Amino-functionalized gold nanoparticle ink modified ternary heterojunction biosensor and preparation method thereof

Abstract

This disclosure relates to a ternary heterojunction biosensor modified with aminated gold nano-ink and its preparation method, specifically involving the field of photoanode thin films for biosensors. The biosensor comprises a conductive substrate, and sequentially disposed on one side of the conductive substrate a TiO2 / BiVO4 / MoS2 composite thin film layer, an aminated gold nano-ink layer, and an aptamer probe layer. The preparation method includes: 1. Obtaining BiVO4 powder, a TiO2 / BiVO4 / MoS2 composite, aminated gold nano-ink, and an aptamer probe solution; 2. Printing the TiO2 / BiVO4 / MoS2 composite onto the conductive substrate to form a TiO2 / BiVO4 / MoS2 composite thin film layer; 3. Printing the aminated gold nano-ink onto the TiO2 / BiVO4 / MoS2 composite thin film layer to obtain a photoanode thin film; 4. Spraying the aptamer probe solution onto the photoanode thin film in a predetermined amount, and sealing non-specific binding sites with a protein-free blocking solution.

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