Mixing and heating device for nucleic acid extraction and nucleic acid extraction system

Abstract

This invention discloses a mixing and heating device and a nucleic acid extraction system for nucleic acid extraction. By placing the sample mixing module and the sample heating module on the same support, an integrated arrangement of the sample mixing module and the sample heating module is achieved. This not only results in a compact structure, but also allows consumables to be placed on the positioning frame of the sample mixing module, completing both the shaking and heating processes without the need for transfer. This solves the problem of frequent transfer of extracts between the mixing unit and the heating unit, which leads to low experimental efficiency and cross-contamination. The nucleic acid extraction system includes the aforementioned mixing and heating device for nucleic acid extraction. The inclusion of this device helps improve the efficiency of the entire nucleic acid extraction experiment and the reliability of experimental data, making it highly practical.

Description

Technical Field

[0001] This invention belongs to the field of biological research technology, and relates to nucleic acid extraction equipment, particularly to a mixing and heating device and a nucleic acid extraction system for nucleic acid extraction. Background Technology

[0002] Nucleic acids are the basic units representing the genetic characteristics of living organisms. Nucleic acid detection is an advanced biological detection method at the molecular level, offering significant advantages over traditional morphological, cytological, and immunological detection methods, including greater sensitivity, specificity, and the absence of a window period. In recent years, nucleic acid detection has gradually been recognized as a standard gold standard method, widely used in the detection and diagnosis of major infectious diseases such as hepatitis and AIDS, as well as emerging infectious diseases such as SARS, influenza, and hand-foot-mouth disease, and in biological and medical research.

[0003] With the continuous development of molecular diagnostic technology, hospital departments and testing centers have placed higher demands on the automation of equipment. In the past, nucleic acid extraction experiments were mostly carried out using semi-automated equipment, requiring manual assistance. The extraction process was complex and tedious, taking several hours to complete. Furthermore, in commonly available extraction equipment, mixing and heating are often separate unit structures. This not only occupies significant space resources but also requires frequent transfer of the extract between the mixing and heating units, reducing experimental efficiency and increasing the risk of cross-contamination, thus affecting the reliability of the experiment. Summary of the Invention

[0004] The purpose of this invention is to provide a mixing and heating device and a nucleic acid extraction system for nucleic acid extraction, so as to solve the problems of the existing extraction equipment where the mixing unit structure and the heating unit structure are set up independently, which not only occupies a lot of space, but also requires frequent transfer of extracts between the mixing unit structure and the heating unit structure, resulting in low experimental efficiency and easy cross-contamination.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] This invention provides a mixing and heating device for nucleic acid extraction, comprising:

[0007] support;

[0008] The sample mixing module is disposed on the support. The sample mixing module includes a positioning frame and a mixing drive mechanism connected to the positioning frame. The positioning frame is used to place consumables that can hold the extracted sample. The mixing drive mechanism is used to drive the positioning frame to move so that the extracted sample in the consumables is shaken and mixed.

[0009] A sample heating module is mounted on the support and is used to heat the extracted sample inside the consumable.

[0010] Optionally, the consumable is a deep-hole plate, and the positioning frame is provided with positioning slots for the consumable to pass through and fixing components that can fix the deep-hole plate.

[0011] Optionally, the positioning frame is located above the support. The mixing drive mechanism includes a mixing drive motor, an active polarizing rod, and a driven polarizing rod. The positioning frame is connected to the top of the support through one active polarizing rod and at least one driven polarizing rod. The top of the active polarizing rod and any one of the driven polarizing rods are rotatably connected to the positioning frame through bearings. The active polarizing rod and any one of the driven polarizing rods are also rotatably connected to the support through bearings. The mixing drive motor is mounted on the support and connected to the bottom end of the active polarizing rod. The mixing drive motor is used to drive the positioning frame to vibrate relative to the support.

[0012] Optionally, the fastener is a spring-loaded self-resetting buckle.

[0013] Optionally, the sample heating module is located below the positioning frame. The sample heating module includes a heating block, a heating rod disposed on the heating block, and a heating drive mechanism connected to the heating block. The heating block is used to heat the extracted sample in the consumable, and the heating drive mechanism is used to drive the heating block closer to or away from the bottom of the consumable.

[0014] Optionally, the upper part of the heating block is provided with a receiving groove that can be embedded in the bottom of the consumable; multiple heating rods are embedded in the lower part of the heating block; the heating drive mechanism is located below the heating block and is used to drive the heating block to rise and fall relative to the positioning frame so that the receiving groove covers the bottom of the consumable or moves away from the bottom of the consumable.

[0015] Optionally, the heating drive mechanism includes:

[0016] A lifting drive motor is located at the bottom of the bracket;

[0017] A lead screw, the bottom end of which is connected to the lifting drive motor;

[0018] A lead screw nut, wherein the lead screw nut is threadedly connected to the lead screw, and the top of the lead screw nut is connected to the heating block, and the bottom of the heating block is provided with a clearance hole for the lead screw to pass through;

[0019] A guide rod is provided parallel to the lead screw. The top end of the guide rod is connected to the heating block, and the bottom end of the guide rod is slidably engaged with the bracket.

[0020] Optionally, a heat insulation block is also provided at the bottom of the heating block, and the top end of the guide rod is connected to the heat insulation block.

[0021] Optionally, the support includes an upper support, a middle support, and a lower support arranged sequentially from top to bottom, and any adjacent supports are connected by a support column; the positioning frame is disposed above the upper support, and the heating block is disposed between the upper support and the middle support.

[0022] Optionally, it also includes a control module, which is electrically connected to the heating rod, the lifting drive motor and the mixing drive mechanism.

[0023] The present invention also proposes a nucleic acid extraction system, including a nucleic acid extraction device and a mixing and heating device for nucleic acid extraction as described in any of the above.

[0024] The present invention achieves the following technical effects compared to the prior art:

[0025] The nucleic acid extraction mixing and heating device proposed in this invention achieves integrated arrangement of the sample mixing module and the sample heating module by setting them on the same support. This not only results in a compact structure, but also allows the consumables to be placed on the positioning frame of the sample mixing module, completing both the shaking and heating processes without the need for transfer. This solves the problem that currently requires frequent transfer of extracts between the mixing unit and the heating unit, leading to low experimental efficiency and easy cross-contamination.

[0026] In some technical solutions of the present invention, the support is configured as a layered structure, which realizes the layered arrangement of the sample mixing module and the sample heating module on the support. This not only makes the layout reasonable, but also further enhances the structural compactness of the integrated sample heating module and sample mixing module, and improves the space utilization rate.

[0027] The nucleic acid extraction system proposed in this invention includes the above-mentioned mixing and heating device for nucleic acid extraction. The setting of the mixing and heating device for nucleic acid extraction helps to improve the efficiency of the entire nucleic acid extraction experiment and the reliability of experimental data, and is highly practical. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of the overall structure of the mixing and heating device for nucleic acid extraction disclosed in the embodiments of the present invention;

[0030] Figure 2 This is a schematic diagram of the structure of the consumables disclosed in the embodiments of the present invention;

[0031] Figure 3 This is a schematic diagram of the positioning frame in the sample mixing module disclosed in the embodiments of the present invention;

[0032] Figure 4 This is a schematic diagram of the sample mixing module disclosed in the embodiments of the present invention;

[0033] Figure 5 This is a schematic diagram of the sample mixing module disclosed in the embodiment of the present invention, wherein the outer shell of the support has been removed;

[0034] Figure 6 This is a schematic diagram of the sample heating module disclosed in the embodiments of the present invention;

[0035] Figure 7 This is a schematic diagram of the structural arrangement of the middle and lower base of the bracket disclosed in the embodiment of the present invention;

[0036] Figure 8 This is a schematic diagram of the structure of the active polarizing rod disclosed in an embodiment of the present invention.

[0037] The attached figures are labeled as follows:

[0038] 100. Mixing and heating device for nucleic acid extraction;

[0039] 1. Bracket; 11. Upper support; 12. Middle support; 13. Lower support; 14. Upper column; 15. Lower column; 16. Outer shell;

[0040] 2. Sample mixing module; 21. Positioning frame; 22. Positioning slot; 23. Fixing component; 24. Mixing drive motor; 25. Active polarizing rod; 251. First section rod; 252. Second section rod; 253. Rod connector; 26. Driven polarizing rod;

[0041] 3. Sample heating module; 31. Heating block; 32. Heating rod; 33. Receiving groove; 34. Lifting drive motor; 35. Lead screw; 36. Lead screw nut; 37. Guide rod; 38. Heat insulation block; 39. Bearing seat;

[0042] 4. Consumables;

[0043] 5. Driver circuit board;

[0044] 6. Heating rod connecting plate;

[0045] 7. Heating and temperature control circuit board. Detailed Implementation

[0046] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0047] One of the objectives of this invention is to provide a mixing and heating device for nucleic acid extraction, which solves the problem that existing extraction equipment has an independently set mixing unit structure and a heating unit structure, which not only occupies a lot of space, but also requires frequent transfer of the extract between the mixing unit structure and the heating unit structure, resulting in low experimental efficiency and easy cross-contamination.

[0048] Another object of the present invention is to provide a nucleic acid extraction system having the above-mentioned mixing and heating device for nucleic acid extraction.

[0049] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0050] Example 1

[0051] like Figure 1 As shown, this embodiment provides a mixing and heating device 100 for nucleic acid extraction, mainly including a support 1, a sample mixing module 2, and a sample heating module 3. The sample mixing module 2 is disposed on the support 1 and includes a positioning frame 21 and a mixing drive mechanism connected to the positioning frame 21. The positioning frame 21 is used to place consumables 4 that can hold the extraction sample. The mixing drive mechanism is used to drive the positioning frame 21 to generate a shaking motion, so that the extraction sample in the consumable 4 is shaken and mixed. The sample heating module 3 is disposed on the support 1 and is used to heat the extraction sample in the consumable 4. By placing the sample mixing module 2 and the sample heating module 3 on the same support 1, the sample mixing module 2 and the sample heating module 3 are integrated into a single unit. This not only results in a compact structure, but also allows the consumable 4 to be disposed on the positioning frame 21 of the sample mixing module 2, eliminating the need for transfer between the two processes of shaking and heating. This solves the problem that currently requires frequent transfer of the extract between the mixing unit structure and the heating unit structure, leading to low experimental efficiency and easy cross-contamination.

[0052] In this embodiment, as Figure 1 and Figure 2As shown, consumable 4 is a deep-hole plate. The positioning frame 21 is provided with positioning slots 22 for consumable 4 to pass through and fasteners 23 for fixing the deep-hole plate. Deep-hole plates have many structural forms. Classified by the number of holes, common types include 48-hole plates, 96-hole plates, and 384-hole plates. Classified by hole type, they can be divided into round holes and square holes. Classified by hole bottom shape, the most common types are U-shaped (also known as "round bottom") and V-shaped. Existing common deep-hole plate types can be adapted to this technical solution. Correspondingly, the arrangement of the positioning slots 22 on the positioning frame 21 is also adapted to the different types of deep-hole plates. Taking consumable 4 as an example... Figure 2 Taking the 48-hole round-bottom deep-hole plate as an example, the 48 round-bottom deep holes are arranged in 4 rows of 12 holes each. Correspondingly, the positioning frame 21 is provided with 4 positioning slots 22, which correspond to the 4 rows of round-bottom deep holes on the 48-hole round-bottom deep-hole plate. The 4 rows of deep holes on the 48-hole round-bottom deep-hole plate pass through the 4 positioning slots 22, realizing the installation and positioning of the consumable 4 on the positioning frame 21. Subsequently, the top edge of the 48-hole round-bottom deep-hole plate can be fixed by the fastener 23 to fix the consumable 4 on the positioning frame 21. In order to achieve reliable fixation and facilitate the installation and removal of the consumable 4, the fastener 23 is preferably set as a spring self-resetting buckle in this embodiment; such as Figure 1 and Figure 4 As shown, the spring self-resetting buckle includes an "L"-shaped clip. The corner of the "L"-shaped clip is used to hold the consumable 4. The other end of the "L"-shaped clip is hinged to the bottom edge of the positioning frame 21 via a pivot, and a torsion spring is sleeved on the pivot to keep the "L"-shaped clip perpendicular to the positioning frame 21. When the consumable 4 needs to be installed, it can be installed directly from top to bottom. When the consumable 4 encounters the "L"-shaped clip, the "L"-shaped clip will be pushed outwards. Continue installing the consumable 4 downwards. When the consumable 4 reaches the corner of the "L"-shaped clip, the "L"-shaped clip resets under the action of the torsion spring and becomes perpendicular to the positioning frame 21. At this time, the top plate of the consumable 4 is engaged with the corner of the "L"-shaped clip, thus fixing the consumable 4 in place. When the consumable 4 needs to be removed, the "L"-shaped clip is flipped outwards along its pivot to release the corner of the "L"-shaped clip from the consumable 4, and then the consumable 4 can be removed. The aforementioned spring self-resetting latches are preferably arranged in multiple evenly around the outer periphery of the positioning frame 21, for example, two pairs are symmetrically arranged on both sides of the positioning frame 21. Figure 1 As shown.

[0053] In this embodiment, the positioning frame 21 is located above the support 1. The mixing drive mechanism includes a mixing drive motor 24, an active polarizing rod 25, and a driven polarizing rod 26. The positioning frame 21 is connected to the top of the support 1 through one active polarizing rod 25 and at least one driven polarizing rod 26. The tops of the active polarizing rod 25 and any one of the driven polarizing rods 26 are rotatably connected to the positioning frame 21 via bearings. The active polarizing rod 25 and any one of the driven polarizing rods 26 are also rotatably connected to the support 1 via bearings. The mixing drive motor 24 is mounted on the support 1 and connected to the bottom end of the active polarizing rod 25. The mixing drive motor 24 is used to drive the active polarizing rod 25 to rotate at high speed. Figure 8 As shown, the active polarizing rod 25 includes a first rod 251 and a second rod 252. The first rod 251 and the second rod 252 are axially connected by a rod connector 253. The first rod 251 and the second rod 252 are arranged in parallel, but their axes do not coincide, that is, the first rod 251 and the second rod 252 are eccentrically arranged. The active polarizing rod 25 has an overall "Z" shaped structure. The rod connector 253 can be an existing connector structure such as a pipe clamp or pipe joint. In the active polarizing rod 25, the first rod 251 is shorter than the second rod 252. The first rod 251 is rotatably connected to the positioning frame 21 through a bearing, while the second rod 252 is rotatably connected to the bracket 1 through a bearing. The end of the second rod 252 is connected to the mixing drive motor 24. Each of the remaining driven polarizing rods 26 has a "Z"-shaped shaft structure, similar to the active polarizing rod 25. That is, the shafts at both ends of the driven polarizing rod 26 are parallel and eccentrically set. One end of the shaft of the driven polarizing rod 26 is rotatably connected to the bracket 1 through a bearing, and the other end is rotatably connected to the positioning frame 21 through a bearing. The only difference between the driven polarizing rod 26 and the active polarizing rod 25 is that the shaft segment of the driven polarizing rod 26 connected to the bracket 1 is shorter than that of the second rod 252. When the mixing drive motor 24 rotates at high speed, it drives the active polarizing rod 25 to rotate around the second section rod 252. In turn, the first section rod 251 drives the positioning frame 21 to rotate. Since the positioning frame 21 is also connected to the support 1 through the driven polarizing rod 26, the rotation of the active polarizing rod 25 will also drive the driven polarizing rods 26 to rotate. Finally, under the action of the active polarizing rod 25 and the driven polarizing rods 26, the positioning frame 21 moves eccentrically relative to the support 1. This causes the extracted sample in the consumable 4 on the positioning frame 21 to also move eccentrically. The extracted sample will generate a vortex phenomenon under the drive of the high-speed eccentric force, thereby achieving the mixing effect.

[0054] The aforementioned positioning frame 21 is connected to the top of the support 1 via an active polarizing rod 25 and at least one driven polarizing rod 26. The active polarizing rod 25 and at least one driven polarizing rod 26 work together to ensure stable support of the positioning frame 21 above the support 1. The positioning frame 21 is generally preferably a rectangular plate structure. To further improve structural stability, it is preferable to use one active polarizing rod 25 and three driven polarizing rods 26 to support the positioning frame 21. Figure 4 As shown, a longer active polarizing rod 25 and three shorter driven polarizing rods 26 are respectively installed at the four corners of the positioning frame 21, which can achieve the effect of shaking the positioning frame 21 while providing stable support.

[0055] In this embodiment, the sample heating module 3 is located below the positioning frame 21. The sample heating module 3 includes a heating block 31, a heating rod 32 disposed on the heating block 31, and a heating drive mechanism connected to the heating block 31. The heating block 31 is used to heat the extracted sample inside the consumable 4. The heating drive mechanism is used to drive the heating block 31 closer to or away from the bottom of the consumable 4. Generally, when the consumable 4 needs to be heated, the heating drive mechanism drives the heating block 31 closer to the bottom of the consumable 4. When the consumable 4 does not need to be heated, the heating drive mechanism drives the heating block 31 away from the bottom of the consumable 4 to avoid the heating block 31 interfering with the consumable 4. The heating drive mechanism can be of various driving forms; it can move the heating block 31 horizontally or drive the heating block 31 up and down.

[0056] Furthermore, in this embodiment, the heating drive mechanism is preferably located below the heating block 31, and is mainly used to drive the heating block 31 to rise and fall. For example... Figure 6 and Figure 7As shown, to ensure sufficient heating of the sample inside the consumable 4, a receiving groove 33 is provided on the upper part of the heating block 31 to accommodate the bottom of the consumable 4. The number of receiving grooves 33 generally corresponds to the number of rows of deep holes in the consumable 4. For example, if the consumable 4 is the aforementioned 48-hole round-bottom deep-hole plate, the heating block 31 will have 4 receiving grooves 33 to accommodate the 4 rows of deep holes of the 48-hole round-bottom deep-hole plate, that is, each receiving groove 33 corresponds to one row of deep holes. Multiple heating rods 32 are embedded in the lower part of the heating block 31, and the bottom ends of all heating rods 32 are fixed to the same heating rod connecting plate 6. The heating drive mechanism is located below the heating block 31 and is used to drive the heating block 31 to rise and fall relative to the positioning frame 21 so that the receiving grooves 33 cover the bottom of the consumable 4 or move away from the bottom of the consumable 4. When it is necessary to heat the sample inside consumable 4, the heating drive mechanism drives the heating block 31 to rise, so that the receiving groove 33 covers the bottom of consumable 4, thereby heating the sample inside each deep hole; when it is not necessary to heat the sample inside consumable 4, the heating drive mechanism drives the heating block 31 to fall, so that the entire consumable 4 is located on the upper part of the heating block 31. As a lifting drive mechanism, the heating drive mechanism can be selected from existing linear drive components such as screw-slider mechanisms, electric telescopic rods, hydraulic cylinders, or pneumatic cylinders. As a preferred embodiment, a screw-slider mechanism is selected for the heating drive mechanism, such as... Figure 6 and Figure 7 As shown, the heating drive mechanism includes a lifting drive motor 34, a lead screw 35, a lead screw nut 36, and a guide rod 37. The lifting drive motor 34 is located at the bottom of the bracket 1. The bottom end of the lead screw 35 is connected to the lifting drive motor 34. The lead screw nut 36 is threadedly connected to the lead screw 35, and the top of the lead screw nut 36 is connected to the bottom of the heating block 31. The bottom of the heating block 31 is also provided with a clearance hole for the lead screw 35 to pass through. The diameter of the clearance hole is larger than the diameter of the lead screw 35 to ensure that after the lead screw 35 is inserted into the clearance hole, the clearance hole will not obstruct the continued rotation of the lead screw, and the depth of the clearance hole is sufficient to allow the heating block 31 to detach from the bottom of the consumable 4. In addition, the clearance hole is arranged to avoid the receiving groove 33 and the heating rod 32, and will not affect the normal heating function and deep hole receiving function of the heating block 31. The guide rod 37 is arranged parallel to the lead screw 35, the top end of the guide rod 37 is connected to the bottom of the heating block 31, and the bottom end of the guide rod 37 is slidably engaged with the bracket 1. The lifting drive motor 34 drives the lead screw 35 to rotate in the forward or reverse direction, which in turn drives the heating block 31 to slide up and down along the guide rod 37.

[0057] In this embodiment, the depth of the receiving groove 33 on the heating block 31 is preferably such that it can completely cover the structure below the top plate of the consumable, so as to ensure that the sample inside the consumable is completely covered by the heating block 31 during heating. In this embodiment, a heat insulation block 38 is also provided at the bottom of the heating block 31, and the top end of the guide rod 37 is connected to the heat insulation block 38; the heat insulation block 38 is mainly used to block the heat of the heating block 31 from being transferred to other components, so as to ensure the heating effect and heating efficiency of the sample.

[0058] In this embodiment, the support 1 is preferably a three-layer structure frame, such as... Figure 5 As shown, it includes an upper support 11, a middle support 12, and a lower support 13 arranged sequentially from top to bottom. Any adjacent supports are connected by a column; specifically, the upper support 11 and the middle support 12 are connected by an upper column 14, and the middle support 12 and the lower support 13 are connected by a lower column 15. The upper support 11, middle support 12, and lower support 13 are all rectangular plate structures, with the upper support 11 and the middle support 12 supported by columns located at their four corners. The aforementioned positioning frame 21 is positioned above the upper support 11, and the heating block 31 is positioned between the upper support 11 and the middle support 12. To ensure that the heating block 31 can make normal contact with the consumable 4, a hollow structure can be provided at the center of the upper support 11 to expose the bottom of the consumable 4 placed on the heating block 31. The positioning frame 21 is supported and connected to the edge of the upper support 11 by the active polarizing rod 25 and the driven polarizing rod 26. Correspondingly, the hollow structure is large enough that its sidewalls will not obstruct the vibration of the consumable 4. At the same time, in order to improve space utilization, the mixing drive motor 24 and the lifting drive motor 34 in this embodiment can be set on the middle support 12 or on the lower support 13. When the mixing drive motor 24 is set on the lower support 13, the active polarizing rod 25 passes through the upper support 11 and the middle support 12 and is connected to the output end of the mixing drive motor 24 through a coupling. Similarly, the bottom end of the lead screw 35 passes through the middle support 12 and is connected to the output end of the lifting drive motor 34. The aforementioned middle support 12 also serves to slide with the guide rod 37. A bearing seat 39 is mounted on the middle support 12, and the guide rod 37 can be slidably connected to the bearing seat 39 via a linear bearing. To improve the lifting stability of the heating block 31, it is preferable to set two guide rods 37 on each side of the lead screw 35, for a total of four guide rods 37. Each guide rod 37 has the same fit with the middle support 12, which will not be described in detail here. The heating block 31 is guided by two guide rods 37, improving the stability of its lifting process. In this embodiment, the bracket 1 is configured with the aforementioned layered structure, realizing the layered arrangement of the sample mixing module and the sample heating module on the bracket 1. This not only results in a reasonable layout but also further enhances the structural compactness of the integrated sample heating module and sample mixing module, improving space utilization.

[0059] In this embodiment, three sets of sample heating modules can be arranged side by side on the support 1, and the heating blocks 31 in the three sets of sample heating modules can heat the consumable 4 together.

[0060] In practice, the support 1 can take various structural forms. The multi-layered structural arrangement described above is only one manifestation of it. The specific structure of the support 1 can be adjusted and improved according to actual needs. In addition, in order to ensure the overall dust protection of the device, especially the motors inside the device, an outer shell 16 can be installed around the frame of the support 1, which is both aesthetically pleasing and dustproof, achieving multiple benefits.

[0061] In this embodiment, to further improve the automation level of the mixing and heating device 100 for nucleic acid extraction, a control module can be configured. The control module is electrically connected to the heating rod 32, the lifting drive motor 34, and the mixing drive motor 24. For example, a drive circuit board 5 and a heating temperature control circuit board 7 can be set in the control module. The drive circuit board 5 can control the start, stop, speed, and direction of the lifting drive motor 34 and the mixing drive motor 24. The heating temperature control circuit board 7 can work with temperature sensors and other detection modules to control the heating temperature of each heating rod 32 to ensure that the sample in the consumable 4 is heated within a reasonable temperature range, avoiding overheating or underheating of the sample. The heating rod 32 is a conventional heating structure, such as a resistance wire heating rod. The heating rods 32 are preferably evenly distributed in an array within the heating block 31. The heating rods 32 on the same heating block 31 are welded to the same heating rod connecting plate 6, which is preferably fixed to the bottom of the heating block 31.

[0062] The following is a detailed description of how to use the above-mentioned mixing and heating device 100 for nucleic acid extraction in this embodiment.

[0063] The consumable 4 containing the nucleic acid extraction sample is installed on the positioning frame 21 and then fixed in place by the fastener 23. The heating block 31 is then raised by the lifting drive motor 34 so that the receiving groove 33 on the heating block 31 covers the bottom of the deep hole in the consumable 4. The heating rod 32 is then activated to heat the sample inside the consumable 4. After heating, the heating block 31 is lowered by the lifting drive motor 34 to remove it from the bottom of the consumable 4. The mixing drive motor 24 is then activated to shake the positioning frame 21, ensuring the sample inside the consumable 4 is mixed evenly under shaking. In practice, the sample can also be mixed evenly first before heating it with the heating block 31; the specific operating procedure can be adjusted according to actual needs.

[0064] Therefore, the nucleic acid extraction mixing and heating device 100 proposed in this technical solution is an integrated mixing and heating device. By placing the sample mixing module and the sample heating module on the same support, it achieves an integrated arrangement of the sample mixing module and the sample heating module. This not only results in a compact structure, but also allows consumables to be placed on the positioning frame of the sample mixing module, completing both the shaking and heating processes without the need for transfer. This solves the problem of frequent transfer of extracts between the mixing unit structure and the heating unit structure, which leads to low experimental efficiency and cross-contamination. Furthermore, the support is designed with a layered structure, allowing for a layered arrangement of the sample mixing module and the sample heating module on the support. This not only provides a reasonable layout but also further enhances the compactness of the integrated sample heating module and sample mixing module, improving space utilization.

[0065] The nucleic acid extraction mixing and heating device 100 proposed in this technical solution can realize fully automated operation of sample heating and mixing by configuring a corresponding control module. The operation process is simple and efficient, avoiding the problem of low experimental efficiency caused by the semi-automated operation of existing extraction equipment, which still requires manual operation.

[0066] Example 2

[0067] This embodiment proposes a nucleic acid extraction system, including a nucleic acid extraction device and a mixing and heating device 100 for nucleic acid extraction as disclosed in Embodiment 1, and may also include consumables 4. The aforementioned mixing and heating device 100 for nucleic acid extraction is only one part of the entire molecular diagnostic nucleic acid extraction experiment, mainly used for mixing and heating the extracted nucleic acid, realizing the integration of sample mixing and heating.

[0068] It should be noted that, for those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention, and no reference numerals in the claims should be construed as limiting the scope of the claims.

[0069] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A mixing and heating device for nucleic acid extraction, characterized by comprising: include: The support frame includes an upper support, a middle support, and a lower support arranged sequentially from top to bottom, and any adjacent supports are connected by a support column. A sample mixing module is mounted on the support. The sample mixing module includes a positioning frame and a mixing drive mechanism connected to the positioning frame. The positioning frame holds consumables for holding the extracted sample. The mixing drive mechanism drives the positioning frame to move, causing the extracted sample within the consumables to vibrate and mix. The consumables are deep-hole plates. The positioning frame has positioning slots for the consumables to pass through and fixing members for securing the deep-hole plate. The positioning frame is positioned above the upper support. The mixing drive mechanism... The structure includes a mixing drive motor, an active polarizing rod, and a driven polarizing rod. The positioning frame is connected to the top of the support through one active polarizing rod and at least one driven polarizing rod. The top of the active polarizing rod and any one of the driven polarizing rods are rotatably connected to the positioning frame through bearings. The active polarizing rod and any one of the driven polarizing rods are also rotatably connected to the support through bearings. The mixing drive motor is mounted on the support and connected to the bottom end of the active polarizing rod. The mixing drive motor is used to drive the positioning frame to vibrate relative to the support. A sample heating module is disposed on the support and used to heat the extracted sample inside the consumable. The sample heating module is located below the positioning frame and includes a heating block, heating rods disposed on the heating block, and a heating drive mechanism connected to the heating block. The heating block is disposed between the upper support and the middle support. The heating block is used to heat the extracted sample inside the consumable. The heating drive mechanism is used to drive the heating block closer to or away from the bottom of the consumable. The upper part of the heating block is provided with a receiving groove that can be embedded in the bottom of the consumable. Multiple heating rods are embedded in the lower part of the heating block. The heating drive mechanism is disposed below the heating block and is used to drive the heating block to rise and fall relative to the positioning frame so that the receiving groove covers or moves away from the bottom of the consumable. The bottom of the heating block is also provided with a heat insulation block, and the top end of the guide rod is connected to the heat insulation block. The control module is electrically connected to the heating block, the heating drive mechanism, and the mixing drive mechanism. The control module is equipped with a drive circuit board and a heating temperature control circuit board. The drive circuit board controls the start, stop, speed, and direction of the heating drive mechanism and the mixing drive mechanism. The heating temperature control circuit, in conjunction with a temperature sensor, ensures that the sample in the consumable is heated within a reasonable temperature range.

2. The mixing and heating apparatus for nucleic acid extraction according to claim 1, characterized in that, The fastener is a spring-loaded self-resetting buckle.

3. The mixing and heating device for nucleic acid extraction according to claim 1, characterized in that, The heating drive mechanism includes: A lifting drive motor is located at the bottom of the bracket; A lead screw, the bottom end of which is connected to the lifting drive motor; A lead screw nut, wherein the lead screw nut is threadedly connected to the lead screw, and the top of the lead screw nut is connected to the heating block, and the bottom of the heating block is provided with a clearance hole for the lead screw to pass through; A guide rod is provided parallel to the lead screw. The top end of the guide rod is connected to the heating block, and the bottom end of the guide rod is slidably engaged with the bracket.

4. A nucleic acid extraction system, characterized in that, It includes a nucleic acid extraction device and a mixing and heating device for nucleic acid extraction as described in any one of claims 1 to 3.

Images