A lysozyme toothpaste for fresh breath and its preparation method

Abstract

This invention belongs to the field of toothpaste technology, specifically relating to a lysozyme toothpaste for fresh breath and its preparation method. The preparation method includes the following steps: lysozyme, lysostaphin, and nisin are mixed evenly; xanthan gum is added and stirred evenly; cellulose gum is added and stirred evenly; microcrystalline cellulose is added and stirred evenly to obtain bio-encapsulated particles; friction-type hydrated silica, thickening hydrated silica, foaming agent, stabilizer, and water are mixed and stirred evenly to obtain a friction emulsion; bio-encapsulated particles, friction emulsion, peppermint flavoring, humectant, sweetener, preservative, sodium phytate, dipotassium glycyrrhizate, and allantoin are mixed and stirred evenly to obtain the lysozyme toothpaste for fresh breath. The toothpaste prepared in this application has good storage stability and can quickly and fully release its active ingredients during brushing, with excellent fragrance release. The lysozyme in the toothpaste has good thermal stability and excellent antibacterial and antimicrobial effects.

Description

Technical Field

[0001] This invention belongs to the field of toothpaste technology, specifically relating to a lysozyme toothpaste for fresh breath and its preparation method. Background Technology

[0002] Toothpaste is a commonly used oral hygiene product. Its basic functions are to clean teeth, freshen breath, and maintain oral hygiene. Oral problems are mainly caused by bacteria. Using effective toothpaste, mouthwash, and oral disease treatments can significantly reduce the number of pathogenic bacteria in the mouth and improve or even eliminate oral diseases. Oral halitosis is caused by oral problems such as excessive accumulation of plaque, tartar, and calculus, as well as gingivitis, periodontitis, oral mucosal diseases, and tooth decay.

[0003] Lysozyme is a natural antibacterial protein widely found in plant sap, animal secretions, tears, saliva, breast milk, eggs, and some bacteria. Lysozyme not only possesses muramase activity, hydrolyzing the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, but also exhibits antimicrobial peptide activity. This is related to the structure, charge distribution, and surface hydrophobicity of lysozyme, resulting in good safety. For example, patent CN112336678A discloses a breath-freshening toothpaste comprising the following components by weight: 1-5 parts lysozyme, 1-5 parts borneol, 2-3 parts osmanthus, 2-5 parts plant extracts, and 8-12 parts propolis. This breath-freshening toothpaste uses lysozyme, borneol, and osmanthus as its main active ingredients. The synergistic effect of these substances effectively combats Helicobacter pylori, enhances antibacterial and antimicrobial properties, removes odors, and freshens breath.

[0004] However, during the research process, the applicant found that most toothpastes are weakly alkaline, and lysozyme has poor thermal stability under alkaline conditions, which reduces the antibacterial and antimicrobial effects of lysozyme in toothpaste. Summary of the Invention

[0005] To address the drawback of poor thermal stability of lysozyme in toothpaste, this application provides a lysozyme toothpaste for fresh breath and a method for preparing the same.

[0006] In a first aspect, this application provides a method for preparing a lysozyme toothpaste that freshens breath, achieved using the following technical solution:

[0007] A method for preparing a lysozyme toothpaste for freshening breath includes the following steps:

[0008] Lysozyme, lysostaphylococcal enzyme, and nisin are mixed evenly, xanthan gum is added, and the mixture is stirred evenly. Then cellulose gum is added and stirred evenly, followed by microcrystalline cellulose and stirred evenly to obtain bio-enzyme-encapsulated particles. The mass ratio of lysozyme, lysostaphylococcal enzyme, nisin, xanthan gum, cellulose gum, and microcrystalline cellulose is (0.02-0.05):(0.01-0.02):(0.02-0.05):(0.8-1):(0.5-1):(2-3).

[0009] Friction-type hydrated silica, thickening hydrated silica, foaming agent, stabilizer and water are mixed and stirred evenly to obtain a friction emulsion; the mass ratio of friction-type hydrated silica, thickening hydrated silica, foaming agent, stabilizer and water is (10-12):(8-12):(6-10):(0.1-0.2):(8.56-28.14);

[0010] A lysozyme toothpaste for fresh breath is prepared by mixing bio-enzyme-encapsulated particles, a friction emulsion, a peppermint flavoring, a humectant, a sweetener, a preservative, sodium phytate, and dipotassium glycyrrhizate, and stirring until homogeneous. The mass ratio of the bio-enzyme-encapsulated particles, friction emulsion, peppermint flavoring, humectant, sweetener, preservative, sodium phytate, dipotassium glycyrrhizate, and allantoin is (3.35-5.12):(42.76-52.24):(0.8-1.2):(42-48):(0.2-0.3):(0.3-0.4):(1-2):(0.1-0.2):(0.01-0.02).

[0011] By adopting the above technical solution, microcrystalline cellulose and active ingredients work together to keep the toothpaste paste smooth and have good storage stability. At the same time, the active ingredients in the paste are released quickly and fully when brushing, resulting in better diffusion and superior aroma release.

[0012] Compared to abrasives such as calcium carbonate, aluminum hydroxide, and silica, hydrated silica has a lower abrasive value, does not damage gums, produces a finer paste, and spreads better, allowing it to be made into transparent or semi-transparent toothpaste. This application includes abrasive-type and thickening-type hydrated silica; the use of microcrystalline cellulose can reduce the amount of thickening-type hydrated silica required.

[0013] This application uses xanthan gum, cellulose gum, and microcrystalline cellulose to sequentially encapsulate a mixture of biological enzymes. Xanthan gum is a widely used microbial extracellular polysaccharide produced through fermentation engineering. Microcrystalline cellulose is a crystalline powder composed of porous microparticles with a large specific surface area, exhibiting good adhesion and stability. As the third layer of encapsulation material for the biological enzyme mixture, microcrystalline cellulose works in conjunction with cellulose gum to significantly improve the stability and antibacterial and antimicrobial effects of the biological enzyme mixture. It can also improve the thermal stability of lysozyme in toothpaste, thereby enhancing the antibacterial effect of toothpaste after being stored at 45°C for 6 months.

[0014] This application uses a mixture of lysostaphin, lysozyme and nisin, which are bioenzymes. The interaction between lysostaphin and lysozyme, as well as the interaction between nisin and lysozyme, can improve the antibacterial effect of toothpaste and alleviate the defect of poor thermal stability of lysozyme in toothpaste, thereby improving the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0015] Preferably, the mass ratio of lysozyme, lysostaphylococcal enzyme, nisin, xanthan gum, cellulose gum and microcrystalline cellulose is 0.04:0.02:0.05:1:0.8:3.

[0016] By adopting the above technical solution, the lysozyme in the toothpaste has better thermal stability, and the antibacterial and antimicrobial effects of the toothpaste are better, especially the antibacterial effect after being placed at 45℃ for 6 months.

[0017] Preferably, the foaming agent is a mixture of sodium lauroyl sarcosinate and cocamidopropyl betaine in a mass ratio of (4-6):(2-4).

[0018] By adopting the above technical solution, sodium lauroyl sarcosinate has high safety for the environment and organisms, and its effects are mild. It can effectively inhibit the growth of bacteria in human saliva, and it can firmly adhere to dental plaque and maintain its activity for a long time. The foaming agent, which is a combination of cocamidopropyl betaine and sodium lauroyl sarcosinate, can produce rich foam when brushing teeth, reduce the adhesion of plaque and food debris to the tooth surface, and disperse them into fine particles to form an emulsion system. When rinsing the mouth, it is rinsed away with water, thereby achieving the purpose of removing plaque and cleaning the oral cavity.

[0019] Preferably, the stabilizer is tetrasodium pyrophosphate.

[0020] By adopting the above technical solution, tetrasodium pyrophosphate possesses the general properties of ordinary polyphosphates, including emulsifying and dispersing properties, which can improve the antioxidant properties of toothpaste paste and thus improve the storage stability of toothpaste.

[0021] Preferably, the mass ratio of the microcrystalline cellulose, peppermint flavor, sodium phytate and dipotassium glycyrrhizate is 3:1.2:1.5:0.2.

[0022] By adopting the above technical solution, peppermint flavor, sodium phytate, and dipotassium glycyrrhizate are all active ingredients. Among them, dipotassium glycyrrhizate has multiple effects such as antibacterial, anti-allergic, and deodorizing, and acts as a sweetener, preservative, and flavor enhancer. Sodium phytate is an important pure natural green additive with strong antioxidant properties. Sodium phytate and dipotassium glycyrrhizate have no effect on lysozyme activity. Microcrystalline cellulose works together with peppermint flavor, sodium phytate, and dipotassium glycyrrhizate to keep the toothpaste paste smooth and have good storage stability. At the same time, the active ingredients in the paste are released quickly and fully when brushing, the toothpaste has better diffusion, and the aroma release effect is better.

[0023] Preferably, the moisturizer is a mixture of sorbitol and polyethylene glycol in a mass ratio of (40-45):(2-3).

[0024] By adopting the above technical solution, sorbitol has good moisturizing properties and can also maintain the aroma of toothpaste. The humectant compounded with sorbitol and polyethylene glycol can lock in moisture and improve the storage stability of toothpaste.

[0025] Preferably, the polyethylene glycol is polyethylene glycol-8.

[0026] By employing the above-mentioned technical solution, polyethylene glycol-8 is relatively mild, possessing both moisturizing and abrasive properties, thus increasing the toothpaste's abrasiveness and achieving the purpose of removing plaque and cleaning the oral cavity. Furthermore, polyethylene glycol-8 can also work synergistically with dipotassium glycyrrhizate, sodium lauroyl sarcosinate, and cocamidopropyl betaine to enhance the antibacterial effect of the toothpaste.

[0027] Secondly, this application provides a lysozyme toothpaste for freshening breath, achieved using the following technical solution:

[0028] A lysozyme toothpaste for freshening breath is prepared by the above-described method.

[0029] Preferably, the composition and weight percentage of the components of the breath-freshening lysozyme toothpaste are as follows:

[0030] Lysozyme 0.02-0.05%

[0031] Lysostaphin 0.01-0.02%

[0032] 0.02-0.05% of nisin

[0033] Xanthan gum 0.8-1%

[0034] Cellulose gum 0.5-1%

[0035] Microcrystalline cellulose 2-3%

[0036] Friction-type hydrated silica 10-12%

[0037] Thickening hydrated silica 8-12%

[0038] 6-10% foaming agent

[0039] Stabilizer 0.1-0.2%

[0040] Peppermint flavoring 0.8-1.2%

[0041] Moisturizer 42-48%

[0042] Sweetener 0.2-0.3%

[0043] Preservative 0.3-0.4%

[0044] Sodium phytate 1-2%

[0045] Dipotassium glycyrrhizate 0.1-0.2%

[0046] Allantoin 0.01-0.02%

[0047] Water balance;

[0048] The sum of the weight percentages of all components is 100%.

[0049] By adopting the above technical solution, the toothpaste contains a variety of functional ingredients, which can be quickly and fully released when brushing teeth. The microcrystalline cellulose works together with the functional ingredients to improve the release effect of the minty aroma of the toothpaste, while also having good storage stability.

[0050] In summary, this application has the following beneficial effects:

[0051] 1. This application uses microcrystalline cellulose together with peppermint flavoring, sodium phytate and dipotassium glycyrrhizate to keep the toothpaste paste smooth and have good storage stability. At the same time, the active ingredients in the paste are released quickly and fully when brushing, and the toothpaste has better diffusion and better fragrance release.

[0052] 2. Compared to abrasives such as calcium carbonate, aluminum hydroxide, and silica, the hydrated silica in this application has a lower abrasive value, does not damage the gums, produces a finer paste, and has better spreadability, allowing it to be made into transparent or semi-transparent toothpaste. The hydrated silica in this application includes abrasive-type and thickening-type hydrated silica; the use of microcrystalline cellulose can reduce the amount of thickening-type hydrated silica required.

[0053] 3. In this application, xanthan gum, cellulose gum, and microcrystalline cellulose are used to sequentially encapsulate the bio-enzyme mixture. Microcrystalline cellulose is a crystalline powder composed of porous microparticles with a large specific surface area, exhibiting good adhesion and stability. As the third layer of encapsulation material for the bio-enzyme mixture, microcrystalline cellulose works together with cellulose gum to significantly improve the stability and antibacterial and antimicrobial effects of the bio-enzyme mixture. It can also improve the thermal stability of lysozyme in toothpaste, thereby enhancing the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0054] 4. This application uses a mixture of lysostaphin, lysozyme and nisin, which are biological enzymes. The interaction between lysostaphin and lysozyme, as well as the interaction between nisin and lysozyme, can improve the antibacterial effect of toothpaste and alleviate the defect of poor thermal stability of lysozyme in toothpaste, thereby improving the antibacterial effect of toothpaste after being placed at 45°C for 6 months. Detailed Implementation

[0055] The present application will be further described in detail below with reference to the embodiments.

[0056] Example

[0057] Examples 1-10 provide a lysozyme toothpaste for freshening breath. The following description uses Example 1 as an example.

[0058] The lysozyme toothpaste for freshening breath provided in Example 1 is prepared using the following steps:

[0059] S1. Mix 0.02g egg white lysozyme, 0.01g lysostaphin and 0.02g nisin evenly, add 0.8g xanthan gum, stir evenly, then add 0.5g cellulose gum, stir evenly, then add 2g microcrystalline cellulose, stir evenly to obtain bio-enzyme-encapsulated particles.

[0060] S2. Mix 10g of friction-type hydrated silica, 8g of thickening-type hydrated silica, 4g of sodium lauroyl sarcosinate, 2g of cocamidopropyl betaine, 0.1g of tetrasodium pyrophosphate and 28.14g of water, and stir well to obtain a friction emulsion.

[0061] S3. Mix the bio-enzyme-encapsulated particles prepared in step S1, the friction emulsion prepared in step S2, 0.8g of peppermint flavoring, 40g of sorbitol, 2g of polyethylene glycol-8, 0.3g of benzyl alcohol, 1g of sodium phytate, 0.1g of dipotassium glycyrrhizate and 0.01g of allantoin, and stir well to obtain a lysozyme toothpaste for fresh breath.

[0062] Among them, egg white lysozyme with an enzyme activity of 2000 U / mg was purchased from Nanning Pangbo Biotechnology Co., Ltd.

[0063] Lysostaphin, enzyme activity 1200U / mg, purchased from Shanghai Shifeng Biotechnology Co., Ltd.

[0064] The lactobacillus peptide was purchased from Hubei Xinmingtai Chemical Co., Ltd.

[0065] Examples 2-10 differ from Example 1 only in that the mass of each component is different, as shown in Table 1.

[0066] Table 1. Mass / g of each component in Examples 1-10

[0067]

[0068]

[0069] Comparative Example

[0070] Comparative Example 1 differs from Example 1 only in that the lysostaphylococcal enzyme is replaced with egg white lysozyme.

[0071] Comparative Example 2 differs from Example 1 only in that the egg white lysozyme is replaced with lysostaphin enzyme.

[0072] Comparative Example 3 differs from Example 1 only in that nisin is replaced with lysostaphin.

[0073] Comparative Example 4 differs from Example 1 only in that lysostaphin is replaced with nisin.

[0074] Comparative Example 5 differs from Example 1 only in the preparation steps of step S1, which are as follows:

[0075] S1. Mix 0.02g egg white lysozyme, 0.01g lysostaphin and 0.02g nisin evenly, add 1.3g xanthan gum, stir evenly, then add 2g microcrystalline cellulose and stir evenly to obtain bio-enzyme-encapsulated particles.

[0076] Comparative Example 6 differs from Example 1 only in the preparation steps of step S1, which are as follows:

[0077] S1. Mix 0.02g of egg white lysozyme, 0.01g of lysostaphin and 0.02g of nisin evenly, add 1.3g of cellulose gum, stir evenly, then add 2g of microcrystalline cellulose and stir evenly to obtain bio-enzyme-encapsulated particles.

[0078] Comparative Example 7 differs from Example 1 only in the preparation steps of step S1, specifically:

[0079] S1. Mix 0.02g of egg white lysozyme, 0.01g of lysostaphin and 0.02g of nisin evenly, add 0.8g of xanthan gum, stir evenly, then add 2.5g of cellulose gum and stir evenly to obtain bio-enzyme-encapsulated particles.

[0080] Comparative Example 8 differs from Example 1 only in the preparation steps of step S1, which are as follows:

[0081] S1. Mix 0.02g of egg white lysozyme, 0.01g of lysostaphin and 0.02g of nisin evenly, add 0.8g of xanthan gum, stir evenly, then add 2.5g of microcrystalline cellulose and stir evenly to obtain bio-enzyme-encapsulated particles.

[0082] Comparative Example 9 differs from Example 1 only in the preparation steps of S1 and S2, specifically:

[0083] S1. Mix 0.02g egg white lysozyme, 0.01g lysostaphin and 0.02g nisin evenly, add 0.8g xanthan gum, stir evenly, then add 0.5g cellulose gum and stir evenly to obtain bio-enzyme-encapsulated particles.

[0084] S2. Mix 10g of friction-type hydrated silica, 10g of thickening-type hydrated silica, 4g of sodium lauroyl sarcosinate, 2g of cocamidopropyl betaine, 0.1g of tetrasodium pyrophosphate and 28.15g of water, and stir well to obtain a friction emulsion.

[0085] The lysozyme toothpaste for fresh breath provided in Comparative Example 10 was prepared using the following steps:

[0086] S1. Mix 0.02g of egg white lysozyme, 0.01g of lysostaphin and 0.02g of nisin evenly to obtain a biological enzyme mixture;

[0087] S2. Mix 10g of friction-type hydrated silica, 8g of thickening-type hydrated silica, 4g of sodium lauroyl sarcosinate, 2g of cocamidopropyl betaine, 0.1g of tetrasodium pyrophosphate and 28.15g of water, and stir well to obtain a friction emulsion.

[0088] S3. Mix the bio-enzyme mixture prepared in step S1, the friction emulsion prepared in step S2, 0.8g xanthan gum, 0.5g cellulose gum, 2g microcrystalline cellulose, 0.8g peppermint flavor, 40g sorbitol, 2g polyethylene glycol-8, 0.3g benzyl alcohol, 1g sodium phytate and 0.1g dipotassium glycyrrhizate, and stir well to obtain a lysozyme toothpaste for fresh breath.

[0089] Performance testing

[0090] The antibacterial effect, aroma release effect and storage stability of the lysozyme toothpaste for fresh breath prepared in Examples 1-10 and Comparative Examples 1-10 of this application were tested.

[0091] I. Antibacterial effect

[0092] Preparation of bacterial culture:

[0093] 1. *Actinomyces actinomyces* bacterial culture: *Actinomyces actinomyces* ATCC 29523 was cultured on blood agar (TSA + 5% defibrinated sheep blood) at 37°C and 5% CO2 for 48 h. The bacterial concentration was adjusted to 1 × 10⁻⁶ cells / mL using PBS phosphate buffered saline (pH = 7.2). 9 CFU / mL.

[0094] 2. Streptococcus mutans bacterial culture: Streptococcus mutans CGMCC 12499 was inoculated onto blood agar plates and placed in an anaerobic bag containing 80% N2, 10% H2, and 10% CO2. The culture was then incubated at 37°C for 48 hours, and the bacterial concentration was adjusted to 1×10⁻⁶. 9 CFU / mL.

[0095] 3. Fusobacterium nucleatum bacterial culture: Inoculate Fusobacterium nucleatum ATCC 25586 onto blood agar plates, place them in an anaerobic bag containing 80% N2, 10% H2, and 10% CO2, and incubate at 37°C for 48 hours. Adjust the bacterial concentration to 1×10⁻⁶. 9 CFU / mL.

[0096] Among them, Actinobacillus actinomycetii ATCC 29523 was obtained from the Institute of Microbiology, Guangdong Academy of Sciences, while Streptococcus mutans CGMCC 12499 and Fusobacterium nucleatum ATCC 25586 were obtained from the College of Food Science and Technology, Jiangsu University.

[0097] Preparation of test solution:

[0098] 1. Toothpaste solution A: After repeatedly rubbing 1 mL of the breath-freshening lysozyme toothpaste prepared in Examples 1-10 and Comparative Examples 1-10 with a toothbrush for 5 minutes, add it to 50 mL of water and mix well to obtain toothpaste solution A.

[0099] 2. Toothpaste solution B: After placing the breath-freshening lysozyme toothpaste prepared in Examples 1-10 and Comparative Examples 1-10 in an electric thermostatic drying oven at 45°C for 6 months, take out 1 mL of toothpaste and rub it repeatedly with a toothbrush for 5 minutes, then add it to 50 mL of water and mix well to obtain toothpaste solution B.

[0100] Test of the diameter of the inhibition zone:

[0101] The Oxford cup double-layer plate method was used. The above-mentioned suspensions of Actinobacillus actinomycetii, Streptococcus mutans, and Fusobacterium nucleatum were used respectively. 100 μL of the prepared toothpaste solution A and B were added to the wells of the Oxford cup. After the petri dishes were incubated at 37℃ for 48 h, the diameter of the inhibition zone was measured. The test results are shown in Table 2.

[0102] Table 2 Results of antibacterial effect test

[0103]

[0104]

[0105] The following section details this application based on the test data in Table 2.

[0106] The test data from Example 1 and Comparative Examples 1-2 show that the interaction between lysostaphylococcal enzyme and egg white lysozyme can improve the antibacterial effect and alleviate the defect of poor thermal stability of lysozyme in toothpaste, thereby improving the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0107] The test data from Example 1 and Comparative Examples 3-4 show that the interaction between nisin and egg white lysozyme can improve the antibacterial effect and alleviate the defect of poor thermal stability of lysozyme in toothpaste.

[0108] The test data from Example 1 and Comparative Examples 5-8 show that encapsulating the biological enzyme mixture with xanthan gum, cellulose gum, and microcrystalline cellulose in sequence can alleviate the defect of poor thermal stability of lysozyme in toothpaste and improve the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0109] The test data from Example 1 and Comparative Example 9 show that microcrystalline cellulose is a crystalline powder composed of porous microparticles with a large specific surface area, good adhesion and stability. As a coating material for the third layer of biological enzyme mixture, microcrystalline cellulose can improve the thermal stability of lysozyme in toothpaste, thereby improving the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0110] The test data from Example 1 and Comparative Example 10 show that encapsulating the bio-enzyme mixture with xanthan gum, cellulose gum, and microcrystalline cellulose can significantly improve the stability and antibacterial and antimicrobial effects of the bio-enzyme mixture, and can improve the thermal stability of lysozyme in toothpaste, thereby improving the antibacterial effect of toothpaste after being placed at 45°C for 6 months.

[0111] II. Aroma Release Effect

[0112] One mL of the lysozyme toothpaste for fresh breath prepared in Examples 1-10 and Comparative Examples 1-10 was repeatedly rubbed with a toothbrush for 5 minutes, and the aroma release effect was observed. The aroma release effect was divided into 5 levels, where level 1 indicates poor, level 2 indicates better, level 3 indicates better, level 4 indicates very better, and level 5 indicates excellent. The test results are shown in Table 3.

[0113] III. Storage Stability

[0114] The lysozyme toothpastes for fresh breath prepared in Examples 1-10 and Comparative Examples 1-10 were placed at 25°C for 6 months and then removed to observe the appearance of the samples. The test results are shown in Table 3.

[0115] Table 3. Test results of aroma release effect and storage stability

[0116]

[0117]

[0118] The following section details this application based on the test data in Table 3.

[0119] The test data from Examples 1 and Comparative Examples 7 and 9 show that replacing microcrystalline cellulose with cellulose gum in Comparative Example 7 and replacing microcrystalline cellulose with thickening hydrated silica in Comparative Example 9 reduced the aroma release level of the toothpaste. This is because the addition of microcrystalline cellulose, together with peppermint flavoring, sodium phytate and dipotassium glycyrrhizate, can keep the toothpaste paste smooth and have good storage stability. At the same time, the active ingredients in the paste are released quickly and fully when brushing, resulting in better diffusion and aroma release.

[0120] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A method for preparing a lysozyme toothpaste for freshening breath, characterized in that, Includes the following steps: Egg white lysozyme, lysostaphin, and nisin are mixed evenly, xanthan gum is added, and the mixture is stirred evenly. Then cellulose gum is added and stirred evenly, followed by microcrystalline cellulose and stirred evenly to obtain bio-enzyme-encapsulated particles. The mass ratio of egg white lysozyme, lysostaphin, nisin, xanthan gum, cellulose gum, and microcrystalline cellulose is (0.02-0.05):(0.01-0.02):(0.02-0.05):(0.8-1):(0.5-1):(2-3). Friction-type hydrated silica, thickening hydrated silica, foaming agent, stabilizer and water are mixed and stirred evenly to obtain a friction emulsion; the mass ratio of the friction-type hydrated silica, thickening hydrated silica, foaming agent, stabilizer and water is (10-12):(8-12):(6-10):(0.1-0.2):(8.56-28.14). The bio-enzyme-encapsulated particles, friction emulsion, peppermint flavoring, moisturizer, sweetener, preservative, sodium phytate, and dipotassium glycyrrhizate are mixed and stirred evenly to obtain a lysozyme toothpaste for fresh breath; the mass ratio of the bio-enzyme-encapsulated particles, friction emulsion, peppermint flavoring, moisturizer, sweetener, preservative, sodium phytate, dipotassium glycyrrhizate, and allantoin is (3.35-5.12):(42.76-52.24):(0.8-1.2):(42-48):(0.2-0.3):(0.3-0.4):(1-2):(0.1-0.2):(0.01-0.02).

2. The method for preparing a lysozyme toothpaste for freshening breath according to claim 1, characterized in that, The mass ratio of egg white lysozyme, lysostaphin, nisin, xanthan gum, cellulose gum, and microcrystalline cellulose is 0.04:0.02:0.05:1:0.8:

3.

3. The method for preparing a lysozyme toothpaste for freshening breath according to claim 1, characterized in that, The foaming agent is a mixture of sodium lauroyl sarcosinate and cocamidopropyl betaine in a mass ratio of (4-6):(2-4).

4. The method for preparing a lysozyme toothpaste for freshening breath according to claim 1, characterized in that, The stabilizer is tetrasodium pyrophosphate.

5. The method for preparing a lysozyme toothpaste for freshening breath according to claim 1, characterized in that, The mass ratio of the microcrystalline cellulose, peppermint flavor, sodium phytate, and dipotassium glycyrrhizate is 3:1.2:1.5:0.

2.

6. The method for preparing a lysozyme toothpaste for freshening breath according to claim 1, characterized in that, The moisturizer is a mixture of sorbitol and polyethylene glycol in a mass ratio of (40-45):(2-3).

7. The method for preparing a lysozyme toothpaste for freshening breath according to claim 6, characterized in that, The polyethylene glycol is polyethylene glycol-8.

8. A lysozyme toothpaste for fresh breath prepared according to any one of claims 1-7.

9. A lysozyme toothpaste for freshening breath according to claim 8, characterized in that, The composition and weight percentage of the components of the breath-freshening lysozyme toothpaste are as follows: Egg white lysozyme 0.02-0.05% Lysostaphin 0.01-0.02% 0.02-0.05% of lactobacillus peptides Xanthan gum 0.8-1% Cellulose gum 0.5-1% Microcrystalline cellulose 2-3% Friction-type hydrated silica 10-12% Thickening hydrated silica 8-12% 6-10% foaming agent Stabilizer 0.1-0.2% Peppermint flavoring 0.8-1.2% Moisturizer 42-48% Sweetener 0.2-0.3% Preservative 0.3-0.4% Sodium phytate 1-2% Dipotassium glycyrrhizate 0.1-0.2% Allantoin 0.01-0.02% Water balance; The sum of the weight percentages of all components is 100%.

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