Composition and method for detection of biomolecules via aromatic labeling using heterocyclic compounds
The use of a heterocyclic compound that binds to aromatic amino acid residues in biomolecules addresses the limitations of current protein visualization methods by enabling rapid, sensitive, and minimally invasive protein detection, preserving protein integrity for further analysis.
WO2025129105A1 Publication Date: 2025-06-19LIFE TECHNOLOGIES CORP +1
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Patent Information
- Application Number
- PCT/US2024/060183
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2023-12-15
- Filing Date
- 2024-12-13
- Publication Date
- 2025-06-19
AI Technical Summary
Technical Problem
Current methods for protein visualization, such as gel electrophoresis, require lengthy staining and destaining procedures, are sensitive to protein handling, and can interfere with downstream applications like western blotting.
Method used
A composition comprising a heterocyclic compound that selectively binds to aromatic amino acid residues of biomolecules, generating a fluorescent signal upon association, allowing for rapid and sensitive protein detection without excessive handling or protein modification.
Benefits of technology
Enables fast and efficient protein visualization with high sensitivity and minimal protein modification, preserving protein integrity for downstream analysis and reducing the need for extensive staining procedures.
✦ Generated by Eureka AI based on patent content.
Abstract
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
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