Active component of a codling moth trapping agent, trapping agent and trapping method

Abstract

The present application provides a kind of pear fruit small moth trapping agent active component: sex attractant, it includes cis-8-dodecene alcohol acetate, trans-8-dodecene alcohol acetate, cis-8-dodecene alcohol and dodecane-1-alcohol;And oviposition attractant, it includes benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexene butyric acid ester, cis-3-hexenol;The volume ratio of the sex attractant and the oviposition attractant is 10:1.The present application also provides a kind of pear fruit small moth trapping agent, which comprises active component and non-polar organic solvent.The present application also provides a kind of pear fruit small moth trapping method.The present application can simultaneously attract different reproductive state female and male insects, and can be applied to pear fruit small moth situation forecasting, mass trapping and mating interference.

Description

Technical Field

[0001] This invention belongs to the field of pest control, and in particular relates to an active component of a pear fruit moth trap, the trap, and a trapping method. Background Technology

[0002] The pear fruit moth (Grapholitha molesta) (Busck) is a global fruit-boring pest that infests the tender shoots and fruits of various Rosaceae fruit trees, such as apples, peaches, pears, apricots, and cherries, causing damage such as "twig breakage," "black plaster," and "sand-like" appearance. Infested fruits are prone to falling off, rotting, and poor storage. With the widespread distribution of its host plants, this insect is found in major deciduous fruit orchards worldwide. Because the larvae of the pear fruit moth are concealed and have a short exposure time, they are difficult to control; therefore, the adult and egg stages are the primary control periods.

[0003] Long-term use of chemical pesticides to control the pear fruit moth not only leads to pesticide resistance and unstable control effects, but also causes a series of problems such as food safety issues, harm to natural enemies, and damage to the ecosystem. Pest olfactory behavior regulation technology is currently a hot topic in green pest control. Sex pheromones are specific and can effectively attract and kill males and disrupt mating, making them one of the most effective measures for controlling the pear fruit moth. The host transfer and spread of egg-laying by fertile females is a significant reason for the increased damage caused by the pear fruit moth in later stages of pear orchard life. Meanwhile, males exhibit multiple mating characteristics, and females prefer males that have already mated once. Males and females at different reproductive stages respond differently to pheromone compounds; therefore, using sex pheromones solely to attract males is insufficient to address the sexual reproduction characteristics of the pear fruit moth. Previous studies have confirmed that host plant volatiles have a strong attraction to both male and female pear fruit moths, enhancing the response of fertile females and promoting egg-laying. Host plant volatiles have a synergistic effect on insect sex attractants, and when used in combination with insect sex attractants, they can further enhance the control effect on pests. Summary of the Invention

[0004] One object of the present invention is to provide a trap and method for trapping the pear fruit moth, and to provide at least the advantages described below.

[0005] Another objective of this invention is to provide a trap and trapping method for the pear fruit moth, which can simultaneously attract male and female moths in different reproductive stages. It can be applied to pest monitoring, mass trapping, and interference with mating of the pear fruit moth.

[0006] The technical solution of the present invention is as follows:

[0007] The active components of the pear fruit moth trap include:

[0008] Sex attractants, including cis-8-dodecenoyl acetate, trans-8-dodecenoyl acetate, cis-8-dodecenoyl alcohol, and dodecano-1-ol;

[0009] And oviposition attractants, including benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol;

[0010] The volume ratio of the sex attractant to the oviposition attractant is 10:1.

[0011] Preferably, the active component of the pear fruit moth trap contains,

[0012] The mass ratio of cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecyl-1-ol is 100:6.8:19.1:5.4.

[0013] The mass ratio of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl butyrate, and cis-3-hexenol is 100:1:100:1000:10 or 100:1000:10:1:100.

[0014] A pear fruit moth trap, comprising a pear fruit moth trap active ingredient and n-hexane;

[0015] in,

[0016] The active components of the pear fruit moth trap include a sex attractant and an oviposition attractant in a volume ratio of 10:1. The sex attractant includes cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodec-1-ol. The oviposition attractant includes benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol.

[0017] The method for trapping the pear fruit moth includes the following steps:

[0018] The pear fruit moth trap was prepared by adding the active components of the trap to n-hexane. The active components of the pear fruit moth trap included a sex attractant and an oviposition attractant in a volume ratio of 10:1. The sex attractant included cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecenol-1-ol. The oviposition attractant included benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol.

[0019] A lure core is prepared by adding a pear fruit moth trap into a centrifuge tube with a rubber stopper or a polypropylene plastic centrifuge tube.

[0020] The lure is placed on the trap and hung on the pear tree for trapping;

[0021] in;

[0022] The mass ratio of cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecyl-1-ol is 100:6.8:19.1:5.4.

[0023] The mass ratio of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenobutyrate, and cis-3-hexenool is 100:1:100:1000:10 or 100:1000:10:1:100.

[0024] The concentration of the sex attractant is 13.1 μg / μL, and the concentration of the oviposition attractant is 5 μg / μL.

[0025] Preferably, in the method for trapping the pear fruit moth, the trap is suspended at a height of 1.5 m and at intervals greater than 30 m.

[0026] This invention can elicit a strong olfactory response in both male and female or pear fruit moths at different reproductive stages, especially in oviparous females and males that have just mated. The combination of sex pheromones and oviposition pheromones effectively increases the number of adult or pear fruit moths captured in the field and reduces the likelihood of damage from offspring. This pheromone composition can simultaneously monitor pest populations, trap large numbers of orphans, and disrupt mating and oviposition in both males and females. Furthermore, it is more targeted at the key reproductive stages of both sexes, resulting in better control and promoting the development of sex pheromones.

[0027] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0028] Figure 1 A graph showing the EAG response of female insects in different reproductive stages to oviposition attractants;

[0029] Figure 2 The diagram shows the EAG response results of male insects in different reproductive stages to sex pheromones and oviposition pheromones.

[0030] Figure 3 The diagram shows the trapping effect of different proportions of attractants and oviposition attractants on the pear fruit moth in the field. Detailed Implementation

[0031] The present invention will now be described in further detail so that those skilled in the art can implement it based on the description.

[0032] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not imply the presence or addition of one or more other elements or combinations thereof.

[0033] The test volatiles were dissolved in n-hexane to prepare sex attractant solutions with a concentration of 13.1 μg / μL and oviposition attractant solutions with a concentration of 5 μg / μL. Example 1:

[0034] The pear fruit moth trap contains only one oviposition attractant component at a concentration of 5 μg / μL. The trap is a triangular trap, set up in the field at intervals of approximately 30 m and suspended at a height of approximately 1.5 m. The experimental results are shown in Table 1.

[0035] The experimental results show that benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl acetate, and cis-3-hexenol have higher trapping effects than other components.

[0036] Table 1. Trapping capacity of a single component of the spawning attractant

[0037] Example 2:

[0038] Based on Example 1, the oviposition attractant components in the pear fruit moth trap consist of five elements: benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl acetate, and cis-3-hexenol, with a concentration of 5 μg / μL and contents of 1, 10, 100, or 1000 μL, as shown in Table 2.

[0039] Table 2. Contents of Components in the Ovulation Attractant Mixture (Orthogonal Design)

[0040]

[0041] The traps were triangular traps, set up in the field at intervals of about 30 m and suspended at a height of about 1.5 m. The test results are shown in Table 3.

[0042] The experimental results show that the trapping effect of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl acetate, and cis-3-hexenol is higher than that of other combinations when the contents are 100, 1, 100, 1000, 10 μL and 100, 1000, 10, 1, 100 μL, respectively.

[0043] Table 3. Trapping capacity of mixed components of spawning attractant

[0044] Example 3:

[0045] EAG responses of male and female insects in different reproductive stages to sex pheromones and oviposition pheromones

[0046] Healthy adult insects with intact antennae were selected for testing.

[0047] The entire antenna of the adult insect was cut off from the base using a scalpel blade, with a small portion of the tip removed. It was then fixed to a metal electrode using conductive adhesive, and the electrode was connected to a signal amplifier via a silver wire. The antennal potentiometer was adjusted to a continuous gas flow rate of 200 mL / min and a pulsed stimulation gas flow rate of 20 mL / min. Measurements were started after the baseline stabilized.

[0048] Add 10 μl of sex pheromone solution or oviposition pheromone solution to a filter paper strip, place it into a clean 1000 μL pipette tip, and connect the tip to the gas stimulation control device of the antennal potentiometer. The pulse interval-stimulation duration is 0.3 s, with a 1 min interval between stimulations. Each treatment is tested once on the same antenna, receiving two consecutive stimulations, testing all six antennae, using only one antenna per adult insect. The solvent-induced EAG relative response value serves as a control, and measurements are performed alternately between the control and treatment groups.

[0049] like Figure 1 As shown, the EAG response values ​​of unmated females to the oviposition attractant were significantly higher than those of the n-hexane control (t=6.791, df=24, P<0.05), the EAG response values ​​of gestating females to the oviposition attractant were significantly higher than those of the n-hexane control (t=11.931, df=32, P<0.05), and the EAG response values ​​of oviposition females to the oviposition attractant were significantly higher than those of the n-hexane control (t=5.521, df=24, P<0.05).

[0050] Significant differences were found in the EAG response values ​​of female insects at different reproductive stages to the oviposition attractant (F=11.447, df=2.42, P<0.05), with gestating females showing significantly higher EAG response values ​​than unmated and oviposition females. No significant differences were found in the EAG response values ​​of female insects at different reproductive stages to the n-hexane control (F=0.358, df=2.42, P=0.701).

[0051] like Figure 2 As shown, there were significant differences in EAG response values ​​of male insects in different reproductive states to sex pheromones, oviposition pheromones, and n-hexane controls (F=140.633, df=2, P<0.05). Among them, the EAG response value of sex pheromones was the highest, followed by oviposition pheromones, and the EAG response value of n-hexane controls was the lowest.

[0052] Significant differences were found in the EAG response values ​​of unmated males, males that had mated once, and males that had mated twice after stimulation with different attractants (F=10.455, df=2, P<0.05). Among them, the EAG response value of males that had mated once was significantly higher than that of unmated males and males that had mated twice. Example 4:

[0053] The trapping effects of different ratios of attractants and oviposition attractants on pear fruit borer in the field.

[0054] Egg-laying attractants and sex attractants were prepared into mixed solutions with dosage ratios of 100:1, 10:1, 1:1, 1:10, and 1:100 as treatments. Single egg-laying attractants, single sex attractants, hexane as solvent, and empty lures (with rubber stoppers) served as controls. A checkerboard sampling method was used to suspend the lures and triangular traps at intervals of approximately 30 m and a suspension height of approximately 1.5 m. The sticky insect sheets were collected and replaced every 3 days in the morning, and the trapping count was recorded in the laboratory.

[0055] like Figure 3 As shown, there were significant differences in the trapping rates of pear fruit moth in the field among different ratios of sex pheromone and oviposition pheromone attractants (F=6.287, df=12.64, P<0.05). Specifically, the trapping rates of pear fruit moth in the field were significantly higher at 10 and 100 μL of sex pheromone, and at oviposition pheromone to sex pheromone ratios of 10:1 and 100:1 than at empty lures and the hexane control.

[0056] Furthermore, when the ratio of oviposition attractant to sex attractant was 10:1 and 100:1, the trapping effect on pear fruit moth in the field was higher than that of 10 and 100 μL of sex attractant alone.

[0057] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details.

Claims

1. An active ingredient for a pear fruit borer trapping agent, characterized by, include: Sex attractants, including cis-8-dodecenoyl acetate, trans-8-dodecenoyl acetate, cis-8-dodecenoyl alcohol, and dodecano-1-ol; And oviposition attractants, including benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol; in; The volume ratio of the sex attractant to the oviposition attractant is 10:1; In the aforementioned sex attractant, the mass ratio of cis-8-dodecenoyl acetate, trans-8-dodecenoyl acetate, cis-8-dodecenoyl alcohol, and dodecyl-1-ol is 100:6.8:19.1:5.

4. In the oviposition attractant, the mass ratio of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl butyrate, and cis-3-hexenol is 100:1:100:1000:10 or 100:1000: 10：1：100。 2. A codling moth trapping agent, characterized by, Includes the active ingredient of the pear fruit moth trap and n-hexane; in, The active components of the pear fruit moth trap include a sex attractant and an oviposition attractant in a volume ratio of 10:

1. The sex attractant includes cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecenol-1-ol; the oviposition attractant includes benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol. In the aforementioned sex attractant, the mass ratio of cis-8-dodecenoyl acetate, trans-8-dodecenoyl acetate, cis-8-dodecenoyl alcohol, and dodecyl-1-ol is 100:6.8:19.1:5.

4. In the oviposition attractant, the mass ratio of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl butyrate, and cis-3-hexenol is 100:1:100:1000:10 or 100:1000:10:1:

100.

3. A method of trapping the codling moth, Carpocapsa pomonella, characterized in that, Includes the following steps: The pear fruit moth trap was prepared by adding the active components of the trap to n-hexane. The active components of the pear fruit moth trap included a sex attractant and an oviposition attractant in a volume ratio of 10:

1. The sex attractant included cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecenol-1-ol. The oviposition attractant included benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenylbutyrate, and cis-3-hexenol. A lure core is prepared by adding a pear fruit moth trap into a centrifuge tube with a rubber stopper or a polypropylene plastic centrifuge tube. The lure is placed on the trap and suspended on the pear tree for trapping. The trap is suspended at a height of 1.5m and at intervals of >30m. in; The mass ratio of cis-8-dodecenol acetate, trans-8-dodecenol acetate, cis-8-dodecenol, and dodecyl-1-ol is 100:6.8:19.1:5.

4. The mass ratio of benzaldehyde, benzonitrile, trans-2-hexenal, cis-3-hexenyl butyrate, and cis-3-hexenol is 100:1:100:1000:10 or 100:1000:10:1:

100. The concentration of the sex attractant is 13.1 μg / μL, and the concentration of the oviposition attractant is 5 μg / μL.

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