A carbon-nitrogen fluorescent quantum dot tumor cell detection kit and a method of using the same

By using a tumor cell detection kit prepared with carbon-nitrogen fluorescent quantum dots, the problems of high false negative rate, low accuracy and operational complexity in tumor cell detection have been solved, achieving high sensitivity and low cost in tumor cell detection, thus improving diagnostic level and support for personalized treatment.

CN115184313BActive Publication Date: 2025-11-25SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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Patent Information

Application Number
CN202111227034.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2020-10-27
Filing Date
2021-10-21
Publication Date
2025-11-25
Estimated Expiration
2041-10-21

AI Technical Summary

Technical Problem

Existing technologies for tumor cell detection suffer from problems such as high false negative rates, low accuracy and sensitivity, complex operation, and high cost, especially in the detection of tumor cells in pleural effusion and urine.

Method used

A tumor cell detection kit prepared using carbon-nitrogen fluorescent quantum dots includes solution A and solution B. Solution A is a carbon-nitrogen fluorescent quantum dot solution, and solution B is a buffer solution. Specific operating procedures are used to process pleural fluid or urine samples to improve the specificity and sensitivity of the detection.

Benefits of technology

It improves the accuracy and reproducibility of tumor cell detection, reduces operational complexity and cost, and provides support for higher diagnostic levels and personalized treatment.

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Abstract

The application discloses a kind of carbon-nitrogen fluorescent quantum dot tumor cell detection kit and its use method, it is characterized in that, including A liquid and B liquid;Wherein, the A liquid is carbon-nitrogen fluorescent quantum dot solution, the B liquid is buffer solution;The carbon-nitrogen fluorescent quantum dot solution is composed of carbon-nitrogen fluorescent quantum dot and solvent.Its use process, at least includes the following steps: step S10, provides reagent kit A liquid, B liquid, patient pleural fluid, urine sample;Step S20, high-speed centrifugation, after B liquid resuspension, add A liquid dyeing;Step S30, high-speed centrifugation, B liquid resuspension;Step S40, spectrophotometer detects fluorescence intensity.The detection of tumor cells in pleural fluid and urine tissue fluid of the application has high specificity and high sensitivity, and simple operation, cost is small.Not only can improve the diagnosis level of doctor to clinical tumor patient, also can provide help for prognosis evaluation of tumor, drug screening, individualized treatment.
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