Method for studying whole surface mechanics of a single adherent cell under physiological conditions

By combining optical microscopy and nanoindentation technology with grid setting and algorithm processing, high-resolution imaging of the full surface mechanical spectrum of a single adherent cell was achieved, which solves the limitations and randomness problems of full surface testing in existing technologies and provides a reliable theoretical basis.

CN115993315BActive Publication Date: 2025-12-09CHANGCHUN UNIV OF SCI & TECH
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Patent Information

Application Number
CN202111200789.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-10-18
Publication Date
2025-12-09
Estimated Expiration
2041-10-18

AI Technical Summary

Technical Problem

Existing technologies for testing cell mechanical properties under physiological conditions are limited to single-point testing, making it difficult to achieve high-resolution imaging of the entire surface mechanical spectrum, and may lead to cell damage or randomness in experimental results.

Method used

Using optical microscopes, micromanipulation systems, nanoindentation technology, grid settings, point-by-point mechanical property testing, adhesion extraction algorithms, and pixel brightness settings, combined with nanoindentation technology, point-by-point testing of the cell surface is performed to generate a grid pixel mechanical map.

Benefits of technology

This study achieved high-resolution mechanical mapping of the entire surface of a single adherent cell under physiological conditions, improving the timeliness of experiments, avoiding the randomness of mechanical information, and providing a reliable theoretical basis.

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Abstract

The application discloses a method for researching a whole surface mechanical atlas of a single adherent biological cell under physiological conditions, and belongs to the technical field of microscopic imaging testing. The method is based on the principle of nanoindentation technology under physiological conditions, and uses an optical microscope to perform coarse positioning on a sample to be tested, and selects the sample and sets a grid standard according to requirements. Each square of the grid is used as a basic unit, and mechanical property testing is performed on the position of each center point, and finally the testing results are output in the form of a grid pixel, so that the whole surface mechanical atlas of the adherent cell is obtained. The purpose of the application is to obtain the mechanical information of the whole cell surface under physiological conditions by using microscopic manipulation technology, and to output the analysis results in the mode of a pixel map, so that people can more intuitively understand the adhesion force difference of the whole cell surface, and a method for testing results is provided, which is more intuitive, simple and comprehensive for the in-depth research on the physical properties of cells.
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