Method for forming conjugate of target substance and peptide

By using a linker with multiple puromycin-like substances to form a conjugate with target mRNA, the method addresses the limitations of single-binding nucleic acid translation products, enhancing peptide selection and binding efficiency.

WO2026089003A1 Publication Date: 2026-04-30PEPTIDREAM INC
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Patent Information

Application Number
PCT/JP2025/037241
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-10-25
Filing Date
2025-10-23
Publication Date
2026-04-30

AI Technical Summary

Technical Problem

Existing puromycin-like substance-mediated display methods only allow for a single nucleic acid to be bound to a monovalent translation product, limiting the efficiency and versatility of peptide selection and binding.

Method used

A linker with multiple puromycin-like substances is used to form a conjugate with a target mRNA, allowing multiple translation products to be bound to a single mRNA, which is then synthesized in a cell-free translation system, resulting in a conjugate where multiple peptides are linked to a single nucleic acid.

Benefits of technology

This approach enhances the efficiency of peptide selection and binding by enabling multiple peptides to interact with target substances, increasing the binding ability and versatility of the conjugate.

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Abstract

The present invention relates to a method for forming a conjugate of a desired target substance and a peptide. A method according to the present invention includes a step for bringing a genetic information material-linker-peptide conjugate into contact with a target substance conjugate containing at least two target substances. The genetic information material-linker-peptide conjugate includes: (a) a linker that includes a bond portion having a structure capable of binding to a desired genetic information material and includes at least two puromycin-like substances, the puromycin-like substances being capable of covalently binding to the C-terminus of a desired peptide; (b) a genetic information material that is bonded to the bond portion of the linker of (a); and (c) a peptide that is bonded to at least two or more puromycin-like substances of the linker of (a) and is encoded by the genetic information material.
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