Sugarcane root-promoting and seedling-promoting medium and method for direct field planting of sugarcane tissue culture bottle seedlings

Abstract

The provides a root-inducing strong seedling medium for sugarcane. The medium is prepared from a mixture I containing a lot of elements, a mixture II containing trace elements, a mixture of iron salts, an organic mixture, a plant hormone and sucrose by using agar as a curing agent. In particular, the mixture I comprises (800-1000) mg / L KNO3, (650-800) mg / L Ca(NO3)2.4H2O, (500-650) mg / L NH4NO3, (100-150) mg / L KH2PO4, (170-200) mg / L MgSO4.7H2O and (60-80) mg / L CaCl2.2H2O. The medium provided by the invention can substantially strengthen sugarcane tissue culture plants and promote root growth. The invention also provides a direct field planting method of sugarcane tissue culture bottle seedlings. The method includes the steps of: 1, dispensing the medium in glass test tubes; 2, inoculating tissue culture differentiated seedlings into the glass test tubes, sealing and culturing; and 3, successively conducting hardening and field planting after tissue culture differentiated seedling culture. The method provided by the invention has the characteristics of few steps and simple operation.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and more precisely relates to a culture method for sugarcane tissue culture medium and sugarcane bottle seedlings. Background technique

[0002] Sugarcane is a vegetatively propagated crop. It has always been propagated by stem buds in production. The amount of seeds used is large, which increases production costs and has a low reproduction coefficient. Both adopt the continuous cropping system, and the sugarcane suffers from diseases for a long time, which seriously reduces the yield and quality, and accelerates the degeneration of varieties.

[0003] Sugarcane tissue culture is an efficient propagation technique and an effective way to obtain non-toxic sugarcane seedlings, which plays an important role in the sugarcane industry. In Brazil, Cuba and other countries, 90% of sugarcane areas have realized tissue culture technology to cultivate virus-free seedlings, multiply improved v...

Examples

Embodiment 1

[0030] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0031] Mixture I with a large number of elements including KNO 3 800 mg / L, Ca(NO 3 ) 2 4H 2 O 650 mg / L, NH 4 NO 3 650 mg / L, KH 2 PO 4 100 mg / L, MgSO 4 ·7H 2 O 170 mg / L and CaCl 2 2H 2 O 60 mg / L, the molar concentration ratio of nitrate nitrogen to ammonium nitrogen was 2.65:1, and the total nitrogen content was 29.7 mmol / L.

[0032] Mixture II with trace elements includes H 3 BO 3 2 mg / L, MnSO 4 4H2 O 8 mg / L, ZnSO 4 ·7H 2 O3 mg / L, NaMoO 4 2H 2 O 0.1 mg / L, KI 0.3 mg / L.

[0033] The iron salt mixture includes Na 2 -EDTA 16 mg / L, FeSO 4 ·7H 2 O 12 mg / L and Na 2 SiO 3 9H 2 O 24mg / L.

[0034] The organic mixture included inositol 37 mg / L, glycine 0.3 mg / L, thiamine hydro...

Embodiment 2

[0039] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0040] Mixture I with a large number of elements including KNO 3 900 mg / L, Ca(NO 3 ) 2 4H 2 O 720 mg / L, NH 4 NO 3 600 mg / L, KH 2 PO 4 125 mg / L, MgSO 4 ·7H 2 O 185 mg / L and CaCl 2 2H 2 O 70 mg / L, the molar concentration ratio of nitrate nitrogen and ammonium nitrogen is 3:1, and the total nitrogen content is 30 mmol / L.

[0041] Mixture II with trace elements includes H 3 BO 3 3 mg / L, MnSO 4 4H 2 O 11.5 mg / L, ZnSO 4 ·7H 2 O 4.5 mg / L, NaMoO 4 2H 2 O 0.15 mg / L, KI 0.4 mg / L.

[0042] The iron salt mixture includes Na 2 -EDTA 19 mg / L, FeSO 4 ·7H 2 O 14 mg / L and Na 2 SiO 3 9H 2 O 28mg / L.

[0043] The organic mixture included inositol 50 mg / L, glycine 0.45 mg / L, thiamine hy...

Embodiment 3

[0048] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0049] Mixture I with a large number of elements including KNO 3 1000 mg / L, Ca(NO 3 ) 2 4H 2 O 800 mg / L, NH 4 NO 3 500 mg / L, KH 2 PO 4 150 mg / L, MgSO 4 ·7H 2 O 200 mg / L and CaCl 2 2H 2 O 80 mg / L, the molar concentration ratio of nitrate nitrogen and ammonium nitrogen is 3.67:1, and the total nitrogen content is 29.2mmol / L.

[0050] Mixture II with trace elements includes H 3 BO 3 4 mg / L, MnSO 4 4H 2 O 15 mg / L, ZnSO 4 ·7H 2 O 6 mg / L, NaMoO 4 2H 2 O 0.2 mg / L, KI 0.5 mg / L.

[0051] The iron salt mixture includes Na 2 -EDTA 22 mg / L, FeSO 4 ·7H 2 O 16 mg / L and Na 2 SiO 3 9H 2 O 33mg / L.

[0052] The organic mixture included inositol 65 mg / L, glycine 0.6 mg / L, thiamine h...

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and more precisely relates to a culture method for sugarcane tissue culture medium and sugarcane bottle seedlings. Background technique

[0002] Sugarcane is a vegetatively propagated crop. It has always been propagated by stem buds in production. The amount of seeds used is large, which increases production costs and has a low reproduction coefficient. Both adopt the continuous cropping system, and the sugarcane suffers from diseases for a long time, which seriously reduces the yield and quality, and accelerates the degeneration of varieties.

[0003] Sugarcane tissue culture is an efficient propagation technique and an effective way to obtain non-toxic sugarcane seedlings, which plays an important role in the sugarcane industry. In Brazil, Cuba and other countries, 90% of sugarcane areas have realized tissue culture technology to cultivate virus-free seedlings, multiply improved v...

Examples

Embodiment 1

[0030] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0031] Mixture I with a large number of elements including KNO 3 800 mg / L, Ca(NO 3 ) 2 4H 2 O 650 mg / L, NH 4 NO 3 650 mg / L, KH 2 PO 4 100 mg / L, MgSO 4 ·7H 2 O 170 mg / L and CaCl 2 2H 2 O 60 mg / L, the molar concentration ratio of nitrate nitrogen to ammonium nitrogen was 2.65:1, and the total nitrogen content was 29.7 mmol / L.

[0032] Mixture II with trace elements includes H 3 BO 3 2 mg / L, MnSO 4 4H2 O 8 mg / L, ZnSO 4 ·7H 2 O3 mg / L, NaMoO 4 2H 2 O 0.1 mg / L, KI 0.3 mg / L.

[0033] The iron salt mixture includes Na 2 -EDTA 16 mg / L, FeSO 4 ·7H 2 O 12 mg / L and Na 2 SiO 3 9H 2 O 24mg / L.

[0034] The organic mixture included inositol 37 mg / L, glycine 0.3 mg / L, thiamine hydro...

Embodiment 2

[0039] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0040] Mixture I with a large number of elements including KNO 3 900 mg / L, Ca(NO 3 ) 2 4H 2 O 720 mg / L, NH 4 NO 3 600 mg / L, KH 2 PO 4 125 mg / L, MgSO 4 ·7H 2 O 185 mg / L and CaCl 2 2H 2 O 70 mg / L, the molar concentration ratio of nitrate nitrogen and ammonium nitrogen is 3:1, and the total nitrogen content is 30 mmol / L.

[0041] Mixture II with trace elements includes H 3 BO 3 3 mg / L, MnSO 4 4H 2 O 11.5 mg / L, ZnSO 4 ·7H 2 O 4.5 mg / L, NaMoO 4 2H 2 O 0.15 mg / L, KI 0.4 mg / L.

[0042] The iron salt mixture includes Na 2 -EDTA 19 mg / L, FeSO 4 ·7H 2 O 14 mg / L and Na 2 SiO 3 9H 2 O 28mg / L.

[0043] The organic mixture included inositol 50 mg / L, glycine 0.45 mg / L, thiamine hy...

Embodiment 3

[0048] The sugarcane root-promoting and seedling-promoting medium of this embodiment is prepared by including mixture I containing macroelements, mixture II containing trace elements, iron salt mixture, organic mixture, plant hormones and sucrose, and using agar as a solidifying agent.

[0049] Mixture I with a large number of elements including KNO 3 1000 mg / L, Ca(NO 3 ) 2 4H 2 O 800 mg / L, NH 4 NO 3 500 mg / L, KH 2 PO 4 150 mg / L, MgSO 4 ·7H 2 O 200 mg / L and CaCl 2 2H 2 O 80 mg / L, the molar concentration ratio of nitrate nitrogen and ammonium nitrogen is 3.67:1, and the total nitrogen content is 29.2mmol / L.

[0050] Mixture II with trace elements includes H 3 BO 3 4 mg / L, MnSO 4 4H 2 O 15 mg / L, ZnSO 4 ·7H 2 O 6 mg / L, NaMoO 4 2H 2 O 0.2 mg / L, KI 0.5 mg / L.

[0051] The iron salt mixture includes Na 2 -EDTA 22 mg / L, FeSO 4 ·7H 2 O 16 mg / L and Na 2 SiO 3 9H 2 O 33mg / L.

[0052] The organic mixture included inositol 65 mg / L, glycine 0.6 mg / L, thiamine h...

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