A spotting positioning device for cell culture multiwell plates

Abstract

The utility model provides a kind of spotting positioning device for cell culture multi-well plate, the spotting positioning device includes the lower layer bottom plate and upper layer spotting positioning rectangular frame of parallel arrangement;The lower layer bottom plate is also equipped with and is used for supporting the support column of spotting positioning rectangular frame;The top surface of the lower layer bottom plate is formed with the multi-well plate limiting slot for placing multi-well plate;The upper layer spotting positioning rectangular frame is formed with rectangular work window;The spotting positioning device further includes replaceable spotting positioning strip, the spotting positioning strip is formed with several array spotting holes, the spotting positioning strip is erected on the upper layer spotting positioning rectangular frame so that spotting hole is located in rectangular work window;Positioning scale for identifying or assisting positioning spotting positioning strip position is equipped on the upper layer spotting positioning rectangular frame.The spotting positioning device of the application is simple and easy to operate, low in cost, and has high parallelism between multiple holes, suitable for general laboratory applications that do not require excessive samples and have low budgets.

Description

Technical Field

[0001] This utility model belongs to the field of cell culture technology and relates to a spotting and positioning device for multi-well plates used in cell culture. Background Technology

[0002] Organoids are small, self-organized three-dimensional cell cultures derived from stem cells or primary tissues or organs. They have the ability to replicate the complexity of organs and can simulate the physiological functions and pathological features of real organs. Therefore, they can be used as experimental and preclinical research models.

[0003] Currently, organoid-based in vitro drug screening has become a mainstream trend, bringing significant convenience and hope to new drug development. Automated organoid spotting equipment, due to its advantages of full autonomy, high precision, and high throughput, is widely used in drug screening and analysis testing by pharmaceutical companies. Devices such as Corning's Matribot bioprinter, Biogen's Organoid Bioprinter, Agilent's Bio TEK Multiflo FX automated pipetting system, and Meigu Instruments' CellXpress.ai can all perform automated organoid spotting in wells of different sizes as needed, achieving fully autonomous automated organoid drug screening operations. This large-scale organoid system operation is ideal for companies to conduct high-throughput drug detection and screening in the later stages of applied research, making experimental results more reliable and reproducible.

[0004] However, in research-oriented or early-stage exploratory experiments investigating organoid-related drug mechanisms, the actual sample size required is not large. Even in the early stages of testing with high-throughput equipment, only a small number of samples are needed for parameter adjustments. Therefore, fully automated, large-scale operations are unnecessary at this stage, and such equipment is expensive, making it unaffordable for most laboratories. This stage typically involves manual sample loading. However, in traditional organoid culture, the small wells of cell culture plates (e.g., 48-well, 96-well plates) require a small sample volume, making sample loading difficult to control. Furthermore, manual sample loading involves a large number of samples, and the operation is prone to problems such as inconsistent droplet size and uneven distribution between wells, leading to significant errors between replicates after drug testing.

[0005] Therefore, how to provide an organoid artificial spotting auxiliary device that is easy to operate, low in cost, improves the parallelism between duplicate wells, and is suitable for general laboratories is an urgent problem to be solved.

[0006] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solutions of this application and facilitating understanding by those skilled in the art. It should not be assumed that these technical solutions are known to those skilled in the art simply because they have been described in the background section of this application. Utility Model Content

[0007] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a spotting and positioning device for cell culture multi-well plates, so as to solve the problems of high cost of organoid automatic spotting equipment, large error of manual spotting, and large error between replicates after drug testing.

[0008] To achieve the above objectives, this utility model provides a spotting and positioning device for multi-well plates used in cell culture. The spotting and positioning device includes a lower base plate and an upper spotting and positioning rectangular frame arranged in parallel. The lower base plate is also provided with support columns for supporting the spotting and positioning rectangular frame. The top surface of the lower base plate forms a multi-well plate limiting groove for placing the multi-well plate. The upper spotting and positioning rectangular frame forms a rectangular working window. The spotting and positioning device also includes a replaceable spotting and positioning strip, which forms a plurality of arrayed spotting holes. The spotting and positioning strip is mounted on the upper spotting and positioning rectangular frame so that the spotting holes are located within the rectangular working window. The upper spotting and positioning rectangular frame is provided with positioning scales for identifying or assisting in positioning the spotting and positioning strip.

[0009] In one specific implementation, any of the support columns is simultaneously perpendicular to both the lower base plate and the upper point-positioning rectangular frame.

[0010] In one specific embodiment, the perforated plate limiting groove is a rectangular groove with an opening on one side.

[0011] In a more specific embodiment, the sampling positioning device further includes a perforated plate limiting member for locking or unlocking the perforated plate, and a perforated plate limiting member receiving groove communicating with the rectangular groove is formed on the lower bottom plate near the opening of the rectangular groove. The perforated plate limiting member receiving groove is used to place the perforated plate limiting member.

[0012] In a further embodiment, the perforated plate limiting member is a limiting plate disposed parallel to the lower bottom plate, the limiting plate having a perforated plate abutting portion for abutting a corner of the perforated plate; a first adjusting hole is formed on the limiting plate for fixing the limiting plate to the lower bottom plate by passing a bolt through it.

[0013] In a further embodiment, the perforated plate retainer also has a protruding handle.

[0014] In a further embodiment, the perforated plate limiting member further includes a first bolt, and a second threaded section matching the first bolt is formed on the lower base plate, the first bolt being disposed through the first adjusting hole.

[0015] In one specific embodiment, the upper dot-matrix positioning rectangle has stepped frames on opposite sides and includes an outer frame portion and an inner recessed frame portion; the outer frame portion has positioning scales formed on it, and the inner recessed frame portion is used for mounting the dot-matrix positioning strip and for it to abut against the outer frame portion.

[0016] In a more specific embodiment, a strip-shaped hole is formed between the outer frame portion and the inner recessed frame portion. Fixing holes are formed at the corresponding positions of the strip-shaped hole at both ends of the dot-spot positioning strip to allow fixing nails to pass through and fix the dot-spot positioning strip to the upper dot-spot positioning rectangular frame. The fixing nails slide in the strip-shaped hole to drive the dot-spot positioning strip to move back and forth along the direction of the strip-shaped hole.

[0017] In a further embodiment, the fixing pin includes a third bolt and a matching second nut, the third bolt being disposed through the strip-shaped hole.

[0018] In a more specific embodiment, the dotted positioning strip has a downwardly protruding limiting portion that engages between two opposing inner recessed frame portions.

[0019] In one specific implementation, the size and spacing of the sampling holes are consistent with the size and spacing of the holes in each row of the perforated plate.

[0020] In one specific implementation, the upper-level dot-matrix positioning rectangle is detachably connected to the support column.

[0021] In one specific embodiment, the sampling positioning device further includes several protective posts, which are disposed on the lower base plate corresponding to the upper sampling positioning rectangle and are used to prevent the upper sampling positioning rectangle from falling.

[0022] In a more specific embodiment, a support portion is formed at one end of the protective column near the upper-level dot-matrix positioning rectangle, and a support surface parallel to the lower-level base plate is formed at the top of the support portion.

[0023] In a more specific embodiment, an interference-fit hole is formed on the lower base plate corresponding to the protective post for the protective post to pass through, and the protective post is formed with a first threaded section. The spotting positioning device further includes a first nut that matches the first threaded section. The first nut matches the first threaded section to control the height of the protective post protruding from the lower base plate.

[0024] In one specific embodiment, the upper sampling positioning rectangle is provided with a through hole for the support column to pass through at the corresponding position of the support column. The upper sampling positioning rectangle also includes a second bolt, and the upper sampling positioning rectangle is also formed with a positioning through hole for the second bolt to enter and abut against the support column from the side. The positioning through hole is formed with a third threaded section that matches the second bolt.

[0025] In a more specific embodiment, the support column further includes an abutment groove for the second bolt to abut.

[0026] In one specific embodiment, the support column also includes a height scale.

[0027] In a more specific embodiment, the upper dot-matrix positioning rectangle is further provided with several height indicators. The height indicators are "L"-shaped and consist of two extensions. One extension is fixedly connected to the upper dot-matrix positioning rectangle, and the free end of the other extension extends to indicate the height at the height scale.

[0028] In one specific embodiment, the sampling positioning strip includes a first positioning strip that matches the hole positions of a 48-well multi-well plate and a second positioning strip that matches the hole positions of a 96-well multi-well plate; the first positioning strip and the second positioning strip include one or more rows of sampling holes.

[0029] In one specific embodiment, the positioning scale includes a first positioning scale that matches the row spacing of the 48-hole perforated plate and a second positioning scale that matches the row spacing of the 96-hole perforated plate.

[0030] As described above, the sample placement device for multi-well plates used in cell culture according to this utility model has the following beneficial effects:

[0031] 1) In this application, the position of the spotting positioning strip can be adjusted to adapt to various models of multi-well plates, and the spotting wells are matched with the well positions of the multi-well plates, so that the spotting operation can be performed at the center point of each well of the cell culture multi-well plate, thereby reducing experimental errors between replicates.

[0032] 2) In this application, the height of the upper sampling positioning frame is adjustable and indicated, so that different experimental personnel can adjust the upper sampling positioning frame according to their personal habits and the characteristics of the sample itself, and further adjust the sampling height to ensure the consistency of multiple batches of sampling.

[0033] 3) The spotting and positioning device described in this application is easy to operate and inexpensive, and is suitable for application scenarios in ordinary laboratories that do not require a large number of samples and have a low budget. Attached Figure Description

[0034] Figure 1The diagram shown is a three-dimensional structural schematic of the sampling and positioning device provided by this utility model.

[0035] Figure 2 The diagram shown is a top view of the lower base plate of the sampling and positioning device provided by this utility model.

[0036] Figure 3 The diagram shown is a top view of the perforated plate limiting component in the sampling positioning device provided by this utility model.

[0037] Figure 4 The diagram shown is a top view of the upper sampling positioning rectangle in the sampling positioning device provided by this utility model.

[0038] Figure 5 The diagram shown is a side view of the upper sampling positioning rectangle in the sampling positioning device provided by this utility model.

[0039] Figure 6 The diagram shows the front and top views of the first tube sleeve in the sampling and positioning device provided by this utility model.

[0040] Figure 7 The diagram shows the front and top views of the second tube sleeve in the sampling and positioning device provided by this utility model.

[0041] Figure 8 The diagram shown is a structural schematic of the support column in the sampling positioning device provided by this utility model.

[0042] Explanation of reference numerals in the attached figures

[0043] 10 Lower base plate, 11 Perforated plate limiting groove, 12 Protective post, 121 First nut, 122 Support part, 13 Perforated plate limiting part, 131 Protruding handle, 132 Limiting plate, 1321 First adjusting hole, 133 First bolt, 134 Perforated plate supporting part, 14 Interference through hole, 20 Support post, 21 Abutment groove, 22 Height scale, 30 Upper sampling positioning rectangle, 31 Second bolt, 32 Height indicator, 33 Third bolt, 34 Second positioning scale, 35 First positioning scale, 36 Strip hole, 40 First positioning strip, 41 First sampling hole, 50 Second positioning strip, 51 Second sampling hole, 60 First tube sleeve strip, 61 First tube sleeve, 70 Second tube sleeve strip, 71 Second tube sleeve, 80 Perforated plate. Detailed Implementation

[0044] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model.

[0045] Please see Figures 1-8 This embodiment provides a sample placement device for a multi-well plate used in cell culture. It should be noted that the structures, proportions, and sizes illustrated in the accompanying drawings are merely for illustrative purposes and to aid those skilled in the art; they are not intended to limit the scope of this invention and therefore have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to the size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, in this application, "lower" refers to the side closest to the abrasive pad. Terms such as "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0046] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0047] See Figures 1-8This embodiment provides a spotting positioning device for a cell culture multiwell plate. The spotting positioning device includes a lower base plate 10 and an upper spotting positioning rectangular frame 30 arranged in parallel. The lower base plate 10 is also provided with a support column 20 for supporting the spotting positioning rectangular frame 30. The top surface of the lower base plate 10 forms a multiwell plate limiting groove 11 for placing the multiwell plate 80. The upper spotting positioning rectangular frame 30 forms a rectangular working window. The spotting positioning device also includes a replaceable spotting positioning strip, which forms a plurality of arrayed spotting holes. The spotting positioning strip is mounted on the upper spotting positioning rectangular frame 30 so that the spotting holes are located within the rectangular working window. The upper spotting positioning rectangular frame 30 is provided with positioning scales for identifying or assisting in positioning the spotting positioning strip. The sampling positioning strip is mounted on the upper sampling positioning rectangle 30. The position of the sampling positioning strip is identified by a positioning scale or assisted in positioning, ensuring that its sampling holes are within the rectangular working window and correspond to the holes in the multi-well plate 80. This allows the sampling positioning device to apply samples to the center point of each hole, thereby reducing experimental errors between replicates. The sampling positioning device described in this application is simple to operate, low in cost, and suitable for applications in ordinary laboratories that do not require a large number of samples and have a low budget.

[0048] In one specific embodiment, the multi-well cell culture plate includes one or more of the following: 6-well multi-well plate, 12-well multi-well plate, 24-well multi-well plate, 48-well multi-well plate, and 96-well multi-well plate. It can be from different brands and manufacturers, such as Thermo Fisher Scientific Nunclon series multi-well cell plates, Corning Costar series multi-well cell plates, and Greiner Bio-One multi-well cell plates. The multi-well cell culture plates from the above different manufacturers all include 6-well, 12-well, 24-well, 48-well, and 96-well plates.

[0049] It should be noted that the dimensions of the cell culture multiwell plate described in this application are general standard dimensions, conforming to the ANSI / SLAS standard, that is, the length of the cell culture multiwell plate is 127.76 mm, the width is 85.46 mm, and the height is 16-22 mm (including the cap).

[0050] In a more specific embodiment, such as Figures 1-8 As shown, the porous plate is selected from one of a 48-hole porous plate and a 96-hole porous plate.

[0051] In a like Figures 1-2 In the specific embodiment shown, the porous plate 80 is a 96-hole porous plate.

[0052] The sample placement device described in this application can be a metal sample placement device, a plastic sample placement device, or a ceramic sample placement device. The sample placement device described in this application has low manufacturing difficulty and cost. In a... Figures 1-8 In the specific embodiment shown, the sampling positioning device is a plastic sampling positioning device.

[0053] In a like Figures 1-2 In the specific embodiment shown, the lower base plate 10 is rectangular in shape.

[0054] It should be noted that the size and shape of the base plate in this application can be arbitrarily selected according to actual needs, as long as it can form the perforated plate limiting groove 11.

[0055] In a specific embodiment, such as Figure 1 , 2 As shown in Figure 4, any of the support columns 20 is simultaneously perpendicular to the lower base plate 10 and the upper point-positioning rectangular frame 30.

[0056] In a specific embodiment, such as Figures 1-2 As shown, the perforated plate limiting groove is a rectangular groove with an opening on one side. The perforated plate limiting groove 11 allows the perforated plate to be inserted into the rectangular groove from the opening, thereby limiting the perforated plate.

[0057] In a specific embodiment, such as Figures 1-2 As shown, the single-side opening of the perforated plate limiting groove 11 can be either a long side opening or a wide side opening.

[0058] In a like Figures 1-2 In the specific embodiment shown, the opening on one side of the perforated plate limiting groove 11 is a wide-side opening.

[0059] In a specific embodiment, such as Figures 1-2 As shown, the perforated plate limiting groove 11 is a rectangular groove with a width of 85.5–87 mm, a length of 127.8–250 mm, and a depth of 8–20 mm. This allows the perforated plate 80 to be inserted into the perforated plate limiting groove 11 from the wide-side opening, and limits the movement of the perforated plate 80 in the longitudinal direction. In this application, the longitudinal direction refers to the direction from one long side of the rectangular groove to the other long side.

[0060] In a like Figures 1-2 In the specific embodiment shown, the perforated plate limiting groove 11 has a width of 85.5 mm, a length of 127.8 mm, and a depth of 10 mm.

[0061] In one specific embodiment, the number of support columns 20 is 1 to 8. For example, it can be 1, 2, 3, 4, 5, 6, 7, or 8.

[0062] It should be noted that the number, position, diameter and length of the support columns 20 can be selected according to actual needs, as long as they can stably support the upper point-positioning rectangular frame 30.

[0063] In a like Figures 1-2 In the specific embodiment shown, the number of support columns 20 is 4, and the support columns 20 are respectively located at the four corners of the lower base plate 10.

[0064] In a more specific embodiment, such as Figures 1-3 As shown, the sampling positioning device further includes a perforated plate limiting member 13 for locking or unlocking the perforated plate 80. A perforated plate limiting member receiving groove, communicating with the rectangular groove, is also formed on the lower bottom plate 10 near the opening of the rectangular groove. The perforated plate limiting member receiving groove is used to place the perforated plate limiting member 13. The perforated plate limiting member 13 can move back and forth within the perforated plate limiting member receiving groove.

[0065] In a further embodiment, the perforated plate limiting member 13 is a limiting plate 132 disposed parallel to the lower base plate 10. The limiting plate 132 has a perforated plate abutting portion 134 for abutting one corner of the perforated plate 80. A first adjusting hole 1321 is formed on the limiting plate 132 for fixing the limiting plate 13 to the lower base plate 10 by passing a bolt through it. The perforated plate abutting portion 134 abuts one corner of the perforated plate 80, thereby fixing the perforated plate 80 in both the lateral and longitudinal positions. After the perforated plate limiting member receiving groove moves back and forth until the perforated plate abutting portion 134 abuts one corner of the perforated plate 80, the perforated plate limiting member 13 is fixed to the lower base plate 10 by a bolt passing through the first adjusting hole 1321.

[0066] In a further embodiment, such as Figures 1-3 As shown, the perforated plate limiting member 13 also has a protruding handle 131. The protruding handle 131 makes the perforated plate limiting member 13 easy to adjust and move.

[0067] In a further embodiment, such as Figures 1-3 As shown, the perforated plate limiting member 13 also includes a first bolt 133, and a second threaded section matching the first bolt 133 is formed on the lower base plate 10. The first bolt 133 is disposed through the first adjusting hole 1321.

[0068] In a specific embodiment, such as Figures 1-5As shown, the upper-layer dot-matrix positioning rectangle 30 has stepped frames on both sides, including an outer frame portion and an inner recessed frame portion. Positioning scales are formed on the outer frame portion, and the inner recessed frame portion is used for mounting the dot-matrix positioning strip and for it to abut against the outer frame portion. The dot-matrix positioning strip is mounted and abuts against the outer frame portion, so that the upper surface of the dot-matrix positioning strip and the upper surface of the outer frame portion are on the same plane, thereby better aligning the positioning scales with the dot-matrix positioning strip to achieve auxiliary positioning.

[0069] In a more specific embodiment, such as Figures 1-5 As shown, a strip-shaped hole 36 is formed between the outer frame portion and the inner recessed frame portion. Fixing holes are formed at the corresponding positions of the strip-shaped hole 36 at both ends of the dot-shaped positioning strip to allow fixing nails to pass through and fix the dot-shaped positioning strip to the upper dot-shaped positioning rectangular frame 30. The fixing nails slide in the strip-shaped hole 36 to drive the dot-shaped positioning strip to move back and forth along the direction of the strip-shaped hole 36.

[0070] In a further embodiment, such as Figures 1-5 As shown, the fixing pin includes a third bolt 33 and a matching second nut, wherein the third bolt 33 is disposed through the strip-shaped hole 36.

[0071] In a more specific embodiment, such as Figures 1-5 As shown, the dotted positioning strip has a downwardly protruding limiting part, which is engaged between two opposing inner recessed frame parts.

[0072] In a specific embodiment, such as Figures 1-5 As shown, the size and spacing of the sampling holes are consistent with the size and spacing of the holes in each row of the perforated plate.

[0073] In a specific embodiment, such as Figures 1-2 As shown, the upper-level dot-matrix positioning rectangle 30 is detachably connected to the support column 20.

[0074] In a specific embodiment, such as Figures 1-2 As shown, the sampling positioning device also includes several protective posts 12. These protective posts 12 are positioned on the lower base plate 10 at the corresponding locations of the upper sampling positioning rectangle 30, and are used to prevent the upper sampling positioning rectangle 30 from falling. The protective posts 12 ensure that when adjusting the height of the upper sampling positioning rectangle 30, it will not directly slide onto the lower base plate 10, thus improving the ease of operation and stability of the device.

[0075] In a more specific embodiment, such as Figures 1-2As shown, a support portion 122 is formed at one end of the protective column 12 near the upper sampling positioning rectangle 30, and a support surface parallel to the lower base plate 10 is formed at the top of the support portion 122. The support surface is parallel to the lower base plate 10 to ensure that the upper sampling positioning rectangle 30 is placed stably.

[0076] In a further embodiment, such as Figures 1-2 As shown, the shape of the support part 122 is selected from one or more of cylinders and cubes. It should be noted that the shape of the support part 122 can be arbitrarily selected according to actual needs, as long as it can stably support the upper point-positioning rectangular frame 30.

[0077] In a more specific embodiment, such as Figures 1-2 As shown, an interference hole 14 is formed on the lower base plate 10 corresponding to the protective post 12 for the protective post 12 to pass through, and the protective post 12 is formed with a first threaded section. The spotting positioning device also includes a first nut 121 that matches the first threaded section. The first nut 121 matches the first threaded section to control the height of the protective post 12 protruding from the lower base plate 10.

[0078] In a like Figures 1-2 In the specific embodiment shown, the number of protective pillars 12 is 2. It should be noted that the number of protective pillars 12 can be selected according to actual needs, such as 1, 2, 3, 4, 5, or 6, as long as they can stably support the upper sampling positioning rectangle 30.

[0079] In a like Figures 1-2 In the specific embodiment shown, the number of interference holes 14 is 6. It should be noted that the number of interference holes 14 can be selected according to actual needs, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, as long as it is greater than the number of protective posts 12.

[0080] In a specific embodiment, such as Figures 1-5 As shown, the upper sampling positioning rectangle 30 is provided with a through hole for the support column 20 to pass through at the corresponding position of the support column 20. The upper sampling positioning rectangle 30 also includes a second bolt 31, and the upper sampling positioning rectangle 30 also forms a positioning through hole for the second bolt 31 to enter and abut against the support column 20 from the side. The positioning through hole forms a third threaded section that matches the second bolt 31.

[0081] In a more specific embodiment, such as Figures 1-5As shown, the support column 20 also includes an abutment groove 21 for the second bolt 31 to abut. After the upper sampling positioning rectangle 30 moves to the required height along the extension direction of the abutment groove 21, the second bolt 31 matches the third threaded section, limiting and fixing the upper sampling positioning rectangle 30 at the specified height.

[0082] In a specific embodiment, such as Figures 1-5 As shown in Figure 8, the support column 20 also includes a height scale 22.

[0083] In a more specific embodiment, such as Figures 1-5 As shown in Figure 8, the upper sampling positioning rectangle 30 is also provided with several height indicators 32. Each height indicator 32 is L-shaped, consisting of two extensions. One extension is fixedly connected to the upper sampling positioning rectangle 30, while the free end of the other extension extends to indicate the height at the height scale 22. One end of the height indicator 32 indicates the height scale 22 to record a fixed height when adjusting the upper sampling positioning rectangle 30. This facilitates controlling the same sampling height in multiple experiments or sampling operations, resulting in more precise and consistent control during multiple sampling processes, improving the parallelism between replicates, and thus reducing experimental errors between replicates.

[0084] In a specific embodiment, such as Figures 1-8 As shown, the sampling positioning strip includes a first positioning strip 40 that matches the hole positions of the 48-well multi-hole plate and a second positioning strip 50 that matches the hole positions of the 96-well multi-hole plate; the first positioning strip 40 and the second positioning strip 50 include one or more rows of sampling holes.

[0085] In a more specific embodiment, such as Figure 1 and 4 As shown in Figure 7, the first positioning strip 40 has a first sampling hole 41, and the size and spacing of the first sampling hole 41 are consistent with the size and spacing of the holes in each row of the 48-hole multi-hole plate.

[0086] In a more specific embodiment, such as Figure 1 and 4 As shown in Figure 7, the second positioning strip 50 has a second sampling hole 51, and the size and spacing of the second sampling hole 51 are consistent with the size and spacing of the holes in each row of the 96-hole multi-hole plate.

[0087] In a specific embodiment, such as Figures 1-8 As shown, the positioning scale includes a first positioning scale 35 that matches the spacing between rows of the 48-hole perforated plate and a second positioning scale 34 that matches the spacing between rows of the 96-hole perforated plate.

[0088] In a more specific embodiment, such as Figure 1 and 4As shown in Figure 7, a first tube sleeve 60 is inserted into the first positioning strip 40. The first tube sleeve 60 forms a plurality of first tube sleeves 61. The position and diameter of the first tube sleeves 61 are consistent with the first sampling hole 41. The size of the first tube sleeves 61 matches the pipetting end of the pipette.

[0089] In a more specific embodiment, such as Figure 1 and 4 As shown in Figure 7, a second tube sleeve 70 is inserted into the second positioning strip 50. The second tube sleeve 70 forms a plurality of second tube sleeves 71. The position and diameter of the second tube sleeves 71 are consistent with the second spotting hole 51. The size of the second tube sleeves 71 matches the pipetting end of the pipette.

[0090] This invention effectively overcomes some shortcomings of the prior art and has high industrial application value.

[0091] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. A sample placement device for multi-well plates used in cell culture, characterized in that, The sampling positioning device includes a lower base plate (10) and an upper sampling positioning rectangular frame (30) arranged in parallel. The lower base plate (10) is also provided with a support column (20) for supporting the sampling positioning rectangular frame. The top surface of the lower base plate (10) is formed with a perforated plate limiting groove (11) for placing a perforated plate. The upper sampling positioning rectangular frame (30) is formed with a rectangular working window. The sampling positioning device also includes a replaceable sampling positioning strip, which is formed with a plurality of arrayed sampling holes. The sampling positioning strip is mounted on the upper sampling positioning rectangular frame (30) so that the sampling holes are located within the rectangular working window. The upper sampling positioning rectangular frame (30) is provided with a positioning scale for identifying or assisting in positioning the sampling positioning strip.

2. The sampling positioning device according to claim 1, characterized in that, Any of the support columns (20) is simultaneously perpendicular to the lower base plate (10) and the upper dotted positioning rectangle (30); And / or, the perforated plate limiting groove (11) is a rectangular groove with an opening on one side; And / or, the upper dot-matrix positioning rectangle (30) has stepped frames on opposite sides, and includes an outer frame portion and an inner recessed frame portion; the outer frame portion has positioning scales formed on it, and the inner recessed frame portion is used for mounting the dot-matrix positioning strip and for it to abut against the outer frame portion. And / or, the size and spacing of the sampling holes are consistent with the size and spacing of the holes in each row of the perforated plate; And / or, the upper dotted positioning rectangle (30) is detachably connected to the support column (20); And / or, the sampling positioning device further includes a plurality of protective columns (12), which are disposed on the lower base plate (10) at the corresponding position of the upper sampling positioning rectangle (30) and are used to prevent the upper sampling positioning rectangle (30) from falling off.

3. The sampling positioning device according to claim 2, characterized in that, A strip-shaped hole (36) is formed between the outer frame and the inner recessed frame. Fixing holes are formed at the corresponding positions of the strip-shaped hole (36) at both ends of the dotted positioning strip so that fixing nails can pass through to fix the dotted positioning strip to the upper dotted positioning rectangular frame (30). The fixing nails slide in the strip-shaped hole (36) to drive the dotted positioning strip to move back and forth along the direction of the strip-shaped hole (36). And / or, the dotted positioning strip has a downwardly protruding limiting part, which is engaged between two opposing inner recessed frame parts; And / or, the spotting positioning device further includes a perforated plate limiting member (13) for locking or unlocking the perforated plate, and a perforated plate limiting member receiving groove communicating with the rectangular groove is also formed on the lower bottom plate (10) near the opening of the rectangular groove, the perforated plate limiting member receiving groove being used to place the perforated plate limiting member (13). And / or, the protective column (12) has a support part (122) formed at one end near the upper point positioning rectangular frame (30), and the top of the support part (122) has a support surface parallel to the lower bottom plate (10); And / or, an interference through hole (14) is formed on the lower base plate (10) corresponding to the protective column (12) for the protective column (12) to pass through, and the protective column (12) is formed with a first threaded section. The spotting positioning device also includes a first nut (121) that matches the first threaded section. The first nut (121) matches the first threaded section to control the height of the protective column (12) protruding from the lower base plate (10).

4. The sampling positioning device according to claim 3, characterized in that, The perforated plate limiting member (13) is a limiting plate (132) arranged parallel to the lower bottom plate (10). The limiting plate (132) has a perforated plate supporting part (134) for supporting one corner of the perforated plate. A first adjusting hole (1321) is formed on the limiting plate (132) for fixing the limiting plate to the lower bottom plate (10) by passing a bolt through it. And / or, the fixing pin includes a third bolt (33) and a matching second nut, the third bolt (33) being disposed through the strip hole (36).

5. The spotting positioning device according to claim 4, characterized in that, The perforated plate limiting member (13) also has a protruding handle (131); And / or, the perforated plate limiting member (13) further includes a first bolt (133), and a second threaded section matching the first bolt (133) is formed on the lower base plate (10), and the first bolt (133) is disposed through the first adjusting hole (1321).

6. The sampling positioning device according to claim 1, characterized in that, The upper-level dot-matrix positioning rectangle (30) is provided with a through hole for the support column (20) to pass through at the corresponding position of the support column (20). The upper-level dot-matrix positioning rectangle (30) also includes a second bolt (31), and the upper-level dot-matrix positioning rectangle (30) also forms a positioning through hole for the second bolt (31) to enter and abut against the support column (20) from the side. The positioning through hole forms a third threaded section that matches the second bolt (31).

7. The sampling positioning device according to claim 6, characterized in that, The support column (20) also includes an abutment groove (21) for the second bolt (31) to abut.

8. The sampling positioning device according to claim 1, characterized in that, The support column (20) also includes a height scale (22); And / or, the spotting positioning strip includes a first positioning strip (40) that matches the hole positions of a 48-well multi-hole plate and a second positioning strip (50) that matches the hole positions of a 96-well multi-hole plate; the first positioning strip (40) and the second positioning strip (50) include one or more rows of spotting holes.

9. The sampling positioning device according to claim 8, characterized in that, The upper dot-matrix positioning rectangle (30) is also provided with several height indicators (32). The height indicator (32) is "L" shaped and consists of two extensions. One extension is fixedly connected to the upper dot-matrix positioning rectangle (30), and the free end of the other extension extends to indicate the height at the height scale (22).

10. The sampling positioning device according to claim 1, characterized in that, The positioning scale includes a first positioning scale (35) that matches the row spacing of the 48-hole perforated plate and a second positioning scale (34) that matches the row spacing of the 96-hole perforated plate.

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