A kind of sugar-free granules of jasmine flower with anti-aging effect and preparation method thereof

Abstract

The present invention provides a kind of knot flower sugar-free granules with anti-aging effect and its preparation method, and relates to the field of medical technology. The knot flower sugar-free granules of the present invention are a composition of knot flower extract, mulberry extract, dextrin, carnosine, D-mannitol, xylitol, β-cyclodextrin, and microcrystalline cellulose, and the preparation method includes: cleaning and removing impurities from the knot flower, drying and crushing, extracting and concentrating to obtain the knot flower extract; cleaning and removing impurities from mulberries, extracting and concentrating to obtain the mulberry extract; mixing the knot flower extract, mulberry extract, dextrin, carnosine, D-mannitol, xylitol, β-cyclodextrin, and microcrystalline cellulose uniformly, placing in a wet granulator, and obtaining the knot flower sugar-free granules. The knot flower sugar-free granules prepared by the present invention have significant antioxidant and anti-sugar activities, have certain anti-aging effects, are widely applicable to the population, and have good market prospects.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to a jasmine flower sugar-free granule with anti-aging effect and a preparation method thereof. Background Art

[0002] Natural medicines have long been valued and recognized by people. Strengthening research on the evaluation of health benefits and the development of medicinal value of medicinal plants has far-reaching significance.

[0003] The genus Thymelaeaceae is widely distributed in the Yangtze River and the areas south of the Yangtze River. The edible and medicinal history of the edible and medicinal flowers of the edible and medicinal flowers in Henan, Guangxi and other regions is long, and it is one of the "wind" drugs in the classic Laoban Yao medicine. It tastes sweet, spicy, and warm in nature. It has the functions of nourishing yin and calming the mind, dispelling wind, and improving eyesight. Modern research shows that the edible and medicinal flowers contain many active substances, rich in variety, and have great health and medicinal value, mainly including: flavonoids, coumarins, steroids and organic acids and other active ingredients. Literature research reports that the crude extract of edible and medicinal flowers and monomer components such as Tiliroside have antibacterial, anti-glycation, and low-density lipoprotein oxidation inhibition effects. The petroleum ether and ethyl acetate extracts of edible and medicinal flowers have good antioxidant activity and can effectively scavenge hydroxyl radicals, ·OH radicals and nitrites. The ethyl acetate and n-butanol extracts of edible and medicinal flowers can inhibit the activity of α-glucosidase in vitro. Our research team's previous studies have verified that the flower of the jasmine contains a large amount of Tiliroside and similar ingredients. Tiliroside is a flavonoid component that has anti-glycation and antioxidant effects and may be an excellent natural anti-glycation agent. Therefore, the flower of the jasmine has the value and basis for in-depth research in anti-glycation and antioxidant aspects, and is of great value for in-depth development.

[0004] The jasmine is a national medicine and is also widely used among the people, but there has been a lack of systematic research on its medicinal and nutritional value. Its flower buds are directly dried and sold, with very low added value. Therefore, in order to develop the medicinal and nutritional value of jasmine flowers, increase the added value of jasmine-related products, and ensure stable employment of the labor force of jasmine growers, the present invention provides a jasmine flower sugar-free granule with anti-aging effect and a preparation method thereof. Summary of the invention

[0005] One of the purposes of the present invention is to provide a sugar-free granule of jasmine flower with anti-aging effect.

[0006] The second object of the present invention is to provide a method for preparing the sugar-free granules of jasmine flowers with anti-aging effect.

[0007] The sugar-free granules of jasmine flowers with anti-aging effects of the present invention are a composition of jasmine flower extract, mulberry extract, dextrin, D-mannitol, xylitol, carnosine, β-cyclodextrin and microcrystalline cellulose. Based on 100 parts by total weight, the sugar-free granules of jasmine flowers are prepared and mixed according to the following proportions: 20-40 parts of jasmine flower extract dry powder, 5-15 parts of mulberry extract dry powder, 6-9 parts of D-mannitol, 0.5-1 part of xylitol, 5-8 parts of β-cyclodextrin, 0.08-0.16 parts of carnosine, 1-3 parts of microcrystalline cellulose, and the balance is dextrin.

[0008] The method for preparing the anti-aging granules of jasmine flowers comprises the following steps:

[0009] 1. Preparation of jasmine flower extract

[0010] ①Put the raw materials of the jasmine flowers in a sealed container and wash them 2 to 3 times in clean water at 4 to 15 degrees Celsius.

[0011] During the cleaning process, ozone is injected, and each cleaning time is 5 to 10 minutes. Then the flowers are freeze-dried and crushed through an 80-100 mesh sieve.

[0012] ② Add 5~10 times the volume of 50~80% ethanol and soak for 1~2 hours, then heat to 65~85℃ for reflux extraction

[0013] 2~3 times, each time for 1~2.5h, combine the extracts from each time, recover ethanol under reduced pressure, and then concentrate to obtain a concentrated solution; keep the remaining filter residue after alcohol extraction for later use;

[0014] ③ After alcohol extraction, add 6 to 8 times the volume of ethyl acetate to the concentrated solution and extract 2 to 3 times to obtain ethyl acetate.

[0015] Extraction and separation fluids;

[0016] ④ Add 5-10 times the total weight of the raw materials to the residue remaining after alcohol extraction and heat to 100-110°C

[0017] Reflux extraction 2-3 times, each extraction 1-2h, combine each water extract and collect for later use;

[0018] ⑤ The water extract, ethyl acetate extract and separation liquid are concentrated, dried, powdered, and then mixed.

[0019] Obtain the dried powder of jasmine flower extract.

[0020] 2. Preparation of mulberry extract

[0021] The mulberry raw material is washed 2-3 times in clean water at 4-15°C, and ozone is added during each washing process, and the washing time is 5-10 minutes; then 4-8% lemon juice is added to the mulberry and beaten into a paste; at 4-10°C, 10-15 times the volume of 1-3% citric acid ethanol solution is added to the mulberry paste for leaching for 6-8 hours, the extract is filtered, the filter residue is recovered and the extraction is repeated 2-3 times, the extracts from each time are combined, ethanol is recovered under reduced pressure, and then concentrated to obtain a concentrated solution, which is dried and crushed to obtain a mulberry extract powder.

[0022] 3. Preparation of sugar-free granules of jasmine flowers

[0023] Weigh the dried powder of jasmine flower extract, the dried powder of mulberry extract, dextrin, D-mannitol, xylitol, β-cyclodextrin, carnosine and microcrystalline cellulose, mix them evenly, sieve them 2-3 times and mix them evenly; then add ethanol with a concentration of 75-90% in a spray stirring manner, with a solid-liquid ratio of 2.5-4:1, use a wet granulator to granulate for 2-3 minutes, and dry them to obtain the jasmine flower sugar-free granules.

[0024] Preferably, based on 100 parts by total weight, the sugar-free granules of knotweed flower are prepared and mixed according to the following proportions: 20-40 parts of knotweed flower extract dry powder, 5-15 parts of mulberry extract dry powder, 6-9 parts of D-mannitol, 0.5-1 part of xylitol, 5-8 parts of β-cyclodextrin, 0.08-0.16 parts of carnosine, 1-3 parts of microcrystalline cellulose, and the balance is dextrin.

[0025] The antioxidant capacity was compared by comparing the scavenging ability of DPPH free radicals and ·OH, and the anti-glycation effect was screened using an in vitro protein non-enzymatic glycosylation simulation reaction system (BSA-fructose and BSA-MGO). The health benefits of the antioxidant and anti-glycation effects of the jasmine flower were evaluated.

[0026] Determination of DPPH free radical scavenging ability

[0027] Use anhydrous ethanol to prepare the sample solution with a concentration of 0.20 mg / mL for standby use, dilute VC with distilled water to the same concentration and store in the dark. Add 2 mL of 80% methanol solution and 3 mL of 0.5 mmol / L DPPH methanol solution to the blank tube; add 2 mL of sample solution and 3 mL of 0.5 mmol / L DPPH methanol solution to the measuring tube; add 2 mL of sample solution and 3 mL of 80% methanol solution to the control tube, mix well, stand at room temperature in the dark for 30 min, measure the absorbance at 517 nm, and adjust to zero with anhydrous ethanol. The calculation formula for DPPH free radical scavenging rate is:

[0028] DPPH free radical scavenging rate (%) = [1-(A sample-A control) / A blank] × 100%

[0029] Wherein: Asample is the absorbance value of the measuring tube at a wavelength of 517 nm; Acontrol is the absorbance value of the control tube at a wavelength of 517 nm; Ablank is the absorbance value of the blank tube at a wavelength of 517 nm.

[0030] Hydroxyl free radical scavenging ability assay

[0031] The Fenton reaction was used to determine the scavenging capacity of the sample for ·OH, and VC was used as the control for the test. Test solution preparation: The sample was prepared with anhydrous ethanol to a concentration of 1.0 mg / mL for later use, and VC was diluted with distilled water to the same concentration and stored in the dark. Add 2.0 mL of 0.2 mol / L phosphate buffer solution and 1.5 mL of o-phenanthroline to the tube, add 1.0 mL of 7.5 mmol / L ferrous sulfate and 5.5 mL of distilled water to the blank tube, add 1.0 mL of 7.5 mmol / L ferrous sulfate, 4.5 mL of distilled water, and 1.0 mL of 0.1% H2O2 solution to the control tube, and add 2.0 mL of 7.5 mmol / L ferrous sulfate, 1.0 mL of sample solution, 2.5 mL of distilled water, and 1.0 mL of 0.1% H2O2 solution to the measurement tube. Mix the above tubes, keep each tube at 37 °C for 20 min, measure the absorbance at 510 nm, and adjust to zero with distilled water. The calculation formula for OH scavenging rate is:

[0032] OH scavenging rate (%) = [1-(A sample-A control) / A blank] × 100 %

[0033] Wherein: Asample is the absorbance value of the measuring tube at a wavelength of 510 nm; Acontrol is the absorbance value of the control tube at a wavelength of 510 nm; Ablank is the absorbance value of the blank tube at a wavelength of 510 nm.

[0034] Anti-glycation activity assay method

[0035] The reaction mixture was 20 mL in total and consisted of bovine serum albumin (BSA) (20 mg / mL), glucose (500 mmol / L), sodium azide (1%), phosphate buffer (200 mmol / L, pH 7.4) and the sample to be tested (0.5 mg / mL). Phosphate buffer was added instead of the sample to be tested as a negative control, and aminoguanidine (AG) was added instead of the sample to be tested as a positive control. After all the mixtures were stored at 37±0.5℃ for 20 days, 1.5 mL of saccharification solution was taken from the entire mixture for the following experiment.

[0036] Dilute 0.5 ml of saccharification solution to 5 ml with phosphate buffer (200 mmol / L, pH 7.4), measure the fluorescence intensity, set the excitation wavelength to 370 nm, and set the emission wavelength to 440 nm. The inhibition rate of polysaccharide on the formation of fluorescent AGEs (%) = (A2A1) / A2 × 100%, where A1: fluorescence intensity of the sample group to be tested, A2: fluorescence intensity of the sample blank group. Beneficial Effects

[0037] The present invention first uses ozone cleaning to largely remove dust, agricultural residues and microorganisms on the surface of the jasmine flower, which is beneficial to improving the quality of the finished product; secondly, the present invention adds granules composed of three active ingredients, namely, jasmine flower extract, mulberry extract and carnosine, wherein the jasmine flower extract has significant antioxidant and anti-glycation effects and has a certain anti-aging effect. Carbonylated proteins and advanced glycation end products (AGEs) are signals of cell aging. The most preferentially glycosylated sites are amino groups and carbonyl groups near imidazole, and some active ingredients contained in the jasmine flower extract happen to have these groups. Therefore, after adding the jasmine flower extract, it has the highest priority for reaction and replaces the protein to be glycosylated in advance, thereby helping to protect the protein from being glycosylated and achieving the effect of anti-glycation. In addition, flavonoids such as rutin, linalool, kaempferol-3-O-pyranoglucoside contained in the jasmine flower extract have strong antioxidant activity. The anthocyanins contained in the mulberry extract also have a scavenging and inhibitory effect on .OH and DPPH. Carnosine has a significant inhibitory effect on lipid oxidation caused by free radicals and metal ions. Carnosine also has a good anti-glycation effect. Therefore, under the synergistic effect of mulberry extract and carnosine, the antioxidant and anti-glycation effects of the jasmine flower sugar-free granules are more significant.

[0038] The sugar-free granules of osmanthus flowers with anti-aging effects prepared by the invention have remarkable effects in anti-glycation and anti-oxidation, improve the medicinal and nutritional value of osmanthus flowers, increase the added value of osmanthus-related products, ensure stable employment of the labor force of osmanthus growers, and have excellent market prospects. DETAILED DESCRIPTION

[0039] The jasmine flowers of the present invention were purchased from Tibet Yuewang Biotechnology Co., Ltd.; mulberries were purchased from Hefei Zhougudui Fruit Wholesale Market; D-mannitol, xylitol, β-cyclodextrin, microcrystalline cellulose and dextrin were purchased from Anhui Yuanzheng Bioengineering Co., Ltd.; and carnosine was purchased from Shanghai Kolaman Reagent Co., Ltd.

[0040] The present invention is further explained below in conjunction with specific implementation examples. It should be understood that these embodiments are only used to illustrate the present invention but not to limit the scope of the present invention. After reading the present invention, various equivalent modifications of the present invention by technicians in this field all fall within the scope specified by the claims attached to this application. Example

[0041] The purpose of the present invention is to provide a sugar-free granule of jasmine flower with anti-aging effect and a preparation method thereof, comprising the following steps:

[0042] 1. Preparation of jasmine flower extract

[0043] ①Put the raw materials of the jasmine flowers in a sealed container and wash them twice in clean water at 4°C.

[0044] Fill with ozone, and each cleaning time is 5 minutes; then freeze-dry the jasmine flowers and crush them through an 80-mesh sieve;

[0045] ② Add 5 times the volume of 50% ethanol and soak for 2 hours, then heat to 85℃ and reflux for extraction 3 times, each time for 1 hour.

[0046] Combine the extracts from each extraction, recover the ethanol under reduced pressure, and then concentrate to obtain a concentrated solution; keep the filter residue left after the alcohol extraction for later use;

[0047] ③ After alcohol extraction, add 6 times the volume of ethyl acetate to the concentrated solution and extract twice to obtain ethyl acetate extract.

[0048] and separation fluid;

[0049] ④ Add 5 times the total weight of raw materials to the residue left after alcohol extraction, heat to 100℃ and reflux for extraction

[0050] 2 times, each time for 1 hour, combine each water extract and collect for later use;

[0051] ⑤ The water extract, ethyl acetate extract and separation liquid are concentrated, dried, powdered, and then mixed.

[0052] Obtain the dried powder of jasmine flower extract.

[0053] 2. Preparation of mulberry extract

[0054] The mulberry raw material was washed twice in clean water at 4°C, and ozone was added during each washing process, and the washing time was 5 minutes each time; then 4% lemon juice was added to the mulberry and beaten into a paste; at 4°C, 10 times the volume of 1% citric acid ethanol solution was added to the mulberry paste and extracted for 6 hours, the extract was filtered, the filter residue was recovered and the extraction was repeated 3 times, the extracts from each time were combined, ethanol was recovered under reduced pressure, and then concentrated to obtain a concentrated solution, which was dried and crushed to obtain a mulberry extract powder.

[0055] 3. Preparation of sugar-free granules of jasmine flowers

[0056] Weigh 20 g of jasmine flower extract powder, 5 g of mulberry extract powder, 61.9 g of dextrin, 6 g of D-mannitol, 1 g of xylitol, 5 g of β-cyclodextrin, 0.08 g of carnosine and 1 g of microcrystalline cellulose, mix them evenly, sieve them twice and mix them evenly; then add 75% ethanol in a spray stirring manner with a solid-liquid ratio of 4:1, use a wet granulator to granulate for 2 minutes, and dry them to obtain the jasmine flower sugar-free granules. Example

[0057] 1. Preparation of jasmine flower extract

[0058] ①Put the raw materials of the jasmine flowers in a sealed container and wash them three times in clean water at 10°C.

[0059] Fill with ozone, and each cleaning time is 8 minutes; then freeze-dry the jasmine flowers and crush them through a 90-mesh sieve;

[0060] ② Add 8 times the volume of 65% ethanol and soak for 1.5 hours, then heat to 75℃ and reflux for extraction 3 times, each time

[0061] 2h, combine the extracts from each extraction, recover the ethanol under reduced pressure, and then concentrate to obtain a concentrated solution; the remaining filter residue after alcohol extraction is reserved for later use;

[0062] ③ After alcohol extraction, add 7 times the volume of ethyl acetate to the concentrated solution and extract 3 times to obtain ethyl acetate extract.

[0063] and separation fluid;

[0064] ④ Add 8 times the total weight of raw materials of water to the residue left after alcohol extraction, heat to 105℃ and reflux for extraction

[0065] 3 times, each time for 2 hours, combine each water extract and collect for later use;

[0066] ⑤ The water extract, ethyl acetate extract and separation liquid are concentrated, dried, powdered, and then mixed.

[0067] Obtain the dried powder of jasmine flower extract.

[0068] 2. Preparation of mulberry extract

[0069] The mulberry raw material was washed three times in clean water at 10°C, and ozone was added during each washing process, and the washing time was 8 minutes each time; then 6% lemon juice was added to the mulberry and beaten into a paste; at 8°C, 12 times the volume of 2% citric acid ethanol solution was added to the mulberry paste and extracted for 7 hours, the extract was filtered, the filter residue was recovered and the extraction was repeated three times, the extracts from each time were combined, ethanol was recovered under reduced pressure, and then concentrated to obtain a concentrated solution, which was dried and crushed to obtain a mulberry extract powder.

[0070] 3. Preparation of sugar-free granules of jasmine flowers

[0071] Weigh 30 g of jasmine flower extract powder, 10 g of mulberry extract powder, 42.1 g of dextrin, 8 g of D-mannitol, 0.8 g of xylitol, 7 g of β-cyclodextrin, 0.1 g of carnosine and 2 g of microcrystalline cellulose, mix them evenly, sieve them 3 times and mix them evenly; then add 80% ethanol in a spray stirring manner with a solid-liquid ratio of 3:1, use a wet granulator to granulate for 3 minutes, and dry them to obtain the jasmine flower sugar-free granules. Example

[0072] 1. Preparation of jasmine flower extract

[0073] ①Put the raw materials of the jasmine flowers in a sealed container and wash them three times in clean water at 15℃.

[0074] Fill with ozone, and each cleaning time is 10 minutes; then freeze-dry the jasmine flowers and crush them through a 100-mesh sieve;

[0075] ② Add 10 times the volume of 80% ethanol and soak for 2 hours, then heat to 65℃ and reflux for extraction 3 times, each time

[0076] 2.5h, combine the extracts from each extraction, recover the ethanol under reduced pressure, and then concentrate to obtain a concentrated solution; the remaining filter residue after alcohol extraction is reserved for later use;

[0077] ③ After alcohol extraction, add 6 to 8 times the volume of ethyl acetate to the concentrated solution and extract 2 to 3 times to obtain ethyl acetate.

[0078] Extraction and separation fluids;

[0079] ④ Add 5 to 10 times the total weight of the raw materials to the residue remaining after alcohol extraction and heat to 100~

[0080] Reflux extraction at 110℃ for 2-3 times, each extraction for 1-2h, combine the water extracts from each extraction and collect for later use;

[0081] ⑤ The water extract, ethyl acetate extract and separation liquid are concentrated, dried, powdered, and then mixed.

[0082] Obtain the dried powder of jasmine flower extract.

[0083] 2. Preparation of mulberry extract

[0084] The mulberry raw material was washed three times in clean water at 15°C, and ozone was added during each washing process. The washing time was 10 minutes each time. Then, 8% lemon juice was added to the mulberry and beaten into a paste. At 10°C, 15 times the volume of 3% citric acid ethanol solution was added to the mulberry paste and extracted for 8 hours. The extract was filtered, and the residue was recovered and extracted twice. The extracts from each time were combined, ethanol was recovered under reduced pressure, and then concentrated to obtain a concentrated solution, which was dried and crushed to obtain a mulberry extract powder.

[0085] 3. Preparation of sugar-free granules of jasmine flowers

[0086] Weigh 40 g of jasmine flower extract powder, 15 g of mulberry extract powder, 24.3 g of dextrin, 9 g of D-mannitol, 0.5 g of xylitol, 8 g of β-cyclodextrin, 0.16 g of carnosine and 3 g of microcrystalline cellulose, mix them evenly, sieve them 3 times and mix them evenly; then add 90% ethanol in a spray stirring manner with a solid-liquid ratio of 2.5:1, use a wet granulator to granulate for 3 minutes, and dry them to obtain the jasmine flower sugar-free granules.

[0087] Control 1: Weigh 40 g of dried powder of jasmine flower extract, 39.4 g of dextrin, 9 g of D-mannitol, 0.5 g of xylitol, 8 g of β-cyclodextrin and 3 g of microcrystalline cellulose, mix them evenly, sieve them 3 times and mix them evenly; then add 90% ethanol by spraying and stirring, with a solid-liquid ratio of 2.5:1, use a wet granulator to granulate for 3 minutes, and dry to obtain granules.

[0088] Control 2: Weigh 15 g of mulberry extract powder, 64.3 g of dextrin, 9 g of D-mannitol, 0.5 g of xylitol, 8 g of β-cyclodextrin, 0.16 g of carnosine, and 3 g of microcrystalline cellulose, mix them evenly, sieve them 3 times, and mix them evenly; then add 90% ethanol in a spray stirring manner, with a solid-liquid ratio of 2.5:1, use a wet granulator to granulate for 3 minutes, and dry to obtain granules.

[0089] Blank control: weigh 79.4 g of dextrin, 9 g of D-mannitol, 0.5 g of xylitol, 8 g of β-cyclodextrin, and 3 g of microcrystalline cellulose, mix them evenly, sieve them 3 times, and mix them evenly; then add 90% ethanol by spraying and stirring, with a solid-liquid ratio of 2.5:1, use a wet granulator to granulate for 3 minutes, and dry to obtain granules.

[0090] Comparison and results of each indicator:

[0091] Control group 1 was a granule without mulberry extract and carnosine; control group 2 was a granule without jasmine flower extract; and the blank control group was a granule without jasmine flower extract, mulberry extract, and carnosine. The antioxidant capacity was compared by comparing the scavenging ability of DPPH free radicals and ·OH in each experimental group, and the anti-glycation effect was screened by in vitro protein non-enzymatic glycosylation simulation reaction system (BSA-fructose and BSA-MGO), and the health care efficacy of jasmine flower was evaluated for its antioxidant and anti-glycation effects.

[0092] Table 1 Comparison of indicators between the embodiment and the control group

[0093] DPPH free radical scavenging rate (%) OH removal rate (%) Glycation inhibition rate (%) Example 1 73.94% 68.29% 20.15% Example 2 75.30% 65.21% 22.46% Example 3 79.28% 69.48% 24.83% Control group 1 58.16% 42.23% 13.47% Control group 2 22.53% 19.86% 6.29% Blank control 3.17% 1.07% 0.14%

[0094] The test results show that the three groups of examples have the most significant antioxidant and anti-glycation effects, among which the DPPH free radical scavenging rate and ·OH scavenging rate can reach 79.28% and 69.48% respectively. The possible reason is that the flavonoids such as rutin, linalool, kaempferol-3-O-pyranoglucoside contained in the jasmine flower extract have strong antioxidant activity, and combined with mulberry extract and carnosine can more effectively scavenge DPPH free radicals and ·OH, enhancing its in vitro antioxidant activity. At the same time, the jasmine flower extract has a more significant inhibitory effect on the advanced glycation products (AGEs), which is higher than the AGEs inhibition rate of aminoguanidine AG at the same concentration (19.58%), and significantly higher than other control groups, indicating that some active ingredients contained in the jasmine flower extract have amino groups and carbonyl groups close to imidazole, which can replace proteins in the anti-glycation reaction and be preferentially glycosylated, thereby helping to protect proteins from being glycosylated and achieving the effect of anti-glycation.

[0095] The sugar-free granules of jasmine flowers with anti-aging effects prepared by the invention have remarkable effects in anti-saccharification and anti-oxidation, have certain anti-aging effects, improve the medicinal and nutritional values ​​of jasmine flowers, and increase the added value of jasmine flowers.

Claims

1. A sugar-free granule of jasmine flower with anti-aging effect, characterized in that: Based on 100 parts by total weight, the sugar-free granules of osmanthus fragrans are prepared and mixed according to the following proportions: 20-40 parts of osmanthus fragrans extract dry powder, 5-15 parts of mulberry extract dry powder, 6-9 parts of D-mannitol, 0.5-1 part of xylitol, 5-8 parts of β-cyclodextrin, 0.08-0.16 parts of carnosine, 1-3 parts of microcrystalline cellulose, and the balance is dextrin.

2. A method for preparing a sugar-free granule of jasmine flower with anti-aging effect, characterized in that: The preparation method comprises the following steps: A. Preparation of Fragrant Flower Extract ① Place the raw materials of jasmine flowers in a sealed container, wash, freeze-dry, and crush through a 80-100 mesh sieve; ② Add 5-10 times the volume of 50-80% ethanol and soak for 1-2 hours, heat to 65-85℃ and reflux extract 2-3 times, each time for 1-2.5 hours, combine each extract, recover ethanol under reduced pressure, and then concentrate to obtain a concentrated solution; keep the remaining filter residue after alcohol extraction for later use; ③ After the alcohol extraction, add 6 to 8 times the volume of ethyl acetate to the concentrated solution and extract 2 to 3 times to obtain ethyl acetate extract and separation solution; ④ Add 5-10 times the total weight of the raw materials to the residue after alcohol extraction, heat to 100-110℃ and reflux for 2-3 times, each time for 1-2h, combine each water extract and collect for later use; ⑤ The water extract, ethyl acetate extract and separation liquid are concentrated, dried, powdered, and then mixed to obtain the dried powder of the jasmine flower extract; B. Preparation of mulberry extract Wash the mulberry raw material; then add 4-8% lemon juice to the mulberry and beat them into a paste; add 10-15 times the volume of 1-3% citric acid ethanol solution to the mulberry paste at 4-10°C for 6-8h, filter the extract, recover the filter residue and repeat the extraction 2-3 times, combine the extracts each time, recover ethanol under reduced pressure, and then concentrate to obtain a concentrated solution, which is dried and crushed to obtain a mulberry extract powder; C. Preparation of sugar-free granules of jasmine flowers Weigh the dried powder of jasmine flower extract, the dried powder of mulberry extract, dextrin, D-mannitol, xylitol, β-cyclodextrin, carnosine and microcrystalline cellulose, mix them evenly, sieve them 2-3 times and mix them evenly; then add 75-90% ethanol in a spray stirring manner, with a solid-liquid ratio of 2.5-4:1, granulate for 2-3 minutes, and dry them to obtain the jasmine flower sugar-free granules; Among them, based on a total weight of 100 parts, the described jasmine flower sugar-free granules are prepared and mixed according to the following proportions: 20-40 parts of jasmine flower extract dry powder, 5-15 parts of mulberry extract dry powder, 6-9 parts of D-mannitol, 0.5-1 part of xylitol, 5-8 parts of β-cyclodextrin, 0.08-0.16 parts of carnosine, 1-3 parts of microcrystalline cellulose, and the balance is dextrin.

3. The method for preparing the sugar-free granules of jasmine flowers with anti-aging effect according to claim 2, characterized in that: The cleaning method of the jasmine flower and mulberry raw materials is: cleaning them in clean water at 4-15°C for 2-3 times, filling with ozone during each cleaning process, and each cleaning time is 5-10 minutes.