Dendrobium wilt disease prevention and treatment biological agent and preparation method thereof
By preparing a Dendrobium twig blight control agent containing garlic fermentation extract, garlic extract, methylcyclopentylsiloxane, and methyl thiophanate, the problem of Dendrobium twig blight control has been solved, achieving effective protection of Dendrobium plants and improving economic benefits.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ANHUI YIPIN VILLA MODERN AGRI DEV CO LTD
- Filing Date
- 2023-10-11
- Publication Date
- 2026-06-19
AI Technical Summary
Current technology lacks effective agents to control Dendrobium twig blight, making Dendrobium plants susceptible to pathogen infection and affecting yield and quality.
A biological agent with garlic fermentation extract, garlic extract, methylcyclopentasiloxane and thiophanate-methyl as the main components was prepared by using starch semi-gelatinization and high-pressure gas explosion treatment, combined with the slow-release effect of methylcyclopentasiloxane and thiophanate-methyl, to control Dendrobium twig blight.
It effectively prevents and controls Dendrobium twig blight, inhibits pathogens, reduces plant infection, improves planting economic benefits, and prolongs efficacy and reduces the frequency of use through starch modification treatment.
Smart Images
Figure BDA0004486873030000071
Abstract
Description
Technical Field
[0001] This invention relates to the field of Dendrobium cultivation technology, specifically to a biological agent for the prevention and control of Dendrobium shoot blight and its preparation method. Background Technology
[0002] Dendrobium is a precious plant that can be used both as food and medicine. The main medicinal part is its fresh or dried stem, which has both health-promoting and therapeutic effects. Dendrobium officinale has a long history of medicinal use and high medicinal value, often referred to as "the gold standard of medicine." It helps to promote the production of body fluids and nourish the stomach; clear heat and nourish yin; benefit the kidneys and lungs; and strengthen the waist and improve eyesight. Due to its high nutritional and medicinal value, Dendrobium has evolved from being harvested wild to being cultivated artificially.
[0003] Dendrobium twig blight is a common disease in Dendrobium cultivation, generally caused by *Phytophthora hymex*, a species of fungus belonging to the genus *Phytophthora* in the family Pythaceae. Symptoms primarily involve yellowing and wilting of the tender shoot tips and young leaves. In severe cases, the disease can spread to the stem. Dendrobium can be infected at both the seedling and mature stages. Furthermore, because the pathogen infects the base of the stem, it can cause root rot in transplanted seedlings and damage to the entire plant.
[0004] Symptoms: This disease mainly affects the tender leaves at the tips of shoots, and in severe cases, it can also damage the stems. Both seedlings and mature plants can be infected. When the pathogen infects the base of the stem, it can cause root rot, wilting, and death in transplanted seedlings of the same year. It can also infect the tender stems of 2-3 year old plants, causing only top dieback. Mature plants are particularly susceptible to the disease during the hot and humid summer months, which seriously affects the yield and quality of Dendrobium. Current treatment methods generally involve promptly removing diseased seedlings and disinfecting them. There are currently no specific control agents for this disease. Summary of the Invention
[0005] To address the shortcomings of existing technologies, this invention provides a biological agent for the prevention and control of Dendrobium twig blight and its preparation method, which effectively prevents and controls Dendrobium twig blight, reduces pathogen infection of plants, ensures plant health, and improves the economic benefits of cultivation.
[0006] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solution:
[0007] A biological agent for controlling Dendrobium twig blight, the biological agent being made from the following raw materials in parts by weight: 10-16 parts starch, 2-4 parts garlic fermentation extract, 1-2 parts garlic extract, 1-3 parts methylcyclopentasiloxane, and 2-6 parts methylthiophanate; wherein the garlic fermentation extract is obtained by fermenting garlic with a mixture of Bifidobacterium and Lactobacillus casei; and the garlic extract is obtained by grinding and pulverizing garlic and then extracting it with ethanol and ultrasound.
[0008] Preferably, the specific preparation method of the garlic fermentation extract includes the following steps:
[0009] S1-1. Take fresh garlic, add 3 times the volume of deionized water, grind into a paste, and then keep warm in a water bath for 2-4 hours to obtain pre-treated garlic paste for later use.
[0010] S1-2. Add glucose to the pretreated garlic slurry and stir well. Then add Bifidobacterium and Lactobacillus casei. Ferment for 4-6 days, press and filter, sterilize the filtrate under high pressure and freeze dry to obtain garlic fermentation extract.
[0011] Preferably, the temperature of the water bath heat preservation treatment in step S1-1 is 55-65℃.
[0012] Preferably, in steps S1-2, the amounts of glucose, bifidobacteria, and lactobacillus added are 4%, 0.5%, and 0.2% of the mass of the pretreated garlic slurry, respectively.
[0013] Preferably, the method for preparing the garlic extract includes the following steps:
[0014] S2-1. Take fresh garlic, add 5 times its volume of 35% ethanol solution and grind it into a paste to obtain a pre-made paste for later use.
[0015] S2-2. The above-mentioned pre-made slurry is kept warm in a water bath and then ultrasonically treated. After filtration, the filter residue is subjected to the above operation and filtered again. The two filtrates are combined and freeze-dried to obtain garlic extract.
[0016] Preferably, in step S2-2, the temperature of the water bath ultrasonic treatment is 45-55℃, the power is 500W, and the treatment time is 15min.
[0017] The preparation method of the biological agent for controlling Dendrobium twig blight includes the following steps:
[0018] (1) Starch pretreatment: The starch raw material is added to twice the amount of water and semi-gelatinized at 65°C, then dried and ground, and then subjected to high-pressure gas explosion treatment to obtain pretreated starch for use.
[0019] (2) Add the above pretreated starch to 5 times the amount of water, heat and stir evenly, then add garlic fermentation extract and garlic extract, and continue stirring until the solution becomes a paste, and the mixture is ready for use.
[0020] (3) Cool the above mixture to room temperature, then add methylcyclopentanol and methyl thiophanate, continue to stir evenly, freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0021] Preferably, in step (1), the pressure of the high-pressure gas explosion is 2 MPa and the gas explosion time is 45-55 s.
[0022] Preferably, the temperature for heating and stirring in step (2) is 60-70℃, and the stirring speed is 120-140r / min.
[0023] Preferably, in step (3), the stirring speed is 80-100 r / min and the stirring time is 10-15 min.
[0024] This invention provides a biological agent for controlling Dendrobium twig blight and its preparation method, which has the following advantages compared with the prior art:
[0025] (1) This invention uses garlic fermentation extract, garlic extract, methylcyclopentasiloxane and methyl thiophanate as effective bactericidal components, which can effectively prevent and control Dendrobium twig blight and effectively inhibit pathogens. While preventing Dendrobium from developing diseases, it can also have a certain therapeutic effect on Dendrobium that has already developed diseases, thereby improving the overall economic benefits of planting.
[0026] (2) The present invention modifies starch by semi-gelatinization and steam explosion and then mixes it with various sterilization components. This can effectively adsorb the sterilization components and achieve a certain slow release effect, prolong the action time of the drug on the crop, reduce the frequency of drug use, and improve the convenience of planting. Detailed Implementation
[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0028] Example 1:
[0029] Raw material preparation:
[0030] 1. Garlic fermentation extract:
[0031] (1) Take fresh garlic, add 3 times the volume of deionized water and grind it into a paste. Then, keep it in a water bath at 60°C for 3 hours to obtain pre-treated garlic paste for later use.
[0032] (2) Add 4% glucose by weight of the pretreated garlic slurry and stir well. Then add 0.5% Bifidobacterium and 0.2% Lactobacillus casei by weight of the slurry. Mix and ferment at 35°C for 5 days, then press and filter. Sterilize the filtrate at 121°C (103.4 kPa steam pressure) for 30 minutes, then freeze dry to obtain garlic fermentation extract.
[0033] 2. Preparation of garlic extract:
[0034] (1) Take fresh garlic, add 5 times the volume of 35% ethanol solution and grind it into a paste to obtain a pre-made paste for later use;
[0035] (2) The above pre-made slurry was kept in a water bath at 50°C and then ultrasonically treated with 500W power for 15 minutes. After filtration, the filter residue was subjected to the above operation and then filtered again. The two filtrates were combined and freeze-dried to obtain garlic extract.
[0036] Example 2:
[0037] Preparation of biological agents for the control of Dendrobium twig blight:
[0038] (1) Prepare the raw materials according to the following weight parts: 10 parts starch, 2 parts garlic fermentation extract, 1 part garlic extract, 1 part methylcyclopentanolsiloxane, and 2 parts methyl thiophanate.
[0039] (2) Add twice the amount of water to the starch raw material and semi-gelatinize it at 65°C. Then dry and grind it. Finally, use 2 MPa pressure steam explosion treatment for 50 seconds to obtain pretreated starch for later use.
[0040] (3) Add the above pretreated starch to 5 times the amount of water and heat to 65°C. After stirring evenly, add garlic fermentation extract and garlic extract. Stir at 120 r / min until the solution becomes a paste and the mixture is ready for use.
[0041] (4) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0042] Example 3:
[0043] Preparation of biological agents for the control of Dendrobium twig blight:
[0044] (1) Prepare the raw materials according to the following weight parts: 16 parts starch, 4 parts garlic fermentation extract, 2 parts garlic extract, 3 parts methylcyclopentanolsiloxane, and 6 parts methyl thiophanate.
[0045] (2) Add twice the amount of water to the starch raw material and semi-gelatinize it at 65°C. Then dry and grind it. Finally, use 2 MPa pressure steam explosion treatment for 50 seconds to obtain pretreated starch for later use.
[0046] (3) Add the above pretreated starch to 5 times the amount of water and heat to 65°C. After stirring evenly, add garlic fermentation extract and garlic extract. Stir at 120 r / min until the solution becomes a paste and the mixture is ready for use.
[0047] (4) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0048] Comparative Example 1:
[0049] Preparation of biological agents for the control of Dendrobium twig blight:
[0050] (1) Prepare the raw materials according to the following weight parts: 16 parts starch, 6 parts garlic extract, 3 parts methylcyclopentanolsiloxane, and 6 parts methyl thiophanate.
[0051] (2) Add twice the amount of water to the starch raw material and semi-gelatinize it at 65°C. Then dry and grind it. Finally, use 2 MPa pressure steam explosion treatment for 50 seconds to obtain pretreated starch for later use.
[0052] (3) Add the above pretreated starch to 5 times the amount of water and heat to 65°C. After stirring evenly, add garlic extract and stir at 120 r / min until the solution becomes a paste. The mixture is then ready for use.
[0053] (4) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0054] Comparative Example 2:
[0055] Preparation of biological agents for the control of Dendrobium twig blight:
[0056] (1) Prepare the raw materials according to the following parts by weight: 16 parts starch, 6 parts garlic fermentation extract, 3 parts methylcyclopentanolsiloxane, and 6 parts methyl thiophanate.
[0057] (2) Add twice the amount of water to the starch raw material and semi-gelatinize it at 65°C. Then dry and grind it. Finally, use 2 MPa pressure steam explosion treatment for 50 seconds to obtain pretreated starch for later use.
[0058] (3) Add the above pretreated starch to 5 times the amount of water and heat to 65°C. After stirring evenly, add garlic fermentation extract and stir at 120 r / min until the solution becomes a paste. The mixture is then ready for use.
[0059] (4) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0060] Comparative Example 3:
[0061] Preparation of biological agents for the control of Dendrobium twig blight:
[0062] (1) Prepare the raw materials according to the following weight parts: 16 parts starch, 3 parts methylcyclopentanolsiloxane, and 6 parts methyl thiophanate.
[0063] (2) Add twice the amount of water to the starch raw material and semi-gelatinize it at 65°C. Then dry and grind it. Finally, use 2 MPa pressure steam explosion treatment for 50 seconds to obtain pretreated starch for later use.
[0064] (3) Add the pretreated starch to 5 times the amount of water and heat to 65°C. Stir at 120 r / min until the solution becomes a paste and the mixture is ready for use.
[0065] (4) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0066] Comparative Example 4:
[0067] Preparation of biological agents for the control of Dendrobium twig blight:
[0068] (1) Prepare the raw materials according to the following weight parts: 16 parts starch, 4 parts garlic fermentation extract, 2 parts garlic extract, 3 parts methylcyclopentanolsiloxane, and 6 parts methyl thiophanate.
[0069] (2) Add starch to 5 times the amount of water and heat to 65°C. Stir well and then add garlic fermentation extract and garlic extract. Stir at 120 r / min until the solution becomes a paste. The mixture is ready for use.
[0070] (3) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue stirring at 100 r / min for 12 min, then freeze dry and pulverize to obtain a biological agent for the control of Dendrobium twig blight.
[0071] Detection:
[0072] 1. Pathogen extraction:
[0073] Diseased tissues of Dendrobium officinale grown in the field were collected and wiped with cotton balls soaked in 75% alcohol. The terminal leaves of the diseased tissue were evenly cut into small pieces of 1.0cm×1.0cm. The tissues were soaked in 75% alcohol solution for 30 seconds, then disinfected with 1% NaClO for 4 minutes, and then rinsed with 75% alcohol for 30 seconds to remove residual NaClO. After rinsing three times with sterile water, the tissues were dried and placed on potato dextrose agar medium. The medium was incubated at 28℃ for 7 days until a single colony grew on the plate. This colony was identified as the pathogen of Dendrobium officinale twig blight.
[0074] 2. The biological agents for controlling Dendrobium twig blight prepared in Examples 2-3 and Comparative Examples 1-4 were selected and diluted 200 times with water. Then, each agent was sprayed onto the culture medium containing the pathogen at 5% of the culture medium volume. The mixtures were then incubated at 28℃ for 3, 5, and 10 days. The sterilization rate of each group was calculated, and the specific results are shown in Table 1 below.
[0075] Table 1
[0076]
[0077] As shown in the table above, the products prepared in Examples 2-3 can effectively inhibit and kill pathogens.
[0078] 3. The above-mentioned pathogens were inoculated onto the tips of healthy Dendrobium leaves. Then, the Dendrobium twig blight control biological agents prepared in Examples 2-3 and Comparative Examples 1-4 were diluted 200 times with water and sprayed onto the leaf tips. After 14 days of continued planting, the presence of lesions on the leaf tips was recorded, along with the number of lesions (30 plants per group). The specific results are shown in Table 2 below.
[0079] Table 2
[0080] Group Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Number of lesions 0 0 2 4 11 0
[0081] As shown in the table above, the agents prepared in Examples 2-3 can effectively prevent and control the twig blight of Dendrobium and can play a long-lasting sterilization role.
[0082] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A biological agent for controlling Dendrobium twig blight, characterized in that, The biological agent is made from the following raw materials in parts by weight: 10-16 parts starch, 2-4 parts garlic fermentation extract, 1-2 parts garlic extract, 1-3 parts methylcyclopentanol siloxane, and 2-6 parts methyl thiophanate. The garlic fermentation extract mentioned above is prepared by fermenting garlic with a mixture of Bifidobacterium and Lactobacillus casei. The specific preparation method of garlic fermentation extract includes the following steps: S1-1. Take fresh garlic, add 3 times the volume of deionized water, grind into a paste, and then keep warm in a water bath for 2-4 hours to obtain pre-treated garlic paste for later use. S1-2. Add glucose to the above pretreated garlic slurry and stir well. Then add Bifidobacterium and Lactobacillus casei. Ferment for 4-6 days, press and filter, sterilize the filtrate under high pressure and freeze dry to obtain garlic fermentation extract. The garlic extract was prepared by grinding garlic into powder and then extracting it with a combination of ethanol and ultrasound. The preparation method of garlic extract includes the following steps: S2-1. Take fresh garlic, add 5 times its volume of 35% ethanol solution and grind it into a paste to obtain a pre-made paste for later use. S2-2. The above-mentioned pre-made slurry is kept warm in a water bath and then ultrasonically treated, then filtered. The filter residue is subjected to the above operation again and filtered again. The two filtrates are combined and freeze-dried to obtain garlic extract. The preparation method of the biological agent for controlling Dendrobium twig blight includes the following steps: (1) Starch pretreatment: The starch raw material is added to twice the amount of water and semi-gelatinized at 65°C, then dried and ground, and then subjected to high-pressure gas explosion treatment to obtain pretreated starch for use. (2) Add the above pretreated starch to 5 times the amount of water, heat and stir evenly, then add garlic fermentation extract and garlic extract, and continue stirring until the solution becomes a paste, and the mixture is ready for use. (3) Cool the above mixture to room temperature, then add methylcyclopentasiloxane and methylthiophanate, continue to stir evenly, freeze dry and pulverize to obtain a biological agent for the prevention and control of Dendrobium twig blight.
2. The biological agent for controlling Dendrobium twig blight according to claim 1, characterized in that: The temperature of the water bath insulation treatment in step S1-1 is 55-65℃.
3. The biological agent for preventing and treating Dendrobium wilt according to claim 1, characterized in that: In steps S1-2, the amounts of glucose, bifidobacteria, and lactobacillus added are 4%, 0.5%, and 0.2% of the mass of the pretreated garlic slurry, respectively.
4. The biological agent for controlling Dendrobium twig blight according to claim 1, characterized in that, In step S2-2, the water bath ultrasonic treatment is performed at a temperature of 45-55℃, a power of 500W, and a treatment time of 15 minutes.
5. The biological agent for preventing and treating Dendrobium wilt according to claim 1, characterized in that: In step (1), the pressure of the high-pressure gas explosion is 2 MPa and the gas explosion time is 45-55 s.
6. The biological agent for preventing and treating Dendrobium wilt according to claim 1, characterized in that: In step (2), the temperature for heating and stirring is 60-70℃, and the stirring speed is 120-140r / min.
7. The biological agent for controlling Dendrobium twig blight according to claim 1, characterized in that: In step (3), the stirring speed is 80-100 r / min and the stirring time is 10-15 min.