Mixing systems and mixing methods

Abstract

The present invention provides a mixing system and method for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline. [Solution] The present invention relates to a mixing system (100) for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline, and comprises a pressurizing device (2: first pressurizing device), a pressure drop mechanism (4: nozzle, orifice, etc.), a cooling device (3), and a filter (5) for removing nuclei and mitochondria.

Description

【Technical Field】 【0001】 The present invention relates to a system and method for mixing stem cells and pure water. Here, the term "mixing with stem cells" includes not only mixing with stem cells alone, but also mixing with stem cells and nerve cells, mixing with stem cells and immune cells, and mixing with stem cells, nerve cells and immune cells. In addition, the term "mixing with pure water" includes mixing with either pure water or physiological saline. 【Background Art】 【0002】 Regarding stem cells, their application fields are very extensive. As a result of various studies, the inventor has found that if a physical stimulus (or shock) is applied to cells to cause mechanotransduction, which is a reaction in which the physical stimulus applied to the cells is received by the cells and converted into a biochemical signal, a variety of beneficial substances can be made available. When conducting an experiment to verify the effect of beneficial substances (stem cell-derived special molecular groups) produced in stem cells by a mechanotransduction reaction, for example, if mechanotransduction can be caused in a state where stem cells and pure water are mixed, a specimen for conducting an experiment to verify the effect of the beneficial substances can be easily obtained without performing a separate dilution step. And, for example, if mechanotransduction is caused in a state where stem cells and physiological saline are mixed, it can be expected that the beneficial effect will be sustained for a long time in a specimen for conducting an experiment to verify the effect of the beneficial substances, which is useful for an experiment to verify the effect of the beneficial substances. Furthermore, the inventor has found that if pure water and stem cells are mixed to cause mechanotransduction, an anti-aging agent that improves the hormone regulation ability can be produced. An anti-aging agent that has the effect of improving the hormone regulation ability can enable the self-renewal of stem cells as in youth, and it can be expected that the self-renewal of stem cells can be made possible as in youth even when the self-renewal of stem cells becomes difficult with aging. However, no technology has been proposed at present for a mixture of stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells, and immune cells mixed with pure water or physiological saline to induce mechanotransduction. 【0003】 Various drugs that have been processed using stem cells have been proposed, and for example, there are oocyte maturation promoters that use the filtrate obtained by disrupting stem cells as the active ingredient (see, for example, Patent Document 1). However, the conventional technology described herein is an egg maturation promoter, not an anti-aging agent that improves hormone regulation ability. Furthermore, it does not disclose anything about mixing mechanotransduction-induced stem cells with pure water, physiological saline, or cell culture medium. [Prior art documents] [Patent Documents] 【0004】 [Patent Document 1] Patent No. 7257012 [Overview of the Initiative] [Problems that the invention aims to solve] 【0005】 This invention was proposed in view of the problems of the prior art described above, and aims to provide a mixing system and mixing method for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline. [Means for solving the problem] 【0006】 The mixing system (100) of the present invention is In a mixing system (100) that mixes stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline, A raw material source (10) that supplies raw materials consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells and immune cells, A liquid supply source (11) that supplies pure water at a rate of 0.7 mL to 1.3 mL per 1 million stem cells, or physiological saline at a rate of 0.8 mL to 1.5 mL per 1 million stem cells, To induce mechanotransduction in stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells, and immune cells, a raw material (stem cells, etc.) consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells, and a liquid that is pure water or physiological saline. 25-200 MPa A pressurizing device (2: First pressurizing device), A pressure loss mechanism (4: nozzle, orifice, etc.) through which the pressurized raw material and liquid pass and which causes pressure loss as they pass, The system includes a cooling device (3) for cooling the raw material and the liquid and / or the pressure drop mechanism (4) that are pressurized by the pressurizing device (2), The nucleus and mitochondria (of cells) are removed from the raw material that has passed through the pressure drop mechanism (4). Therefore, it is possible to perform filtration with a coarseness of 0.2 μm to 0.3 μm. Filter (5) and, The device includes a temporary storage device (12) located on the pressure loss mechanism (4) side of the filter (5) for reducing the pressure of the raw material that has passed through the pressure loss mechanism (4), To generate mechanotransduction in the raw material by pressurizing it multiple times and causing pressure loss over multiple cycles, a three-way valve (V30) is provided in the region between the pressure loss mechanism (4) and the temporary storage device (12), a path (R20) is provided connecting the three-way valve (V30) and the inlet side of the pressurizing device (2), and a control unit (50) is provided to determine whether the number of pressurization and pressure loss cycles for the raw material is a predetermined number. It is characterized by possessing something. Here, mechanotransduction is a reaction in which a physical stimulus (or impact) is applied to a cell, the physical stimulus applied to the cell is received by the cell, and converted into a biochemical signal. In this invention, mechanotransduction does not include heating, even if it involves a physical stimulus or impact. Furthermore, while adipose-derived stem cells can be used as stem cells, other types of stem cells (such as umbilical cord blood-derived stem cells) can also be used. 【0007】 In the mixing system (100-3) of the present invention, The system has a mineral water source that supplies mineral water containing one of magnesium, potassium, calcium, or sodium, and 10 mL of the mineral water contains one of the following: magnesium 2.7-3.0 mg, potassium 1.2-1.5 mg, calcium 1.1-1.4 mg, or sodium 27-29 mg. The mixture of the raw material and the liquid that has passed through the filter (5) Add 10 mL of the mineral water to 1 mL to 2 mL of the above-mentioned mineral water. It is preferable to have a mixing device (8) for mixing. Furthermore, in the mixing system (100-1) of the present invention, it is preferable to have a nasal spray dispensing device (6) for dispensing the raw materials that have passed through the filter (5) into a nasal spray. In addition, in the mixing system (100-2) of the present invention, it is preferable to have an eye drop container dispensing device (7) for dispensing the raw material that has passed through the filter (5) into an eye drop container. 【0008】 In addition, the mixing method of the present invention is In a method of mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells and nerve cells and immune cells with pure water or physiological saline, A step of supplying raw materials from a raw material source (10) consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells and immune cells, A step of supplying pure water from a liquid supply source (11) at a rate of 0.7 mL to 1.3 mL per 1 million stem cells, or supplying physiological saline at a rate of 0.8 mL to 1.5 mL per 1 million stem cells, In order to cause mechanotransduction in stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells and nerve cells and immune cells, a raw material composed of any of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells and nerve cells and immune cells and a liquid that is pure water or physiological saline are pressurized by a pressurizing device (first pressurizing device 2) 25-200 MPa The step of pressurizing, including a pressure loss step of causing a pressure loss in the raw material and the liquid pressurized by the pressurizing device (2) by passing through a pressure loss mechanism (4: nozzle, orifice, etc.), In the pressurizing step and / or the pressure loss step, a cooling step of cooling the pressurized raw material, the liquid, and / or the pressure loss mechanism (4) is performed, A filter (5) with a coarseness capable of filtering through 0.2 μm to 0.3 μm, A filtering step of removing nuclei and mitochondria from the raw material in which a pressure loss has occurred in the pressure loss step and, On the pressure drop mechanism side of the filter (5), there is a step of reducing the pressure of the raw material that has passed through the pressure drop mechanism (4) using a temporary storage device (12), A process to generate mechanotransduction in the raw material multiple times, comprising a three-way valve (V30) provided in the region between the pressure drop mechanism (4) and the temporary storage device (12), a path (R20) connecting the three-way valve (V30) and the inlet side of the pressurizing device (2), and a control unit (50) that determines whether the number of times pressurization and pressure drop on the raw material is a predetermined number. It is characterized by having. In the production method of the present invention, it is preferable to have a nasal spray dispensing step of dispensing the raw material that has undergone the filtering step into a nasal spray. In addition, in the production method of the present invention, it is preferable to have an eye drop container dispensing step of dispensing the raw material that has undergone the filtering step into an eye drop container. And in the production method of the present invention, the mixture of the raw material and the liquid that has passed through the filter (5) The process involves mixing 10 mL of mineral water with 1 mL to 2 mL of mineral water using a mixing device (8), and the minerals contained in the mineral water are Magnesium, potassium, calcium, sodium In the case of 10 mL of the mineral water, the magnesium content is 2.7-3.0 mg, the potassium content is 1.2-1.5 mg, the calcium content is 1.1-1.4 mg, or the sodium content is 27-29 mg.is preferred. 【Advantages of the Invention】 【0009】 In the present invention having the above-described configuration, physical stimulation is applied to stem cells or the like (any one of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells: raw material), and mechano-transduction, which is a reaction in which the physical stimulation applied to the cells is received by the cells and converted into a biochemical signal, is generated. Nuclei and mitochondria are removed from the stem cells or the like (raw material) in which mechano-transduction has occurred. Then, components or beneficial substances that assist tissue regeneration, such as growth factors (cytokines), exosomes, proteins, etc., can be utilized from the stem cells or the like in which mechano-transduction has occurred. Since the stem cells or the like (raw material) and a liquid (pure water or physiological saline) are mixed, a specimen for conducting an experiment to verify the effect of the beneficial substance can be easily obtained without separately diluting it. And, for example, if mechano-transduction is generated in a state where stem cells or the like and physiological saline are mixed, in a specimen for conducting an experiment to verify the effect of the beneficial substance, it can be expected that the beneficial effect will be maintained for a long period, which is useful for the experiment to verify the effect of the beneficial substance. In addition, when mixed by the mixing system and the mixing method of the present invention, a mixture that improves the properties required as an anti-aging agent, that is, the hormone-regulating ability, can be obtained. 【0010】 In the present invention, when applying stimulation to stem cells or the like, by passing through a pressure loss mechanism (4: nozzle, orifice, etc.) in a state of being pressurized to a high pressure to cause pressure loss, physical stimulation necessary for the stem cells or the like is applied to generate mechano-transduction. Here, applying high pressure to stem cells, etc., may cause the stem cells, etc., to heat up and undergo thermal denaturation. Similarly, causing pressure loss in the pressurized raw material may cause the stem cells, etc., to heat up and undergo thermal denaturation. In contrast, in the present invention, the pressurized raw material and / or the pressure loss mechanism (4) are cooled by the cooling device (3), thereby preventing the stem cells, etc., from undergoing thermal denaturation due to heating caused by pressurization or pressure loss. 【0011】 In this invention, stem cells and the like that, which have passed through the pressure drop mechanism (4) and undergone mechanotransduction upon receiving physical stimulation (physical stimulation other than heating, such as impact), have their nuclei and mitochondria removed by the filter (5). Therefore, unnecessary nuclei and mitochondria are reliably removed in experiments verifying the effects of the beneficial substances. Furthermore, nuclei and mitochondria are also unnecessary for anti-aging agents. 【0012】 In this invention, if mineral water containing magnesium, potassium, calcium, and sodium is mixed with stem cells, etc., from which the nucleus and mitochondria have been removed by passing through the filter (5), the mineral concentration of the mixture can be made to be similar to the mineral concentration in the extracellular fluid. For example, when the mixture is used as an anti-aging agent, it is expected that the permeability of the anti-aging agent into cells will be increased, allowing the agent to penetrate into the cells of the user to whom it is administered, thereby promoting the regeneration of stem cells. Furthermore, when using a mixture as a test specimen in an experiment, mixing it with mineral water can be expected to prolong the beneficial effects of the beneficial substance in the test specimen used for experiments verifying the effects of the beneficial substance. [Brief explanation of the drawing] 【0013】 [Figure 1] This is a block diagram of a mixing system according to the first embodiment of the present invention. [Figure 2] This is a flowchart showing the procedure for the mixing method using the mixing system shown in Figure 1. [Figure 3]This is a block diagram of a mixing system according to a second embodiment of the present invention. [Figure 4] This is a flowchart showing the procedure for the mixing method using the mixing system shown in Figure 3. [Figure 5] This is a block diagram of a mixing system according to a third embodiment of the present invention. [Figure 6] This is a flowchart showing the procedure for the mixing method using the mixing system shown in Figure 5. [Figure 7] This is a block diagram of a mixing system according to a fourth embodiment of the present invention. [Figure 8] This is a flowchart showing the procedure for the mixing method using the mixing system shown in Figure 7. [Modes for carrying out the invention] 【0014】 Embodiments of the present invention will be described below with reference to the attached drawings. First, a first embodiment of the present invention will be described with reference to Figures 1 and 2. In Figure 1, the mixed system according to the first embodiment is shown as a whole by reference numeral 100. In the mixed system 100 of the first embodiment shown in Figure 1, there are four types of stem cells, etc. That is, Stem cells only, A mixture of stem cells and nerve cells, A mixture of stem cells and immune cells, A mixture of stem cells, nerve cells, and immune cells. These four types are supplied as raw materials (stem cells, etc.) from the raw material supply source 10 to the raw material injection mechanism 9 via route R1. In other words, the mixture mixed in mixing system 100 consists of substances within the cell membrane of stem cells where mechanotransduction has occurred, but which do not include the nucleus and mitochondria. Substances within the cell membrane of stem cells and nerve cells that have undergone mechanotransduction, excluding the nucleus and mitochondria. Substances within the cell membrane of stem cells and immune cells that have undergone mechanotransduction, but which do not include the nucleus and mitochondria, or It includes any of the following: substances within the cell membrane of stem cells, nerve cells, and immune cells in which mechanotransduction has occurred, which do not include the nucleus and mitochondria. In the illustrated embodiment, stem cells derived from adipose tissue are used, but stem cells are not limited to those derived from adipose tissue; stem cells derived from umbilical cord blood or other sources can also be used. 【0015】 In Figure 1, the mixing system 100 according to the first embodiment includes a raw material supply source 10, a liquid supply source 11, a raw material injection mechanism 9, a pressurizing device 2 (first pressurizing device), a cooling device 3, a pressure drop mechanism 4, a temporary storage device 12, a filter 5, and a nasal spray dispensing device 6. The raw material supply source 10 can supply any of the four types of raw materials mentioned above (stem cells only, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells) to the raw material injection mechanism 9. In other words, in the mixing system 100 of the first embodiment shown in Figure 1, any of the four types of raw materials can be mixed with a liquid. In this specification, the four types of raw materials described above may be referred to as "raw materials for stem cells, etc." or "stem cells, etc." 【0016】 The liquid supply source 11 is either pure water or physiological saline. When the mixing system 100 according to the illustrated embodiment is used for the manufacture of an anti-aging agent, the liquid supply source 11 supplies, for example, pure water. Since pure water does not contain other substances, it is convenient not only when used for the manufacture of anti-aging agents but also when used for the manufacture of test specimens. Furthermore, when used for preparing test specimens for experiments, the liquid supply source 11 supplies physiological saline. The choice of supplying pure water or physiological saline is not limited to the above, but will be determined on a case-by-case basis depending on the details of the anti-aging agent, the purpose and type of the experiment, etc. 【0017】 In addition to being supplied with raw materials such as stem cells from the raw material supply source 10 mentioned above, the raw material injection mechanism 9 is also supplied with pure water or physiological saline via pathway R2 from the liquid supply source 11. This improves the fluidity of the raw materials (stem cells, etc.). When supplying pure water using the raw material injection mechanism 9, 0.7 mL to 1.3 mL, preferably 1 mL, of pure water is added for every 1 million stem cells, etc. According to the inventor's experiments, if the amount of pure water added is less than 0.7 mL per 1 million stem cells, the fluidity of the mixture of stem cells and water decreases. On the other hand, if the amount of pure water added is more than 1.3 mL per 1 million stem cells, the amount of stem cells becomes relatively small, and when the mixture is used as an anti-aging agent, it may not exhibit the effect of improving hormone regulation ability. 【0018】 0.8 mL to 1.5 mL, preferably 1.2 mL, of physiological saline solution is added per 1 million stem cells. According to the inventor's experiments, if the amount of physiological saline is less than 0.8 mL per 1 million stem cells, the fluidity of the mixture becomes poor, making it unsuitable as a test specimen. On the other hand, if the amount of physiological saline exceeds 1.5 mL per 1 million stem cells, the beneficial effect of the aforementioned substance may not be exerted in the stem cells that undergo mechanotransduction. 【0019】 The raw materials, such as stem cells, injected into the raw material injection mechanism 9, along with pure water or physiological saline, are supplied to the pressurizer 2 via pathway R3. Alternatively, pure water or physiological saline can be supplied from the liquid supply source 11 to pathway R3 via pathway R2-1, as shown by the dotted line in Figure 1. In this specification and in the drawings, pure water or physiological saline may be collectively referred to as "liquid." 【0020】 In pressurization device 2 (first pressurization device), raw materials such as stem cells are pressurized to 25-200 MPa, for example, up to 100 MPa. For example, a plunger pump can be used as the pressurizing device 2. However, it is not limited to a plunger pump. The pressurization by the pressurizing device 2 is set to a pressure such that mechanotransduction occurs in the stem cells or other raw materials as they pass through the pressure loss mechanism 4 described later. According to the inventor's experiments, if the pressurization by pressurizer 2 is less than 25 MPa, sufficient physical impact cannot be applied to the raw materials such as stem cells, and mechanotransduction does not occur. On the other hand, it was confirmed that if the pressurization is higher than 200 MPa, the stem cells may undergo thermal denaturation. 【0021】 When the raw materials such as stem cells are pressurized (25-200 MPa) by the pressurizing device 2, the temperature of the stem cells may rise, potentially causing thermal denaturation and coagulation. To prevent such thermal denaturation and coagulation, in the illustrated embodiment, the materials are cooled by the cooling device 3. Cooling by the cooling device 3 prevents the temperature of the raw materials such as stem cells, which have been pressurized by the pressurizing device 2, from rising and undergoing thermal denaturation. Here, in the pressure loss mechanism 4 where the stem cells and other raw materials are crushed and emulsified, the stem cells and other materials that have been subjected to pressure loss are also heated. Therefore, in the illustrated embodiment, the cooling device 3 is configured to cool the entire region from the pressurizing device 2 to the pressure loss mechanism 4 where the stem cells and other raw materials are crushed and emulsified. On the other hand, in the region downstream from the pressure loss mechanism 4 (away from the raw material injection mechanism 9), the stem cells and other materials (raw materials) are not pressurized and are not heated (overheated), so cooling by the cooling device is not performed. In Figure 1, the refrigerant supply port in the cooling device 3 is indicated by reference numeral 3A, and the refrigerant discharge port is indicated by reference numeral 3B. With respect to the cooling device 3, there are no particular limitations on its specifications, type, etc., as long as it can cool the heated raw materials and / or pressure drop mechanism 4 to a temperature at which the stem cells do not undergo thermal denaturation. 【0022】 In Figure 1, pressurized raw materials such as stem cells are supplied to a pressure loss mechanism 4 via a cooling device 3. A nozzle, orifice, etc., are used as the pressure loss mechanism 4. The pressure loss mechanism 4 is a mechanism that generates pressure loss when a fluid (flowing body) passes through it, and this pressure loss acts on the raw materials such as stem cells as they pass through the pressure loss mechanism 4. Due to the pressurization by the pressurizing device 2 and the pressure loss when passing through the pressure loss mechanism 4, physical force acts on the raw materials such as stem cells, causing them to be pulverized to molecular aggregate units (molecular group units). The physical impact applied during this pulverization induces mechanotransduction in the stem cells, causing biochemical signals to act on the inside of the stem cell membrane, resulting in alteration. It is presumed that this alteration of stem cells through mechanotransduction leads to the inclusion of abundant components such as cytokines, exosomes, and proteins that aid in tissue regeneration inside the cell membrane of the stem cells. In this manner, the cell membrane of stem cells is destroyed by the pressurization by the pressurizing device 2 and the pressure loss when passing through the pressure loss mechanism 4. In other words, the pressure loss mechanism 4 is set up to impose a pressure loss on the stem cells, etc., that is equivalent to the pressure loss that occurs in the stem cells, etc., during mechanotransduction. 【0023】 In the illustrated embodiment, in order to induce mechanotransduction in stem cells, etc., and to act on the inside of the stem cell membrane with biochemical signals to destroy the cell membrane and reliably alter the inside of the cell membrane, it is preferable to pressurize multiple times to cause pressure loss over multiple cycles. In the first embodiment shown in Figure 1, a three-way valve V30 is interposed in the path R5 through which the mixture of stem cells, etc. that have experienced pressure loss in the pressure loss mechanism 4 and pure water or physiological saline flows towards the temporary storage device 12. To cause pressure loss by pressurizing several times, the stem cells, etc. that have left the pressure loss mechanism 4 flow from the three-way valve V30 through path R20 to path R3 and are pressurized again by the pressurizing device 2. The three-way valve V30 is controlled by the control unit 50, which has the function of determining whether the number of pressurization and pressure loss cycles has reached a predetermined number of times, and if the predetermined number of times has not been reached, it switches the three-way valve V30 to communicate with the path R20 side, and if the number of cycles has exceeded the predetermined number, it switches the three-way valve V30 to the temporary storage device 12 side. 【0024】 Although not explicitly shown in the diagram, whether the number of pressurization and pressure loss cycles is a predetermined number can be determined, for example, by installing a sensor (not shown) in the path R20 and measuring the pressure and its fluctuations within the path R20. Alternatively, it can be determined by measuring the time and number of times that stem cells, etc., pass through the path R20. Or, it is possible to determine this from the time and number of times that stem cells, etc., are pressurized by a sensor (not shown) installed in the pressurization device 2, or from the time and number of times that pressure loss occurs in the mixture of stem cells, etc., and liquid (pure water or physiological saline) by a sensor (not shown) installed in the pressure loss mechanism 4. Furthermore, if the raw materials such as stem cells are intermittently supplied from the raw material supply source 10 to the material injection mechanism 9, and mixing is performed in a batch manner every cycle, the number of pressurization and pressure loss cycles can be determined by the elapsed time since the raw materials such as stem cells were supplied to the material injection mechanism 9. In other words, the method of determining or deciding the number of pressurization and pressure loss cycles is not particularly limited. Furthermore, the number of repetitions of pressurization and depressurization (a predetermined number N) varies on a case-by-case basis depending on parameters such as the type and quantity of stem cells. For example, the number of repetitions of pressurization and depressurization can be set to 3 to 4 times. However, if the number of repetitions is too low, mechanotransduction may not occur in the stem cells, biochemical signals may not act on the inside of the stem cell membrane, and the inside of the cell membrane may not be altered. On the other hand, if the number of repetitions is too high, the stem cells may degenerate, and components such as cytokines, exosomes, and proteins produced by mechanotransduction may lose their properties that help tissue regeneration. 【0025】 As described above, once the number of pressurization and pressure loss cycles reaches a predetermined number, the three-way valve V30 is switched to the temporary storage device 12 side, and the stem cells, etc. that have been pressurized and pressure lost are transferred to the temporary storage device 12. Even though the pressure loss mechanism 4 reduces the pressure of the stem cells and other raw materials, they still maintain high pressure. If such high-pressure raw materials pass through the subsequent filter 5, there is a risk of damaging the filter 5. Therefore, in the illustrated embodiment, the high-pressure raw material solution is depressurized by storing the raw materials such as stem cells in the temporary storage device 12 after pressurization. In other words, the temporary storage device 12 has the function of depressurizing the high-pressure raw material solution to prevent damage to the filter 5. Specifically, the temporary storage device 12 is provided with a depressurization mechanism 13, and the depressurization mechanism 13 is equipped with a depressurization valve 13A. Pressurized stem cells, etc. (raw material, raw material solution) are pressurized and depressurized, crushed, emulsified, and mechanotransduction occurs. After this, they are continuously or intermittently introduced (supplied) into the temporary storage device 12, where the pressure is reduced. The crushed, emulsified, and reduced-pressure stem cells, etc. (raw material, raw material solution from which mechanotransduction has occurred) are then supplied from the temporary storage device to the filter 5 via path R6 (discharge path). In the illustrated embodiment, the upstream side of the temporary storage device 12 (the side of the raw material injection mechanism 9) is not provided with a depressurization mechanism to reduce the pressure of the raw material in order to prevent damage to the filter 5. 【0026】 In Figure 1, a check valve 14 and a filter supply pressurizing device 15 (second pressurizing device) are interposed in the path R6 (discharge path) from the temporary storage device 12 to the filter 5. The check valve 14 is provided to prevent the raw materials such as stem cells from flowing back into the depressurized temporary storage device 12, and the filter supply pressurizing device 15 (second pressurizing device) is provided to press (push out) the raw materials such as stem cells toward the filter 5 so that filtration by the filter 5 is carried out sufficiently. Filter 5 is set to a coarseness necessary to remove the nucleus and mitochondria from raw materials such as stem cells that have undergone mechanotransduction, and more specifically, it is set to a coarseness that can perform filtration of 0.2 μm to 0.3 μm. According to the inventor's experiments, if the filter was coarser than 0.3 μm, it was difficult to adequately remove the nucleus and mitochondria. On the other hand, with filters finer than 0.2 μm, clogging occurred when filtering stem cells and other materials, making efficient filtration difficult. As described above, by passing through filter 5, the nucleus and mitochondria of stem cells and other cells are removed. The raw material of stem cells and other cells that has passed through filter 5 contains intracellular substances of stem cells and other cells, but the nucleus and mitochondria have been removed. 【0027】 Stem cells and other cells that pass through filter 5 undergo mechanotransduction, and furthermore, unnecessary nuclei and mitochondria (for use as anti-aging agents or experimental subjects) are removed, thus meeting the requirements for use as anti-aging agents or experimental subjects. In Figure 1, the stem cells that have passed through filter 5 are stored in storage facility 110, where they undergo further processing. The storage facility 110 only needs to have a structure that does not impair the function of growth factors (cytokines), exosomes, proteins, and other components or beneficial substances that aid in tissue regeneration in the stem cells that have undergone mechanotransduction. 【0028】 Experiments conducted by the inventors confirmed that users who used the mixture that had passed through filter 5 (a mixture from which the nucleus and mitochondria had been removed) showed an improvement in the ratio of triiodothyronine (T3: energy hormone) to reverse triiodothyronine (rT3), or "T3 / rT3," and thus improved hormone regulation ability. The inventor's experiments used thyroid hormones, more specifically the ratio of triiodothyronine (T3) to reverse triiodothyronine (rT3), or "T3 / rT3," as an indicator of whether or not stem cells were capable of self-renewal. Thyroid hormone levels are regulated by a negative feedback mechanism to remain within an appropriate range. Normally, thyroid hormone secretion is controlled by thyroid-stimulating hormone (TSH), but stress can cause abnormal TSH levels, damaging mitochondria. As a result, energy production decreases, metabolic activity declines, and cellular activity decreases. When you experience stress, the stress hormone cortisol is secreted in excess, which interferes with the production of thyroid-stimulating hormone and inhibits the production of another thyroid hormone called thyroxine (T4). In order for thyroxine (T4) to be utilized in the body, it must be converted into its active hormone, triiodothyronine (T3: an energy hormone). If thyroxine (T4) cannot be effectively converted to triiodothyronine (T3), or if there is not enough thyroxine (T4), it is converted to the inactive hormone reverse triiodothyronine (rT3). Triiodothyronine (T3) promotes metabolism, while reverse triiodothyronine (rT3) inhibits metabolism. 【0029】 If the balance between triiodothyronine (T3) and reverse triiodothyronine (rT3), or "T3 / rT3," is not appropriate, sufficient energy production is inhibited, negatively affecting bodily functions. Furthermore, if reverse triiodothyronine (rT3) levels are high and triiodothyronine (T3: energy hormone) levels are insufficient, progesterone levels will be low, estrogen will become dominant, and estrogen levels in the body will far exceed progesterone levels, leading to a metabolic state that accelerates aging. Therefore, the ratio of triiodothyronine (T3) to reverse triiodothyronine (rT3) in thyroid hormones, known as "T3 / rT3," is a very important indicator for evaluating the body's metabolic state. The following values ​​are often used as ideal guidelines for "T3 / rT3". Ideal value: 10-15 Calculation method: T3(ng / dL) / rT3(ng / dL) 【0030】 Forty subjects were included in the study, and 20 of them received a mixture (stem cell-derived mixture) prepared using the mixing system 100 shown in Figure 1 as a nasal spray twice a day, morning and evening, for three months, by spraying it into both nostrils. The aforementioned stem cell-derived mixture was mixed using the mixing system 100 shown in Figure 1, at a ratio of 1 mL of pure water to 1 million adipose-derived stem cells. The ratio of triiodothyronine (T3) to reverse triiodothyronine (rT3), or "T3 / rT3," among 40 subjects was 9.0 points on average before the mixture was administered to 20 subjects. After administering the mixture to 20 subjects over a period of three months, the ratio "T3 / rT3" in the 20 subjects who received the mixture as a nasal spray improved from an average of 9.0 points to 13.2 points. On the other hand, no such improvement was observed in the other 20 subjects who did not receive the mixture as a nasal spray, and the ratio "T3 / rT3" remained at an average of 9.0 points. From this, it was revealed that when a mixture prepared using the mixing system 100 in Figure 1, with a ratio of 1 million adipose-derived stem cells to 1 mL of pure water, is administered, the ratio "T3 / rT3" improves, thus improving the hormone regulation ability of the recipient. Furthermore, the above experiment clearly shows that the mixture prepared using the mixing system 100 in Figure 1 brings about an improvement in hormone regulation ability, and since it exhibits the effects required for an anti-aging agent, it can be expected to be an anti-aging agent. 【0031】 In an experiment separate from the one described above, the inventors administered a mixture of adipose-derived stem cells and pure water, mixed using the mixing system 100 shown in Figure 1, at a ratio of 1 mL of pure water to 1 million stem cells, along with the culture supernatant of the stem cells, as a nasal spray to two different groups of subjects (two groups of 20 subjects each) twice a day, morning and evening, for three months. Here, the contents of the culture supernatant of the aforementioned stem cells may contain trace amounts of stem cell-derived factors, or none at all. These are extremely small amounts of over 200 types of artificial additives. The types and amounts of these artificial additives vary greatly depending on the manufacturer of the culture medium. In another experiment conducted by the inventor, administering culture supernatants of multiple types of stem cells cultured in multiple types of culture media to subjects did not reveal any significant differences depending on the type of culture medium. 【0032】 As a result, the ratio of triiodothyronine (T3) to reverse triiodothyronine (rT3), or "T3 / rT3," in subjects in the group administered cellular supernatant improved on average from 9.2 points (before administration) to 12.0 points (3 months after administration). In contrast, the T3 / rT3 ratio in subjects who received a mixture of stem cells mixed with pure water using the mixing system 100 improved on average from 8.9 points (before administration) to 12.8 points (3 months after administration). In other words, the mixture of stem cells and pure water mixed by the mixing system 100 resulted in a greater improvement in T3 / rT3 than when the stem cell culture supernatant was administered. As a result, it was found that the mixture of stem cells and pure water mixed by the mixing system 100 improved T3 / rT3 and hormone regulation ability more effectively than when administering the supernatant of stem cell culture media, thus better achieving the properties required for an anti-aging agent. 【0033】 The above experiment was conducted with a mixture of adipose-derived stem cells and pure water, but in another experiment by the inventor, a mixture of stem cells other than adipose-derived stem cells and physiological saline solution also showed improved hormone regulation ability, similar to the mixture of adipose-derived stem cells and pure water. In other experiments by the inventors, when a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells was administered after being mixed with pure water using the mixing system 100 shown in Figure 1, the hormone regulation ability of the subjects who received the mixture was improved. Similarly, when each of the following mixtures—a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells—was mixed with physiological saline using the mixing system 100 shown in Figure 1, the hormone-regulating capacity was also improved. 【0034】 The procedure for mixing using the mixing system 100, as described with reference to Figure 1, will be explained primarily with reference to Figure 2. In Figure 2, in step S1, stem cells, etc. (any of the following: stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells: raw materials) are supplied from the raw material supply source 10 to the raw material injection mechanism 9. Also, pure water or physiological saline (liquid) is supplied from the supply source 11 to the raw material injection mechanism 9 or pathway R3. Then the process proceeds to step S2. In this case, when the mixing system 100 in Figure 1 is used for the manufacture of anti-aging agents, the liquid supply source 11 is supplied with, for example, pure water. When the mixing system 100 in Figure 1 is used for the manufacture of test specimens, the liquid supply source 11 is supplied with, for example, physiological saline solution. However, the choice of whether to supply pure water or physiological saline solution is not limited to the above, and will vary on a case-by-case basis depending on the details of the anti-aging agent, the purpose and type of the experiment, etc. 【0035】 In step S2 of Figure 2, the number of times N (the number of times steps S3 to S5 are executed) that the stem cells, etc., have been subjected to pressure or pressure damage at that point is set to "N=1". Then proceed to step S3. In step S3, the raw materials such as stem cells are pressurized by the pressurizing device 2 (e.g., a plunger pump) (pressurization process by pressurizing device 2). During pressurization, the pressure is set to a level that causes mechanotransduction in the raw materials such as stem cells by passing through the pressure loss mechanism 4 (step S5 described later), specifically in the range of 25 MPa to 200 MPa (e.g., 100 MPa). 【0036】 In step S4, the mixture of raw materials such as stem cells, which were pressurized in step S3 (by the pressurizing device 2), and a liquid (either pure water or physiological saline) is cooled by the cooling device 3 (cooling step). Then the process proceeds to step S5. As described above, the cooling by the cooling device 3 is performed to suppress the temperature rise of the pressurized raw materials such as stem cells and to prevent thermal denaturation of the stem cells, and cooling takes place in the path between the pressurizing device 2 and the pressure loss mechanism 4 and / or in the pressure loss mechanism 4. In particular, since there is a risk of high heat generation when pressure loss occurs in the pressure loss mechanism 4, it is preferable to cool the pressure loss mechanism 4. In step S5, the raw materials such as stem cells (raw materials mixed with liquid), which were pressurized in step S3 (by the pressurizing device 2) and cooled in step S4 (by the cooling device 3), are passed through the pressure loss mechanism 4 (nozzle, orifice, etc.) to cause pressure loss (pressure loss process). The stem cells that have passed through the pressure loss mechanism 4 are pulverized to molecular aggregate units, undergoing mechanotransduction and altering their properties. 【0037】 In the next step, S6, it is determined whether the number of times N (number of repetitions) the processes S3 to S5 have been performed on the raw material that was pressurized in step S3, cooled in step S4, and crushed in step S5 is equal to or greater than a predetermined number. This determination can be made, for example, by the control unit 50, or by other control equipment not shown. Of course, the determination can also be made by an operator. If the number of repetitions N is greater than or equal to a predetermined value (step S6 is Yes), proceed to step S8. If the number of repetitions N is less than a predetermined value (step S6 is No), proceed to step S7. In step S7, increase the number of repetitions N by 1 and proceed to step S3, repeating steps S3 to S5 (a loop where step S6 is No). In step S8, the raw materials such as stem cells, which have been pulverized and altered by mechanotransduction (by the pressure loss mechanism 4), are introduced (supplied) into the temporary storage device 12. The raw materials such as stem cells introduced into the temporary storage device 12 are depressurized by the depressurization mechanism 13 to prevent damage to the filter 5. 【0038】 In step S9, the raw materials such as stem cells, which were pulverized by the pressure drop mechanism 4 in step S5, undergoing mechanotransduction and alteration, and then stored in the temporary storage device 12 in step S8 and subjected to reduced pressure, are supplied from the temporary storage device 12 to the filter 5. Then, in step S10, the filter 5 removes the nucleus and mitochondria (cells) from the raw materials such as stem cells supplied from the temporary storage device 12 (filtration step). In the next step, S11, the raw material filtered in step S10 (raw material filtered by filter 5 to remove the nucleus and mitochondria) is stored in the storage facility 110. Although not shown in the diagram, necessary follow-up processing is performed on the filtered raw material in the storage facility 110. Here, the follow-up processing differs depending on whether the mixture from the mixing system 100 in Figure 1 is used for the manufacture of an anti-aging agent or for the manufacture of test specimens. Furthermore, it varies on a case-by-case basis depending on the specifications of the anti-aging agent or test specimen. 【0039】 A second embodiment of the present invention will be described with reference to Figures 3 and 4. When using a mixture from the mixing system according to the illustrated embodiment as an anti-aging agent, in the mixing system 100-1 of Figure 3, the mixture of stem cells and liquid is supplied to the nasal spray dispenser 6. The second embodiment shown in Figures 3 and 4 is the same as the first embodiment shown in Figures 1 and 2, except for the nasal spray dispensing device 6. In the third embodiment shown in Figures 3 and 4, redundant explanations of parts similar to the first embodiment are omitted. Furthermore, in Figure 3, which shows a block diagram of the mixing system 100-1 of the second embodiment, components similar to those shown in the mixing system 100 of the first embodiment are denoted by the same reference numerals. 【0040】 In Figure 3, the mixture of stem cells (raw material) and liquid that has passed through filter 5 is dispensed in appropriate amounts into nasal spray bottles (not shown) by nasal spray dispenser 6. Dispensing can be performed automatically using a dedicated device (not shown) (for example, a commercially available product: product name "Autopipette (product name) (manufactured by ICOMS LABO Co., Ltd.)"). When anti-aging agents are administered via nasal spray, they can easily penetrate the brain through the nasal mucosa. Therefore, they are effective in treating Alzheimer's disease and stroke. In Figure 4, which shows the process in the embodiment of Figure 3, steps S1 to S10 are the same as in Figure 2. In Figure 4, in step S12 following step S10, the raw material filtered in step S10 (raw material filtered by filter 5 to remove the nucleus and mitochondria) is dispensed as an anti-aging agent into a nasal spray by a nasal spray dispenser 6 (nasal spray dispensing step). The other configurations and effects of the second embodiment shown in Figures 3 and 4 are the same as those of the first embodiment shown in Figures 1 and 2. 【0041】 A third embodiment of the present invention will be described with reference to Figures 5 and 6. In the third embodiment, when the mixture from the mixing system according to the illustrated embodiment is used as an anti-aging agent, as shown in Figure 5, the mixture of the raw material (raw material from which mechanotransduction has occurred) from which the nucleus and mitochondria of stem cells etc. have been removed by the filter 5 and the liquid is supplied to the eye drop container dispensing device 7 via the pathway R7. The eye drop container dispensing device 7 then fills (dispenses) appropriate amounts into eye drop containers (not shown). The eye drop container dispensing device 7 can automatically dispense eye drops using a dedicated device (not shown in the diagram) (for example, a commercially available product: product name "Autopipette (product name) (manufactured by ICOMS LABO Co., Ltd.)"). When anti-aging eye drops are administered using an eye drop container, they are effective for reducing intraocular pressure and protecting the retina in age-related macular degeneration, retinitis pigmentosa, dry eye, and glaucoma. The second embodiment shown in Figures 5 and 6 is the same as the embodiments in Figures 1 to 4, except for the eye drop container device 7. In the third embodiment shown in Figures 5 and 6, redundant explanations of parts similar to the first and second embodiments are omitted. Furthermore, in Figure 5, which shows a block diagram of the third embodiment of the mixing system 100-2, components similar to those in the mixing systems 100 and 100-1 of the embodiments shown in Figures 1 to 4 are denoted by the same reference numerals. 【0042】 The procedure for manufacturing an anti-aging agent used as eye drops, using the mixing system 100-2 shown in Figure 5, will be explained primarily with reference to Figure 6. Each of steps S12 to S21 in Figure 6 corresponds to steps S1 to S10 in Figures 2 and 4, so redundant explanations are omitted. In other words, the flowchart in Figure 4 will explain steps that differ from those in the flowcharts in Figures 2 and 4. In step S22 of Figure 4, the raw material (raw material that has undergone mechanotransduction), which has been filtered by filter 5 in step S21 to remove the nuclei of stem cells and mitochondria, is dispensed into eye drop containers by eye drop container dispensing device 7 as an anti-aging agent (eye drop container dispensing process). The other configurations and effects in the third embodiment shown in Figures 5 and 6 are the same as those in the first embodiment shown in Figures 1 to 4. 【0043】 A fourth embodiment of the present invention will be described with reference to Figures 7 and 8. In the fourth embodiment, a mixture of raw material (raw material from which mechanotransduction has occurred) from which the nucleus and mitochondria of stem cells, etc., have been removed by filter 5, and a liquid, is mixed with mineral water containing magnesium, potassium, calcium, and sodium. In Figure 5, a mixer 8 (mixing device) is installed in the path R7 that connects the filter 5 to the storage facility 110. The mineral source 16 has the function of supplying mineral water containing trace elements, and this mineral water is supplied to the mixer 8 via the path R8. In mixer 8, the solution filtered by filter 5 (raw material from which mechanotransduction has occurred, with the nucleus and mitochondria of stem cells etc. removed) is mixed with mineral water containing trace elements. Here, the mineral water is mainly groundwater and contains a large amount of minerals. The amount of mineral water to add is 10 mL of mineral water for every 1 mL to 2 mL of the filtered solution (raw material from which mechanotransduction has occurred, and from which the nucleus of stem cells, etc., and mitochondria have been removed). The trace elements (or minerals) contained in mineral water include, for example, magnesium, potassium, calcium, and sodium. For example, the content per 10 mL of mineral water is 2.7 to 3.0 mg (preferably 2.9 mg) of magnesium, 1.2 to 1.5 mg (preferably 1.3 mg) of potassium, 1.1 to 1.4 mg (preferably 1.3 mg) of calcium, and 27 to 29 mg (preferably 28 mg) of sodium. 【0044】 By including magnesium, potassium, calcium, and sodium in the amounts described above, the solution mixed with mineral water (the raw material from which mechanotransduction has occurred, from which the nucleus and mitochondria of stem cells, etc., have been removed) reaches a mineral concentration similar to that in extracellular fluid, thus increasing its permeability into cells. Therefore, the inventor's experiments have shown that when administered as an anti-aging agent, it penetrates into the user's cells, leading to effective regeneration of stem cells. Cell membranes contain ion channels, including magnesium channels, calcium channels, and potassium channels. At the concentrations mentioned above, the substances within the stem cells, which have been broken down to molecular levels, penetrate into the cells of the administered user through these channels. In the case of calcium, if the content is too high, it can form calcium crystals, which may cause conditions such as cerebral infarction and high blood pressure. 【0045】 In another experiment conducted by the inventor, it was confirmed that penetration into the user's cells deteriorated whether the amount of mineral water added was too large (less than 1 mL of the raw material from which the nuclei of stem cells and mitochondria had been removed per 10 mL of mineral water) or too small (more than 2 mL of the raw material from which the nuclei of stem cells and mitochondria had been removed per 10 mL of mineral water). In the mixer 8, the raw materials (stem cells, etc.) mixed with mineral water containing trace elements are supplied to the storage facility 110 via the pathway R7. Regarding Misaki, publicly known and commercially available products are applicable. 【0046】 The inventor mixed adipose-derived stem cells and pure water in the mixing system shown in Figure 7, and added 10 mL of mineral water to 1.5 mL of the raw material from which the stem cell nuclei and mitochondria had been removed. The 10 mL of mineral water was adjusted to contain 2.9 mg of magnesium, 1.3 mg of potassium, 1.3 mg of calcium, and 28 mg of sodium. Twenty subjects were included in the study. Ten of them received a nasal spray containing the aforementioned mineral water twice a day, morning and evening, for three months, into both nostrils. The remaining ten subjects were administered a mixture prepared using the mixing system 100 shown in Figure 1, but without the addition of mineral water. In this case as well, the mixing ratio of stem cell-derived cells to pure water was 1 mL of pure water per 1 million adipose-derived stem cells. In 10 subjects who were administered a mixture with added mineral water, the ratio of triiodothyronine (T3) to reverse triiodothyronine (rT3), "T3 / rT3," increased by an average of 2.0 to 3.0 points compared to 10 subjects who were administered a mixture without added mineral water. In other words, it was confirmed that the mixture with added mineral water improved hormone regulation ability compared to the mixture without added mineral water. 【0047】 In other experiments conducted by the inventor, it was confirmed that when non-fat-derived stem cells and pure water were mixed using the mixing system shown in Figure 7, and the aforementioned mineral water was added, the mixture with added mineral water exhibited improved hormone regulation ability compared to the mixture without the mineral water. Experiments by other inventors have shown that when a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells is mixed with pure water using the mixing system 100 shown in Figure 1, the hormone regulation ability is also improved. Similar experiments by the inventors have confirmed that when each of the following—adipose-derived or other stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells—is mixed with physiological saline using the mixing system 100 shown in Figure 1, the addition of the aforementioned mineral water improves the hormone-regulating ability. 【0048】 The fourth embodiment shown in Figures 7 and 8 is the same as the embodiments in Figures 1 to 6, except that the mixer 8 (mixing device) mixes the solution filtered by the filter 5 (raw material from which mechanotransduction occurred) with mineral water containing trace elements. Therefore, redundant explanations of parts that are the same as those in the embodiments in Figures 1 to 6 will be omitted. Furthermore, in Figure 7, which shows a block diagram of the mixing system 100-3 of the fourth embodiment, the same reference numerals are used for components similar to those in Figures 1, 3, and 5. 【0049】 The mixing procedure in the anti-aging agent mixing system 100-2 shown in Figure 7 will be explained primarily with reference to Figure 8. Steps S23-S32 and S34 in Figure 8 correspond to steps S1-S11 in Figure 2. Therefore, the flowchart in Figure 6 describes different steps than those in the flowchart in Figure 2. In Figure 8, in step S33, the solution filtered by filter 5 in step S32 (a mixture of raw material from which mechanotransduction has occurred, from which the nucleus of stem cells, etc., and mitochondria have been removed, and liquid) is mixed with mineral water containing trace elements (mainly groundwater as the water source, containing magnesium, potassium, calcium, and sodium) by mixer 8 (mixing device) (mixing step). The mixing procedure of the fourth embodiment shown in Figure 8 is characterized by the addition of step S33 compared to the procedure of the first embodiment shown in Figure 2. Then, in step S34 of Figure 6, the stem cells and other materials (the raw materials filtered in step S32) that were mixed with the mineral water in step S33 are stored in the storage facility 110, where further processing takes place. The subsequent processing is the same as described with reference to Figure 1. 【0050】 Although not shown in Figures 7 and 8, in the fourth embodiment, instead of mixing with mineral water containing trace elements and storing it in the storage facility 110, it can be supplied to the nasal spray dispenser 6 (Figure 3). Alternatively, instead of storing it in the storage facility 110, it can be supplied to the eye drop container dispensing device 7 (Figure 5). The other configurations and effects in the fourth embodiment shown in Figures 7 and 8 are the same as those in the embodiments shown in Figures 1 to 6. 【0051】 The mixing system and mixing method of the present invention allow for the mixing of stem cells that have undergone mechanotransduction, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, and a mixture of stem cells, nerve cells, and immune cells with a liquid (pure water or physiological saline) to obtain test specimens for conducting experiments to verify the effects of beneficial substances obtained through mechanotransduction, without the need for separate dilution. Furthermore, by using the mixing system and mixing method of the present invention, it is possible to improve hormone regulation ability and produce an anti-aging agent that contains substances within the cell membrane of stem cells where mechanotransduction has occurred (inside the region covered by the cell membrane: the region surrounded by the cell membrane), which do not include the nucleus and mitochondria. Furthermore, it is possible to manufacture anti-aging agents containing substances within the cell membranes of stem cells and nerve cells that have undergone mechanotransduction, but which do not include the nucleus and mitochondria. Alternatively, an anti-aging agent can be manufactured that contains substances within the cell membranes of stem cells and immune cells that have undergone mechanotransduction, but which do not include the nucleus and mitochondria. Furthermore, an anti-aging agent can be manufactured that contains substances within the cell membranes of stem cells, nerve cells, and immune cells in which mechanotransduction has occurred, but which do not include the nucleus and mitochondria. Such anti-aging agents preferably contain mineral water containing magnesium, potassium, calcium, and sodium. 【0052】 The illustrated embodiments are for illustrative purposes only and are not intended to limit the technical scope of the present invention. [Explanation of Symbols] 【0053】 1...Raw material freezing equipment 2. Pressurizing device (first pressurizing device) 3...Cooling device 4. Pressure loss mechanism (nozzle, orifice, etc.) 5. Filter 6. Nasal spray dispensing device 7. Eye drop container dispensing device 8. Mixer (mixing device) 9... Raw material injection mechanism 10...Raw material supply source 11...Liquid supply source 12. Temporary storage device 100, 100-1, 100-2, 100-3... Mixed system 110... Storage facilities

Claims

[Claim 1] In a mixing system that mixes stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline, A raw material source that supplies raw materials consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells and immune cells, A liquid supply source that supplies pure water at a rate of 0.7 mL to 1.3 mL per 1 million stem cells, or physiological saline at a rate of 0.8 mL to 1.5 mL per 1 million stem cells, To induce mechanotransduction in stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells, and immune cells, a raw material consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells, and immune cells, and a pressurizing device that pressurizes a liquid, either pure water or physiological saline, to 25 to 200 MPa, A pressure loss mechanism through which the raw material and liquid, pressurized by the pressurizing device, pass and which generates pressure loss during passage, The system includes a cooling device for cooling the raw material and the liquid and / or the pressure drop mechanism that are pressurized by the pressurizing device, A filter with a coarseness of 0.2 μm to 0.3 μm is provided to remove nuclei and mitochondria from the raw material that has passed through the pressure drop mechanism, It has a temporary storage device provided on the pressure loss mechanism side of the filter, which reduces the pressure of the raw material that has passed through the pressure loss mechanism, A mixing system characterized by having a three-way valve provided in the region between the pressure loss mechanism and the temporary storage device, a path connecting the three-way valve and the inlet side of the pressurizing device, and a control unit that determines whether the number of pressurizations and pressure losses on the raw material is a predetermined number, in order to cause mechanotransduction in the raw material by pressurizing it multiple times and causing pressure loss over multiple cycles. [Claim 2] A mineral water source that supplies mineral water containing any of magnesium, potassium, calcium, or sodium, wherein 10 mL of the mineral water contains any of 2.7 to 3.0 mg of magnesium, 1.2 to 1.5 mg of potassium, 1.1 to 1.4 mg of calcium, or 27 to 29 mg of sodium. The mixing system according to claim 1, comprising a mixing device for mixing 10 mL of mineral water with 1 mL to 2 mL of a mixture of the raw material and the liquid that has passed through the filter. [Claim 3] A mixing system according to either claim 1 or 2, comprising a nasal spray dispensing device for dispensing a mixture of the raw material and the liquid that has passed through a filter into a nasal spray. [Claim 4] A mixing system according to either claim 1 or 2, comprising an eye drop container dispensing device for dispensing a mixture of the raw material and the liquid that has passed through a filter into an eye drop container. [Claim 5] In a method for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline, A process of supplying raw materials from a raw material source, consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells and immune cells, A process of supplying pure water from a liquid source at a rate of 0.7 mL to 1.3 mL per 1 million stem cells, or supplying physiological saline at a rate of 0.8 mL to 1.5 mL per 1 million stem cells, A process to induce mechanotransduction in stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells by pressurizing a liquid, either pure water or physiological saline, with a raw material consisting of stem cells, a mixture of stem cells and nerve cells, a mixture of stem cells and immune cells, or a mixture of stem cells, nerve cells and immune cells, to 25 to 200 MPa using a pressurizing device. The process includes a pressure loss step in which pressure loss occurs in the raw material and liquid pressurized by the pressurizing device by passing through a pressure loss mechanism, In the pressurization step and / or the pressure drop step, a cooling step is performed to cool the pressurized raw material, the liquid, and / or the pressure drop mechanism. A filtration step in which nuclei and mitochondria are removed from the raw material that has suffered pressure loss in the pressure loss step by using a filter with a coarseness capable of filtering to a particle size of 0.2 μm to 0.3 μm, On the side of the pressure drop mechanism beyond the filter, there is a step of reducing the pressure of the raw material that has passed through the pressure drop mechanism using a temporary storage device. A mixing method characterized by having a step of generating mechanotransduction in the raw material multiple times by using a three-way valve provided in the region between the pressure drop mechanism and the temporary storage device, a path connecting the three-way valve and the inlet side of the pressurizing device, and a control unit that determines whether the number of times pressurizing and pressure drop on the raw material is a predetermined number. [Claim 6] The method for producing the present invention of claim 5, comprising the step of mixing 1 mL to 2 mL of the mixture of the raw material and the liquid that has passed through the filter with 10 mL of mineral water using a mixing device, wherein the mineral contained in the mineral water is one of magnesium, potassium, calcium, or sodium, and per 10 mL of the mineral water, the amount of magnesium is 2.7 to 3.0 mg, the amount of potassium is 1.2 to 1.5 mg, the amount of calcium is 1.1 to 1.4 mg, or the amount of sodium is 27 to 29 mg. [Claim 7] A mixing method according to either claim 5 or 6, comprising a nasal spray dispensing device for dispensing a mixture of the raw material and the liquid that has passed through a filter into a nasal spray. [Claim 8] A mixing method according to either claim 5 or 6, further comprising a step of dispensing the mixture of the raw material and the liquid obtained from the filtration step into an eye drop container.

Images