Antitumor compound extracted from guttifer, and preparation method and application thereof

By extracting and separating compounds from Garcinia cambogia and combining them with pharmaceutical ingredients to prepare anti-tumor drugs, the problem of limited therapeutic effect of existing anti-tumor drugs has been solved, and the treatment of tumors such as glioblastoma, lung adenocarcinoma and ovarian cancer has been achieved. effective suppression.

CN103694248AActive Publication Date: 2014-04-02JIANGSU KANION PHARMA CO LTD
4 Cites 2 Cited by

Patent Information

Application Number
CN201310654337.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2013-11-28
Publication Date
2014-04-02
Estimated Expiration
2033-11-28

AI Technical Summary

Technical Problem

Existing anti-tumor drugs have limited therapeutic effects on a variety of tumor diseases, especially in glioblastoma, lung adenocarcinoma, and ovarian cancer, and there is a lack of effective treatment options.

Method used

A biologically active compound was extracted and isolated from Garcinia cambogia. The compound was prepared through ethyl acetate extraction, silica gel column chromatography, HPLC-PDA-MS analysis and reversed-phase RP-18 chromatography, and combined with medicine. Preparation of anti-tumor drugs from scientifically acceptable inactive ingredients.

Benefits of technology

The compound shows broad-spectrum inhibitory activity against tumor cells such as glioma, lung adenocarcinoma and ovarian cancer, has significant anti-tumor effects, and provides a new drug option for the treatment of tumor diseases.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention relates to an antitumor compound extracted from guttifer, and a preparation method and application thereof. The preparation method of the compound comprises the following steps: (1) taking the medicinal material guttifer, extracting with ethyl acetate, carrying out silica gel column chromatographic separation on the extract, and carrying out petroleum ether-ethyl acetate gradient elution to obtain 11 components A-K; (2) discarding the grease components and garcinia acid components, carrying out HPLC-PDA-MS (high performance liquid chromatography-potato dextrose agar-Murashige-Skoog) repeat analysis to obtain a key component I; (3) carrying out silica gel column chromatographic separation on the component I, carrying out chloroform-methanol gradient elution to obtain a fraction I-2, collecting one fraction for every 30ml, and merging identical components according to the TLC (thin layer chromatography) result to obtain a fraction I-3; (4) carrying out reversed phase RP-18 separation on I-3, carrying out acetonitrile-water gradient elution, collecting one fraction for every 20ml, and merging identical components to obtain a subfraction I-3E; and (5) carrying out purification and acetonitrile elution on I-3E to obtain the compound. The compound can be used for treating tumors.
Need to check novelty before this filing date? Find Prior Art