Zinc finger protein zfp genes and uses thereof

By targeting and knocking out the zinc finger protein ZFP gene, and using the CRISPR/Cas9 system to knock out the ZFP gene in silkworms, combined with the PTEN promoter site, the problem that BmPGRP2 does not directly regulate PTEN was solved, and the antiviral ability of silkworms was significantly improved.

CN122629073APending Publication Date: 2026-08-25GERMPLASM INNOVATION GRAND SCIENCE CENTER OF WESTERN CHINA (CHONGQING) SCIENCE CITY
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Patent Information

Application Number
CN202611127597.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-07-28
Publication Date
2026-08-25

AI Technical Summary

Technical Problem

In existing technologies, the main methods for preventing and controlling silkworm nucleopolyhedrovirus (BmNPV) are focused on the target gene BmPGRP2. However, BmPGRP2 does not directly regulate PTEN, resulting in insufficient antiviral ability. Therefore, it is urgent to study its signal transduction molecules to improve the antiviral ability of silkworms.

Method used

By targeting and knocking out the zinc finger protein ZFP gene (ZFP), the ZFP gene was knocked out in silkworms using the CRISPR/Cas9 system. Transgenic individuals were then screened, and the ZFP gene was combined with a specific site GACATAGATATT on the PTEN promoter to inhibit PTEN transcription, thereby enhancing the antiviral ability of silkworms.

Benefits of technology

It significantly upregulated PTEN expression, inhibited BmNPV proliferation, and enhanced the antiviral ability of silkworms, providing an effective molecular breeding method to enhance the antiviral resistance of silkworms.

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Abstract

The application discloses a zinc finger protein ZFP gene and application thereof, and identifies the interaction protein ZFP of BmPGRP2 through yeast two-hybrid screening; the ZFP directly combines with the GACATAGATATT sequence on the PTEN promoter as a transcription factor, and inhibits the transcription of PTEN; the expression amount of PTEN can be significantly increased by knocking out the ZFP, and the proliferation of BmNPV is inhibited. Therefore, the ZFP is a negative regulation factor of PTEN, has important value in the research and application of the silkworm antiviral molecular breeding, and the antiviral ability of the silkworm can be improved by knocking out the gene through gene editing.
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