A high specific activity alkaline xylanase mutant
By genetically modifying alkaline xylanase and designing specific amino acid substitution sites, its specific activity was improved, solving the problems of low specific activity and high cost of existing alkaline xylanases in industrial applications, and enabling wider industrial applications.
CN117384886BActive Publication Date: 2026-07-24QINGDAO VLAND BIOTECH GRP CO LTD
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Patent Information
- Application Number
- CN202311342665.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-07-12
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2042-07-12
AI Technical Summary
Technical Problem
Existing alkaline xylanases have problems such as low enzyme activity, instability, and high cost in industrial applications, making it difficult to meet production needs.
Method used
By genetically engineering alkaline xylanase, mutants with specific amino acid substitutions were designed and constructed, including mutants at sites such as I37V, A59S, D63E, D104Y, T107M/K, N167G, and D192E, to improve its specific activity.
Benefits of technology
The specific activity of the mutants was significantly increased, generally by 8.5%-32.8%, especially the D192E single-point mutant, which had the highest specific activity of 1625.11 U/mg, reducing production costs and promoting its application in the industrial field.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application relates to the field of genetic engineering and protein modification technology, and particularly relates to a high specific activity basic xylanase mutant and application thereof. The present application takes wild type xylanase H1 as a basis, and provides a mutant containing at least one of the following mutation sites: I37V, A59S, D63E, D104Y, T107M / K, N167G and D192E. Compared with the wild type xylanase H1, the specific activity of the xylanase mutant provided by the present application is generally increased by 8.5%-32.8%; wherein the specific activity of the xylanase mutant containing the D192E single point mutation is the highest, reaching 1625.11 U / mg, which is conducive to reducing the production cost of the enzyme and promoting its wide application in the industrial field.
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