Method for preparing 10-deacetyl asperulosidic acid methyl ester
Through the method of multi-step extraction and chromatographic separation combined with crystallization, the problem of complex preparation and low yield of desacetylated methyl trifoliate in the existing technology is solved, a high-purity and efficient preparation process is achieved, the process flow is simplified and the efficiency is improved. improve extraction efficiency.
Patent Information
- Application Number
- CN200610027531.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2006-06-10
- Publication Date
- 2007-12-12
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing preparation method of deacetylated methyl trifoliate is complex and has low yield. It uses flammable solvents and expensive equipment, and the process is not simple.
Organic solvents are used to extract and separate botanical medicinal materials containing deacetylated methyl trifoliate, separated by silica gel column and gel column chromatography, combined with crystallization and recrystallization, using common solvents such as ethanol, n-hexane, ethyl acetate and n-butanol. Multi-step extraction, final separation by diatomaceous earth and Sephadex gel column chromatography, gradient elution to obtain high purity products.
The preparation process is simplified, the reproducibility and purity are improved, and the purity can reach more than 98%. Common solvents are used, the process flow is simplified, and the extraction efficiency is improved.