Screening device for cell separation

Abstract

The utility model discloses a screening device for cell separation. The screening device comprises a shell and a wicking assembly arranged above the shell, an automatic dilution system, a supporting table and a valve are arranged on the surface of the shell, a viscosity monitoring system, a temperature control system and a stirrer are arranged in the shell, and a wicking assembly connected with the supporting table through a telescopic assembly comprises a screening plate and a wicking bundle; the wicking beam adsorbs, separates and screens the cells by virtue of a wicking effect, a capillary action and an adhesion characteristic of the cells to the wicking beam, a stirrer is used for generating eddy current to stir liquid, and the whole cell adsorption screening and separation filtering process does not have too much mechanical friction, so that the problem of physical damage caused by cell separation is solved; the temperature control system can maintain a constant-temperature environment through a temperature sensor and a heating rod, the problem that the cell activity is reduced is solved, after adsorption screening, the shell can be subjected to separation and filtration operation after being cleaned, and the device is more flexible.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a screening device for cell separation. Background Art

[0002] In cell biology research, it is often necessary to separate specific cell types from complex cell samples. The use of screening devices can greatly improve the efficiency and accuracy of cell separation and screening.

[0003] A Chinese patent with authorization announcement number CN219586082U discloses a lymphocyte filtration and screening device. The device comprises a separation chamber and a filtration chamber connected to each other, a bracket for mounting a nylon wool column in the separation chamber, and a stirring of the cell sample in the chamber and the adsorption of the nylon wool column to achieve cell screening. The screened cells are then placed in the filtration chamber for filtration and flushing, thereby completing the filtration and screening of the cells. However, the device has relatively high mechanical friction during the stirring and filtration process, which can easily cause physical damage to the cells. Furthermore, the device does not take into account constant temperature control. The standard temperature for human cell culture is 36.5°C ± 0.5°C. Deviating from this temperature range will affect the normal metabolism of cells, thereby affecting cell activity and even causing cell death. In addition, the filtration chamber of the device can only be equipped with one filter plate, and it is not possible to filter and collect cells of multiple sizes at the same time.

[0004] Therefore, a new technical solution is needed to solve the existing technical problems. Summary of the Invention

[0005] The utility model overcomes the deficiencies of the prior art and provides a screening device for cell separation.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a screening device for cell separation, comprising: a shell, and a wicking component arranged above the shell.

[0007] The shell is a topless box, one side surface of the shell is connected to an automatic dilution system, and a temperature control system and a viscosity monitoring system are arranged inside the shell.

[0008] The wicking assembly includes: a support platform externally connected to the surface of one side of the shell and located above the shell, a telescopic assembly with one end vertically connected to the support platform, a screening plate connected to the other end of the telescopic assembly, and a plurality of wicking beams movably connected to the screening plate and vertically downward.

[0009] The size of the screening plate is no larger than the top opening of the shell.

[0010] In a preferred embodiment of the present invention, the automatic dilution system includes: a plurality of peristaltic pumps that can accurately extract liquid; a plurality of flow sensors connected to the plurality of peristaltic pumps that can monitor the amount of extracted liquid; and an agitator embedded in the bottom plate that can generate vortexes to stir the liquid.

[0011] In a preferred embodiment of the present invention, the temperature control system includes: a temperature sensor arranged at the bottom of the shell, used to monitor the temperature of the liquid in the shell; and a heating rod arranged at the bottom of the shell, used to heat the liquid in the shell.

[0012] In a preferred embodiment of the present invention, the viscosity monitoring system includes: a viscosity sensor disposed at the bottom of the housing, for monitoring the viscosity of the liquid in the housing.

[0013] In a preferred embodiment of the present invention, the telescopic assembly includes: a sleeve rod connected to the support platform, an extension rod arranged inside the sleeve rod; and a driving mechanism arranged inside the sleeve rod and connected to one end of the extension rod, which can drive the extension rod to move axially along the direction of the sleeve rod; the driving mechanism is a linear motor or a cylinder.

[0014] In a preferred embodiment of the present invention, a control system is provided on the surface of the shell, and the control system is respectively connected to the automatic dilution system, the viscosity monitoring system, the temperature control system and the driving mechanism, so as to achieve more convenient and accurate control and operation.

[0015] In a preferred embodiment of the present invention, the bottom of the shell is configured as a bottom plate with an inclination angle of 15°-60°, which can facilitate the liquid to flow to one side and reduce the residual liquid in the shell.

[0016] In a preferred embodiment of the present invention, the plurality of wicking bundles are a plurality of nylon wool columns composed of nylon fibers with a diameter of 10-80 μm.

[0017] In a preferred embodiment of the present invention, a plurality of the wicking bundles are equidistantly arranged below the screening plate, and the diameters of the plurality of the wicking bundles are 0.5-10 cm and the lengths are 5-20 cm.

[0018] In a preferred embodiment of the present invention, a plurality of valves are provided at the bottom of one side of the shell.

[0019] The present invention solves the defects in the background technology and has the following beneficial effects:

[0020] (1) The present invention provides a screening device for cell separation. When separating and screening cells, the wicking beam is precisely controlled to contact the liquid surface and immersion depth of the cell sample. The wicking beam relies on capillary action and wicking effect, as well as the adhesion characteristics of the cells to the wicking beam to adsorb cells, thereby achieving the separation of cells of different sizes and masses. The wicking beam is then completely immersed in a new diluent, and a vortex is generated by a stirrer to stir the liquid, thereby accelerating the detachment of cells adsorbed on the wicking beam. The liquid is then filtered and collected through a valve to obtain the adsorbed cell liquid. The entire cell separation and screening process is relatively gentle, without excessive mechanical friction, and solves the problem of physical damage to cells caused by the screening process.

[0021] (2) The present invention provides a screening device for cell separation, which relies on the wicking effect, capillary action, and the adhesion characteristics of cells to the wicking beam to adsorb and separate cells. The capillary force is related to the adhesion between the liquid and the solid (such as surface energy) and the cohesive force of the liquid itself (determined by viscosity). When the viscosity of the cell sample increases, the cohesive force of the liquid also increases, which will weaken the capillary force, causing the adsorbed liquid to rise more slowly or unable to rise. Through the coordinated work of the peristaltic pump and the flow sensor, the flow rate of the diluent can be accurately controlled, and then the viscosity sensor can be used to monitor to ensure that the cell sample reaches the optimal dilution ratio, thereby solving the problem of insufficient dilution of the cell sample and high viscosity leading to poor adsorption and separation effect.

[0022] (3) The utility model provides a screening device for cell separation. During the entire process of separating and screening cells, the control system is connected to the temperature sensor and controls the heating rod. The heating rod heats the liquid in the shell by heat conduction and heat convection, thereby controlling the liquid in the shell to be within a set constant temperature range, thereby solving the problem of decreased cell activity or even inactivation.

[0023] (4) The present invention provides a screening device for cell separation. After the adsorption screening process is completed, a diluent is transported into the shell through a peristaltic pump, and then a vortex is generated by the rotation of the agitator to clean the inside of the shell. The cleaning waste liquid is then discharged through a valve. The cleaning operation is repeated many times until the inside of the shell is clean, and then a separation and filtration process for screening cells is performed. In this way, the shell serves as an adsorption screening chamber and, after cleaning, as a separation and filtration chamber. Both processes are carried out in one shell, thereby reducing the footprint of the device.

[0024] (5) The present invention provides a screening device for cell separation. When filtering the cell liquid after adsorption screening, screens of different specifications are installed at several valves, thereby being able to filter and collect cells of multiple different sizes at the same time, making the device more flexible.

[0025] (6) The utility model provides a screening device for cell separation. The inclined bottom plate design facilitates the liquid to flow to one side, reduces the liquid residue in the shell, facilitates subsequent operation and collection, and thus improves the efficiency of separating and screening cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The present invention is further described below with reference to the accompanying drawings and embodiments;

[0027] Figure 1 It is a three-dimensional structural diagram of a preferred embodiment of the utility model;

[0028] Figure 2 It is a front cross-sectional view of a preferred embodiment of the utility model;

[0029] In the figure: 1. Shell; 2. Support platform; 3. Bottom plate; 4. Screening plate; 5. Wicking beam; 6. Automatic dilution system; 7. Viscosity sensor; 8. Temperature sensor; 9. Heating rod; 10. Agitator; 11. Sleeve rod; 12. Extension rod; 13. Valve. DETAILED DESCRIPTION

[0030] The present invention will now be further described in detail with reference to the accompanying drawings and embodiments, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the devices or elements referred to must have a specific direction, be constructed and operate in a specific direction, and therefore should not be understood as limiting the present invention. In the present invention, unless otherwise expressly specified or limited, the terms "disposed", "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, they can refer to fixed connections or detachable connections; mechanical connections; direct connections or indirect connections through an intermediate medium. For those skilled in the art, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0031] These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0032] like Figure 1 and Figure 2 As shown, a screening device for cell separation includes: a shell 1, and a wicking component arranged above the shell 1.

[0033] The shell 1 is a topless box, and the shell 1 includes: an automatic dilution system 6 connected to the surface of one side of the shell 1, and a viscosity monitoring system and a temperature control system arranged inside the shell 1. The automatic dilution system 6 includes: a number of peristaltic pumps, a number of flow sensors connected to the peristaltic pumps, and an agitator 10 embedded in the bottom plate 3. The viscosity monitoring system includes: a viscosity sensor 7 arranged at the bottom of the shell 1. The temperature control system includes: a temperature sensor 8 arranged at the bottom of the shell 1, and a heating rod 9 arranged at the bottom of the shell 1. The bottom of the shell 1 is set as a bottom plate 3 with an inclination angle of 15 to 60 degrees. A number of valves 13 are set at the bottom of one side of the shell 1.

[0034] The wicking assembly includes: a support platform 2 externally connected to the surface of one side of the shell 1 and located above the shell 1, a telescopic assembly with one end vertically connected to the support platform 2, a screening plate 4 connected to the other end of the telescopic assembly, and a plurality of wicking bundles 5 movably connected to the screening plate 4 and vertically downward. The telescopic assembly includes: a sleeve rod 11 connected to the support platform 2, an extension rod 12 arranged inside the sleeve rod 11; and a driving mechanism arranged inside the sleeve rod 11 and connected to one end of the extension rod 12; the driving mechanism is a linear motor or a cylinder. The size of the screening plate 4 is no larger than the top opening of the shell 1. The plurality of wicking bundles 5 are a plurality of nylon wool columns composed of nylon fibers with a diameter of 10-80μm. The plurality of wicking bundles 5 are equidistantly arranged below the screening plate 4. The diameter of the plurality of wicking bundles 5 is 0.5-10cm and the length is 5-20cm.

[0035] A control system is provided on the surface of the housing 1 , and the control system is respectively connected to the automatic dilution system 6 , the viscosity monitoring system, the temperature control system and the driving mechanism.

[0036] When the present invention is used, a certain amount of T and B lymphocyte samples are accurately extracted by the peristaltic pump in the automatic dilution system 6 and transported into the housing 1, and then another peristaltic pump accurately extracts physiological saline to dilute the cell sample. At the same time, the stirrer 10 is turned on, and the stirrer 10 accelerates the mixing of the liquid by generating vortices through rotation. The temperature control system is turned on, and the temperature sensor 8 monitors the temperature of the liquid in the housing 1, and cooperates with the control heating rod 9 to maintain the temperature of the liquid in the housing 1 at 36.5°C ± 0.5°C. The viscosity sensor 7 then detects the viscosity of the T and B lymphocyte samples in the housing 1, and stops transporting physiological saline when the viscosity reaches the set threshold. The drive mechanism is started, and the telescopic component begins to extend, driving the screening plate 4 connected to the wicking beam 5 to move downward. The control system accurately controls the drive mechanism, thereby accurately controlling the depth of the wicking beam 5 in contact with the liquid surface and immersed in the liquid. The wicking beam 5 relies on capillary action and wicking effect, as well as the adhesion characteristics of B lymphocytes to the wicking beam to adsorb B lymphocytes, thereby achieving separation of T lymphocytes and B lymphocytes. After adsorption and separation are completed, the drive device is controlled to retract the telescopic assembly, causing the wicking beam 5 to leave the liquid surface and return to the top of the housing 1. Valve 13 is opened to discharge the enriched T lymphocyte suspension within the housing 1. The angled bottom plate 3 facilitates liquid drainage and reduces liquid residue. Valve 13 is then closed. A peristaltic pump is then used to deliver physiological saline into the housing 1. The agitator 10 rotates to generate a vortex to clean the interior of the housing 1. Valve 13 is then opened to discharge the waste cleaning liquid. This cleaning operation can be repeated multiple times until the interior of the housing 1 is completely clean. Close the valve 13, deliver physiological saline into the shell 1 through the peristaltic pump, turn on the temperature control system, and then start the driving mechanism. The telescopic component extends to drive the screening plate 4 connected to the wicking bundle 5 to move downward. The control system accurately controls the driving mechanism to control the wicking bundle 5 to be completely immersed in the physiological saline. The agitator 10 generates a vortex to stir the liquid, which accelerates the detachment of the B lymphocytes adsorbed on the wicking bundle 5. The driving mechanism is controlled to contract the telescopic component to make the wicking bundle 5 leave the liquid faster, and the adsorbed and screened B lymphocyte suspension is obtained. If needed, screens of different specifications and sizes can be installed at the outlet of the valve 13. Then, the valve 13 is opened for filtration and the enriched B lymphocyte suspension is collected.

[0037] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0038] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art. Relevant personnel can make various changes and modifications without departing from the scope of the technical idea of this utility model.

Claims

1. A screening device for cell separation, comprising: A shell (1), and a wicking assembly arranged above the shell (1), characterized in that: The housing (1) is a topless box; one side surface of the housing (1) is connected to an automatic dilution system (6); a temperature control system and a viscosity monitoring system are provided inside the housing (1); the bottom of the housing (1) is provided as a bottom plate (3) with an inclination angle of 15°-60°; The wicking assembly comprises: a support platform (2) externally connected to a surface of one side of the shell (1) and located above the shell (1), a telescopic assembly with one end vertically connected to the support platform (2), a screening plate (4) connected to the other end of the telescopic assembly, and a plurality of wicking beams (5) connected to the screening plate (4) and extending vertically downward; The size of the screening plate (4) is no larger than the top opening of the shell (1).

2. A cell separation screening device according to claim 1, characterized in that: The automatic dilution system (6) includes: a plurality of peristaltic pumps, a plurality of flow sensors connected to the peristaltic pumps, and a stirrer (10) embedded in the bottom plate (3).

3. The cell separation screening device according to claim 1, characterized in that: The temperature control system comprises: a temperature sensor (8) arranged at the bottom inner of the shell (1), and a heating rod (9) arranged at the bottom inner of the shell (1).

4. The cell separation screening device according to claim 1, wherein: The viscosity monitoring system comprises a viscosity sensor (7) arranged at the bottom of the housing (1).

5. The cell separation screening device according to claim 1, characterized in that: The telescopic assembly comprises: a sleeve rod (11) connected to the support platform (2), an extension rod (12) arranged inside the sleeve rod (11); and a driving mechanism arranged inside the sleeve rod (11) and connected to one end of the extension rod (12); the driving mechanism is a linear motor or a cylinder.

6. The cell separation screening device according to claim 5, characterized in that: A control system is provided on the surface of the housing (1), and the control system is respectively connected to the automatic dilution system (6), the viscosity monitoring system, the temperature control system and the driving mechanism.

7. The cell separation screening device according to claim 1, characterized in that: The plurality of wicking bundles (5) are a plurality of nylon wool columns composed of nylon fibers with a diameter of 10-80 μm.

8. The cell separation screening device according to claim 1, characterized in that: The plurality of wicking bundles (5) are arranged equidistantly below the screening plate (4), and the diameters of the plurality of wicking bundles (5) are 0.5-10 cm and the lengths are 5-20 cm.

9. The cell separation screening device according to claim 1, characterized in that: A plurality of valves (13) are provided at the bottom of one side of the housing (1).

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