A pigeon meat antioxidant peptide and an anti-fatigue peptide and a preparation method thereof

The preparation of antioxidant peptides and anti-fatigue peptides from fresh pigeon breasts through enzymatic methods has solved the problems of high preparation costs and many by-products in the prior art, and achieved efficient and low-cost peptide preparation, and the product has significant antioxidant and anti-fatigue activities.

CN115786434BActive Publication Date: 2025-06-03ZHONGKAI UNIV OF AGRI & ENG +1
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Patent Information

Application Number
CN202211619327.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-14
Publication Date
2025-06-03
Estimated Expiration
2042-12-14

AI Technical Summary

Technical Problem

In the prior art, when preparing antioxidant peptides and anti-fatigue peptides, there are problems of high cost and many by-products, especially when using cost-effective protease enzymatic method, the process conditions are complex and the product complexity is high.

Method used

Fresh pigeon breast meat was used as raw material, and pigeon meat antioxidant peptide was prepared by enzymatic method, and the pigeon meat anti-fatigue peptide was isolated and purified by ultrafiltration. The method includes enzymatic decomposition of pigeon meat with neutral and alkaline protease complexes, and subsequently obtaining antioxidant peptides by centrifugation and lyophilization, and isolation of anti-fatigue peptides through ultrafiltration membranes.

Benefits of technology

The prepared pigeon meat antioxidant peptide and anti-fatigue peptide have significant antioxidant activity and anti-fatigue effect, and the molecular weight is less than 5000 Da, which is easy to be absorbed by the human body, significantly improving SOD enzyme activity.

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Abstract

The present invention belongs to the field of functional peptides, and specifically discloses a pigeon meat antioxidant peptide and an anti-fatigue peptide and a preparation method thereof, comprising the following steps: (1) Take pigeon meat, mince it, add water and a compound enzyme of neutral protease and alkaline protease, carry out constant-temperature enzymatic hydrolysis, centrifuge to obtain a supernatant, and lyophilize to obtain the pigeon meat antioxidant peptide; (2) The supernatant in step (1) is intercepted by an ultrafiltration membrane separation method to obtain a fraction of 1-5 kDa, and lyophilized to obtain the pigeon meat anti-fatigue peptide. The antioxidant peptide of the present invention has relatively high contents of essential amino acids and antioxidant active amino acids, reaching 47.97% and 29.27% respectively. The anti-fatigue peptide of the present invention is a peptide with a molecular weight lower than 5000 Da, which is relatively easy to be absorbed by the human body, has the effect of significantly improving the enzyme activity of SOD enzyme, and has a significant anti-fatigue effect.
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