A compound medicament for treating trichomonas gallinae, a preparation method thereof and a medicine

Abstract

The application discloses a compound medicament for treating trichomonas gallinae, a preparation method of the compound medicament and a medicine, and aims to provide a novel, rapid and safe treatment formula which can replace metronidazole in treating trichomonas gallinae disease. The compound medicament prepared by mixing the aqueous extract of Sophora flavescens and the copper-containing adjuvant can kill the trichomonas gallinae in the pigeon in a short time, and cannot cause drug resistance and drug residue problems, and the formula of the application is simpler and has a shorter medication time than other traditional Chinese medicine formulas.

Description

A compound drug for treating trichomoniasis in pigeons, its preparation method, and the drug itself. Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a compound medicine for treating trichomoniasis in pigeons, its preparation method, and the medicine itself. Background Technology

[0002] Trichomoniasis, also known as oral ulcers or "pigeon scabies," is a common pigeon disease caused by Trichomonas vaginalis. The main route of infection is oral, where large numbers of parasites accumulate in the oral ulcer lesions and saliva. Adult pigeons are directly infected through kissing or feeding young pigeons with crop milk, and can also be infected through drinking water and wounds. Approximately 80% of pigeons are carriers. Adult pigeons show few symptoms, while young pigeons exhibit prominent symptoms. Young pigeons are usually infected upon first consuming pigeon milk from the crop of a young pigeon and remain carriers for life. Before developing sufficient immunity, the mortality rate after infection with highly virulent strains can reach 50%. Trichomoniasis in pigeons is not localized to one location. Therefore, depending on the location of the parasite damage, pigeon trichomoniasis is classified into three types: pharyngeal, visceral, and umbilical. (1) Pharyngeal type: This is the most common and most harmful type. Affected pigeons often experience difficulty swallowing and breathing due to oral damage. They become depressed, have disordered digestion, ruffled feathers, become emaciated, have reduced appetite and increased thirst, and excrete yellow-green watery feces. Young pigeons often present with acute symptoms and die from respiratory distress within a short period of time. (2) Visceral type: This is a further development of other types of disease. Affected pigeons are lethargic, have poor appetite, ruffled feathers, increased water intake, yellow-green mucous diarrhea, progressive emaciation, and collapse. If the respiratory tract is damaged, the affected pigeon will have symptoms such as open mouth or neck stretching posture, coughing, and panting when breathing. If the intestines are infected (intestinal type), the affected pigeon will refuse to eat, have ruffled feathers, tremble, and excrete pale yellow pasty feces. They are very likely to become emaciated or even die quickly. (3) Umbilical type: This is caused by infection of the navel of young pigeons. Affected pigeons are lethargic, have a poor appetite, ruffled feathers, become emaciated, stand listlessly to one side, and have redness and inflammation of the navel area, and are unwilling to lie down. At present, the treatment of trichomoniasis in pigeons still widely uses banned drugs such as metronidazole, which not only causes a large number of trichomoniasis to develop metronidazole resistance, making it impossible to cure, but also drug residues will cause huge food safety risks.

[0003] Sophora flavescens (scientific name: *Sophora flavescens*) is a common herbaceous plant belonging to the genus *Sophora* in the legume family. Also known as Huangtengmu or Huanglancao, it is widely distributed in many parts of Asia, including China, Japan, the Korean Peninsula, and Southeast Asia. Sophora flavescens is widely used in Traditional Chinese Medicine (TCM). Its roots and bark are rich in bioactive chemical components with various medicinal values. The most important components are flavonoids, including matrine and matrine, which possess anti-inflammatory, antioxidant, antibacterial, and antitumor activities. In TCM, Sophora flavescens is used for clearing heat and detoxifying, removing blood stasis and relieving pain, reducing swelling and relieving itching. It is commonly used to treat skin conditions such as eczema, acne, pruritus, and prickly heat. Furthermore, it is also used to treat hepatitis, nephritis, and ulcerative colitis. Phellodendron amurense, commonly known as Huangbai, is a common traditional Chinese medicine. Belonging to the Zanthoxylum family, it is a deciduous tree primarily found in Northeast China and the Russian Far East, but also widely cultivated elsewhere, including Japan and the Korean Peninsula. The bark, typically harvested in autumn, is the main medicinal part. It is yellowish-brown and rich in flavonoids, alkaloids, and bitter components. The bark also contains various active ingredients such as mandelic acid, flavonoid glycosides, and alkaloids. Widely used in traditional Chinese medicine, Huangbai possesses diverse pharmacological activities and medicinal value, serving as a medicine for clearing heat and detoxifying, reducing inflammation, antibacterial, antioxidant, and anticancer purposes. It can treat symptoms such as colds, fever, and oral ulcers, and is used in traditional Chinese medicine to treat hepatitis, gastritis, and ulcerative colitis. Furthermore, Huangbai has certain antibacterial properties, inhibiting various bacteria and fungi, and is used in the preparation of antibacterial, antifungal, and antiviral agents. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a compound medicine for treating Trichomonas vaginalis, its preparation method and the medicine, so as to solve the technical problems of poor efficacy and easy development of drug resistance in the treatment of Trichomonas vaginalis by existing medicines.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is: a method for preparing a compound medicine for treating trichomoniasis in pigeons, comprising the following steps:

[0006] S1: Mix Sophora flavescens powder, Phellodendron chinense powder and water in a ratio of 100-200g: 100-200g: 2000-3000mL and soak for 1.5-2.5h. Then heat at 1800-2200W for 8-12min, then adjust the power to 200-400W and heat for 25-35min. Filter to obtain liquid A.

[0007] S2: Add 2000-3000mL of water to the filter residue, mix well, and heat at 1800-2200W for 8-12 minutes. Then adjust the power to 200-400W and heat for 25-35 minutes. Filter to obtain drug solution B.

[0008] S3: Mix drug solution A and drug solution B, centrifuge at 2800-3200 rpm for 8-12 min, take the supernatant and heat at 800-1000W for 20-40 min to obtain the water extract of Sophora flavescens and Phellodendron chinense.

[0009] S4: Mix the water extracts of Sophora flavescens and Phellodendron chinense with a copper-containing adjuvant to obtain a compound medicine for treating Trichomonas vaginalis in pigeons.

[0010] Based on the above technical solution, the present invention can be further improved as follows:

[0011] Furthermore, the particle size of Sophora flavescens powder and Phellodendron amurense powder is 0.2-0.4 mm.

[0012] Furthermore, the concentration of the aqueous extract of Sophora flavescens and Phellodendron chinense is 200-500 mg / mL.

[0013] Furthermore, the concentration of the aqueous extract of Sophora flavescens and Phellodendron chinense was 300 mg / mL.

[0014] Furthermore, the copper adjuvant is a copper sulfate solution of 0.05-1 wt%.

[0015] Furthermore, the copper adjuvant is a 0.1 wt% copper sulfate solution.

[0016] Furthermore, the volume ratio of the water extract of Sophora flavescens and Phellodendron amurense to the copper-containing adjuvant is 50-65:1.

[0017] The present invention also discloses a drug comprising a compound preparation for treating trichomoniasis in pigeons.

[0018] The present invention also discloses the application of a drug for treating trichomoniasis in pigeons.

[0019] Furthermore, the drug is administered orally.

[0020] The beneficial effects of this invention are as follows: This invention mixes the water extract of Sophora flavescens and Phellodendron chinense with copper sulfate, which can quickly, safely and effectively kill Trichomonas vaginalis and treat Trichomonas vaginalis disease compared with other drugs. The compound medicine prepared by this invention is simple and convenient to use and will not produce drug resistance. Attached Figure Description

[0021] Figure 1 is a comparison of the effects of the compound preparation obtained in this invention and metronidazole.

[0022] Figure 2 shows the time required for the compound drug prepared in this invention to kill parasites in diseased pigeons after administration. Detailed Implementation

[0023] The specific embodiments of the present invention are described below to facilitate understanding of the invention by those skilled in the art. Unless otherwise specified, specific conditions are applied according to conventional conditions or the manufacturer's recommendations. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products. However, it should be understood that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, various modifications are obvious as long as they fall within the spirit and scope of the invention as defined and determined by the appended claims. All inventions utilizing the concept of this invention are protected.

[0024] Example 1:

[0025] A compound medicine for treating trichomoniasis in pigeons, the preparation method of which includes the following steps:

[0026] S1: Mix Sophora flavescens powder, Phellodendron chinense powder and water with a particle size of 0.3mm in a ratio of 100g:100g:2000mL and soak for 2 hours. Then heat at 2000W for 10 minutes, then adjust the power to 300W and heat for 30 minutes. Filter to obtain liquid A.

[0027] S2: Add 3000mL of water to the filter residue, mix well, and heat at 2000W for 10 minutes. Then adjust the power to 300W and heat for 30 minutes. Filter to obtain drug solution B.

[0028] S3: Mix drug solution A and drug solution B, centrifuge at 3000 rpm for 10 min, take the supernatant and heat at 900 W for 30 min to obtain a water extract of Sophora flavescens and Phellodendron chinense with a concentration of 300 mg / mL.

[0029] S4: Mix the water extract of Sophora flavescens and Phellodendron chinense with a copper sulfate solution of 0.1 wt% at a volume ratio of 60:1 to obtain a compound medicine for treating Trichomonas vaginalis in pigeons.

[0030] Example 2:

[0031] A compound medicine for treating trichomoniasis in pigeons, the preparation method of which includes the following steps:

[0032] S1: Mix Sophora flavescens powder, Phellodendron chinense powder and water with a particle size of 0.2 mm in a ratio of 100g:200g:3000mL and soak for 1.5h. Then heat at 1800W for 12min, then adjust the power to 400W and heat for 25min. Filter to obtain liquid A.

[0033] S2: Add 2000mL of water to the filter residue, mix well, and heat at 2200W for 8 minutes. Then adjust the power to 200W and heat for 35 minutes. Filter to obtain drug solution B.

[0034] S3: Mix drug solution A and drug solution B, centrifuge at 2800 rpm for 12 min, take the supernatant and heat at 800 W for 40 min to obtain a water extract of Sophora flavescens and Phellodendron chinense with a concentration of 500 mg / mL.

[0035] S4: Mix the water extract of Sophora flavescens and Phellodendron chinense with a copper sulfate solution of 0.05 wt% at a volume ratio of 50:1 to obtain a compound medicine for treating Trichomonas vaginalis in pigeons.

[0036] Example 3:

[0037] A compound medicine for treating trichomoniasis in pigeons, the preparation method of which includes the following steps:

[0038] S1: Mix Sophora flavescens powder, Phellodendron chinense powder and water with a particle size of 0.4 mm in a ratio of 200g:100g:3000mL and soak for 2.5h. Then heat at 2200W for 8min, then adjust the power to 200W and heat for 35min. Filter to obtain liquid A.

[0039] S2: Add 2000mL of water to the filter residue, mix well, and heat at 1800W for 12 minutes. Then adjust the power to 400W and heat for 25 minutes. Filter to obtain drug solution B.

[0040] S3: Mix drug solution A and drug solution B, centrifuge at 3200 rpm for 8 min, take the supernatant and heat at 1000 W for 20 min to obtain a water extract of Sophora flavescens and Phellodendron chinense with a concentration of 200 mg / mL.

[0041] S4: Mix the water extract of Sophora flavescens and Phellodendron chinense with a 1 wt% copper sulfate solution at a volume ratio of 65:1 to obtain a compound medicine for treating Trichomonas vaginalis in pigeons.

[0042] Comparative example:

[0043] A water extract of Sophora flavescens and Phellodendron chinense, the preparation method of which includes the following steps:

[0044] S1: Mix Sophora flavescens powder, Phellodendron chinense powder and water with a particle size of 0.3mm in a ratio of 100g:100g:2000mL and soak for 2 hours. Then heat at 2000W for 10 minutes, then adjust the power to 300W and heat for 30 minutes. Filter to obtain liquid A.

[0045] S2: Add 3000mL of water to the filter residue, mix well, and heat at 2000W for 10 minutes. Then adjust the power to 300W and heat for 30 minutes. Filter to obtain drug solution B.

[0046] S4: Mix drug solution A and drug solution B, centrifuge at 3000 rpm for 10 min, take the supernatant and heat at 900 W for 30 min to obtain a water extract of Sophora flavescens and Phellodendron chinense with a concentration of 300 mg / mL.

[0047] Experimental example:

[0048] The test subjects were white-feathered king pigeons, weighing 400-700g each. The laboratory temperature was 20-22℃, with ventilation fans and natural light for 12 hours per day. They were caged, with one pair of white pigeons (one male and one female) per cage. The cages were cleaned every three days.

[0049] 1. Effects on isolated pigeon trichomonads

[0050] Trichomonas vaginalis was collected from the upper respiratory tract of diseased pigeons using a swab. The collected samples were then transferred to a medium consisting of 4.5 mL TYM basal medium, 0.5 mL fetal bovine serum, 25 μL penicillin, 25 μL streptomycin, and 5 μL chloramphenicol. The medium was incubated at 37°C, with daily changes. The pigeons were then passaged to the logarithmic growth phase, and the concentration of Trichomonas vaginalis was diluted to 1 × 10⁻⁶. 6 The number of cells / mL was divided into four equal portions for the experiment. The same volume of 300 mg / mL Sophora flavescens and Phellodendron amurense aqueous extract (comparative example), 0.1 wt% copper sulfate, and a mixture of 300 mg / mL Sophora flavescens and Phellodendron amurense aqueous extract + 0.1 wt% copper sulfate (the compound preparation obtained in Example 1) were added to the *Trichomonas peltatum* solution to administer the isolated *Trichomonas peltatum*. The positive control group was 100 μM metronidazole. The activity of *Trichomonas peltatum* was continuously observed under an optical microscope. The experimental results are shown in Figure 1.

[0051] Experimental results showed that metronidazole required 12 hours to completely kill Trichomonas vaginalis (reducing the number of Trichomonas vaginalis by 100%). The complete killing time required by 300 mg / mL Sophora flavescens and Phellodendron amurense aqueous extract (SACC), 0.1 wt% copper sulfate (CS), and the mixture of 300 mg / mL SACC and 0.1 wt% CS (the compound preparation prepared in Example 1) was shorter than that of metronidazole. The time required for 300 mg / mL SACC was 6 hours, the time required for 0.1% CS was 2 hours, and the time required for the compound preparation was only 1 hour. This indicates that the mixture of Sophora flavescens and Phellodendron amurense aqueous extract and copper sulfate has a significant killing effect on isolated Trichomonas vaginalis.

[0052] 2. Effects on in vivo pigeon trichomonas

[0053] Trichomonas vaginalis was collected from the upper respiratory tract of diseased pigeons using swabs soaked in PBS. The collected pigeon trichomoniasis were counted using a live cell counter, and the initial number of pigeon trichomoniasis was found to be 3.47 × 10⁻⁶. 6 The concentration of Trichomonas vaginalis was measured per mL. Then, 100 mL of the compound preparation from Example 1 was administered to pigeons via free drinking water. Every 24 hours, swabs soaked in PBS were used to collect Trichomonas vaginalis from the upper respiratory tract of the sick pigeons. The samples were examined under an optical microscope and counted using a live cell counter. The experimental results are shown in Figure 2.

[0054] The results showed that, 24 hours after administration of the compound agent prepared in Example 1, no Trichomonas vaginalis was detected in the diseased pigeons, indicating that the Trichomonas vaginalis in the pigeons had been completely killed; 48 hours after administration, no Trichomonas vaginalis was detected in the diseased pigeons, indicating that the Trichomonas vaginalis did not recur after being killed and there was no drug resistance. This shows that the compound agent prepared in this invention can rapidly, significantly and effectively treat trichomoniasis in pigeons.

Claims

1. A method for preparing a compound medicine for treating trichomoniasis in pigeons, characterized in that, Includes the following steps: S1: Mix Sophora flavescens powder, Phellodendron amurense powder, and water in a ratio of 100-200g:100-200g:2000-3000mL and soak for 1.5-2.5 hours. Then heat at 1800-2200W for 8-12 minutes, then adjust the power to 200-400W and heat for 25-35 minutes. Filter to obtain solution A. S2: Add 2000-3000mL of water to the filter residue, mix well, and heat at 1800-2200W for 8-12 minutes. Then adjust the power to 200-400W. S3: Mix the drug solutions A and B, centrifuge at 2800-3200 rpm for 8-12 minutes, take the supernatant and heat at 800-1000W for 20-40 minutes to obtain a 200-500 mg / mL aqueous extract of Sophora flavescens and Phellodendron chinense; S4: Mix the aqueous extract of Sophora flavescens and Phellodendron chinense with a copper adjuvant at a volume ratio of 50-65:1 to obtain the compound medicine for treating Trichomonas vaginalis in pigeons; the copper adjuvant is a 0.05-1 wt% copper sulfate solution.

2. The method for preparing the compound medicine for treating trichomoniasis in pigeons according to claim 1, characterized in that: The particle size of the sophora flavescens powder and phellodendron amurense powder is 0.2-0.4 mm.

3. The method for preparing the compound medicine for treating trichomoniasis in pigeons according to claim 1, characterized in that: The concentration of the aqueous extract of Sophora flavescens and Phellodendron chinense was 300 mg / mL.

4. The method for preparing the compound medicine for treating trichomoniasis in pigeons according to claim 1, characterized in that: The copper-containing adjuvant is a 0.1 wt% copper sulfate solution.

5. A compound preparation for treating trichomoniasis of pigeons prepared by the method according to any one of claims 1-4.

6. The compound medicine for treating trichomoniasis in pigeons according to claim 5, characterized in that: The compound medicine is administered orally.

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