A crp mutant gene and its use

By introducing CRP mutant genes, especially the CRP mu9 mutant, into Escherichia coli and Pseudomonas putida, the glucose inhibition effect was resolved, glycolysis and the TCA cycle were activated, glucose utilization and biosynthesis were enhanced, strain growth was promoted, and the level of recombinant expression was increased.

CN116284277BActive Publication Date: 2025-11-25INST OF MICROBIOLOGY CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202310234936.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-03-13
Publication Date
2025-11-25
Estimated Expiration
2043-03-13

AI Technical Summary

Technical Problem

In the prior art, Escherichia coli exhibits a glucose inhibition effect in the presence of glucose, leading to a decrease in recombinant expression levels and limited cell growth. In particular, under conditions where glucose is used as a carbon source, commonly used promoters such as lactose promoters are inhibited, affecting biosynthetic capacity.

Method used

By introducing CRP mutant genes, especially the CRP mu9 mutant, the glucose inhibition effect is relieved, glycolysis and the TCA cycle are activated, glucose utilization and biosynthesis are enhanced, and strain growth is promoted.

Benefits of technology

CRP mutant genes, especially the CRP mu9 mutant, significantly relieve glucose inhibition in the presence of glucose, promote strain growth, increase recombinant expression levels, and enhance biosynthetic capacity, particularly in Escherichia coli and Pseudomonas putida.

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Abstract

The application discloses a CRP mutant gene and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the CRP mutant gene is shown in SEQ ID NO. 7 or SEQ ID NO. 8 or SEQ ID NO. 9, the CRP mutant gene can significantly eliminate the 'glucose inhibition' effect, activate glycolysis and TCA cycle in a basic medium with glucose as a carbon source, strengthen the utilization and biosynthesis of glucose, and promote the growth of a strain, and the CRP mutant gene with the amino acid sequence shown in SEQ ID NO. 7, 8 or 9 also has the effect of improving the expression amount of vanillin or naringenin of the strain.
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