A proteinase adsorption circulating box and a circulating adsorption method thereof

Abstract

This invention discloses a protease adsorption circulating chamber and its circulating adsorption method. The bottom of the circulating chamber is connected to the inlet end. A first automatic feeding device and a second automatic feeding device are provided above the circulating chamber. The first inner cavity of the circulating chamber is connected to the pump inlet of a first pump body and the pump outlet of a second pump body. The pump outlet of the first pump body and the pump inlet of the second pump body are connected to the inlet end of a first three-way valve. The first outlet end of the first three-way valve is connected to the bottom of the fourth inner cavity of the circulating chamber. The second outlet end of the first three-way valve is connected to the second outlet end of a second three-way valve. The inlet end of the second three-way valve is connected to the bottom of the third inner cavity of the circulating chamber. The circulating chamber is provided with a first circulating branch pipe and a second circulating branch pipe. The bottom of the second inner cavity of the circulating chamber and the first outlet end of the second three-way valve are both connected to the collection end. This invention can simultaneously complete the circulating adsorption of two proteases, and can customize the increase or decrease of the types of proteases collected, and specifically control the processing volume.

Description

Technical Field

[0001] This invention relates to the field of protease extraction equipment technology, and in particular to a protease adsorption circulation box and its circulation adsorption method. Background Technology

[0002] Male urine contains proteases such as urokinase and ulinastatin. A common method for extracting proteases from urine is the adsorption method. Taking urokinase as an example, the specific process flow is as follows:

[0003] S1. Collect male urine through public toilets in schools, factories, etc. The freshness of the urine will affect the activity of urokinase, so it must be processed in time. Collect and process the urine from the toilet at regular intervals every day, adjust the pH value of the urine to 9, let it stand for 1 hour, siphon the supernatant and discard the grayish-white precipitate.

[0004] S2. Adsorption: Adjust the pH of the siphon supernatant to 5.8-6 with 5N hydrochloric acid (HCl), add 1% (WIV) resin, stir and adsorb for 1 hour, stop stirring, allow the resin to settle naturally, siphon off the residual urine, and collect the resin. Wash the collected resin three times with tap water, then wash it three more times with deionized water until the urine is colorless;

[0005] S3, Elution: For the first elution, use 2% ammonium hydroxide and 1M sodium chloride eluent, stir and elute for 1 hour at 35% volume; for the second elution, use the same eluent and the same volume for 30 minutes, and then start collecting urokinase.

[0006] S4. After the finished urokinase is sent to a testing unit to test its activity, it can be sold to crude product purchasers or biochemical pharmaceutical companies.

[0007] In existing processes for extracting proteases from urine, most steps are performed manually, especially protease adsorption, which is inefficient. Furthermore, in the adsorption processes of some existing extraction devices, the adsorbent material tends to settle at the bottom of the urine, limiting its adsorption capacity for proteases. In addition, current extraction equipment cannot simultaneously adsorb and extract two types of proteases, nor does it consider adapting the processing volume to the actual urine collection volume. Summary of the Invention

[0008] The purpose of this invention is to provide a protease adsorption cycle box and its cycle adsorption method in view of the existing technology. It can simultaneously complete the cycle adsorption of two proteases, and can customize the increase or decrease of the types of proteases collected, and specifically control the processing volume.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] A protease adsorption circulation box includes a circulation box, a first pump body and a second pump body, a first three-way valve and a second three-way valve;

[0011] The inner cavity of the circulation box is provided with a first slot, a second slot, a third slot, a fourth slot, a fifth slot, a sixth slot, and a seventh slot from left to right. Partitions are inserted into the second, fourth, and sixth slots to sequentially divide the inner cavity of the circulation box into a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity; or partitions are inserted into the first, third, fourth, fifth, and seventh slots to sequentially divide the inner cavity of the circulation box into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity, and a second empty cavity; or partitions are inserted into the first, third, fourth, and fifth slots to sequentially divide the inner cavity of the circulation box into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity.

[0012] The bottom of the first inner cavity of the circulation tank is connected to the inlet end through an inlet pipe, and a first electric valve is provided on the inlet pipe. The bottom of the first inner cavity of the circulation tank is connected to the outlet end through a drain pipe, and a second electric valve is provided on the drain pipe. A first automatic feeding device and a second automatic feeding device are provided above the circulation tank, which are used to feed different adsorbent materials into the second inner cavity and the third inner cavity of the circulation tank, respectively. A first liquid level sensor is provided on the circulation tank to monitor the liquid level in the circulation tank.

[0013] The first inner cavity of the circulation tank is connected to the pump inlet of the first pump body and the pump outlet of the second pump body. The pump outlet of the first pump body and the pump inlet of the second pump body are connected to the inlet end of the first three-way valve. The first outlet end of the first three-way valve is connected to the bottom of the fourth inner cavity of the circulation tank. The second outlet end of the first three-way valve is connected to the second outlet end of the second three-way valve. The inlet end of the second three-way valve is connected to the bottom of the third inner cavity of the circulation tank. The circulation tank is provided with a first circulation branch pipe connected to its second inner cavity. A spray head is provided at one end of the first circulation branch pipe located in the second inner cavity. The other end is connected to the pump outlet of the second pump body and is equipped with a third electric valve. The circulation box is equipped with a second circulation branch pipe that connects to its third inner cavity. One end of the second circulation branch pipe located in the third inner cavity is equipped with a spray head. The other end of the second circulation branch pipe is connected to the first outlet end of the first three-way valve and is equipped with a fourth electric valve. The bottom of the second inner cavity of the circulation box is connected to the collection end through the first collection branch pipe and is equipped with a fifth electric valve. The first outlet end of the second three-way valve is connected to the collection end through the second collection branch pipe and is equipped with a sixth electric valve.

[0014] When collecting two proteases using two adsorbent materials, the partitions inserted in the second, fourth, and sixth slots of the circulation chamber are all sieve plates, or the partitions inserted in the first and seventh slots are all solid plates, and the partitions inserted in the third, fourth, and fifth slots are all sieve plates. In addition, the inlet end of the first three-way valve is connected to the first outlet end, and the inlet end of the second three-way valve is connected to the first outlet end.

[0015] When collecting a protease using an adsorbent material, the partitions inserted in the second and fourth slots of the circulation chamber are screen plates, and the partition inserted in the sixth slot is a solid plate; or the partitions inserted in the first and fifth slots are solid plates, and the partitions inserted in the third and fourth slots are screen plates. The inlet end of the first three-way valve is connected to the second outlet end, and the inlet end of the second three-way valve is connected to the second outlet end.

[0016] Furthermore, the first, second, third, and fourth inner cavities of the circulation tank are all connected to cleaning branch pipes, each of which is equipped with a seventh electric valve, and each has a spray head at one end inside the circulation tank and is connected to a water source at the other end.

[0017] Furthermore, the spray heads installed on the cleaning branch pipes connecting the first, second, third, and fourth inner cavities of the circulation box are all located at the top of the corresponding inner cavity in the circulation box.

[0018] Furthermore, the spray head installed on the first circulation branch pipe is located at the bottom of the second inner cavity of the circulation box, and the spray head installed on the second circulation branch pipe is located at the bottom of the third inner cavity of the circulation box.

[0019] Furthermore, the circulation tank is connected to the discharge end via an overflow pipe.

[0020] Furthermore, filters are provided on both the pump inlet side of the first pump body and the pump inlet side of the second pump body.

[0021] Furthermore, partitions are inserted into the second, fourth, and sixth slots to divide the area inside the circulation box into equal parts; partitions are inserted into the first, third, fourth, fifth, and seventh slots to divide the area between the first and seventh slots inside the circulation box into equal parts; partitions are inserted into the first, third, fourth, and fifth slots to divide the area between the first and fifth slots inside the circulation box into equal parts.

[0022] A method for cyclic adsorption of a protease adsorption box, comprising the following steps when collecting two proteases using two adsorbent materials: Urine is injected into the circulation box via an inlet pipe; subsequently, one adsorbent material is added to the second inner cavity of the circulation box via a first automatic feeding device, and another adsorbent material is added to the third inner cavity of the circulation box via a second automatic feeding device. Simultaneously, the following two steps are performed alternately at a first preset time: urine from the first inner cavity of the circulation box is injected into its third and fourth inner cavities via a first pump, and urine from the fourth inner cavity of the circulation box is injected into its first and second inner cavities via a second pump; finally, the mixture of urine and corresponding adsorbent material in the second inner cavity of the circulation box is discharged to the collection end via a first collection branch pipe, and the mixture of urine and corresponding adsorbent material in the second inner cavity of the circulation box is discharged to the collection end via a second collection branch pipe.

[0023] When collecting a protease using an adsorbent material, the following steps are performed: urine is injected into a circulation tank through an inlet pipe; then, an adsorbent material is added to the second inner cavity of the circulation tank through a first automatic feeding device. Simultaneously, the following two steps are performed alternately at a second preset time: urine from the first inner cavity of the circulation tank is injected into its third inner cavity through a first pump, and urine from the third inner cavity of the circulation tank is injected into its first and second inner cavities through a second pump; finally, the mixture of urine and the corresponding adsorbent material in the second inner cavity of the circulation tank is discharged to the collection end through a first collection branch pipe.

[0024] Furthermore, when collecting two proteases using two adsorbent materials, the method further includes the following steps: discharging the mixture of urine and corresponding adsorbent materials in the second inner cavity of the circulation chamber to the collection end through the first collection branch pipe, and discharging the mixture of urine and corresponding adsorbent materials in the third inner cavity of the circulation chamber to the collection end through the second collection branch pipe, and then pumping cleaning water to the corresponding nozzles through the cleaning branch pipes in the first, second, third and fourth inner cavities of the circulation chamber to rinse the adsorbent materials remaining on the screen plate;

[0025] When collecting a protease using an adsorbent material, the method further includes the following steps: after the urine mixture in the second inner cavity of the circulation chamber is discharged to the collection end through the first collection branch pipe, the cleaning water is pumped to the corresponding nozzle through the cleaning branch pipes in the first, second and third inner cavities of the circulation chamber to rinse the adsorbent material remaining on the screen plate.

[0026] Furthermore, a first check valve is provided between the third electric valve and the spray head on the first circulation branch pipe. The first check valve is used to block the flow from the spray head to the third electric valve. A second check valve is provided between the fourth electric valve and the spray head on the second circulation branch pipe. The second check valve is used to block the flow from the spray head to the fourth electric valve.

[0027] The beneficial effects of this invention are as follows:

[0028] The protease adsorption cycle box of the present invention can simultaneously complete the cyclic adsorption of two proteases. Moreover, based on the variable multi-chamber structure and alternating circulation mode of the cycle box, the types of proteases collected can be customized to increase or decrease, and the processing volume can be controlled in a targeted manner. At the same time, during the protease adsorption process, it can ensure that the adsorbent material is always suspended in the urine, thereby improving the adsorption rate. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the interconnected structure of the protease adsorption circulation chamber of the present invention (partitions are inserted into the second slot, the fourth slot, and the sixth slot).

[0030] Figure 2 This is a schematic diagram of the interconnected structure of the protease adsorption circulation chamber of the present invention (partitions are inserted into the first slot, third slot, fourth slot, fifth slot and seventh slot);

[0031] Figure 3 This is a schematic diagram of the interconnected structure of the protease adsorption circulation chamber of the present invention (partitions are inserted into the first slot, third slot, fourth slot, and fifth slot).

[0032] Figure 4 This is a schematic diagram (top view) of the circulation tank of the present invention.

[0033] Labeling instructions: 1. Circulation tank, 1-1. First slot, 1-2. Second slot, 1-3. Third slot, 1-4. Fourth slot, 1-5. Fifth slot, 1-6. Sixth slot, 1-7. Seventh slot, 2. Inlet pipe, 3. First electric valve, 4. Drain pipe, 5. Second electric valve, 6. First pump body, 7. Second pump body, 8. First three-way valve, 9. Second three-way valve, 10. First circulation branch pipe, 11. Third electric valve, 12. First check valve, 13. Second circulation branch pipe, 14. Fourth electric valve, 15. Second check valve, 16. First collection branch pipe, 17. Fifth electric valve, 18. Second collection branch pipe, 19. Sixth electric valve, 20. Cleaning branch pipe, 21. Seventh electric valve, 22. Overflow pipe. Detailed Implementation

[0034] The invention will now be further described with reference to the accompanying drawings.

[0035] Example 1:

[0036] Please see Figure 1-4 As shown, the protease adsorption circulation box includes a circulation box 1, a first pump body 6 and a second pump body 7, a first three-way valve 8 and a second three-way valve 9.

[0037] The inner cavity of the circulation box 1 is provided with the following slots from left to right: first slot 1-1, second slot 1-2, third slot 1-3, fourth slot 1-4, fifth slot 1-5, sixth slot 1-6, and seventh slot 1-7, wherein:

[0038] Partitions are inserted into the second slot 1-2, the fourth slot 1-4 and the sixth slot 1-6 to divide the inner cavity of the circulation box 1 into the first inner cavity, the second inner cavity, the third inner cavity and the fourth inner cavity in sequence;

[0039] Alternatively, partitions can be inserted into the first slot 1-1, the third slot 1-3, the fourth slot 1-4, the fifth slot 1-5, and the seventh slot 1-7 to sequentially divide the inner cavity of the circulation box 1 into the first cavity, the first inner cavity, the second inner cavity, the third inner cavity, the fourth inner cavity, and the second cavity.

[0040] Alternatively, partitions can be inserted into the first slot 1-1, the third slot 1-3, the fourth slot 1-4, and the fifth slot 1-5 to sequentially divide the inner cavity of the circulation box 1 into the first empty cavity, the first inner cavity, the second inner cavity, the third inner cavity, and the fourth inner cavity.

[0041] In this embodiment, partitions are inserted into the second slot 1-2, the fourth slot 1-4, and the sixth slot 1-6 to divide the area inside the circulation box 1 into equal parts; partitions are inserted into the first slot 1-1, the third slot 1-3, the fourth slot 1-4, the fifth slot 1-5, and the seventh slot 1-7 to divide the area between the first slot 1-1 and the seventh slot 1-7 inside the circulation box 1 into equal parts; partitions are inserted into the first slot 1-1, the third slot 1-3, the fourth slot 1-4, and the fifth slot 1-5 to divide the area between the first slot 1-1 and the fifth slot 1-5 inside the circulation box 1 into equal parts.

[0042] The bottom of the first inner cavity of the circulation tank 1 is connected to the inlet end via an inlet pipe 2, and the inlet pipe 2 is equipped with a first electric valve 3 for controlling the opening and closing of the inlet pipe 2. The bottom of the first inner cavity of the circulation tank 1 is connected to the outlet end via a drain pipe 4, and the drain pipe 4 is equipped with a second electric valve 5 for controlling the opening and closing of the drain pipe 4. A first automatic feeding device and a second automatic feeding device are provided above the circulation tank 1, for feeding different adsorbent materials into the second and third inner cavities of the circulation tank 1, respectively. For example, when the target collected substances are urokinase and ulinastatin, the adsorbent materials are silica gel particles and chitosan, respectively. A first liquid level sensor is provided on the circulation tank 1 for monitoring the liquid level inside the circulation tank 1.

[0043] The inlet end refers to the urine storage container.

[0044] The first inner cavity of the circulation tank 1 is connected to the pump inlet of the first pump body 6 and the pump outlet of the second pump body 7. The pump outlet of the first pump body 6 and the pump inlet of the second pump body 7 are connected to the inlet end of the first three-way valve 8. The first outlet end of the first three-way valve 8 is connected to the bottom of the fourth inner cavity of the circulation tank 1. The second outlet end of the first three-way valve 8 is connected to the second outlet end of the second three-way valve 9. The inlet end of the second three-way valve 9 is connected to the bottom of the third inner cavity of the circulation tank 1. The circulation tank 1 is provided with a first circulation branch pipe 10 connected to its second inner cavity. One end of the branch pipe 10 located in the second inner cavity is equipped with a spray head. The other end of the first circulation branch pipe 10 is connected to the pump outlet of the second pump body 7, and a third electric valve 11 is provided on it to control the opening and closing of the first circulation branch pipe 10. The circulation box 1 is equipped with a second circulation branch pipe 13 connected to its third inner cavity. One end of the second circulation branch pipe 13 located in the third inner cavity is equipped with a spray head. The other end of the second circulation branch pipe 13 is connected to the first outlet end of the first three-way valve 8, and a fourth electric valve 14 is provided on it to control the opening and closing of the second circulation branch pipe 13.

[0045] Necessary is that filters are provided on both the pump inlet side of the first pump body 6 and the pump inlet side of the second pump body 7.

[0046] The bottom of the second inner cavity of the circulation box 1 is connected to the collection end through the first collection branch pipe 16, and the first collection branch pipe 16 is provided with a fifth electric valve 17 for controlling the opening and closing of the first collection branch pipe 16. The first outlet end of the second three-way valve 9 is connected to the collection end through the second collection branch pipe 18, and the second collection branch pipe 18 is provided with a sixth electric valve 19 for controlling the opening and closing of the second collection branch pipe 18.

[0047] The collection end refers to the separation container between urine and adsorbent material, while the discharge end refers to sewage facilities such as sewers and septic tanks.

[0048] In the above technical solution, the circulation tank 1 is connected to the discharge end through the overflow pipe 22 to prevent overflow. Specifically, the connection position of the overflow pipe 22 on the circulation tank 1 should be carefully considered to avoid changes in the baffle plate that would prevent it from functioning properly as an overflow pipe.

[0049] The protease adsorption circulation chamber is configured as follows, depending on the specific collection requirements:

[0050] When collecting two proteases using two adsorbent materials, the partitions inserted in the second slot 1-2, the fourth slot 1-4, and the sixth slot 1-6 in the inner cavity of the circulation chamber 1 are all sieve plates to accommodate a larger processing volume, or the partitions inserted in the first slot 1-1 and the seventh slot 1-7 are all solid plates, and the partitions inserted in the third slot 1-3, the fourth slot 1-4, and the fifth slot 1-5 are all sieve plates to accommodate a smaller processing volume. In addition, the inlet end of the first three-way valve 8 is connected to the first outlet end, and the inlet end of the second three-way valve 8 is connected to the first outlet end.

[0051] When collecting a protease using an adsorbent material, in the inner cavity of the circulation chamber 1, the partitions inserted in the second slot 1-2 and the fourth slot 1-4 are all screen plates, and the partition inserted in the sixth slot 1-5 is a solid plate to accommodate a larger processing volume. Alternatively, the partitions inserted in the first slot 1-1 and the fifth slot 1-5 are all solid plates, and the partitions inserted in the third slot 1-3 and the fourth slot 1-4 are all screen plates to accommodate a smaller processing volume. The inlet end of the first three-way valve 8 is connected to the second outlet end, and the inlet end of the second three-way valve 9 is connected to the second outlet end.

[0052] Specifically, the cyclic adsorption method of the protease adsorption cycle box, when collecting two proteases using two adsorbent materials, involves the following steps: Urine is injected into the cycle box through the inlet pipe 2; then, one adsorbent material is added to the second inner cavity of the cycle box 1 through the first automatic feeding device, and another adsorbent material is added to the third inner cavity of the cycle box 1 through the second automatic feeding device. Simultaneously, the following two steps are performed alternately according to a first preset time: urine in the first inner cavity of the cycle box 1 is injected into its third and fourth inner cavities through the first pump body 6, and urine in the fourth inner cavity of the cycle box 1 is injected into its first and second inner cavities through the second pump body 7; finally, the mixture of urine and corresponding adsorbent material in the second inner cavity of the cycle box 1 is discharged to the collection end through the first collection branch pipe 16, and the mixture of urine and corresponding adsorbent material in the third inner cavity of the cycle box 1 is discharged to the collection end through the second collection branch pipe 18.

[0053] When collecting a protease using an adsorbent material, the following steps are performed: urine is injected into the circulation tank 1 through the inlet pipe 2; then, an adsorbent material is added into the second inner cavity of the circulation tank 1 through the first automatic feeding device. At the same time, the following two steps are performed alternately according to the second preset time: urine in the first inner cavity of the circulation tank 1 is injected into its third inner cavity through the first pump body 6, and urine in the third inner cavity of the circulation tank 1 is injected into its first and second inner cavities through the second pump body 7; finally, the mixture of urine and corresponding adsorbent material in the second inner cavity of the circulation tank 1 is discharged to the collection end through the first collection branch pipe 16.

[0054] To achieve impact on the bottom adsorbent material, the spray head on the first circulation branch pipe 10 is located at the bottom of the second inner cavity of the circulation box 1, and the spray head on the second circulation branch pipe 13 is located at the bottom of the third inner cavity of the circulation box 1.

[0055] In the above-mentioned protease adsorption circulation method, urine circulates between the various cavities of the circulation box 1. Since there is a delay in the opening and closing of the third electric valve 11 and the fourth electric valve 14, in order to eliminate the influence of the delay, it is preferable that a first one-way valve 12 is provided between the third electric valve 11 and the spray head on the first circulation branch pipe 10. The first one-way valve 12 is used to block the flow from the spray head to the third electric valve 11. A second one-way valve 15 is provided between the fourth electric valve 14 and the spray head on the second circulation branch pipe 13. The second one-way valve 15 is used to block the flow from the spray head to the fourth electric valve 14.

[0056] According to the above design, during the circulation of urine between the various cavities of the circulation tank 1, the third electric valve 11 and the fourth electric valve 14 can remain open.

[0057] Furthermore, since the inlet pipe 2, the first outlet end of the first three-way valve 8, the inlet end of the second three-way valve 9, and the pump outlet of the second pump body 7 are all connected to the bottom of the corresponding inner cavity of the circulation tank 1, and the spray heads on the first circulation branch pipe 10 and the second circulation branch pipe 13 are all located at the bottom of their respective inner cavities, the number of air bubbles generated during the circulation of urine between the various inner cavities of the circulation tank 1 will be significantly reduced.

[0058] Example 2:

[0059] Please see Figure 1-4 As shown, based on Embodiment 1, the first inner cavity, the second inner cavity, the third inner cavity and the fourth inner cavity of the circulation tank 1 are all connected to the cleaning branch pipe 20. Each cleaning branch pipe 20 is equipped with a seventh electric valve 21, and each of them is equipped with a spray head at one end inside the circulation tank 1 and connected to a water source at the other end.

[0060] The water source is tap water or water tanks, etc.

[0061] When the circulation tank 1 needs initial cleaning, the cleaning water is pumped to the corresponding spray head through the corresponding cleaning branch pipe 20 and sprayed out.

[0062] To achieve complete cleaning of the container, the spray heads on the cleaning branch pipes 20 that connect the first, second, third, and fourth inner cavities of the circulation tank 1 are all located at the top of the corresponding inner cavity in the circulation tank 1.

[0063] When the circulation tank 1 needs to be cleaned as a whole, that is, the circulation tank 1 and the corresponding connecting pipeline (including the components on the pipeline) need to be cleaned, the following steps are performed;

[0064] The cleaning water in the collection end is injected into the circulation tank 1 through the liquid inlet pipe 2;

[0065] Inside the circulation tank 1, when collecting two proteases using two adsorbent materials, the following two steps are performed alternately at a third preset time: the first pump body 6 injects the cleaning water in the first inner cavity of the circulation tank 1 into its third and fourth inner cavities, and the second pump body 7 injects the cleaning water in the fourth inner cavity of the circulation tank 1 into its first and second inner cavities. Then, the cleaning water in the circulation tank 1 is injected into the collection end through the first collection branch pipe 16 and the second collection branch pipe 18 to clean the circulation tank 1 and the corresponding pipelines.

[0066] When collecting a protease using an adsorbent material, the following two steps are performed alternately at a fourth preset time: the first pump body 6 injects the cleaning water in the first inner cavity of the circulation tank 1 into its third inner cavity, and the second pump body 7 injects the cleaning water in the third inner cavity of the circulation tank 1 into its first and second inner cavities. Then, the cleaning water in the circulation tank 1 is injected into the collection end through the first collection branch pipe 16 to clean the circulation tank 1 and the corresponding pipelines.

[0067] In addition, when the circulation tank 1 requires a deep cleaning, a cleaning agent is added to the cleaning water during the cleaning process.

[0068] The cleaning agent is selected according to the cleaning requirements.

[0069] Necessary is that after the circulation tank 1 undergoes a complete deep cleaning, it needs to be cleaned at least once more. Generally, the complete deep cleaning is performed late at night.

[0070] It should be noted that the above cleaning methods should be selected according to actual production needs.

[0071] As one embodiment, the cyclic adsorption method of the protease adsorption cycle box, when collecting two proteases through two adsorbent materials, further includes the following steps: the mixture of urine and corresponding adsorbent material in the second inner cavity of the cycle box 1 is discharged to the collection end through the first collection branch pipe 16, and the mixture of urine and corresponding adsorbent material in the third inner cavity of the cycle box 1 is discharged to the collection end through the second collection branch pipe 18. Then, the cleaning water is pumped to the corresponding nozzle and sprayed out through the cleaning branch pipes 20 in the first, second, third and fourth inner cavities of the cycle box 1 to rinse the adsorbent material remaining on the screen plate. The cleaning water and the remaining adsorbent material are discharged to the collection end through the first collection branch pipe 16 and the second collection branch pipe 18.

[0072] When collecting a protease using an adsorbent material, the method further includes the following steps: after the urine mixture in the second inner cavity of the circulation tank 1 is discharged to the collection end through the first collection branch pipe 16, the cleaning water is pumped to the corresponding nozzle through the cleaning branch pipes 20 in the first, second and third inner cavities of the circulation tank 1 to rinse the adsorbent material remaining on the screen plate. The cleaning water and the remaining adsorbent material are discharged to the collection end through the first collection branch pipe 16.

[0073] In summary, the protease adsorption cycle box of the present invention can simultaneously complete the cyclic adsorption of two proteases. Moreover, based on the variable multi-chamber structure and alternating circulation mode of the cycle box 1, the types of proteases collected can be customized to increase or decrease, and the processing volume can be controlled in a targeted manner. At the same time, during the protease adsorption process, it can ensure that the adsorbent material is always suspended in the urine, thereby improving the adsorption rate.

[0074] Of course, the above are only preferred embodiments of the present invention and are not intended to limit the scope of application of the present invention. Therefore, any equivalent changes made to the principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A protease adsorption cycle box, characterized in that: Includes a circulation tank, a first pump body, a second pump body, a first three-way valve, and a second three-way valve; The inner cavity of the circulation box is provided with a first slot, a second slot, a third slot, a fourth slot, a fifth slot, a sixth slot, and a seventh slot from left to right. Partitions are inserted into the second, fourth, and sixth slots to sequentially divide the inner cavity of the circulation box into a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity; or partitions are inserted into the first, third, fourth, fifth, and seventh slots to sequentially divide the inner cavity of the circulation box into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity, and a second empty cavity; or partitions are inserted into the first, third, fourth, and fifth slots to sequentially divide the inner cavity of the circulation box into a first empty cavity, a first inner cavity, a second inner cavity, a third inner cavity, and a fourth inner cavity. The bottom of the first inner cavity of the circulation tank is connected to the inlet end through an inlet pipe, and a first electric valve is provided on the inlet pipe. The bottom of the first inner cavity of the circulation tank is connected to the outlet end through a drain pipe, and a second electric valve is provided on the drain pipe. A first automatic feeding device and a second automatic feeding device are provided above the circulation tank, which are used to feed different adsorbent materials into the second inner cavity and the third inner cavity of the circulation tank, respectively. A first liquid level sensor is provided on the circulation tank to monitor the liquid level in the circulation tank. The first inner cavity of the circulation tank is connected to the pump inlet of the first pump body and the pump outlet of the second pump body. The pump outlet of the first pump body and the pump inlet of the second pump body are connected to the inlet end of the first three-way valve. The first outlet end of the first three-way valve is connected to the bottom of the fourth inner cavity of the circulation tank. The second outlet end of the first three-way valve is connected to the second outlet end of the second three-way valve. The inlet end of the second three-way valve is connected to the bottom of the third inner cavity of the circulation tank. The circulation tank is provided with a first circulation branch pipe connected to its second inner cavity. A spray head is provided at one end of the first circulation branch pipe located in the second inner cavity. The other end is connected to the pump outlet of the second pump body and is equipped with a third electric valve. The circulation box is equipped with a second circulation branch pipe that connects to its third inner cavity. One end of the second circulation branch pipe located in the third inner cavity is equipped with a spray head. The other end of the second circulation branch pipe is connected to the first outlet end of the first three-way valve and is equipped with a fourth electric valve. The bottom of the second inner cavity of the circulation box is connected to the collection end through the first collection branch pipe and is equipped with a fifth electric valve. The first outlet end of the second three-way valve is connected to the collection end through the second collection branch pipe and is equipped with a sixth electric valve. When collecting two proteases using two adsorbent materials, the partitions inserted in the second, fourth, and sixth slots of the circulation chamber are all sieve plates, or the partitions inserted in the first and seventh slots are all solid plates, and the partitions inserted in the third, fourth, and fifth slots are all sieve plates. In addition, the inlet end of the first three-way valve is connected to the first outlet end, and the inlet end of the second three-way valve is connected to the first outlet end. When collecting a protease using an adsorbent material, the partitions inserted in the second and fourth slots of the circulation chamber are screen plates, and the partition inserted in the sixth slot is a solid plate; or the partitions inserted in the first and fifth slots are solid plates, and the partitions inserted in the third and fourth slots are screen plates. The inlet end of the first three-way valve is connected to the second outlet end, and the inlet end of the second three-way valve is connected to the second outlet end.

2. The protease adsorption circulation chamber according to claim 1, characterized in that: The first, second, third, and fourth inner cavities of the circulation tank are all connected to cleaning branch pipes. Each cleaning branch pipe is equipped with a seventh electric valve, and each has a spray head at one end inside the circulation tank and is connected to a water source at the other end.

3. The protease adsorption circulation chamber according to claim 2, characterized in that: The spray heads installed on the cleaning branch pipes connecting the first, second, third, and fourth inner cavities of the circulation box are all located at the top of the corresponding inner cavity in the circulation box.

4. The protease adsorption circulation chamber according to claim 1, characterized in that: The spray head installed on the first circulation branch pipe is located at the bottom of the second inner cavity of the circulation box, and the spray head installed on the second circulation branch pipe is located at the bottom of the third inner cavity of the circulation box.

5. The protease adsorption circulation chamber according to claim 1, characterized in that: The circulation tank is connected to the discharge end via an overflow pipe.

6. The protease adsorption cycle box according to claim 1, characterized in that: Both the pump inlet side of the first pump body and the pump inlet side of the second pump body are equipped with filters.

7. The protease adsorption circulation chamber according to claim 1, characterized in that: Partitions are inserted into the second, fourth, and sixth slots to divide the area inside the circulation box into equal parts; partitions are inserted into the first, third, fourth, fifth, and seventh slots to divide the area between the first and seventh slots inside the circulation box into equal parts; partitions are inserted into the first, third, fourth, and fifth slots to divide the area between the first and fifth slots inside the circulation box into equal parts.

8. A cyclic adsorption method for a protease adsorption cycle chamber according to any one of claims 1-7, characterized in that: When collecting two proteases using two adsorbents, the following steps are performed: Urine is injected into the circulation tank through the inlet pipe; then, one adsorbent is added to the second inner cavity of the circulation tank through the first automatic feeding device, and another adsorbent is added to the third inner cavity of the circulation tank through the second automatic feeding device. Simultaneously, the following two steps are performed alternately according to the first preset time: urine in the first inner cavity of the circulation tank is injected into its third and fourth inner cavities through the first pump, and urine in the fourth inner cavity of the circulation tank is injected into its first and second inner cavities through the second pump; finally, the mixture of urine and corresponding adsorbent in the second inner cavity of the circulation tank is discharged to the collection end through the first collection branch pipe, and the mixture of urine and corresponding adsorbent in the second inner cavity of the circulation tank is discharged to the collection end through the second collection branch pipe. When collecting a protease using an adsorbent material, the following steps are performed: urine is injected into a circulation tank through an inlet pipe; then, an adsorbent material is added to the second inner cavity of the circulation tank through a first automatic feeding device. Simultaneously, the following two steps are performed alternately at a second preset time: urine from the first inner cavity of the circulation tank is injected into its third inner cavity through a first pump, and urine from the third inner cavity of the circulation tank is injected into its first and second inner cavities through a second pump; finally, the mixture of urine and the corresponding adsorbent material in the second inner cavity of the circulation tank is discharged to the collection end through a first collection branch pipe.

9. The cyclic adsorption method of a protease adsorption cycle box according to claim 8, characterized in that: When collecting two proteases using two adsorbent materials, the process further includes the following steps: discharging the mixture of urine and corresponding adsorbent materials in the second inner cavity of the circulation chamber to the collection end through the first collection branch pipe, and discharging the mixture of urine and corresponding adsorbent materials in the third inner cavity of the circulation chamber to the collection end through the second collection branch pipe, and then pumping cleaning water to the corresponding spray head through the cleaning branch pipes in the first, second, third and fourth inner cavities of the circulation chamber to rinse the adsorbent materials remaining on the screen plate; When collecting a protease using an adsorbent material, the method further includes the following steps: after the urine mixture in the second inner cavity of the circulation chamber is discharged to the collection end through the first collection branch pipe, the cleaning water is pumped to the corresponding spray head through the cleaning branch pipes in the first, second and third inner cavities of the circulation chamber to rinse the adsorbent material remaining on the screen plate.

10. The cyclic adsorption method of a protease adsorption cycle chamber according to claim 8, characterized in that: A first check valve is provided between the third electric valve and the spray head on the first circulation branch pipe. The first check valve is used to block the flow from the spray head to the third electric valve. A second check valve is provided between the fourth electric valve and the spray head on the second circulation branch pipe. The second check valve is used to block the flow from the spray head to the fourth electric valve.

Images