Sequencing blockers and their applications
By using linker blocking agents with specific modifications to block nucleotide sequences, the problem of non-target DNA sequence interference in NGS sequencing is solved, which significantly improves the capture efficiency and data utilization, and is suitable for panels of various sizes.
Patent Information
- Application Number
- CN202211545593.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-12-04
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2042-12-04
AI Technical Summary
In NGS sequencing, the non-target DNA sequence introduced during library construction interferes with the binding of probes and target regions, resulting in a reduction in capture efficiency. The existing domestic dual index linker blocking agents have few types of choices, low blocking effect, and high synthesis recovery rate and high cost.
A library member sequence linker blocking agent for NGS sequencing is provided, which comprises a specific blocking nucleotide sequence with modifications for improving Tm values, such as LNA modification and 3' terminal group modification, which can effectively block the linker of the library member sequence and improve capture efficiency.
It significantly reduces non-specific capture interference, enhances the capture effect, and improves data utilization. The blocking agent is suitable for panels of various sizes, and the capture rate is better than the existing blocking agents on the market.