A viscosity detection method and its application
The prepared AH fluorescent probe solves the problem of the inability to detect intracellular viscosity in existing technologies, and realizes high-precision, environmentally unaffected mitochondrial viscosity detection with good biocompatibility and targeting.
Patent Information
- Application Number
- CN202310547384.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-12
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2043-05-12
AI Technical Summary
Existing viscosity detection methods cannot measure intracellular viscosity, traditional methods are not applicable, and existing fluorescent probes have short emission wavelengths, complex molecular structures, small Stokes shifts, and severe interference from biological autofluorescence, making them unable to effectively detect changes in mitochondrial viscosity within cells.
Using the fluorescent probe shown in Formula 1, a viscosity detection method with good biocompatibility and unaffected by environmental pH was developed by detecting fluorescence intensity. The preparation method includes reacting 4-methylpyridine with iodomethane and then binding it with 4-dimethylaminocinnamaldehyde to prepare the AH fluorescent probe for targeted mitochondrial viscosity detection.
It achieves high-precision detection of intracellular mitochondrial viscosity, has good biocompatibility and anti-interference properties, can accurately identify viscosity changes in complex environments, and can target mitochondria for detection.