A mesocarpium crystal mask composition containing nanovesicles, a mesocarpium crystal mask and a preparation method thereof

Abstract

The application discloses a kind of containing nanovesicle's mesona hexapetala water crystal mask composition, water crystal mask and preparation method thereof, belong to cosmetic technical field;The composition provided by the application includes the following components by mass fraction: mesona hexapetala extract 0.5-2 parts, radix rehmanniae preparata extract 0.05-0.2 parts, plum blossom extract 0.05-0.2 parts, biomimetic nanovesicle 0.2-2 parts, gum substance 0.8-2 parts, humectant 0.1-8 parts, preservative 0.4-2 parts, auxiliary agent 0.05-0.2 parts, water 60-98 parts.The composition provided by the application has excellent anti-inflammatory soothing effect, antioxidant and anti-photoaging effect;And the water crystal mask provided by the application can maintain fixed shape, suitable for sensitive skin population;In addition, the preparation method of the mesona hexapetala water crystal mask composition containing nanovesicle provided by the application is simple to operate, does not need special equipment, and is beneficial to actual production application.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and particularly relates to a crystal mask composition containing nanovesicles of jelly grass, the crystal mask, and its preparation method. Background Technology

[0002] Face masks primarily work by temporarily isolating the skin from external air and pollution during the short period of time they are applied. This raises skin temperature, dilates pores, promotes sweat gland secretion and metabolism, and increases the skin's oxygen content. This helps the skin eliminate metabolic waste products and accumulated oils from epidermal cells. The moisture in the mask penetrates the stratum corneum of the skin's epidermis, making the skin soft, naturally radiant, and elastic. Other active ingredients also penetrate the stratum corneum, bringing corresponding effects.

[0003] Generally, cosmetic face masks are manufactured by adding water or lotion to non-woven fabric, or by adding extracts with known functional properties. Furthermore, to overcome the shortcomings of non-woven fabric masks, significant research and development has been undertaken on crystal face masks. To maintain the mask's shape, hydrogels are typically attached to synthetic resins, non-woven fabrics, meshes, or nets. However, hydrogels often detach from the non-woven fabric, and when using hydrogels made of synthetic resins, issues may arise regarding biocompatibility and stability.

[0004] As a result, in order to overcome the aforementioned issues such as biocompatibility, extensive research is being conducted on crystal masks utilizing substances derived from nature. In the case of natural crystal masks, because non-woven fabrics or synthetic resins are not used, but rather the film is made directly from substances obtained from nature, they not only avoid the side effects such as itching associated with sensitive skin caused by non-woven fabrics or synthetic resins, but also provide direct nutrition to the skin. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a nano-vesicle-containing herbal crystal mask composition, crystal mask and preparation method thereof, which is suitable for sensitive skin, can repair sensitive skin, and has antioxidant, anti-inflammatory and soothing, anti-photoaging and whitening effects.

[0006] To achieve the above objectives, in a first aspect, the present invention provides a jelly grass crystal mask composition containing nanovesicles, the jelly grass crystal mask composition containing nanovesicles comprising the following components in parts by weight:

[0007] Mesona chinensis extract 0.5-2 parts, Rehmannia glutinosa extract 0.05-0.2 parts, Prunus mume extract 0.05-0.2 parts, biomimetic nanovesicles 0.2-2 parts, gelatinous substances 0.8-2 parts, humectant 0.1-8 parts, preservative 0.4-2 parts, adjuvant 0.05-0.2 parts, water 60-98 parts;

[0008] The gum-like substance is at least one of carrageenan, guar gum, locust bean gum, gelatin, konjac flour, gum arabic, starch, guar gum, alginic acid, xanthan gum, glucomannan, pectin, and cellulose.

[0009] The raw materials for preparing the biomimetic nanovesicles include phospholipids, cholesterol, superoxide dismutase, sodium ribonucleic acid, and water.

[0010] This invention provides a crystal mask composition containing nanovesicles and jelly grass extract. By selecting specific components for combined use, especially by adding biomimetic nanovesicles, jelly grass extract, Rehmannia glutinosa extract, and plum blossom extract with specific raw materials, the four components have a significant synergistic effect, achieving excellent anti-inflammatory and soothing effects, antioxidant and anti-photoaging effects. Furthermore, by selectively adding appropriate amounts of specific types of gelatinous substances and biomimetic nanovesicles, this invention ensures that the resulting crystal mask maintains a fixed shape, avoiding the introduction of non-woven fabric in the subsequent mask preparation, thus making it suitable for people with sensitive skin.

[0011] Among them, the main components and mechanisms of the herbal jelly crystal mask composition containing nanovesicles that have antioxidant, anti-photoaging, soothing and anti-inflammatory effects are as follows:

[0012] Mesona chinensis extract: Mesona chinensis extract is obtained by extracting mesona chinensis, an important medicinal and edible oriental plant resource with the effects of "clearing summer heat, relieving internal heat, clearing heat toxins, and treating alcohol-related ailments". Mesona chinensis extract has antioxidant and antibacterial effects.

[0013] Rehmannia glutinosa extract: Rehmannia glutinosa extract has the effects of clearing heat and promoting body fluid production, cooling the body, and stopping bleeding;

[0014] Plum blossom extract: Winter plum is slightly cooling in nature and has the properties of clearing heat and detoxifying. When used externally, it can kill bacteria, regulate skin inflammation, balance oil secretion, and help skin metabolism. It has a good effect on improving closed comedones and oily acne-prone skin.

[0015] Bionic nanovesicles: The sodium ribonucleic acid (RNA-Na) in biomimetic nanovesicles has a certain stimulating effect on human keratinocytes (a special type of epidermal cell that synthesizes keratin), which can regenerate and renew skin cells, making the complexion rosy and moisturized, improving skin function, repairing and healing damaged skin, promoting hair growth and preventing hair loss, and having anti-ultraviolet, radiation, and radioactive effects. Therefore, it can be used as an additive in moisturizing, conditioning, whitening, and anti-aging cosmetics. The superoxide dismutase (SOD) in biomimetic nanovesicles can effectively remove free radicals in the body and specifically eliminate the damage of superoxide free radicals to cells, thereby achieving the effects of anti-oxidation, anti-inflammation, anti-aging, and balancing metabolism. The cholesterol and phospholipids in biomimetic nanovesicles have moisturizing effects. Preparing phospholipids, cholesterol, superoxide dismutase, sodium ribonucleic acid, and water into biomimetic nanovesicles can ensure the activity of superoxide dismutase and sodium ribonucleic acid during subsequent preparation processes, and can also have good compatibility with other components, further enhancing the moisturizing, anti-oxidation, and anti-inflammatory effects.

[0016] As a preferred embodiment of the jelly grass crystal mask composition containing nanovesicles according to the present invention, the jelly grass crystal mask composition containing nanovesicles comprises the following components in parts by weight: 0.8-1.5 parts of jelly grass extract, 0.08-0.15 parts of Rehmannia glutinosa extract, 0.08-0.15 parts of plum blossom extract, 0.8-1.5 parts of biomimetic nanovesicles, 1.2-1.8 parts of gelatinous substances, 4-6 parts of moisturizer, 0.8-1.2 parts of preservative, 0.08-0.12 parts of auxiliary agent, and 70-98 parts of water.

[0017] As a preferred embodiment of the jelly grass crystal mask composition containing nanovesicles according to the present invention, the jelly grass crystal mask composition containing nanovesicles comprises the following components in parts by weight: 1 part jelly grass extract, 0.1 part Rehmannia glutinosa extract, 0.1 part plum blossom extract, 1 part biomimetic nanovesicles, 1.5 parts gelatinous substance, 5 parts moisturizer, 1 part preservative, 0.1 part auxiliary agent, and 90.2 parts water.

[0018] The inventors discovered that the mass fraction of the components in the herbal jelly crystal mask composition containing nanovesicles has a significant impact on the overall effect of the product. When the mass fraction of the components in the herbal jelly crystal mask composition containing nanovesicles is further selected within the above-mentioned small range, especially the above-mentioned point values, the overall effect of the product is the best.

[0019] As a preferred embodiment of the herbal jelly crystal mask composition containing nanovesicles described in this invention, the mass ratio of phospholipids, cholesterol, sodium ribonucleic acid, superoxide dismutase and water in the raw materials for preparing the biomimetic nanovesicles is phospholipids: cholesterol: superoxide dismutase: sodium ribonucleic acid: water = (14-16): (2-4): (1-2): (0.3-0.7): (75-85).

[0020] Preferably, in the raw materials for preparing the biomimetic nanovesicles, the mass ratio of phospholipids, cholesterol, sodium ribonucleic acid, superoxide dismutase and water is phospholipid:cholesterol:superoxide dismutase:sodium ribonucleic acid:water = 15:3:1.5:0.5:80.

[0021] The inventors discovered that when the mass ratio of the substances in the biomimetic nanovesicles is further selected within the above range, a vesicle-like structure can be prepared better, resulting in better structural stability. Furthermore, when the nanovesicles are subsequently added to the herbal jelly crystal mask composition containing nanovesicles, the overall effect of the resulting herbal jelly crystal mask composition is more superior.

[0022] As a preferred embodiment of the herbal jelly crystal mask composition containing nanovesicles described in this invention, the preparation method of the biomimetic nanovesicles is as follows: phospholipids, cholesterol, sodium ribonucleic acid, superoxide dismutase and water are mixed, homogenized under pressure, and then extruded to obtain biomimetic nanovesicles.

[0023] Preferably, the homogenization is performed twice; the pressure applied is 80-150 MPa.

[0024] Preferably, the extrusion is performed by repeatedly extruding the solution in a liposome extruder, causing the solution to repeatedly pass through the filter membrane inside the liposome extruder under extrusion, thereby forming a vesicle-like structure; the number of repeated extrusions is 2-4 times.

[0025] As a preferred embodiment of the herbal jelly crystal mask composition containing nanovesicles according to the present invention, the moisturizer includes at least one of 1,3-butanediol, ethylene glycol, propylene glycol, hexanediol, sorbitol, mannitol, polyethylene glycol, polypropylene glycol, polyvinyl alcohol, and hyaluronic acid.

[0026] Preferably, the humectant is propylene glycol.

[0027] In a preferred embodiment of the jelly grass crystal mask composition containing nanovesicles described in this invention, the additive is potassium chloride and / or potassium hydroxide.

[0028] In a preferred embodiment of the jelly grass crystal mask composition containing nanovesicles according to the present invention, the preservative includes at least one of p-hydroxyacetophenone, 1,2-hexanediol, and methyl ester.

[0029] Preferably, the preservative is p-hydroxyacetophenone and 1,2-hexanediol.

[0030] In a second aspect, the present invention provides a method for preparing the aforementioned jelly-like herbal crystal mask composition containing nanovesicles, the method comprising the following steps:

[0031] (1) Dissolve phase A by heating at 50-60℃;

[0032] (2) Mix phase B and stir until homogeneous;

[0033] (3) Add phase B to phase A and heat to 80-90℃ to completely dissolve it. Keep warm and stir for 20-30 minutes until it is uniformly swollen and then homogenize for 25-35 seconds.

[0034] (4) After cooling to 65-75℃, add the dissolved C phase and stir evenly to obtain the hydrogel blank solution;

[0035] (5) Keep the hydrogel blank liquid at a temperature of 65-70℃, add phase D quickly, stir for 30-50s and pour into a roller coater to form a flat sheet, then cool down to -10-4℃ for cooling and solidification, and then cut into the shape of a face mask to obtain a jelly grass crystal face mask composition containing nanovesicles.

[0036] Phase A consists of water and additives;

[0037] Phase B consists of humectants and gelling agents;

[0038] Phase C is a preservative;

[0039] The D phase consists of extracts of licorice root, prepared rehmannia root, plum blossom, and biomimetic nanovesicles.

[0040] Preferably, the cooling and curing is carried out in a cooling machine.

[0041] The preparation method of the present invention can be used to prepare a mask material of any shape from a jelly grass crystal mask composition containing nanovesicles.

[0042] In a third aspect, the present invention provides a crystal mask comprising a mask material and a mask liquid, wherein the mask material is the jelly grass crystal mask composition containing nanovesicles described in the present invention, and the mask liquid is a cosmetic moisturizer or water.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0044] This invention provides a herbal jelly crystal mask composition containing nanovesicles. By selecting specific components for combined use, it exhibits excellent anti-inflammatory and soothing effects, as well as antioxidant and anti-photoaging effects. Furthermore, by selectively adding appropriate amounts of specific types of gelatinous substances and biomimetic nanovesicles, this invention ensures that the resulting crystal mask maintains a fixed shape, avoiding the introduction of non-woven fabric in subsequent mask preparation, thus making it suitable for people with sensitive skin. In addition, the preparation method of the herbal jelly crystal mask composition containing nanovesicles provided by this invention is simple to operate, requires no special equipment, and is beneficial for practical production applications. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] Unless otherwise specified, the raw materials used in this invention are conventional commercially available raw materials, and the raw materials used in the parallel embodiments or comparative examples of this invention are the same.

[0047] The extracts of Mesona chinensis, Rehmannia glutinosa, and Prunus cerasifera used in this invention are from Guangzhou Yuanxiang Medical Biotechnology Co., Ltd. The preparation method is as follows: the medicinal materials are dried, pulverized and sieved, defatted with 95% ethanol, then soaked at room temperature for 2-12 hours, filtered, the filter residue is collected and heated for extraction, centrifuged after extraction, and the supernatant is collected to obtain the corresponding extracts.

[0048] Bionic nanovesicle 1: Phospholipids, cholesterol, SOD, RNA-Na, and water were dissolved in a ratio of phospholipids:cholesterol:superoxide dismutase:sodium ribonucleic acid:water = 15:3:1.5:0.5:80. The solution was homogenized twice under a pressure of 120MPa to form an initial vesicle solution. The solution was then added to a liposome extruder and repeatedly squeezed three times. The solution was repeatedly squeezed through the filter membrane in the liposome extruder to form a vesicle-like structure, thus obtaining bionic nanovesicle 1.

[0049] Bionic nanovesicle 2: The only difference between it and bionic nanovesicle 1 is that the ratio of phospholipid:cholesterol:superoxide dismutase:sodium ribonucleic acid:water is 10:5:3:3:80.

[0050] Examples 1-9 and Comparative Examples 1-9

[0051] Examples 1-9 and Comparative Examples 1-9 of the present invention provide a jelly grass crystal mask composition containing nanovesicles, and the component content (parts by mass) of the jelly grass crystal mask composition containing nanovesicles is shown in Table 1-2.

[0052] Table 1

[0053]

[0054] Table 2

[0055]

[0056]

[0057] The preparation method of the herbal jelly crystal mask composition containing nanovesicles provided in Example 1 is as follows:

[0058] (1) Dissolve phase A by heating at 50-60℃;

[0059] (2) Mix phase B and stir until homogeneous;

[0060] (3) Add phase B to phase A and heat to 80-90℃ to completely dissolve it. Keep warm and stir for 25 minutes until it is uniformly swollen and then homogenize for 30 seconds.

[0061] (4) After cooling to 65-75℃, add the dissolved C phase and stir evenly to obtain the hydrogel blank solution;

[0062] (5) Keep the hydrogel blank solution at a temperature of 65-70℃, add phase D quickly, stir for 40s and pour into a roller coater to form a flat sheet. Then cool it down to -10-4℃ in a cooler for cooling and solidification, and then cut it into a mask shape to obtain a jelly grass crystal mask composition containing nanovesicles.

[0063] Phase A consists of water and additives;

[0064] Phase B consists of humectants and gelling agents;

[0065] Phase C is a preservative;

[0066] The D phase consists of extracts of licorice root, prepared rehmannia root, plum blossom, and biomimetic nanovesicles.

[0067] The preparation methods of the herbal jelly crystal mask compositions containing nanovesicles provided in Examples 2-9 and Comparative Examples 1-9 of this invention are consistent with those in Example 1, except that the relevant components are not added.

[0068] Comparative Example 10

[0069] The present invention provides a comparative example of a mesona chinensis crystal mask composition containing nanovesicles. The only difference between the mesona chinensis crystal mask composition containing nanovesicles and Example 1 is that peppermint extract is used instead of mesona chinensis extract.

[0070] Comparative Example 11

[0071] The present invention provides a comparative example of a herbal crystal mask composition containing nanovesicles, the only difference between the herbal crystal mask composition containing nanovesicles and Example 1 is that the extract of Rehmannia glutinosa is replaced with the extract of Ophiopogon japonicus root.

[0072] Comparative Example 12

[0073] The present invention provides a comparative example of a herbal crystal mask composition containing nanovesicles, the only difference between the herbal crystal mask composition containing nanovesicles and Example 1 is that fig leaf extract is used instead of plum blossom extract.

[0074] Example 1

[0075] The anti-inflammatory effects of the jelly grass crystal mask compositions containing nanovesicles prepared in Examples 1-9 and Comparative Examples 1-12 were verified by the following tests:

[0076] 1. Verification of anti-inflammatory effects at the cellular level

[0077] Anti-inflammatory assay based on LPS-induced RAW264.7 cell inflammation model: Mode (model group) l: 200 μL PBS and LPS (final concentration 1 μg / mL) were added to 6-well plates; Control (blank control group): 200 μL PBS was added to 6-well plates;

[0078] Test sample: Add 200 μL of filtered test sample and LPS (final concentration 1 μg / mL) to a 6-well plate;

[0079] Each treatment group was set up with 3 replicates. After incubation for 24 hours, the cell supernatant was collected and the procedure was performed according to the ELISA kit instructions. The average IL-6 expression level was obtained.

[0080] The results are shown in Table 3.

[0081] Table 3

[0082]

[0083] Wherein, *** indicates that the example or comparative example is significantly different from the model group, P < 0.001.

[0084] As shown in Table 3, compared with the blank control group, the expression level of the inflammatory factor IL-6 induced by LPS was significantly increased in the model group (P<0.001), indicating that LPS-induced modeling was successful.

[0085] As can be seen from Table 3, the product obtained by using the technical solution of the present invention can significantly reduce the expression level of the inflammatory factor IL-6. Compared with the model group, the products prepared in Examples 1-9 show a significant difference in IL-6 expression level, that is, the herbal jelly crystal mask composition containing nanovesicles prepared by the preparation method of the present invention has a soothing effect; especially when the component content and component type in Examples 1-3 are further selected, the anti-inflammatory effect of the obtained product is better, and the expression level of IL-6 is below 2.65 ng / mL;

[0086] As can be seen from Examples 1 and Comparative Examples 1-4, when the mass fraction of any one of the following extracts—grass jelly extract, rehmannia root extract, plum blossom extract, and biomimetic nanovesicles—is outside the scope of this invention, the inhibitory effect of the obtained product on IL-6 expression level is significantly reduced. As can be seen from Examples 1 and Comparative Examples 5-8, when any one of the following extracts—grass jelly extract, rehmannia root extract, plum blossom extract, and biomimetic nanovesicles—is not added, but the total amount remains unchanged, the anti-inflammatory effect of the obtained product is also significantly reduced. As can be seen from Examples 1 and Comparative Examples 10-12, when other ingredients are used to replace the extracts of the following extracts—grass jelly extract, rehmannia root extract, or plum blossom extract—the inhibitory effect of the obtained product on IL-6 expression level is significantly reduced. As can be seen from Examples 1, Examples 7-8, and Comparative Example 9, changing the gelling material also affects the anti-inflammatory effect; when the selection of the gelling material is outside the scope of this invention, excellent anti-inflammatory effects cannot be obtained.

[0087] 2. Human applicability and soothing test

[0088] 1) Relief effect on histamine-induced itching

[0089] The crystal masks prepared in Examples 1, 2, 4, 6, 8 and Comparative Example 1 were cut into 4×4cm pieces. Twenty-four test subjects were randomly divided into six groups of four. Each test subject took a 4×4cm area from the same part of their left and right hands, serving as the test group and the blank control group, respectively. Three drops of histamine solution (1.3%) were applied to each area until fully absorbed. After one minute, the test group applied the cut-out test sample; the blank control group received no application and served as a blank control. Test subjects conducted sensory evaluations, observing and experiencing the difference in itch relief time between the test group and the control group. The itch relief time was recorded, and the average value was calculated.

[0090] 2) Soothing effect on redness and rashes caused by capsaicin

[0091] The crystal masks prepared in Examples 1, 2, 4, 6, 8 and Comparative Example 1 were cut into 4×4cm pieces. Twenty-four test subjects were randomly divided into six groups of four. Each test subject took a 4×4cm area from the same part of their left and right hands, designated as the test group and the blank control group, respectively. Three drops of capsaicin solution (5.0%) were applied to each area until fully absorbed. After one minute, the test group was applied with the cut test sample; the blank control group was left untreated. Test subjects performed sensory evaluations, observing and experiencing the difference in redness and swelling reduction time between the test group and the control group; the time for redness and rash to fade was recorded, and the average value was calculated.

[0092] The records of the time for itch relief, redness reduction, and rash disappearance are shown in Table 4.

[0093] Table 4

[0094]

[0095] As can be seen from Table 4, in human experiments, the technical solution of the present invention can significantly shorten the time for relieving itching, redness, and rash compared to the control group, with an anti-itch time of less than 10 minutes, a redness fading time of less than 16 minutes, and a rash fading time of less than 16 minutes.

[0096] As can be seen from Example 1 and Comparative Example 1, when the mass fraction of the grass jelly extract is outside the scope of the present invention, it is impossible to effectively achieve a soothing effect. Compared with Example 1, the antipruritic time of Comparative Example 1 increased by 2 times, the redness fading time increased by 1 time, and the rash fading time increased by 1.5 times.

[0097] Example 2

[0098] The effects of this invention are verified by examples of the stimulation inhibition of the jelly grass crystal mask compositions containing nanovesicles prepared in Examples 1-9 and Comparative Examples 1-12. The method is as follows: ECVAM-DB-ALM: INVITTOX protocol: Red Blood Cell Test System INVITTOX n37.

[0099] The specific test is as follows:

[0100] The surfactant (0.3% sodium lauryl sulfate), the preservative (0.3% isothiazolinone), and the fragrance (30% lemon oil) were used as irritants to stimulate hemolysis of red blood cells.

[0101] 1. Test samples (Examples 1-9, Comparative Examples 1-9) were pre-added to the RBC Test System, and the above-mentioned stimuli were added. The effects of different test samples on hemolysis of red blood cells caused by the above substances were tested respectively.

[0102] 2. Calculation formula

[0103] 1) Red blood cell hemolysis rate = (OD sample group - OD solvent control group) / (OD model control group - OD solvent control group) * 100%;

[0104] The model control was a complete hemolysis control, which was distilled water, and the solvent control was PBS;

[0105] 2) Red blood cell hemolysis inhibition rate = (hemolysis rate of negative group - hemolysis rate of sample group) / hemolysis rate of negative group * 100%;

[0106] The negative group was the control group to which the stimulant was added;

[0107] The results of the hemolysis inhibition rate obtained by the test are shown in Tables 5-7; Table 5 shows the effect of the test sample on hemolysis induced by sodium dodecyl sulfate, Table 6 shows the effect of the test sample on hemolysis induced by isothiazolinone, and Table 7 shows the effect of the test sample on hemolysis induced by lemon oil.

[0108] Table 5

[0109] Inhibition rate (%) Inhibition rate (%) Inhibition rate (%) Blank control group 0.00 Example 7 69.87 Comparative Example 5 36.98 Example 1 84.21 Example 8 68.43 Comparative Example 6 37.28 Example 2 74.56 Example 9 82.78 Comparative Example 7 40.56 Example 3 75.31 Comparative Example 1 43.64 Comparative Example 8 40.32 Example 4 71.59 Comparative Example 2 35.78 Comparative Example 9 35.89 Example 5 71.32 Comparative Example 3 46.12 Comparative Example 10 38.92 Example 6 74.58 Comparative Example 4 44.23 Comparative Example 11 36.71 Comparative Example 12 34.26

[0110] Table 6

[0111] Inhibition rate (%) Inhibition rate (%) Inhibition rate (%) Blank control group 0.11 Example 7 67.33 Comparative Example 5 40.18 Example 1 86.14 Example 8 65.12 Comparative Example 6 38.14 Example 2 73.06 Example 9 87.08 Comparative Example 7 39.76 Example 3 72.61 Comparative Example 1 33.24 Comparative Example 8 43.05 Example 4 69.09 Comparative Example 2 41.23 Comparative Example 9 37.76 Example 5 70.14 Comparative Example 3 47.12 Comparative Example 10 37.02 Example 6 74.23 Comparative Example 4 45.22 Comparative Example 11 35.65 Comparative Example 12 34.91

[0112] Table 7

[0113]

[0114]

[0115] As can be seen from Tables 5-7, when the technical solution of the present invention is adopted, the hemolysis of erythrocytes caused by sodium dodecyl sulfate, isothiazolinone, and lemon oil stimulation can be significantly reduced. Specifically, the inhibition rate of hemolysis of erythrocytes caused by sodium dodecyl sulfate stimulation is above 68.43%, the inhibition rate of hemolysis of erythrocytes caused by isothiazolinone stimulation is above 65.12%, and the inhibition rate of hemolysis of erythrocytes caused by lemon oil stimulation is above 66.23%. When the mass fraction of the components, the selection of the components are not within the scope of the present invention, or when a certain component is not added, the inhibition rate of hemolysis of erythrocytes of the obtained product is significantly reduced.

[0116] Example 3: Tyrosinase Activity Assay

[0117] This experiment evaluated the anti-photoaging effect by measuring the inhibition rate of the sample on L-tyrosinase activity.

[0118] Test samples: Water was used as a blank control, and the nanovesicle-containing herbal crystal mask compositions obtained in Examples 1-3 and Comparative Examples 1-2 were tested simultaneously.

[0119] Experimental procedure:

[0120] A. B16 mouse melanoma cells were injected at a rate of 8 × 10⁻⁶ 3 Cells were seeded at 100 μL per well in a 96-well plate and allowed to adhere at 37°C for 24 hours.

[0121] B. After 24 hours, the cells of different sample specimens were treated with Examples 1-3 and Comparative Examples 1-2 for 48 hours respectively.

[0122] C. To measure cellular tyrosinase activity, cells were washed with ice-cold PBS, then lysed with 90 μL of PBS containing 1% Triton-X100. The lysate was then centrifuged at 500 x g for 15 min at 4 °C, and the protein concentration was determined using a BCA protein assay kit (P0011, Baguio Biotechnology Institute, China).

[0123] D. Add 200 μL of the mixture containing 40 μg of protein to each well of a 96-well plate and incubate at 37°C with 2.5 mM 3,4-dihydroxy-L-phenylalanine (L-DOPA) for 1 hour. Measure the absorbance at 490 nm using a microplate reader. The results are shown in Table 8.

[0124] Table 8

[0125]

[0126]

[0127] As can be seen from Table 8, the tyrosinase activity of the product obtained by using the technical solution of the present invention is low, ranging from 3.562 to 5.664 U / g, which is 66.45-78.92% lower than that of the blank control. However, when the amount of the added component is not within the range given in the present invention, the tyrosinase activity of the obtained product increases significantly, that is, the anti-photoaging effect decreases.

[0128] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A crystal mask composition containing nanovesicles of jelly grass, characterized in that, The herbal jelly crystal mask composition containing nanovesicles comprises the following components in parts by weight: Mesona chinensis extract 0.5-2 parts, Rehmannia glutinosa extract 0.05-0.2 parts, Prunus mume extract 0.05-0.2 parts, biomimetic nanovesicles 0.2-2 parts, gelatinous substances 0.8-2 parts, humectant 0.1-8 parts, preservative 0.4-2 parts, adjuvant 0.05-0.2 parts, water 60-98 parts; The gum-like substance is at least one of carrageenan, konjac flour, and glucomannan; The raw materials for preparing the biomimetic nanovesicles include phospholipids, cholesterol, superoxide dismutase, sodium ribonucleic acid, and water. In the raw materials for preparing the biomimetic nanovesicles, the mass ratio of phospholipids, cholesterol, sodium ribonucleic acid, superoxide dismutase, and water is phospholipids:cholesterol:superoxide dismutase:sodium ribonucleic acid:water = (14-16):(2-4):(1-2):(0.3-0.7):(75-85); The extracts of Mesona chinensis, Rehmannia glutinosa, and Prunus mume were sourced from Guangzhou Yuanxiang Medical Biotechnology Co., Ltd.

2. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The herbal jelly crystal mask composition containing nanovesicles comprises the following components in parts by weight: Mesona chinensis extract 0.8-1.5 parts, Rehmannia glutinosa extract 0.08-0.15 parts, Prunus mume extract 0.08-0.15 parts, biomimetic nanovesicles 0.8-1.5 parts, gelatinous substances 1.2-1.8 parts, humectant 4-6 parts, preservative 0.8-1.2 parts, adjuvant 0.08-0.12 parts, water 70-98 parts.

3. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The herbal jelly crystal mask composition containing nanovesicles comprises the following components in parts by weight: Mesona chinensis extract 1 part, Rehmannia glutinosa extract 0.1 part, Prunus mume extract 0.1 part, biomimetic nanovesicles 1 part, gelatinous substances 1.5 parts, humectant 5 parts, preservative 1 part, adjuvant 0.1 part, water 90.2 parts.

4. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The preparation method of the biomimetic nanovesicles is as follows: phospholipids, cholesterol, sodium ribonucleic acid, superoxide dismutase and water are mixed, homogenized under pressure, and then extruded to obtain biomimetic nanovesicles.

5. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The moisturizer includes at least one of 1,3-butanediol, ethylene glycol, propylene glycol, hexanediol, sorbitol, mannitol, polyethylene glycol, polypropylene glycol, polyvinyl alcohol, and hyaluronic acid.

6. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The auxiliary agent is potassium chloride and / or potassium hydroxide.

7. The herbal jelly crystal mask composition containing nanovesicles according to claim 1, characterized in that, The preservative includes at least one of p-hydroxyacetophenone, 1,2-hexanediol, and methyl ester.

8. The method for preparing the herbal jelly crystal mask composition containing nanovesicles as described in any one of claims 1-7, characterized in that, The preparation method includes the following steps: (1) Dissolve phase A by heating at 50-60℃; (2) Mix phase B thoroughly; (3) Add phase B to phase A and heat to 80-90℃ to dissolve it completely. Keep it warm and stir for 20-30 minutes until it is uniformly swollen and then homogenize for 25-35 seconds. (4) After cooling to 65-75℃, add the dissolved C phase and stir evenly to obtain the hydrogel blank solution; (5) Keep the hydrogel blank solution at a temperature of 65-70℃, add phase D quickly, stir for 30-50s and pour into a roller coater to form a flat sheet, then cool down to -10-4℃ for cooling and solidification, and then cut into the shape of a face mask to obtain a jelly grass crystal face mask composition containing nanovesicles. Phase A consists of water and additives; Phase B consists of humectants and gelling agents; Phase C is a preservative; The D phase consists of extracts of licorice root, prepared rehmannia root, plum blossom, and biomimetic nanovesicles.

9. A crystal facial mask, characterized in that, The crystal mask comprises a mask material and a mask liquid, wherein the mask material is a jelly grass crystal mask composition containing nanovesicles as described in any one of claims 1-7, and the mask liquid is a cosmetic moisturizer or water.

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