Method for extracting mangiferin from mango leaf

Through the method of combining alkaline water bath extraction and acidification, combined with alcohol elution and column chromatography technology, the yield and purity of mangiferin are improved, solving the problems of low extraction efficiency and insufficient purity in the existing technology, and achieving high efficiency , environmentally friendly mangiferin extraction process.

CN101993437AInactive Publication Date: 2011-03-30SHANGHAI NORMAL UNIVERSITY
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Patent Information

Application Number
CN200910194672.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2009-08-27
Publication Date
2011-03-30
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

It is difficult to effectively increase the yield and purity of mangiferin in mango leaves with existing technology, and the extraction process is complex and has a large impact on the environment.

Method used

The alkaline water bath extraction method is used, combined with acidification, alcohol elution and recrystallization, and Sephadex LH-20 column chromatography is used to extract mangiferin using alkaline solutions such as sodium hydroxide and potassium hydroxide, and by adjusting the extraction conditions such as time, temperature and elution steps to improve the extraction rate and purity of mangiferin.

Benefits of technology

The extraction rate of mangiferin has reached more than 60%, and the purity has reached more than 96%. The process is simple, the reagents used have low toxicity and little impact on the environment, and the process can be reused.

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Abstract

The invention relates to a method for extracting mangiferin from mango leaves, belonging to the field of drug preparation. The method enhances the output of the mangiferin through a reasonable extraction process. The method sequentially comprises the following steps of: previously grinding the mango leaves into mango leaf coarse powder; then carrying out tepid extraction on the mango leaf coarse powder by using an alkaline water solution; carrying out filtration treatment on an obtained extracting solution, and then acidizing the extracting solution by using a dilute acid; concentrating; adding alcohol till the content of the alcohol is 10-30 percent; centrifugalizing; processing a centrifugalized supernate by using a Sephadex LH-20 column; firstly washing with water, and then eluting with alcohol with the mass percentage concentration of 90-95 percent; drying an eluent to dryness by evaporating, and then carrying out alcohol recrystallization to obtain the mangiferin. The invention has simple and convenient method, low cost and high yield; in addition, the obtained mangiferin has high purity.
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