Corn dehydration responsive element (DRE)-binding protein ZmDBP2 and encoding gene thereof

Through the newly discovered corn dehydration response element binding protein ZmDBP2 and its encoding gene, the DRE/CRT cis-element is regulated, which solves the problem of insufficient corn resistance to stress stress in the existing technology and achieves the improvement of stress resistance and improvement in corn. Effects of stress tolerance.

CN102140134AInactive Publication Date: 2011-08-03BEIJING TECH & BUSINESS UNIV
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Patent Information

Application Number
CN201110025364.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2011-01-24
Publication Date
2011-08-03
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

It is difficult to effectively improve the resistance of corn to drought, high salt and high temperature stress with existing technology, and it is difficult to improve the comprehensive stress resistance of plants with a single functional protein gene.

Method used

Provides a newly discovered dehydration response element binding protein ZmDBP2 in corn and its encoding gene, which can be expressed under drought, high salt and low temperature conditions, and specifically regulate the transcriptional expression of genes containing DRE/CRT cis elements to promote stress resistance. and expression of stress tolerance-related genes.

Benefits of technology

It enhances the resistance of corn to adverse stress, provides a basis for improving plant resistance and stress tolerance in breeding, and improves the overall stress resistance of plants by regulating the expression of multiple functional genes.

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Abstract

The invention discloses a dehydration responsive element (DRE)-binding protein and an encoding gene thereof. The DRE-binding protein is a protein having an amino acid residue sequence shown as SEQ ID No:2 in a sequence table or a protein, which is obtained by substituting, deleting or adding one or more amino acid residues on the amino acid residue sequence shown as SEQ ID No:2, has the same activity as that of the amino acid residue sequence shown as SEQ ID No:2 and is derived from the SEQ ID No:2. An experiment proves that the ZmDBP2 disclosed by the invention is expressed under the induction of drought, high salt, low temperature and ABA (Abscisic Acid), and the transcription expression of a gene containing a DRE / CRT (Calreticulin) cis element (core sequence: CCGAC) can be regulated and controlled specifically. By adopting the ZmDBP2, a basis is laid for manual control over the expression of a stress resistance and stress tolerance related gene. The ZmDBP2 is about to play an important role in cultivating plant breeds with enhanced stress resistance and stress tolerance.
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