Cosmetic compositions and uses thereof

Abstract

This application provides a cosmetic composition and its use, wherein the cosmetic composition includes a self-assembled composition and an antioxidant component, the self-assembled composition including chondroitin sulfate or its salt, collagen-like substances, and a modifier, and the antioxidant component having a molecular weight <450 and logP≤2.

Description

Technical Field

[0001] This application belongs to the field of cosmetic technology, specifically relating to a cosmetic composition and its use. Background Technology

[0002] When skincare products are applied to the skin, they inevitably come into contact with air. This is especially true for ingredients with strong antioxidant properties, which are themselves excellent reducing agents and thus more easily oxidized. Therefore, effectively preventing the oxidation of antioxidants in the air and allowing them to penetrate the skin more deeply to exert their true effects remains a goal pursued in this field. Summary of the Invention

[0003] To address the above issues, this application, based on previous research on self-assembled compositions, further investigated the effects of these compositions on different antioxidant components, thereby determining a composition capable of effectively protecting antioxidant components with a certain structure, thus completing this invention.

[0004] Specifically, this application relates to the following aspects:

[0005] 1. A cosmetic composition comprising a self-assembled composition and an antioxidant ingredient,

[0006] The self-assembled composition comprises chondroitin sulfate or its salt, collagen-like substances, and a modifier, wherein the modifier has an oil-water partition coefficient (log P) ≤ 4 and a molecular weight of 50-550.

[0007] The oil-water partition coefficient (log P) of the antioxidant component is ≤2.

[0008] Preferably, the molecular weight of the antioxidant component is <450.

[0009] 2. The composition according to item 1, wherein the mass ratio of chondroitin sulfate or its salt to the collagenous substance is 1:(0.5-5), preferably 1:(1-4).

[0010] 3. The composition according to item 1, wherein the mass ratio of chondroitin sulfate or its salt to the antioxidant component is 1:(0.1-30), preferably 1:(0.1-15).

[0011] 4. The composition according to item 1 or 2, wherein the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1 to 1:50.

[0012] 5. The composition according to any one of claims 1-4, wherein the oil-water partition coefficient (log P) of the modifier is -5 to 4.

[0013] Preferably, the modifier is a modifier containing hydroxyl, carboxyl, amino, glycoside, or amide structures.

[0014] More preferably, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose.

[0015] 6. The composition according to any one of items 1-5, wherein the self-assembled composition further comprises a solvent.

[0016] 7. The composition according to any one of items 1-6, wherein the pH value of the composition is greater than 4 and less than 7.

[0017] 8. The composition according to any one of items 1-7, wherein the ionic strength of the composition is <0.26 mol / kg.

[0018] 9. The composition according to any one of items 1-8, wherein the antioxidant component comprises one or more of sodium bisulfite, ascorbic acid or its derivatives, ferulic acid, and ergothioneine.

[0019] 10. Use of the composition of any one of items 1-9 in preventing the antioxidant component from being oxidized.

[0020] This application has the following beneficial effects:

[0021] Based on the self-assembled composition developed in previous research, this application further identifies matching antioxidant components, thereby enabling the self-assembled composition to effectively protect these antioxidant components from oxidation and enhance their penetration into the skin to exert a truly meaningful effect. Attached Figure Description

[0022] Figure 1A This is a scanning electron microscope image that is 100 times larger than that of Example 7.

[0023] Figure 1B This is a scanning electron microscope image at 500x magnification of Example 7. Detailed Implementation

[0024] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.

[0025] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The present application is further described below with reference to specific embodiments, but is not intended to limit the scope of the application.

[0026] To address the problems existing in the prior art, this application provides a cosmetic composition comprising a self-assembled composition and an antioxidant component.

[0027] In this application, antioxidants refer to components that can be oxidized by oxygen, and are not limited to various antioxidants known in the art, but may also include functional components, excipients, etc. that can be oxidized by oxygen.

[0028] The molecular weight of the antioxidant component is <450, for example, it can be 449, 440, 430, 420, 410, 400, 390, 380, 370, 360, 350, 340, 330, 420, 410, 400, 390, 380, 370, 360, 350, 340, 330, 320, 310, 300, 290, 280, 270, 260, 250, 240, 230, 220, 210, 200, 190, 180, 170, 160, 150, 140, 130, 120, 110, 100, 90, 80, and any range between these values.

[0029] The logP (oil-water partition coefficient) of the antioxidant component is ≤2, and can be, for example, 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0, -0.1, -0.2, -0.3, -0.4, -0.5, -0.6, -0.7, -0.8, -0.9, -1, -1 -1, -1.2, -1.3, -1.4, -1.5, -1.6, -1.7, -1.8, -1.9, -2, -2.1, -2.2, -2.3, -2.4, -2.5, -2.6, -2.7, -2.8, -2.9, -3, -3.1, -3.2, -3.3, -3.4, -3.5, -3.6, -3.7, -3.8, -3.9, -4, and any range between these values.

[0030] In some specific embodiments, the molecular weight of the antioxidant component is <400, preferably 100-350.

[0031] In some specific embodiments, the logP of the antioxidant component is -3.5 to 2.

[0032] In some specific embodiments, the antioxidant includes one or more of sodium bisulfite, ascorbic acid or its derivatives, ferulic acid, and ergothioneine.

[0033] In some specific embodiments, the active ingredients and excipients that can be oxidized by oxygen include ellagic acid, glutathione, arbutin, folic acid, catechins, etc.

[0034] In some specific embodiments, the self-assembly composition includes chondroitin sulfate or its salts, collagen-like substances, and modifiers.

[0035] In this application, the collagen-like substances can encompass various types of collagen known in the art, such as animal-derived collagen, which can be extracted from animal skin, tendons, or bone tissue. The collagen-like substances can also be artificially synthesized collagen according to methods known in the art. These collagens can be hydrolyzed collagen or unhydrolyzed collagen.

[0036] In some specific embodiments, the collagen-like substance is hydrolyzed collagen. Hydrolyzed collagen, i.e., the hydrolysis product of collagen, is the product obtained by hydrolyzing collagen. This application does not impose any restrictions on the hydrolysis method; for example, alkaline methods, acid methods, saline-alkali methods, and / or enzymatic methods can be used.

[0037] In some specific embodiments, the hydrolyzed collagen includes gelatin.

[0038] In some specific embodiments, the collagen-like substance includes soluble collagen. Soluble collagen is collagen derived from tissues that can be dissolved, and depending on the soluble solvent used, it can be broadly classified into neutral salt-soluble collagen and acid-soluble collagen.

[0039] In some specific embodiments, the molecular weight of the collagen-like substance is 10kDa-90kDa, for example, it can be 10kDa, 15kDa, 20kDa, 25kDa, 30kDa, 35kDa, 40kDa, 45kDa, 50kDa, 55kDa, 60kDa, 65kDa, 70kDa, 75kDa, 80kDa, 85kDa, 90kDa, and any range between these values.

[0040] In some specific embodiments, the molecular weight of chondroitin sulfate or its salt is 20kDa-50kDa, for example, it can be 20kDa, 21kDa, 22kDa, 23kDa, 24kDa, 25kDa, 26kDa, 27kDa, 28kDa, 29kDa, 30kDa, 31kDa, 32kDa, 33kDa, 34kDa, 35kDa, 36kDa, 37kDa, 38kDa, 39kDa, 40kDa, 41kDa, 42kDa, 43kDa, 44kDa, 45kDa, 46kDa, 47kDa, 48kDa, 49kDa, 50kDa, and any range between these values.

[0041] In some specific embodiments, the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa.

[0042] In some specific embodiments, the oil-water partition coefficient of the modifier is ≤4, for example, it can be 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.5, 0, -0.5, -1, -1.5, -2, -2.5, -3, -3.5, -4, -4.5, -5, -5.5, -6, and any range between these values.

[0043] In some specific embodiments, the oil-water partition coefficient of the modifier is -5 to 4.

[0044] The oil-water partition coefficient (Log P) is the logarithm of the ratio of the equilibrium concentration of a compound in the non-aqueous phase to its equilibrium concentration in the neutral form in the aqueous phase. The formula is as follows:

[0045] Log P = Log(C o / C w )

[0046] Among them, C o C represents the equilibrium concentration of a compound in a non-aqueous phase. w This indicates the equilibrium concentration of the compound in its neutral form in the aqueous phase. A larger Log P value indicates greater lipid solubility.

[0047] In some specific embodiments, the molecular weight of the modifier is 50-550, for example, it can be 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, and any range between these values.

[0048] In some specific embodiments, the modifier is a modifier containing hydroxyl, carboxyl, amino, glycoside, or amide structures. Examples of modifiers containing hydroxyl groups include glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, and trehalose. Examples of modifiers containing carboxyl groups include sodium lauroyl glutamate. Examples of modifiers containing amino groups include urea, pyrrolidone carboxylic acid and its salts, and amino acids and their salts, such as hydroxyproline and its salts, proline and its salts, and glutamic acid and its salts. Examples of modifiers containing glycoside structures include lauryl glucoside, raffinose, xylitol glucoside, and glyceryl glucoside. Examples of modifiers containing amide structures include glutamine and nicotinamide.

[0049] In some specific embodiments, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose.

[0050] In some specific embodiments, the mass ratio of chondroitin sulfate or its salt to the collagen substance is 1:(0.5-5), for example, it can be 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, and any range between these values.

[0051] In some specific embodiments, the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1 to 1:50, for example, it can be 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, and any range between these values.

[0052] In some specific embodiments, the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1 to 1:50, for example, it can be 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, and any range between these values.

[0053] In some specific embodiments, the mass ratio of chondroitin sulfate or its salt to the antioxidant component is 1:(0.1-30), for example, it can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, and any range between these values.

[0054] In one specific embodiment, the self-assembly composition further includes a solvent, which may be any solvent capable of dissolving the self-assembly composition, such as water or a water-containing solvent.

[0055] In some specific embodiments, the pH value of the self-assembled composition is greater than 4 and less than 7, for example, it can be 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, and any range between these values.

[0056] In some specific embodiments, the ionic strength of the self-assembled composition is <0.26 mol / kg, for example, it can be 0.25 mol / kg, 0.24 mol / kg, 0.23 mol / kg, 0.22 mol / kg, 0.21 mol / kg, 0.2 mol / kg, 0.19 mol / kg, 0.18 mol / kg, 0.17 mol / kg, 0.16 mol / kg, 0.15 mol / kg, 0.14 mol / kg, 0.13 mol / kg, 0.12 mol / kg, 0.11 mol / kg, 0.1 mol / kg, 0.09 mol / kg, 0.08 mol / kg, 0.07 mol / kg, 0.06 mol / kg, 0.05 mol / kg, 0.04 mol / kg, 0.03 mol / kg, 0.02 mol / kg, 0.01 mol / kg, and any range between these values.

[0057] In some specific embodiments, the self-assembled composition includes chondroitin sulfate or its salt, collagenous substances, and a modifier. The mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(0.5-5), the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50, the molecular weight of chondroitin sulfate or its salt is 20kDa-50kDa, the molecular weight of the collagenous substances is 10kDa-90kDa, the oil-water partition coefficient of the modifier is ≤4, the molecular weight is 50-550, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0058] In some specific embodiments, the self-assembled composition comprises chondroitin sulfate or its salt, collagenous substances, and a modifier. The mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(1-4), the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25, the molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagenous substances is 10kDa-90kDa, and the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose. The pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0059] In some specific embodiments, the self-assembled composition comprises chondroitin sulfate or its salt, collagenous substances, a modifier, and water. The mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(1-4), the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25, the molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagenous substances is 10kDa-90kDa, and the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose. The pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0060] In some embodiments, the self-assembly composition comprises chondroitin sulfate or its salts, collagen-like substances, modifiers, and water.

[0061] In some embodiments, the self-assembled composition comprises chondroitin sulfate or its salt, collagenous substances, a modifier, and water, wherein the mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(0.5-5), the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50, the molecular weight of chondroitin sulfate or its salt is 20kDa-50kDa, the molecular weight of the collagenous substances is 10kDa-90kDa, the oil-water partition coefficient of the modifier is ≤4, the molecular weight is 50-550, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0062] In some embodiments, the self-assembled composition comprises chondroitin sulfate or its salt, collagenous substances, a modifier, and water. The mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(1-4), the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25, the molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagenous substances is 10kDa-90kDa, and the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose. The pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26 mol / kg.

[0063] In some specific embodiments, the pH value of the cosmetic composition is greater than 4 and less than 7, for example, it can be 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, and any range between these values.

[0064] In some specific embodiments, the ionic strength of the cosmetic composition is <0.26 mol / kg, for example, it can be 0.25 mol / kg, 0.24 mol / kg, 0.23 mol / kg, 0.22 mol / kg, 0.21 mol / kg, 0.2 mol / kg, 0.19 mol / kg, 0.18 mol / kg, 0.17 mol / kg, 0.16 mol / kg, 0.15 mol / kg, 0.14 mol / kg, 0.13 mol / kg, 0.12 mol / kg, 0.11 mol / kg, 0.1 mol / kg, 0.09 mol / kg, 0.08 mol / kg, 0.07 mol / kg, 0.06 mol / kg, 0.05 mol / kg, 0.04 mol / kg, 0.03 mol / kg, 0.02 mol / kg, 0.01 mol / kg, and any range between these values.

[0065] In some specific embodiments, the cosmetic composition includes a self-assembled composition and an antioxidant component. The antioxidant component has a molecular weight < 450 and logP ≤ 2. The self-assembled composition includes chondroitin sulfate or its salt, collagen-like substances, and a modifier. The mass ratio of chondroitin sulfate or its salt to the collagen-like substances is 1:(0.5-5), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50. The molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa, and the molecular weight of the collagen-like substances is 10kDa-90kDa. The modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose. The pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is < 0.26 mol / kg.

[0066] In some specific embodiments, the cosmetic composition includes a self-assembled composition and an antioxidant component, wherein the antioxidant component has a molecular weight < 450 and logP ≤ 2; the self-assembled composition includes chondroitin sulfate or its salt, a collagen-like substance, and a modifier; the mass ratio of chondroitin sulfate or its salt to the collagen-like substance is 1:(1-4); the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25; and the mass ratio of chondroitin sulfate or its salt to the antioxidant component is... The ratio is 1:(0.1-30), the molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0067] In some specific embodiments, the cosmetic composition includes a self-assembled composition and an antioxidant component. The antioxidant component has a molecular weight of 100-350 and a logP of -3.5 to 2. The self-assembled composition includes chondroitin sulfate or its salt, collagen-like substances, and a modifier. The mass ratio of chondroitin sulfate or its salt to the collagen-like substances is 1:(1-4), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25. The mass ratio of the components is 1:(0.1-15), the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0068] In some specific embodiments, the cosmetic composition includes a self-assembled composition and an antioxidant component. The antioxidant component includes one or more of sodium bisulfite, ascorbic acid or its derivatives, ferulic acid, and ergothioneine. The self-assembled composition includes chondroitin sulfate or its salt, a collagen-like substance, and a modifier. The mass ratio of chondroitin sulfate or its salt to the collagen-like substance is 1:(0.5-5), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50. The mass ratio of the antioxidant component to the chondroitin sulfate is 1:(0.1-30), the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0069] In some specific embodiments, the cosmetic composition comprises a self-assembled composition and an antioxidant component.

[0070] In some specific embodiments, the cosmetic composition comprises a self-assembled composition and an antioxidant component, wherein the antioxidant component has a molecular weight <450 and logP≤2; the self-assembled composition includes chondroitin sulfate or its salt, collagenous substances, a modifier, and water; the mass ratio of chondroitin sulfate or its salt to the collagenous substances is 1:(0.5-5); the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50; the molecular weight of chondroitin sulfate or its salt is 20kDa-30kDa; the molecular weight of the collagenous substances is 10kDa-90kDa; the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose; the pH value of the composition is greater than 4 and less than 7; and the ionic strength of the composition is <0.26mol / kg.

[0071] In some specific embodiments, the cosmetic composition comprises a self-assembled composition and an antioxidant component, wherein the antioxidant component has a molecular weight < 450 and logP ≤ 2, and the self-assembled composition includes chondroitin sulfate or its salt, collagen-like substances, a modifier, and water, wherein the mass ratio of chondroitin sulfate or its salt to the collagen-like substances is 1:(1-4), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25. The mass ratio is 1:(0.1-30), the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0072] In some specific embodiments, the cosmetic composition comprises a self-assembled composition and an antioxidant component. The antioxidant component has a molecular weight of 100-350 and a logP of -3.5 to 2. The self-assembled composition includes chondroitin sulfate or its salt, collagen-like substances, a modifier, and water. The mass ratio of chondroitin sulfate or its salt to the collagen-like substances is 1:(1-4), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:25. The mass ratio of the chemical components is 1:(0.1-15), the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0073] In some specific embodiments, the cosmetic composition comprises a self-assembled composition and an antioxidant component. The antioxidant component includes one or more of sodium bisulfite, ascorbic acid or its derivatives, ferulic acid, and ergothioneine. The self-assembled composition comprises chondroitin sulfate or its salt, collagen-like substances, a modifier, and water. The mass ratio of chondroitin sulfate or its salt to the collagen-like substances is 1:(0.5-5), and the mass ratio of chondroitin sulfate or its salt to the modifier is 4:1-1:50. The mass ratio of its salt to the antioxidant component is 1:(0.1-30), the molecular weight of the chondroitin sulfate or its salt is 20kDa-30kDa, the molecular weight of the collagen-like substance is 10kDa-90kDa, the modifier is selected from one or more of glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose, the pH value of the composition is greater than 4 and less than 7, and the ionic strength of the composition is <0.26mol / kg.

[0074] In preparing the above-mentioned cosmetic composition, a self-assembled composition is first prepared, and then an antioxidant is added to the self-assembled composition to form the cosmetic composition. Specifically, in preparing the self-assembled composition, chondroitin sulfate or its salts, collagen-like substances, and modifiers are fully dissolved in a solvent. The order in which chondroitin sulfate or its salts, collagen-like substances, and modifiers are added is not limited, as long as they can be fully dissolved in the solvent.

[0075] In some specific embodiments, when preparing the self-assembled composition, chondroitin sulfate or its salts and collagenous substances are first fully dissolved in a solvent before the modifier is added. The pre-dissolution of chondroitin sulfate or its salts and collagenous substances facilitates the construction of a stable three-dimensional structure, which is beneficial for the formation of the self-assembled composition.

[0076] This application also provides the use of any of the above-described cosmetic compositions in preventing oxidation of the protected substance.

[0077] Example

[0078] The raw material information used in the following embodiments and comparative examples is shown in Table 1, and the equipment information is shown in Table 2.

[0079] Table 1

[0080]

[0081] Table 2

[0082]

[0083] Example 1

[0084] 1. Take a pre-sterilized 100ml beaker and accurately weigh chondroitin sulfate sodium, hydrolyzed collagen, modifier, and antioxidant using a precision balance. Dissolve chondroitin sulfate sodium and hydrolyzed collagen in water, add the modifier, and after complete dissolution, add the antioxidant. Stir until homogeneous to obtain the composition. In the composition, the content of chondroitin sulfate sodium is 0.4wt%, the content of hydrolyzed collagen is 0.8wt%, the content of modifier is 1wt%, and the content of antioxidant is 0.1wt%.

[0085] The modifier is glycerol, and the antioxidant is NaHSO3.

[0086] 2. Add a small magnetic stir bar to the beaker, seal the mouth of the beaker tightly with plastic wrap, and place it in a water bath at 600 rpm / 45℃ for 30 minutes until it is uniform, clear and transparent.

[0087] 3. Remove the beaker and let it cool to room temperature before use.

[0088] Examples 2-9

[0089] The only difference between Examples 2-9 and Example 1 is the type of modifier. Specifically, the modifier in Example 2 is sorbitol, in Example 3 it is pentylene glycol, in Example 4 it is xylitol, in Example 5 it is 1,2-hexanediol, in Example 6 it is trehalose, in Example 7 it is lauryl glucoside, in Example 8 it is sodium lauroyl glutamate, and in Example 9 it is raffinose.

[0090] Examples 10-13

[0091] The only difference between Examples 10-13 and Example 1 is the content of the modifier. Specifically, the modifier content in Example 10 is 0.1 wt%, in Example 11 it is 10 wt%, in Example 12 it is 15 wt%, and in Example 13 it is 20 wt%.

[0092] Examples 14-15

[0093] The only difference between Examples 14-15 and Example 3 is the content of the modifier. Specifically, the modifier content in Example 14 is 0.1 wt%, and the modifier content in Example 15 is 10 wt%.

[0094] Examples 16-18

[0095] The only difference between Examples 16-18 and Example 1 is the type of antioxidant. Specifically, the antioxidant in Example 16 is AA2G, the antioxidant in Example 17 is ferulic acid, and the antioxidant in Example 18 is ergothioneine.

[0096] Examples 19-23

[0097] The only difference between Examples 19-21 and Example 1 is the content of hydrolyzed collagen. Specifically, the hydrolyzed collagen content in Example 19 is 1.2 wt%, in Example 20 it is 0.4 wt%, in Example 21 it is 1.6 wt%, in Example 22 it is 0.2 wt%, and in Example 23 it is 2.0 wt%.

[0098] Examples 24-27

[0099] The only difference between Examples 24-27 and Example 1 is the content of the antioxidant. Specifically, the antioxidant content in Example 24 is 0.5 wt%, in Example 25 it is 1 wt%, in Example 26 it is 5 wt%, and in Example 27 it is 10 wt%.

[0100] Comparative Examples 11-3

[0101] The only difference between Comparative Examples 1-3 and Example 1 is the type of oxidant. Specifically, the oxidant in Comparative Example 1 is vitamin E, the oxidant in Comparative Example 2 is EGCG, and the oxidant in Comparative Example 3 is resveratrol.

[0102] Comparative Examples 4-10

[0103] Comparative Example 4 was a solution containing only 0.1 wt% NaHSO3, Comparative Example 5 was a solution containing only 0.1 wt% VE, Comparative Example 6 was a solution containing only 0.1 wt% EGCG, Comparative Example 7 was a solution containing only 0.1 wt% resveratrol, Comparative Example 8 was a solution containing only 0.1 wt% AA2G, Comparative Example 9 was a solution containing only 0.1 wt% ferulic acid, and Comparative Example 10 was a solution containing only 0.1 wt% ergothioneine.

[0104] The types and contents of each component in the above embodiments and comparative examples are shown in Table 3.

[0105]

[0106]

[0107] Experimental Example 1: Scanning Electron Microscopy

[0108] 1. Sample preparation

[0109] Take 10g of each sample from Table 3 and pour it into the groove of the pre-cleaned and dried sample container. Place the sample container in a freeze dryer and set the target temperature to -50℃ and the freeze-drying time to 24h.

[0110] 2. Testing Method (SEM)

[0111] (1) After disinfecting, cleaning and drying the sample stage, apply conductive adhesive to the sample stage.

[0112] (2) Use pre-cleaned tweezers to pick up the target sample and evenly adhere it to the conductive adhesive. Then use a bulb syringe to blow away any unadheded sample.

[0113] (3) After adding the samples in the order described above, label the samples with serial numbers to prevent confusion.

[0114] (4) Turn on the particle sputtering instrument, place the sample stage inside, and check the tightness of the cover and the air inlet valve. After that, start evacuating the vacuum. Wait until the vacuum level reaches between 2 and 10.

[0115] (5) Ensure the current value is between 5-15mA, automatically spray gold, and observe the sample surface color until it is close to grayish-black before stopping.

[0116] (6) Turn off the panel switch and restore it to its original state.

[0117] (7) Turn on the electron microscope's balance stage and place the sample stage (the highest point of the sample should not exceed the 5 mark, and should be as close as possible to facilitate subsequent imaging).

[0118] (8) Remove the sample chamber, place your left hand into the sample stage, and tighten the hex bolt with your right hand to close the sample chamber.

[0119] (9) Start vacuuming. After vacuuming is complete, turn on the electron gun and observe and adjust the filament current to between 50-53μA.

[0120] (10) Adjust, photograph, and record the SEM images of the sample.

[0121] (11) Turn off the electron gun and wait for the process to complete.

[0122] (12) Turn off the vacuum pump and start devastating.

[0123] (13) Open the sample chamber and remove the sample.

[0124] (14) Close the sample chamber and evacuate.

[0125] (15) Maintain internal vacuum and shut down the computer.

[0126] (16) After checking for any other potential hazards, turn off the instrument power.

[0127] The results show that the samples in this application all self-assembled to form chambers with small inner cavities and relatively uniform and smooth outer walls, effectively forming a spatial network structure that can prevent the material from being oxidized. The scanning electron microscope image of Example 7 is shown in Figure 1. Figure 1A It is a scanning electron microscope image magnified 100 times. Figure 1A It is a scanning electron microscope image magnified 500 times.

[0128] Experimental Example 2

[0129] Previous literature and patents typically use pigskin or 3D skin in vitro diffusion models to study the process by which antioxidants penetrate the skin and quench ROS within the skin, with the transdermal time of active ingredients often exceeding 8 hours. Considering the time interval between applying and washing off skincare products, antioxidant ingredients must maintain good activity for at least 8 hours after application. This application examines the protective effect against antioxidants over a period of 16 hours.

[0130] 1. Select a piece of faux leather (3cm*3cm) cut to the appropriate size, clean it thoroughly with 5% sodium lauryl sulfate, then wash it with water until completely dry, and set aside for use.

[0131] 2. Using a pipette, accurately transfer 1000 μL of the sample prepared in the examples and comparative examples onto a 2.5 cm * 2.5 cm circular area on the imitation leather.

[0132] 3. Place the coated imitation leather in a ventilated oven at 45℃ for 16 hours, then remove the imitation leather.

[0133] 4. Immerse the entire artificial leather in a beaker and add 20ml of deionized water. Place it in an ultrasonic bath at 25℃ and 30Hz for 30 minutes, then remove it.

[0134] 5. Titrate the sample using a 0.05% potassium permanganate solution.

[0135] 6. The protection rate parameter is defined as follows:

[0136] Antioxidant protection rate = V t / V0×100%

[0137] Among them, V t V0 represents the volume of potassium permanganate solution required for titrating a 1000 μL sample after 16 hours of aging, and V0 represents the volume of potassium permanganate solution required for titrating a freshly prepared 1000 μL sample.

[0138] The test results for the protection rate are shown in Table 3.

Claims

1. A cosmetic composition comprising a self-assembled composition and an antioxidant ingredient, The self-assembled composition comprises chondroitin sulfate or a salt thereof, type I hydrolyzed collagen, and a modifier. The modifier is selected from one or more of the following: glycerol, sorbitol, pentylene glycol, xylitol, 1,2-hexanediol, trehalose, lauryl glucoside, sodium lauroyl glutamate, and raffinose. The antioxidant components include one or more of sodium bisulfite, ascorbic acid or its derivatives, ferulic acid, and ergothioneine. The mass ratio of chondroitin sulfate or its salt to the type I hydrolyzed collagen is 1:(0.5-5). The mass ratio of chondroitin sulfate or its salt to the modifier is 4:1 to 1:50; The mass ratio of chondroitin sulfate or its salt to the antioxidant component is 1:(0.1-30). The pH value of the composition is greater than 4 and less than 7.

2. The composition according to claim 1, wherein the mass ratio of chondroitin sulfate or its salt to the type I hydrolyzed collagen is 1:(1-4).

3. The composition according to claim 1, wherein the mass ratio of chondroitin sulfate or its salt to the antioxidant component is 1:(0.1-15).

4. The composition according to claim 1, wherein the self-assembled composition further comprises a solvent.

5. The composition according to claim 1, wherein the ionic strength of the composition is <0.26 mol / kg.

6. Use of the composition according to any one of claims 1-5 in preventing the antioxidant component from being oxidized.

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