Preparation method of gardenia extract

Abstract

The invention provides a preparation method of a gardenia extract, which combines the adoption of activated carbon and separation of macroporous resin and comprises the following steps: first washing activated carbon of which the usage amount is 1-3 times with water, and then eluting the activated carbon with 50%-80% ethanol, wherein the eluting flow rate is 0.6-1 time of crude drug amount/hour; collecting and concentrating the ethanol eluent to be put on a macroporous resin column, wherein the sampling volume is 1-3 times of column volume; adsorbing at the flow rate of 0.2-1.0 time of columnvolume/hour; eluting with 1-3 times of column volume of water and 3%-10% ethanol with 1-3 times of column volume; eluting with 20%-50% ethanol with 3-8 times of column volume at the eluting flow rateof 0.2-0.5 time of column volume/hour; and collecting 20%-50% ethanol eluent. The invention has high extraction efficiency and low cost and is suitable for industrialized preparation.

Description

technical field

[0001] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of a gardenia extract. Background technique

[0002] At present, the extraction method of geniposide generally uses organic solvents such as chloroform and absolute ethanol to extract in a Soxhlet extraction bottle to obtain the total active ingredient geniposide in gardenia, and then separate it on a silica gel column. methanol, chloroform mixed solution elution, the eluent is recrystallized in acetone to obtain geniposide crystals, generally about 4.0 g of geniposide can be separated from 100 g of gardenia fruit, but this method is not suitable for industrial production. Only experimental production is possible.

[0003] According to current literature reports, in the gardenia jasminoides extraction method with higher geniposide content, ethanol reflux extraction is often used, but ethanol reflux extraction has limitations, ...

Examples

Embodiment 1

[0305] Take 50g of gardenia medicinal material, add 10 times the amount of water, decoct and extract 3 times, each time for 1.5 hours, filter, combine the filtrate, concentrate to a relative density of 1.05 (60°C), add 95% ethanol to increase the alcohol content of the liquid reach 70%, put it aside for 24 hours, filter with suction, wash the precipitate with 3 times the amount of crude drug in 70% ethanol solution, merge the filtrate with the lotion, recycle the ethanol until the medicinal solution has no alcohol smell, add water to adjust the concentration of the medicinal solution to medicinal material: medicinal solution = 1: 1, namely 1g / ml. Activated carbon is purified by static adsorption method: the activated carbon is refluxed with ethanol until there is no absorption in ultraviolet scanning, and washed with distilled water until it contains no alcohol smell. The amount of sample loaded is crude drug: activated carbon = 1:1.2, put the specified amount of activated car...

Embodiment 2

[0306] Embodiment 2: capsule

[0307] Get 100 g of the gardenia active ingredient prepared in Example 1 and prepare it into capsules through a conventional process.

Embodiment 3

[0309] Take 50g of gardenia medicinal material, add 9 times the amount of water, decoct and extract 4 times, each time for 1.5 hours, filter, combine the filtrate, concentrate to a relative density of 1.05 (60°C), add 95% ethanol to increase the alcohol content of the liquid reach 65%, put it aside for 24 hours, filter with suction, wash the precipitate with 3 times the amount of crude drug in 70% ethanol solution, combine the filtrate with the lotion, recycle the ethanol until the medicinal solution has no alcohol smell, add water to adjust the concentration of the medicinal solution to medicinal material: medicinal solution = 1: 1, namely 1g / ml. Activated carbon is purified by static adsorption method: the activated carbon is refluxed with ethanol until there is no absorption in ultraviolet scanning, and washed with distilled water until it contains no alcohol smell. The amount of sample loaded is crude drug: activated carbon = 1:1.3, put the specified amount of activated ca...

technical field

[0001] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of a gardenia extract. Background technique

[0002] At present, the extraction method of geniposide generally uses organic solvents such as chloroform and absolute ethanol to extract in a Soxhlet extraction bottle to obtain the total active ingredient geniposide in gardenia, and then separate it on a silica gel column. methanol, chloroform mixed solution elution, the eluent is recrystallized in acetone to obtain geniposide crystals, generally about 4.0 g of geniposide can be separated from 100 g of gardenia fruit, but this method is not suitable for industrial production. Only experimental production is possible.

[0003] According to current literature reports, in the gardenia jasminoides extraction method with higher geniposide content, ethanol reflux extraction is often used, but ethanol reflux extraction has limitations, ...

Examples

Embodiment 1

[0305] Take 50g of gardenia medicinal material, add 10 times the amount of water, decoct and extract 3 times, each time for 1.5 hours, filter, combine the filtrate, concentrate to a relative density of 1.05 (60°C), add 95% ethanol to increase the alcohol content of the liquid reach 70%, put it aside for 24 hours, filter with suction, wash the precipitate with 3 times the amount of crude drug in 70% ethanol solution, merge the filtrate with the lotion, recycle the ethanol until the medicinal solution has no alcohol smell, add water to adjust the concentration of the medicinal solution to medicinal material: medicinal solution = 1: 1, namely 1g / ml. Activated carbon is purified by static adsorption method: the activated carbon is refluxed with ethanol until there is no absorption in ultraviolet scanning, and washed with distilled water until it contains no alcohol smell. The amount of sample loaded is crude drug: activated carbon = 1:1.2, put the specified amount of activated car...

Embodiment 2

[0306] Embodiment 2: capsule

[0307] Get 100 g of the gardenia active ingredient prepared in Example 1 and prepare it into capsules through a conventional process.

Embodiment 3

[0309] Take 50g of gardenia medicinal material, add 9 times the amount of water, decoct and extract 4 times, each time for 1.5 hours, filter, combine the filtrate, concentrate to a relative density of 1.05 (60°C), add 95% ethanol to increase the alcohol content of the liquid reach 65%, put it aside for 24 hours, filter with suction, wash the precipitate with 3 times the amount of crude drug in 70% ethanol solution, combine the filtrate with the lotion, recycle the ethanol until the medicinal solution has no alcohol smell, add water to adjust the concentration of the medicinal solution to medicinal material: medicinal solution = 1: 1, namely 1g / ml. Activated carbon is purified by static adsorption method: the activated carbon is refluxed with ethanol until there is no absorption in ultraviolet scanning, and washed with distilled water until it contains no alcohol smell. The amount of sample loaded is crude drug: activated carbon = 1:1.3, put the specified amount of activated ca...

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