Tremella fuciformis liquid inoculum device

Abstract

This utility model relates to a liquid cultivar inoculation device for Tremella fuciformis, comprising a conveying device for conveying a tray containing culture bottles and an inoculation mechanism located above the conveying device. The inoculation mechanism includes an inoculation plate frame located above the conveying device and capable of being raised and lowered. The inoculation plate frame is equipped with inoculation tubes corresponding one-to-one with the culture bottles on the tray. An inoculation control device for conveying Tremella fuciformis liquid cultivar spawn to the inoculation tubes is located above the inoculation plate frame. This Tremella fuciformis liquid cultivar inoculation machine is convenient to use and highly practical, realizing mechanized inoculation of Tremella fuciformis liquid cultivar spawn. It not only improves production efficiency, reduces labor intensity, saves manpower, and reduces the number of personnel entering the sterile room, thus reducing the risk of cultivar contamination, but also allows for stable control of the inoculation amount, improving the accuracy of the inoculation amount, making the inoculation amount more uniform, improving the inoculation quality, and promoting subsequent mycelial growth and development.

Description

Technical Field

[0001] This utility model relates to the field of silver ear fungus production technology, and in particular to a silver ear fungus liquid spawn inoculation device. Background Technology

[0002] Compared to solid spores of Tremella fuciformis, liquid spores have advantages such as good fluidity, easy dispersion, rapid germination, and numerous mycelial growth points. This effectively solves the problems of slow germination and easy contamination during inoculation, shortening the cultivation time and facilitating industrial production. Furthermore, liquid spores are not graded and can be used as mother cultures for primary culture production or directly as cultivation stock for production.

[0003] Inoculation of liquid cultures still requires manual intervention, which is costly, labor-intensive, and inefficient. The more people involved, the greater the chance of contamination, which is not conducive to complete sterility in the production process. At the same time, manual operation has problems with poor accuracy in controlling the inoculation amount and inconsistent inoculation amounts for different culture bottles. Utility Model Content

[0004] In view of this, the purpose of this utility model is to provide a convenient and practical inoculation device for liquid tremella fuciformis, which improves production efficiency, saves labor, and improves inoculation quality.

[0005] This utility model is achieved by the following scheme: a liquid cultivar inoculation device for tremella includes a conveying device for conveying a tray containing culture bottles and an inoculation mechanism located above the conveying device; the inoculation mechanism includes an inoculation plate frame located above the conveying device and which can be raised and lowered, the inoculation plate frame is provided with inoculation tubes corresponding one-to-one with the culture bottles on the tray, and an inoculation control device for conveying tremella liquid cultivar to the inoculation tubes is provided above the inoculation plate frame.

[0006] Furthermore, the inoculation control device includes a control frame fixedly connected above the inoculation plate frame. A dispensing cylinder is horizontally arranged on the top of the control frame, and a plurality of dispensing ports are opened at the bottom of the dispensing cylinder. The dispensing ports are connected to the inoculation tubes one by one through dispensing hoses. A first intercepting device, an inoculation volume control device, and a second intercepting device are arranged sequentially from top to bottom on the control frame. The dispensing hose passes through the first intercepting device, the inoculation volume control device, and the second intercepting device in sequence.

[0007] Furthermore, the first intercepting device includes a first fixed crossbar and a first movable pressure bar that cooperate to clamp the feeding hose; the inoculation amount control device includes a fixed pressure plate and a movable pressure plate that cooperate to clamp the feeding hose; the second intercepting device includes a second fixed crossbar and a second movable pressure bar that cooperate to clamp the feeding hose.

[0008] Furthermore, the first movable pressure rod is driven by cylinder A located behind the first fixed crossbar, the movable pressure plate is driven by cylinder B located behind the fixed pressure plate, and the second movable pressure rod is driven by cylinder C located behind the second fixed crossbar.

[0009] Furthermore, the inoculation plate frame is driven to rise and fall by the first lifting cylinders on both sides of the conveying device.

[0010] Furthermore, the top of the mixing cylinder is provided with an air vent, and the upper end of the air vent is connected to a buffer container, and the upper end of the buffer container is provided with an air outlet.

[0011] Furthermore, it also includes a storage tank containing liquid tremella fuciformis culture, the storage tank having a discharge port, and a liquid delivery pipe connected between the discharge port and the mixing cylinder; a high-pressure air pipe is connected to the upper part of the storage tank, and a filter is connected in series on the high-pressure air pipe.

[0012] Compared with the prior art, the present invention has the following beneficial effects: The present invention's Tremella liquid spawn inoculation machine is convenient to use and highly practical, realizing mechanized inoculation of Tremella liquid spawn, which not only improves production efficiency, reduces labor intensity, saves manpower, reduces the number of personnel entering the sterile room, and reduces the risk of spawn contamination; at the same time, it can stably control the inoculation amount of liquid spawn, improve the accuracy of the inoculation amount, make the inoculation amount more uniform and consistent, improve the inoculation quality, and is conducive to the later mycelial growth and development.

[0013] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below through specific embodiments and related drawings. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the usage state of an embodiment of this utility model;

[0015] Figure 2 This is a front view of the vaccination mechanism according to an embodiment of this utility model;

[0016] Explanation of the numbers in the diagram: 100-Conveying device, 110-Lifting stop, 120-Second lifting cylinder, 200-Inoculation mechanism, 210-Inoculation plate frame, 220-Inoculation tube, 230-Control frame, 231-First fixed crossbar, 232-First movable pressure bar, 233-Fixed pressure plate, 234-Modular pressure plate, 235-Second fixed crossbar, 236-Second movable pressure bar, 240-Dispensing cylinder, 250-Dispensing hose, 260-First lifting cylinder, 270-Buffer container, 300-Tray, 310-Cultivation bottle, 400-Storage tank, 410-Infusion tube, 420-High-pressure air tube. Detailed Implementation

[0017] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0018] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0019] like Figures 1-2 As shown, a liquid cultivar inoculation device for *Tremella fuciformis* includes a conveying device 100 for conveying a tray 300 containing culture bottles 310 and an inoculation mechanism 200 located above the conveying device 100. The inoculation mechanism 200 includes an inoculation plate 210 located above the conveying device and capable of being raised and lowered. The inoculation plate 210 is provided with inoculation tubes 220 corresponding one-to-one with the culture bottles 310 on the tray 300. An inoculation control device is located above the inoculation plate 210 to deliver liquid cultivar cultivar 220 to the inoculation tubes. Culture bottles containing culture medium (with caps removed) are arranged in an array on the tray. The conveying device transports the tray to below the inoculation mechanism 200. The inoculation mechanism injects a fixed amount of liquid cultivar 220 into the inoculation tanks within the culture bottles through the inoculation tubes. The inoculation control device controls the feeding and the feeding amount. This utility model of a liquid cultivar inoculation machine for tremella is convenient to use and highly practical. It realizes mechanized inoculation of liquid cultivar in tremella, which not only improves production efficiency, reduces labor intensity, saves manpower, reduces the number of personnel entering the sterile room, and reduces the risk of cultivar contamination, but also allows for stable control of the inoculation amount, improves the accuracy of the inoculation amount, makes the inoculation amount more uniform, improves the inoculation quality, and is conducive to the subsequent mycelial growth and development.

[0020] In this embodiment, the culture flasks on the tray are arranged in a 4x4 array, and the inoculation tubes are also arranged in a 4x4 array, corresponding one-to-one with the positions of the culture flasks. Two sets of inoculation control devices are installed above the inoculation plate rack 210. Each set of inoculation control devices is connected to 8 inoculation tubes for inoculating 8 culture flasks, and the two sets of inoculation control devices work synchronously.

[0021] In this embodiment, the inoculation control device includes a control frame 230 fixedly connected above the inoculation plate rack. A dispensing cylinder 240 is horizontally arranged at the top of the control frame, and several dispensing ports are opened at the bottom of the dispensing cylinder. Each dispensing port is connected to an inoculation tube 220 via a dispensing hose 250. From top to bottom, the control frame is equipped with a first intercepting device, an inoculation volume control device, and a second intercepting device. The dispensing hose passes through the first intercepting device, the inoculation volume control device, and the second intercepting device in sequence. The liquid inoculum in the dispensing cylinder 240 flows into the inoculation tube through the dispensing hose, and the first intercepting device, the inoculation volume control device, and the second intercepting device control the dispensing and the dispensing volume.

[0022] In this embodiment, the first interception device includes a first fixed crossbar 231 and a first movable pressure bar 232 that cooperate to clamp the feed hose; the inoculation amount control device includes a fixed pressure plate 233 and a movable pressure plate 234 that cooperate to clamp the feed hose; the second interception device includes a second fixed crossbar 235 and a second movable pressure bar 236 that cooperate to clamp the feed hose. When the first movable pressure bar 232 moves toward the first fixed crossbar 231, the two cooperate to clamp the feed hose to achieve interception; when the second movable pressure bar 236 moves toward the second fixed crossbar 235, the two cooperate to clamp the feed hose to achieve interception; when the movable pressure plate 234 moves toward the fixed pressure plate 233, the two cooperate to clamp the feed hose, thereby squeezing the feed hose and forcing the liquid inoculum in the feed hose into the culture bottle. Specific operation process: (1) The first intercepting device clamps the upper part of the feeding hose to block the upper part of the feeding hose, and then the second intercepting device is released. At this time, the liquid bacteria in the feeding hose will not flow down; (2) The inoculation amount control device clamps the middle part of the feeding hose and squeezes the middle part of the feeding hose to squeeze the predetermined amount of liquid bacteria into the culture bottle; (3) The second intercepting device clamps the lower part of the feeding hose to block the lower part of the feeding hose, and then the first intercepting device and the inoculation amount control device are released. The liquid bacteria in the mixing cylinder continues to fill the feeding hose, and the inoculation operation is completed. By changing the movable pressure plate 234 of different widths, the length of the middle part of the feeding hose squeezed by the inoculation amount control device can be changed, thereby adjusting the amount of liquid bacteria (i.e., the inoculation amount). This inoculation control device can simultaneously control all feeding tubes to move synchronously.

[0023] In this embodiment, the culture flasks on the tray are arranged in a 4*4 array, and two sets of inoculation control devices are set above the inoculation plate rack 210. Each set of inoculation control devices is connected to 8 inoculation tubes for inoculating 8 culture flasks.

[0024] In this embodiment, the inoculation plate frame is driven to rise and fall by the first lifting cylinders 260 on both sides of the conveying device; the first movable pressure rod is driven by cylinder A located behind the first fixed crossbar, and the telescopic rod of cylinder A passes through the first fixed crossbar and connects to the first movable pressure rod; the movable pressure plate is driven by cylinder B located behind the fixed pressure plate, and the telescopic rod of cylinder B passes through the fixed pressure plate and connects to the movable pressure plate; the second movable pressure rod is driven by cylinder C located behind the second fixed crossbar, and the telescopic rod of cylinder C passes through the second fixed crossbar and connects to the second movable pressure rod.

[0025] In this embodiment, in order to remove air from the liquid bacterial culture, an air vent is provided at the top of the mixing cylinder, and a buffer container 270 is connected to the upper end of the air vent. The upper end of the buffer container is provided with an air outlet, which can be equipped with a valve.

[0026] In this embodiment, a storage tank 600 containing liquid tremella fuciformis culture is also included. The storage tank is provided with a discharge port, and a delivery pipe 610 is connected between the discharge port and the mixing cylinder. A high-pressure air pipe 620 is connected to the upper part of the storage tank, and a filter is connected in series on the high-pressure air pipe. The pressure of high-pressure air is used to squeeze the liquid tremella fuciformis culture in the storage tank into the mixing cylinder. The high-pressure air is filtered to avoid introducing impurities or bacteria. At the same time, compared with the pumping method, it can better protect the culture.

[0027] In this embodiment, the conveying device 100 is a roller conveyor. The conveying device is provided with a lifting block 110 located below the inoculation mechanism. The lifting block is driven to rise and fall by a second lifting cylinder 120 located below it. The lifting block restricts the position of the tray and prevents the tray from moving during the inoculation process. After the inoculation is completed, the lifting block descends and the tray continues to move forward along the conveying device.

[0028] Unless otherwise stated, if any of the technical solutions disclosed in this utility model discloses a numerical range, then the disclosed numerical range is a preferred numerical range. Any person skilled in the art should understand that the preferred numerical range is merely one among many feasible numerical values ​​that has a more obvious or representative technical effect. Because there are many numerical values, it is impossible to list them all. Therefore, this utility model discloses only some numerical values ​​to illustrate the technical solutions of this utility model. Furthermore, the numerical values ​​listed above should not constitute a limitation on the scope of protection of this utility model.

[0029] If this utility model discloses or relates to mutually fixedly connected parts or structural components, then unless otherwise stated, a fixed connection can be understood as: a detachable fixed connection (e.g., using bolts or screws), or a non-detachable fixed connection (e.g., riveting, welding). Of course, mutually fixed connections can also be replaced by an integral structure (e.g., manufactured by integral molding using a casting process) (except where it is obviously impossible to use an integral molding process).

[0030] In addition, unless otherwise stated, the terms used to indicate positional relationships or shapes in any of the technical solutions disclosed in this utility model above include states or shapes that are similar to, close to, or approximate with them.

[0031] Any component provided by this utility model can be assembled from multiple individual components, or it can be a single component manufactured by a one-piece molding process.

[0032] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from its technical solution shall still fall within the protection scope of this utility model.

Claims

1. A device for inoculating Tremella fuciformis liquid culture, characterized in that: It includes a conveying device for conveying a tray containing culture bottles and an inoculation mechanism located above the conveying device; the inoculation mechanism includes an inoculation plate frame located above the conveying device and which can be raised and lowered, the inoculation plate frame is provided with inoculation tubes corresponding one-to-one with the culture bottles on the tray, and an inoculation control device is provided above the inoculation plate frame for conveying liquid tremella fuciformis culture to the inoculation tubes.

2. The Tremella fuciformis liquid spawn inoculation device according to claim 1, characterized in that: The inoculation control device includes a control frame fixedly connected above the inoculation plate frame. A dispensing cylinder is horizontally arranged on the top of the control frame, and several dispensing ports are opened at the bottom of the dispensing cylinder. The dispensing ports are connected to the inoculation tubes one by one through dispensing hoses. A first intercepting device, an inoculation volume control device, and a second intercepting device are arranged sequentially from top to bottom on the control frame. The dispensing hose passes through the first intercepting device, the inoculation volume control device, and the second intercepting device in sequence.

3. The Tremella fuciformis liquid spawn inoculation device according to claim 2, characterized in that: The first intercepting device includes a first fixed crossbar and a first movable pressure bar that cooperate to clamp the feeding hose; the inoculation amount control device includes a fixed pressure plate and a movable pressure plate that cooperate to clamp the feeding hose; the second intercepting device includes a second fixed crossbar and a second movable pressure bar that cooperate to clamp the feeding hose.

4. The Tremella fuciformis liquid spawn inoculation device according to claim 3, characterized in that: The first movable pressure rod is driven by cylinder A located behind the first fixed crossbar, the movable pressure plate is driven by cylinder B located behind the fixed pressure plate, and the second movable pressure rod is driven by cylinder C located behind the second fixed crossbar.

5. The Tremella fuciformis liquid spawn inoculation device according to claim 1, characterized in that: The inoculation plate frame is raised and lowered by the first lifting cylinders on both sides of the conveying device.

6. The Tremella fuciformis liquid spawn inoculation device according to claim 2, characterized in that: The top of the mixing cylinder is provided with an air vent, and the upper end of the air vent is connected to a buffer container, and the upper end of the buffer container is provided with an air outlet.

7. The Tremella fuciformis liquid spawn inoculation device according to claim 2, characterized in that: It also includes a storage tank containing liquid tremella fuciformis culture, the storage tank having a discharge port, and a liquid delivery pipe connected between the discharge port and the mixing cylinder; a high-pressure air pipe is connected to the upper part of the storage tank, and a filter is connected in series on the high-pressure air pipe.

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