt%; Trimethylsilyloxysilicate in the range of approximately 13,000–19,000 wt%; Silica in the range of approximately 2,000–6,000 wt%; Dimethylsilylated silica in the range of approximately 1,000–4,000 wt%; Water in the range of approximately 30,000–39,000 wt%; Glycerin in the range of approximately 8,000–12,000 wt%; Sodium chloride in the range of approximately 2,000–6,000 wt%; and Sodium benzoate in the range of approximately 0.200-0.600 wt%.

4. A cosmetic composition comprising: Dioctyl carbonate and tocopherol in the range of approximately 2,000–6,000 wt%; Dioctyl ether and tocopherol in the range of approximately 2,000–6,000 wt%; Cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butyl hydroxyhydrocinnamate in the range of approximately 0.500-4.0000 wt%; Iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated trioleate and cetyl PEG / PPG 10 / 1 in the range of about 0.500-2.300 wt%; Iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated trioleate and cetyl PEG / PPG 10 / 1 in the range of about 0.300-0.900 wt%; Iron oxide and dioctyl carbonate and dioctyl ether and tris(polyglycerol-3 / lauryl) hydrogenated trioleate and cetyl PEG / PPG 10 / 1 in the range of about 0.100-0.600 wt%; Titanium dioxide and triethoxyoctanoylsilane in the range of approximately 6,000-10,000 wt%; Zinc oxide [nano] and triethoxyoctanoylsilane in the range of approximately 2,000-8,000 wt%; Silica in the range of approximately 2,000–6,000 wt%; Isododecane in the range of approximately 2,000–6,000 wt%; Aluminum starch octenyl succinate in the range of approximately 2.00–6.000 wt%; Siladiamine bentonite in the range of approximately 0.500-2.000 wt%; A third functional mixture in the range of approximately 0.500–1.500 wt%; A second functional mixture in the range of approximately 10,000-15,000 wt%; The first functional mixture is in the range of approximately 32,000-39.00 wt%. 2,3-Butanediol and water in the range of approximately 0.500-3.500 wt%; Fragrances in the range of approximately 0.100-0.400 wt%; Glycerin in the range of approximately 1,000–3,500 wt%; α-glucan oligosaccharides in the range of approximately 0.150–0.350 wt%; Sodium hyaluronate in the range of approximately 0.050-0.200 wt%; Dimethylmethoxybenzodihydropyranol in the range of about 0.002-0.008 wt%; Vitamin E acetate in the range of approximately 0.300-0.800 wt%; Caprylic / capric triglycerides and Damask rose (Centella Asiatica) flower extract, in the range of approximately 0.300–0.800 wt%. Nicotinamide in the range of approximately 0.500–3.000 wt%; and Propylene glycol and glycerin, along with Chinese lotus extract and Egyptian blue water lily extract, in the range of approximately 0.500–3.000 wt%. The first functional mixture comprises: Water in the range of approximately 65,000–75,000 wt%; Sodium chloride in the range of approximately 3,500–6,500 wt%; Sodium benzoate in the range of approximately 0.200-0.600 wt%; Dioctyl carbonate and tocopherol in the range of approximately 3,000–8,000 wt%; Dioctyl ether and tocopherol in the range of approximately 3,000–8,000 wt%; Tris(polyglycerol-3 / lauryl) hydrogenated trimer linoleate in the range of approximately 1,000–5,000 wt%; Cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butylhydrocinnamate, in the range of approximately 0.500-2.000 wt%, The second functional mixture comprises: Dioctyl carbonate and tocopherol in the range of approximately 3,000–8,000 wt%; Tris(polyglycerol-3 / lauryl) hydrogenated trimer linoleate in the range of approximately 2,000–6,000 wt%; Dioctyl ether and tocopherol in the range of approximately 3,500–6,500 wt%; Isododecane in the range of approximately 6,000–9,000 wt%; Cetyl PEG / PPG-10 / 1 dimethicone and pentaerythritol tetra-di-tert-butyl hydroxyhydrocinnamate in the range of approximately 1,000-5,000 wt%; Trimethylsilyloxysilicate in the range of approximately 13,000–19,000 wt%; Silica in the range of approximately 2,000–6,000 wt%; Dimethylsilylated silica in the range of approximately 1,000–4,000 wt%; Water in the range of approximately 30,000–39,000 wt%; Glycerin in the range of approximately 8,000–12,000 wt%; Sodium chloride in the range of approximately 2,000–6,000 wt%; and Sodium benzoate in the range of approximately 0.200-0.600 wt%, The third functional mixture comprises: Propylene carbonate in the range of approximately 60-75 wt%; Chlorphenesin in the range of approximately 15-25 wt%; and Purified water conforming to USP, PhEur, JP, and JSCI standards, ranging from approximately 5-15 wt%.

5. A method for reducing facial sweat production in human skin, the method comprising the following steps: Skin requiring reduced sweating is treated at least once daily with a sufficient concentration and duration using a sweat-reducing cosmetic composition, thereby reducing facial sweating compared to skin treated with the same cosmetic composition as the sweat-reducing cosmetic composition except for the absence of a sweat-reducing active agent, as evaluated by the method described in Example 9 herein, wherein the sweat-reducing cosmetic composition contains an effective amount of the sweat-reducing active agent for the skin in a physiologically compatible medium.

6. The method of claim 5, wherein the cosmetic composition for reducing sweat does not contain pigments or colorants.

7. The method of claim 5, wherein when measured at the end of the fourth day of treatment, the treatment resulted in a reduction of facial sweat by at least 25%.

8. The method of claim 5, wherein when measured at the end of the fourth day of treatment, the treatment resulted in a reduction of facial sweat by at least 25%.

9. The method of claim 5, wherein the concentration of the sweat-reducing active agent contained therein is at least 2% of the cosmetic composition.

10. The method of claim 5, wherein the concentration of the sweat-reducing active agent contained therein is at least 0.2% and less than 2% of the cosmetic composition.

11. The method of claim 10, wherein the cosmetic composition for reducing sweat comprises a variety of formulations.

12. The method of claim 11, wherein the first formulation is intended to be applied on days 1-4 of the treatment, and the second and subsequent formulations are intended to be applied on day 5 and thereafter of the treatment.

13. The method of claim 12, wherein the first formulation comprises at least 0.2% NELUPURE and a second absorbent and / or sweat-reducing agent, and the second formulation comprises at least 0.2% NELUPURE, and wherein the second formulation optionally contains the second absorbent and / or sweat-reducing agent.

14. The method of claim 5, wherein the method described in Example 9 comprises: The formula was applied to the skin of multiple participants; The skin is exposed to a predetermined amount of heat in a sauna for a predetermined time; and Evaluate the sweat production on the skin to which the formulation is applied.

15. The method of claim 14, wherein the formulation comprises a prototype formulation, a placebo formulation, or both.

16. The method of claim 14, wherein the predetermined time is 50 minutes.

17. The method of claim 14, wherein the predetermined time is 100 minutes.

18. The method of claim 14, wherein sweat production is evaluated once daily.

19. The method of claim 18, wherein the evaluation of sweat production is performed over four days.

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