A molecular computing method based on DNA origami technology and application thereof

Abstract

The present application belongs to the technical field of biology, and more particularly, the present application relates to a molecular computing method based on DNA origami technology and application thereof. The molecular computing method provided by the present application performs complex mathematical models by utilizing the addressing ability of DNA origami in positioning components and the programmability of DNA recognition, and the positioning of DNA components avoids the interference of diffusible chains, which is conducive to increasing the complexity of molecular reaction networks and opens up a new way for promoting molecular computing.

Claims

1. A molecular computation method based on DNA origami technology, characterized in that, The method includes the following steps: 1) Obtain preset conditions based on the mathematical problem to be solved, and encode DNA sequences for the DNA origami frame and double-cross tiles respectively according to the preset conditions; 2) Construct the DNA origami framework; 3) Construct the double-cross tiles and mark them; 4) The DNA origami frame and the double-cross tiles are mixed and reacted on a computing platform to obtain the reaction product; 5) The reaction products are detected using a detector to obtain the calculation results; The nucleotide sequences of the double-cross tiles in step 3) are shown in Table 1 or Table 2. Among them, the mathematical problem to be solved in step 1) is a prime factorization problem; The numbers to be decomposed for the mathematical problem to be solved are 6 and 15.

2. The method as described in claim 1, characterized in that, The conditions for the mixing reaction in step 4) are as follows: DNA origami dimers and DX tiles are mixed in 1×TAE-Mg buffer at a molar ratio of 1:20; the mixture is annealed from 45ºC to 30ºC at a rate of -0.1ºC / min, and then incubated at 30ºC for 12h.

3. The method as described in claim 1, characterized in that, The detector in step 5) is an atomic force microscope.

4. A molecular computer based on DNA origami technology, characterized in that, The molecular computer includes: a DNA origami frame and double-cross tiles, wherein the nucleotide sequences of the double-cross tiles are shown in Table 1 or Table 2; The molecular computer mentioned above is used in solving mathematical problems. The mathematical problem to be solved is the prime factorization problem. The numbers to be decomposed for the mathematical problem to be solved are 6 and 15.

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