A novel bio-enzyme detection method for dihydromyricetin

The novel bioenzyme detection method for dihydromyricetin simplifies the detection process for small and medium-sized enterprises (SMEs), solving the problem of high cost and complex operation of high-performance liquid chromatography (HPLC) analysis, which is difficult for SMEs to afford. It achieves low-cost, rapid, sensitive and accurate detection results, promoting the advancement of food testing technology and market monitoring.

CN116539548BActive Publication Date: 2026-07-24TONGREN UNIV
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Patent Information

Application Number
CN202310308788.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-03-27
Publication Date
2026-07-24
Estimated Expiration
2043-03-27

AI Technical Summary

Technical Problem

Small and medium-sized enterprises cannot afford the high cost and complex operation of high-performance liquid chromatography (HPLC) analysis technology, resulting in high cost and long time for dihydromyricetin detection, making it impossible to distinguish crude product specifications in a timely manner and increasing market risks.

Method used

A novel bioenzymatic detection method for dihydromyricetin was adopted, which directly and quantitatively analyzes dihydromyricetin in plant-derived samples by reacting dihydromyricetin standard with purine nucleoside phosphorylase solution, simplifying the operation and reducing equipment costs and detection time.

Benefits of technology

It enables low-cost, rapid, sensitive and accurate detection of dihydromyricetin, providing reliable product quality assurance, reducing market risks, and promoting the advancement of food testing technology and market monitoring.

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Abstract

The application discloses a novel dihydromyricetin biological enzyme detection method, relates to the technical field of sample tea quantitative analysis and detection, and has the technical solution as follows: S1, a gradient concentration dihydromyricetin solution is prepared; S2, a purine nucleoside phosphorylase reaction solution is obtained; S3, the absorbance value of a specified wavelength is measured by ultraviolet spectrometry, a standard working curve is drawn, and a curve equation is obtained; S4, a sample tea with a specified mass is weighed and placed in pure water, a to-be-measured solution is obtained through water bath boiling, the to-be-measured solution is measured and diluted to a certain concentration, and a diluted to-be-measured sample solution is obtained; S5, the absorbance value of a specified wavelength is measured by ultraviolet spectrometry, data are recorded, the working curve equation is substituted, and dihydromyricetin content information in the sample tea is obtained. The method has the characteristics of high sensitivity, high precision, short detection time and simple operation, and has the effect of accurate quantitative analysis without separation of target components in a complex environment.
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