A dry analytical reagent for total cholesterol detection

By designing a dry analytical reagent with multi-layer structures, using polystyrene microspheres and soap-free emulsion polymerization technology, environmental pollution and detection accuracy problems are solved, and efficient and accurate total cholesterol detection is achieved.

CN115436615BActive Publication Date: 2025-09-02LUCKY HEALTHCARE CO LTD
5 Cites 0 Cited by

Patent Information

Application Number
CN202211010692.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-08-23
Publication Date
2025-09-02
Estimated Expiration
2042-08-23

AI Technical Summary

Technical Problem

In total cholesterol detection, existing dry analytical reagents have problems such as complex production processes, the use of organic solvents leads to environmental pollution, and the inability to achieve high-precision quantitative analysis.

Method used

The upper support layer, diffusion layer, reagent layer, support and lower support layer structure are adopted, which are arranged from top to bottom. The diffusion layer is composed of polystyrene microspheres and adhesive to form a porous diffusion film with high porosity. The reagent layer contains specific enzymes and dyes, avoiding the use of organic solvents, and polystyrene microspheres are prepared through soap-free emulsion polymerization technology to form a three-dimensional lattice structure.

Benefits of technology

It realizes an environmentally friendly production process, fast and accurate total cholesterol detection, and is suitable for immediate and on-site diagnosis, with high accuracy in detection results and wide linear range.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

A dry analytical reagent for total cholesterol detection comprises, from top to bottom, an upper supporting layer, a diffusion layer, a reagent layer, a support, and a lower supporting layer, wherein the diffusion layer, the reagent layer, and the support are sequentially arranged in a middle position between the upper supporting layer and the lower supporting layer, the pores at both ends of the upper supporting layer and the lower supporting layer are bonded by the middle supporting layer, and a sample addition hole and a test hole are respectively arranged in the middle position of the upper supporting layer and the lower supporting layer, the diffusion layer is an isotropic porous diffusion membrane with a porosity of 50-85%; the diffusion layer is composed of polystyrene microspheres, an adhesive, and a surfactant, and the particle size of the polystyrene microspheres is 0.1-1 μm; the reagent layer includes a surfactant, cholesterol esterase, cholesterol oxidase, horseradish peroxidase, a colorless dye, a buffer, a stabilizer, and a water-soluble high molecular polymer. The diffusion layer prepared by the present invention has a diffusion path of tens to hundreds of nanometers, which can quickly absorb, evenly distribute, measure, and quickly transmit multiple analytes.
Need to check novelty before this filing date? Find Prior Art