Cultivation technology of promoting lactic acid bacteria proliferation using red ginseng

Abstract

The present invention provides: a lactic acid bacteria culture medium comprising dietary fiber derived from red ginseng, and a method for culturing lactic acid bacteria using the culture medium. According to the present invention, the dietary fiber derived from red ginseng is used in the lactic acid bacteria culture medium, so that the number of viable cells of lactic acid bacteria can be increased and the culturability can be improved. In addition, the method for culturing lactic acid bacteria of the present invention uses the culture medium, so that the culturability is improved under the same conditions, and therefore there is an advantage in terms of cost.

Description

Technical Field

[0001] The present invention relates to a culture medium for lactic acid bacteria containing red ginseng raw material and a method for culturing lactic acid bacteria using the culture medium. Background Art

[0002] Ginseng (Panax ginseng CAMeyer), as a perennial herb of the genus Panax in the Araliaceae family, is one of the herbs used in oriental medicine. It has a sweet and bitter taste, warming and tonic energy, and has herbal effects such as strengthening vitality, protecting the spleen, and strengthening the lungs.

[0003] The main physiologically active substances of ginseng include saponin (saponin) (also referred to as ginsenoside), essential oil components, polyacetylene, phenol components, glycosides, acidic peptides, etc., and include other various components such as vitamins, sugars, and minerals. It is reported that the physiologically active efficacy of ordinary ginseng has the effect on the central nervous system, the effect of enhancing immune function, anticancer effect, etc. In particular, to date, about 40 types of ginsenosides have been found, and it has been confirmed that it shows excellent effects on body function regulation, i.e., normalization of physiological functions, by widely affecting the endocrine system, immune system, metabolic system, etc. including the central nervous system. These ginsenosides have similar or opposite effects, and are known to show various effects by specific ingredients alone or by the interaction of several types thereof.

[0004] On the other hand, dietary fiber is a component known as a large amount of fiber or cellulose contained in vegetables, fruits, seaweed, etc. in food, and is a high-molecular carbohydrate that is not digested by human digestive enzymes and excreted from the body. Known fiber is a beneficial ingredient in the diet by making the stomach feel less hungry and delaying the absorption of food to filter cholesterol. These fibers are rich in the tough part of vegetables (cellulose), the brown pigment (lignin) of radish, pectin in fruits, and the sticky components (alginic acid) of sea mustard and kelp, especially mushrooms.

[0005] Lactic acid bacteria is the general name of the bacterium that produces a large amount of lactic acid as metabolites, and it is known that it has physiological function by keeping the constant ratio of various enteric microorganisms in the intestine and promoting intestinal movement, for example improving constipation, improving immunity etc. Because lactic acid bacteria is susceptible to the influence of temperature, acidity, pressure etc. usually, depends on flow process or storage environment, the amount of lactic acid bacteria may not reach the recommended content of the Ministry of Food and Drug Safety. Therefore, in order to improve the stability of lactic acid bacteria, the manufacture method of lactic acid bacteria has been carried out to lasting research (patent documentation 1).At this, in the cultivation of lactic acid bacteria, because the viable cell number of lactic acid bacteria has very large difference according to culture condition, therefore can improve the research of the condition of the culturability of lactic acid bacteria also continue always.

[0006] The present inventors conducted various studies using red ginseng, and confirmed that when red ginseng-derived dietary fiber from which water-soluble components of red ginseng were removed was used in a culture medium for lactic acid bacteria, the number of viable cells of lactic acid bacteria increased and culturability was significantly improved, thereby completing the present invention.

[0007] [Prior art literature]

[0008] [Patent Document]

[0009] (Patent Document 1) Korean Patent Publication No. 10-2048690 Summary of the invention

[0010]

Technical issues

[0011] An object of the present invention is to provide a culture medium for lactic acid bacteria having improved culturability of lactic acid bacteria and a method for culturing lactic acid bacteria using the culture medium.

[0012]

Technical solution

[0013] One aspect of the present invention provides a culture medium for lactic acid bacteria, comprising dietary fiber derived from red ginseng.

[0014] Another aspect of the present invention provides a method for culturing lactic acid bacteria, the method comprising culturing lactic acid bacteria in a lactic acid bacteria culture medium containing red ginseng-derived dietary fiber.

[0015]

Beneficial Effects

[0016] According to the present invention, by using the dietary fiber derived from red ginseng in the culture medium for lactic acid bacteria, the number of viable cells of lactic acid bacteria increases, thereby improving culturability. In addition, according to the method for culturing lactic acid bacteria of the present invention, since culturability is improved by using the culture medium under the same conditions, there is an advantage in cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The results of culturing Bifidobacterium lactis in Examples and Comparative Examples are shown.

[0018] Figure 2 The results of culturing Bifidobacterium breve in Examples and Comparative Examples are shown. DETAILED DESCRIPTION

[0019] Hereinafter, the present invention will be described in more detail to help understanding of the present invention.

[0020] Based on the principle that inventors can appropriately define the concepts of terms in order to describe their own inventions in the best manner, the terms and words used in this specification and claims should not be interpreted as limited to typical or dictionary meanings, but should be interpreted as having meanings and concepts consistent with the technical spirit of the present disclosure.

[0021] 1. Culture medium for lactic acid bacteria

[0022] One aspect of the present invention provides a culture medium for lactic acid bacteria, comprising dietary fiber derived from red ginseng.

[0023] In the present invention, "red ginseng" refers to steamed ginseng (Panaxginseng). Specifically, red ginseng can be prepared by steaming the root of ginseng with steam and then drying the steamed root, and any material prepared using ginseng as a raw material by a conventional preparation method used in the art can be used in the present invention. Red ginseng can be slightly reddish when prepared by the same method as above, and can be concentrated and contain ginseng ingredients. The active ingredients contained in red ginseng, such as saponins, may be different in amount and type from the active ingredients contained in ginseng, and red ginseng has less bitterness and stronger sweetness or better texture than ginseng and is easier to store. Ginseng can be used without restriction, such as cultivated or commercially available ginseng.

[0024] In the present invention, "dietary fiber" refers to high-molecular carbohydrates that are not digested by human digestive enzymes but are excreted from the body, and may include, for example, pectin, gum, cellulose, lignin, and the like.

[0025] In the present invention, "red ginseng-derived dietary fiber" refers to dietary fiber obtained from red ginseng, and its obtaining method is not limited. Specifically, the red ginseng-derived dietary fiber can be obtained and prepared from red ginseng by conventional methods, and can be obtained from red ginseng raw materials, red ginseng extracts, or residues generated during the extraction process of red ginseng. For example, the red ginseng-derived dietary fiber can be extracted and obtained from the residues in the process of extracting red ginseng, but when the dietary fiber is obtained from the residues generated during the preparation of red ginseng extracts for various purposes, it is advantageous in terms of more efficiency and economy.

[0026] The dietary fiber derived from red ginseng can be extracted from the residue in the extraction process of red ginseng using a centrifuge, a filter press, a belt press, a dryer, etc., but is not particularly limited thereto.

[0027] When the red ginseng-derived dietary fiber is extracted from the residue using a dryer, the drying process using the dryer may be performed at 80° C. or higher, 90° C. or higher, or 100° C. to 200° C., but is not particularly limited thereto.

[0028] The red ginseng extraction process can be performed using all conventionally known extraction methods, such as solvent extraction, ultrasonic extraction, filtration, and reflux extraction, and, for example, solvent extraction or reflux extraction can be used. The extraction process can be repeated several times.

[0029] The red ginseng extraction process can be performed using water, an organic solvent, a supercritical fluid, a mixture thereof, etc. as an extraction solvent. The organic solvent can be an alcohol, preferably a C1-C4 lower alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, a mixed solvent, etc., for example, ethanol. For example, when a mixture of water and an organic solvent is used as an extraction solvent, the mixture of water and the organic solvent can be specifically a mixture of water and a C1-C4 lower alcohol, more specifically a mixture of water and ethanol.

[0030] The red ginseng extraction process may be performed at 0°C to 100°C, specifically 40°C to 90°C, more specifically 80°C to 90°C, but not limited thereto. The red ginseng extraction process may be performed for 6 hours to 36 hours, specifically 7 hours to 24 hours, more specifically 8 hours to 14 hours, but not limited thereto. The extraction may be performed 1 to 8 times, specifically 1 to 6 times, and more specifically 1 to 5 times, but not limited thereto.

[0031] In addition, the dietary fiber derived from red ginseng may contain ginsenosides such as Rb1, Rg3, Rg1, and Re, red ginseng polysaccharides, and other components in addition to fiber. Therefore, the dietary fiber derived from red ginseng of the present invention can exert various physiologically active functions through ginsenosides, red ginseng polysaccharides, etc., and can also exert functions through ordinary fibers. In the present invention, the dietary fiber derived from red ginseng is used as a culture medium for lactic acid bacteria, so it has good culturability.

[0032] The culture medium for lactic acid bacteria of the present invention may contain 0.001% (w / v) to 5% (w / v) of dietary fiber derived from red ginseng. Specifically, the dietary fiber derived from red ginseng may be contained in an amount of 0.005% (w / v) to 4.5% (w / v), 0.01% (w / v) to 4%, 0.1% (w / v) to 3.5%, or 1% to 3%, but is not limited thereto. Since the culture medium for lactic acid bacteria of the present invention contains dietary fiber derived from red ginseng in the above-mentioned content range, the effect of increasing the number of viable cells by promoting the growth of lactic acid bacteria cultured in the culture medium is very good. In particular, even if the content of dietary fiber derived from red ginseng is relatively low within the above-mentioned range, the effect of promoting the proliferation of lactic acid bacteria is excellent. Therefore, according to the purpose of cultivation, the content of dietary fiber can be appropriately selected and adjusted for use.

[0033] In the present invention, "lactic acid bacteria" is a general term for bacteria that produce a large amount of lactic acid as a metabolite, and may include, for example, Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Leuconostoc, Pediococcus, Bifidobacterium, and the like.

[0034] As an example, the lactic acid bacteria may include Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus delbrueckii subsp. lactis, Lactococcus lactis, Lactococcus lactis cremoris, Lactococcus lactis subsp. diacetyl, Lactococcus lactis subsp. lactis, Enterococcus faecium, Enterococcus faecalis, Streptococcus thermophilus, Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium animalis subsp. lactis, Bifidobacterium infantis, Bifidobacterium lactis, Bacillus coagulans, etc., but are not limited to the above-mentioned lactic acid bacteria, and include all lactic acid bacteria that can be used in food.

[0035] The culture medium of the present invention is not particularly limited as long as it is a culture medium commonly used for culturing lactic acid bacteria, and for example, MRS, BL, M17, NB, BHI culture medium, etc. can be used. Since the culture medium of the present invention contains dietary fiber derived from red ginseng and components contained in a culture medium commonly used for culturing lactic acid bacteria, the number of viable cells of lactic acid bacteria cultured using the culture medium can be significantly increased, thereby promoting the growth of lactic acid bacteria and improving culturability.

[0036] The culture medium of the present invention may further contain one or more selected from the group consisting of sugars, nitrogen sources, trace elements and carbonates as other components required for culturing lactic acid bacteria.

[0037] The carbohydrate component can be a mixture of lactose, fructose, sucrose, glucose, maltose, trehalose and the like.

[0038] The nitrogen source component can be yeast extract, soy peptone, casein peptone and the like.

[0039] Trace elements may include potassium diphosphate, magnesium sulfate, manganese sulfate, calcium chloride, potassium citrate, sodium acetate, L-ascorbic acid, L-glutamic acid, L-cysteine ​​hydrochloride, polysorbate 80, and the like.

[0040] The carbonate may be potassium carbonate, calcium carbonate, sodium carbonate or the like.

[0041] 2. Cultivation method of lactic acid bacteria

[0042] Another aspect of the present invention provides a method for culturing lactic acid bacteria using a culture medium for lactic acid bacteria.

[0043] The method for culturing lactic acid bacteria comprises culturing lactic acid bacteria in a culture medium containing dietary fiber derived from red ginseng.

[0044] As for the "culture medium", since the same contents as described above can be applied, the detailed description will be omitted.

[0045] First, lactic acid bacteria are cultured in a culture medium.

[0046] Inoculation can be done by adding 10 7 CFU / mL to 10 11 CFU / mL, 5×10 7 CFU / mL to 10 10 CFU / mL or 10 8 CFU / mL to 10 9 The method is performed by inoculating lactic acid bacteria at a concentration of 100 CFU / mL, but is not limited thereto, and the concentration of lactic acid bacteria to be inoculated can be appropriately selected according to the cultivation purpose of lactic acid bacteria, cultivation time, etc.

[0047] Then, lactic acid bacteria are cultured.

[0048] The culturing step may be based on conventional conditions generally used in the art for culturing lactic acid bacteria, and is not particularly limited.

[0049] For example, the culture temperature condition can be a temperature of 30°C to 40°C, 35°C to 40°C, or 35°C to 37°C. The lactic acid bacteria can be cultured under a culture relative humidity condition, and the relative humidity condition is 65% to 85%, 70% to 80%, or 72% to 78%. The culture step can be under anaerobic conditions, for example, under conditions where nitrogen or carbon dioxide gas replaces air, to culture the lactic acid bacteria. The culture time for culturing the lactic acid bacteria can be 6 to 48 hours, 10 to 40 hours, or 15 to 24 hours.

[0050] When culturing lactic acid bacteria by the culturing method of the present invention, the proliferation of lactic acid bacteria is promoted, and the viable cell count of lactic acid bacteria is increased rapidly. That is, compared with the case of culturing lactic acid bacteria in a conventional lactic acid bacteria culture medium, the case of culturing lactic acid bacteria by the culturing method of the present invention has the advantage of increasing the viable cell count by 16% to 59%.

[0051] Hereinafter, the present invention will be described in detail by way of Examples and Comparative Examples.

[0052] However, the following preparation examples and experimental examples are merely for illustration of the present invention, and the contents of the present invention are not limited to the following preparation examples and experimental examples.

[0053] Example 1

[0054] Red ginseng was added to purified water and extracted at 87°C for 24 hours to separate the red ginseng extract and the residue. In order to obtain red ginseng dietary fiber (DF), water was removed from the red ginseng residue except the red ginseng extract through a dehydration process, and the residue was dried using a dryer at 110°C to obtain red ginseng dietary fiber (DF).

[0055] The culture medium of the present invention is prepared by adding red ginseng dietary fiber to MRS culture medium generally used for culturing lactic acid bacteria. Specifically, a culture medium (Example 1-1) with 1% (w / v) of red ginseng dietary fiber added to the total culture medium, a culture medium (Example 1-2) with 2% (w / v) of red ginseng dietary fiber added to the total culture medium, and a culture medium (Example 1-3) with 3% (w / v) of red ginseng dietary fiber added to the total culture medium were prepared and then used for lactic acid bacteria culture experiments.

[0056] Comparative Examples 1 and 2

[0057] The culture media of Comparative Examples 1 and 2 were prepared by adding other ingredients from red ginseng to a conventional MRS culture medium without adding the red ginseng-derived dietary fiber of Example 1. Specifically, the culture media of Comparative Examples 1-1 to 1-3 were prepared by adding a "red ginseng source liquid (CW)" obtained by collecting a steamed ginseng effluent produced during ginseng steaming and then sterilizing it to the MRS culture medium at concentrations of 1, 5, and 10% (w / v), respectively. The culture media of Comparative Examples 2-1 to 2-3 were prepared by adding a "red ginseng source concentrate (CCW)" concentrated by centrifuging a steamed ginseng effluent to remove starch to the MRS culture medium at concentrations of 1, 2, and 3% (w / v), respectively.

[0058] Experimental example

[0059] Lactic acid bacteria were inoculated and cultured in the culture medium of the examples and comparative examples, and the number of viable cells was determined on an MRS agar plate. Bifidobacterium lactis IDCC 4301 strain (LA4301) and Bifidobacterium breve IDCC 4401 strain (BR 4401) were used as lactic acid bacteria. Each lactic acid strain was cultured to 5×10 9 CFU / ml, inoculated in the culture medium of the embodiment and the comparative example at 5% (v / v), cultured at 37°C, 75% relative humidity, anaerobic conditions for 40 hours, and the change in the number of viable cells was measured. In the control group, the change in the number of viable cells was measured using an untreated group without adding red ginseng dietary fiber, and the change rate of the number of viable cells compared with the control group was calculated as a percentage. The results are shown in Table 1 below.

[0060] [Table 1]

[0061]

[0062] As a result, in the culture medium of Comparative Examples 1-1 to 1-3 and Comparative Examples 2-1 to 2-3 containing red ginseng source liquid or red ginseng source concentrate, the number of live cells of lactic acid bacteria did not increase or decrease significantly compared with the control group. That is, when culturing Bifidobacterium lactis, the more the content of red ginseng source liquid or red ginseng source concentrate in the comparative example increases, the more the number of live cells tends to increase, but the value is not large. In the culture medium of Comparative Example 2-3 containing 3% (w / v) red ginseng source concentrate, the number of live cells decreased by 15%, and the effect of promoting lactic acid bacteria culture was not significantly manifested. In addition, it was confirmed that when culturing Bifidobacterium breve, in the culture medium of the comparative example, the number of live cells decreased, which was not suitable for the culture of lactic acid bacteria.

[0063] On the other hand, in the culture medium of Examples 1-1 to 1-3 containing the red ginseng dietary fiber of the present invention, the number of viable cells of lactic acid bacteria increased significantly regardless of whether Bifidobacterium lactis or Bifidobacterium breve was cultured. That is, the increase rate of the number of viable cells was measured to be more than 16% and less than 59%, and when lactic acid bacteria were cultured in the culture medium of the present invention, it was confirmed that its culturability was greatly improved. In addition, even when a culture medium containing a small amount of 1% (w / v) of red ginseng dietary fiber (Example 1-1) was used, the increase rate of the number of viable cells was high. Therefore, according to the purpose of cultivation, suitable culture conditions can be set in various ways, and excellent effects can be obtained even with a small amount of red ginseng dietary fiber, so it can be expected that there is an advantage even in terms of cost.

Claims

1. Use of a culture medium comprising dietary fiber derived from red ginseng in promoting the proliferation of lactic acid bacteria, The red ginseng is extracted with water, the red ginseng extract and the residue are separated, and the residue is dehydrated to obtain the dietary fiber derived from the red ginseng, and The lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus reuteri, Bifidobacterium breve, and Bifidobacterium lactis.

2. The use according to claim 1, wherein the dietary fiber derived from red ginseng is extracted from residues in a red ginseng extraction process using at least one selected from the group consisting of a centrifuge, a filter press, a belt press and a dryer.

3. A method for promoting the proliferation of lactic acid bacteria, comprising inoculating and culturing lactic acid bacteria in a lactic acid bacteria culture medium containing dietary fiber derived from red ginseng, The red ginseng is extracted with water, the red ginseng extract and the residue are separated, and the residue is dehydrated to obtain the dietary fiber derived from the red ginseng, and The lactic acid bacteria is at least one selected from the group consisting of Lactobacillus plantarum, Lactobacillus reuteri, Bifidobacterium breve, and Bifidobacterium lactis.

4. The method for promoting the growth of lactic acid bacteria according to claim 3, wherein: The culturing is carried out at a temperature of 35°C to 40°C.

5. The method for promoting the growth of lactic acid bacteria according to claim 3, wherein: The cultivation was carried out under anaerobic conditions.

6. The method for promoting the growth of lactic acid bacteria according to claim 3, wherein: The culturing is performed for 6 to 48 hours.

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