Conjugated polymer-based activatable acoustically powered nanomaterials, methods of making and using the same

Abstract

The application belongs to the technical field of near-infrared luminescence and ultrasonic treatment materials, discloses a kind of activatable acoustic power nano materials based on conjugated polymer and its preparation method and application, comprising the following steps: 2,6-di (trimethyl tin) -4,4-di (2-ethylhexyl) -dithiophene and cyclopentadiene, 4,7-dibromo-5,6-dinitrobenzo [c] [1,2,5] thiazole, catalyst tetra (triphenylphosphine) palladium are added into reaction tube, toluene is added, and the reaction product is settled with methanol under nitrogen protection, to obtain conjugated polymer;Degradable mesoporous silica is used to wrap to realize the water-soluble of molecule, and the corresponding activatable acoustic treatment reagent is obtained.The material disclosed in the application can be used for activatable acoustic dynamics treatment guided by near-infrared two-zone imaging, which helps to improve the specificity of tumor treatment, and mesoporous silica wrapping can also be used for drug loading and transport.

Description

Technical Field

[0001] This invention relates to the technical field of near-infrared luminescence and ultrasound therapy materials, specifically to an activatable acoustic-dynamic nanomaterial based on conjugated polymers, its preparation method, and its applications. Background Technology

[0002] Among current optical diagnostic techniques, near-infrared II (NIR-II, 1000-1700nm) fluorescence imaging technology boasts greater penetration depth, higher resolution, and higher sensitivity. Furthermore, it increases the signal-to-background ratio by reducing photon scattering and tissue autofluorescence, making it a highly promising optical imaging diagnostic technique. Optical imaging is often used in conjunction with phototherapy; however, phototherapy's limited penetration depth significantly restricts its application. Ultrasound differs from light, penetrating to depths of several centimeters within living tissue, enabling the treatment of deep-seated diseases. Therefore, in recent years, the use of ultrasound to activate sonosensitive agents to generate reactive oxygen species (sonic dynamics therapy) has attracted widespread attention. However, highly effective sonosensitive agents are currently scarce, and these agents are mostly always-on, potentially generating reactive oxygen species in normal tissues during use, leading to side effects. Additionally, there are currently no reports of NIR-II luminescent sonosensitive agents. Therefore, NIR-II luminescent sonosensitive agents that can be activated at open lesion sites are of great significance for efficient and highly specific diagnosis and treatment.

[0003] Conjugated polymers have been widely used in near-infrared II fluorescence imaging and photodynamic therapy due to their tunable band structure, good biocompatibility, and ease of metabolism in biological systems. However, sonosensitive agents based on conjugated polymers, especially activatable sonosensitive agents, have not yet been reported. Therefore, developing novel materials based on conjugated polymers to achieve near-infrared II imaging-guided activatable sonodynamic therapy is of great value for high-resolution optical diagnosis and highly specific targeted therapy, but no relevant materials have been reported to date. Summary of the Invention

[0004] To address the shortcomings of the aforementioned technologies, this invention provides an activatable sonodynamic nanomaterial based on conjugated polymers, its preparation method, and its applications. The resulting material exhibits near-infrared fluorescence and photodynamic therapy capabilities in the 700-1200 nm range and can be degraded by high concentrations of glutathione within tumors, thereby restoring its sonodynamic therapy capabilities. It can be used for near-infrared II imaging-guided activatable sonodynamic therapy, which helps improve the specificity of tumor treatment. Furthermore, the mesoporous silica encapsulation can also be used for drug loading and delivery.

[0005] The technical solution provided by this invention is as follows:

[0006] This invention uses 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienenocyclopentadiene and 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole as monomers to obtain a conjugated polymer with near-infrared fluorescence emission through polymerization. This novel molecule can also generate singlet oxygen under the action of an ultrasound therapy device for sonodynamic therapy. The specific reaction pathway is as follows:

[0007]

[0008] To address the hydrophobicity of this molecule and obtain an activatable sonodynamic therapeutic material, it was modified with biodegradable mesoporous silica, resulting in an activatable sonodynamic nanomaterial based on a conjugated polymer that can be used for near-infrared fluorescence imaging-guided sonodynamic therapy and drug delivery.

[0009] A method for preparing activatable acoustic-dynamic nanomaterials based on conjugated polymers includes the following steps:

[0010] 2,6-Di(trimethyltin)-4,4-Di(2-ethylhexyl)-dithienenocyclopentadiene, 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole and tetra(triphenylphosphine)palladium were added to a reaction tube, and toluene was added and reacted under nitrogen protection. The reaction product was precipitated with methanol, filtered and collected, and dried to obtain the conjugated polymer solid PCPDTBT-NO2.

[0011] Prepare a PCPDTBT-NO2 chloroform solution, mix it with an aqueous CTAB solution, emulsify it with ultrasound, and remove the chloroform to obtain an aqueous solution of the conjugated polymer;

[0012] An aqueous solution of the conjugated polymer was mixed with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and an aqueous solution of sodium hydroxide and reacted. Triethoxysilane-modified polyethylene glycol was added and the reaction continued. After purification by ultrafiltration, an activatable sonodynamic nanomaterial based on the conjugated polymer was obtained.

[0013] Furthermore, the molar ratio of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienenocyclopentadiene and 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole is 1:1.

[0014] Further, 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienenocyclopentadiene, 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, and tetra(triphenylphosphine)palladium were added to a reaction tube, toluene was added under nitrogen protection, and the mixture was placed in an oil bath at 100°C for 8-48 hours.

[0015] Furthermore, the reaction product was precipitated with methanol, filtered and collected, and dried at 50-60℃ to obtain the conjugated polymer solid PCPDTBT-NO2.

[0016] Furthermore, the concentration of PCPDTBT-NO2 in the PCPDTBT-NO2 chloroform solution is 1 mg / mL; the concentration of CTAB in the CTAB aqueous solution is 20 mg / mL; and the volume ratio of PCPDTBT-NO2 chloroform solution to CTAB aqueous solution is 1:8 to 12.

[0017] Further, a PCPDTBT-NO2 chloroform solution was prepared, mixed with an aqueous CTAB solution, and ultrasonically emulsified. The chloroform was then removed by heating at 60°C to obtain an aqueous solution of the conjugated polymer.

[0018] Furthermore, the volume ratio of the aqueous solution of the conjugated polymer to water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and sodium hydroxide aqueous solution is 1:9:0.03:0.05:0.2:0.06.

[0019] Furthermore, the aqueous solution of the conjugated polymer was mixed with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and sodium hydroxide aqueous solution. After reacting at 70°C for 15–60 min, polyethylene glycol modified with triethoxysilane was added to continue the reaction. After purification by ultrafiltration, the activated acoustic nanomaterial based on the conjugated polymer was obtained.

[0020] An activatable acoustic-dynamic nanomaterial based on a conjugated polymer and its preparation method, comprising the following steps:

[0021] Step 1: Synthesis of Conjugated Polymers

[0022] Step 1.1 Weigh 72.8 mg (0.1 mmol) of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienrocyclopentadiene, the same molar amount of 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, and 10 mg of tetra(triphenylphosphine)palladium, add them to a reaction tube, add 8 mL of toluene under nitrogen protection, and place the tube in an oil bath at 100 °C for 24 h; the molar ratio of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienrocyclopentadiene, 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, tetra(triphenylphosphine)palladium and toluene is 1:1:0.03~0.1:500~1000.

[0023] Step 1.2 The reaction product from Step 1.1 was precipitated with a large amount of methanol. After complete precipitation, the product was collected by filtration and dried at 50-60℃ to obtain the conjugated polymer solid, hereinafter referred to as PCPDTBT-NO2.

[0024] Step 2: Water-soluble modification of conjugated polymers

[0025] Prepare a 1 mg / mL PCPDTBT-NO2 chloroform solution, mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10, emulsify by sonication, and remove the chloroform by heating at 60°C to obtain an aqueous solution of the corresponding conjugated polymer.

[0026] Step 3 can activate the preparation of acoustic-dynamic nanomaterials.

[0027] The solution obtained in step 2 was mixed with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and 2 mol / L sodium hydroxide aqueous solution in a volume ratio of 1:9:0.03:0.05:0.2:0.06. After reacting at 70°C for about 40 min, triethoxysilane-modified polyethylene glycol was added and the reaction was continued for 3 h. After purification by ultrafiltration, water-soluble biodegradable mesoporous silica-encapsulated PCPDTBT-NO2 nanoparticles were obtained.

[0028] The present invention also provides an activatable acoustic-dynamic nanomaterial based on a conjugated polymer, which is prepared by the above method.

[0029] The present invention also provides the application of the above-mentioned conjugated polymer-based activatable acoustic dynamic nanomaterials in the preparation of near-infrared II luminescent acoustic sensitizer materials.

[0030] Beneficial effects

[0031] Conjugated polymers are a novel type of organic optoelectronic material. Their main chains typically possess alternating single, double, or triple bond structures and exhibit inherent semiconductor properties, hence they are also known as semiconductor polymers. Conjugated polymers possess excellent optical and electrical properties and are widely used in chemistry, materials science, and biology. In recent years, conjugated polymers have emerged as potential biomedical sonosensitive agents. Compared to inorganic materials and small organic dyes, conjugated polymers exhibit larger absorption coefficients, higher fluorescence quantum yields, excellent photostability, and biocompatibility. Furthermore, the spectrum of conjugated polymers can be tuned by altering the main chain structure, enabling them to exhibit near-infrared absorption or emission capabilities. To improve the water solubility of conjugated polymers, side chains can be directly modified to design water-soluble conjugated polymers beneficial for biological applications. Amphiphilic polymers can also be used to form nanoparticles through nanoprecipitation. In addition, conjugated polymers can be modified by encapsulation with silica. Currently, conjugated polymers are widely used in fluorescence imaging and sonodynamic therapy of tumors.

[0032] This invention synthesizes a near-infrared conjugated polymer and obtains a novel near-infrared imaging-guided sonodynamic reagent through nanotechnology. It can be used for fluorescence imaging-guided sonodynamic therapy, biomedical imaging, and can also be applied to drug loading and delivery. Attached Figure Description

[0033] Figure 1 Transmission electron microscope images of the material obtained in Example 1;

[0034] Figure 2 The absorption spectrum of the material obtained in Example 1;

[0035] Figure 3 The fluorescence emission spectrum of the material obtained in Example 1;

[0036] Figure 4 The image shows the acoustic and dynamic effects of the material obtained in Example 1 after treatment with glutathione.

[0037] Figure 5 The tumor cell inhibition effect of the materials obtained in Examples 1 and 3 after treatment with glutathione;

[0038] Figure 6 Near-infrared imaging images of tumors from the material obtained in Example 1;

[0039] Figure 7 The image shows the acoustic and dynamic effects of the material obtained in Example 2.

[0040] Figure 8 The image shows the acoustic and dynamic effects of the material obtained in Example 3 after treatment with glutathione.

[0041] Figure 9 The image shows the acoustic and dynamic effects of the material obtained in Example 4. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0043] Please see Figures 1 to 9 As shown, an activatable acoustic-dynamic nanomaterial based on a conjugated polymer and its preparation method include the following steps:

[0044] Step 1: Synthesis of Conjugated Polymers

[0045] Step 1.1 Weigh 72.8 mg (0.1 mmol) of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithiophenecyclopentadiene, the same molar amount of 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, and 10 mg of tetra(triphenylphosphine)palladium, add them to a reaction tube, add 8 mL of toluene under nitrogen protection, and react in an oil bath at 100 °C for 24 h;

[0046] Step 1.2 The reaction product from Step 1.1 was precipitated with a large amount of methanol. After complete precipitation, the product was collected by filtration and dried at 50-60℃ to obtain the conjugated polymer solid, hereinafter referred to as PCPDTBT-NO2.

[0047] Step 2: Water-soluble modification of conjugated polymers

[0048] Prepare a 1 mg / mL PCPDTBT-NO2 chloroform solution, mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10, emulsify by sonication, and remove the chloroform by heating at 60°C to obtain an aqueous solution of the corresponding conjugated polymer.

[0049] Step 3 can activate the preparation of acoustic-dynamic nanomaterials.

[0050] The solution obtained in step 2 was mixed with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and 2 mol / L sodium hydroxide aqueous solution in a volume ratio of 1:9:0.03:0.05:0.2:0.06. After reacting at 70°C for about 40 min, triethoxysilane-modified polyethylene glycol was added and the reaction was continued for 3 h. After purification by ultrafiltration, water-soluble biodegradable mesoporous silica-encapsulated PCPDTBT-NO2 nanoparticles were obtained.

[0051] The generation of reactive oxygen species under ultrasound was investigated using diphenylbenzofuran (DPBF) as a probe. The acoustic-dynamic effect of the material was assessed based on the change in absorbance at 417 nm. The specific testing procedure involved mixing DPBF with an aqueous solution of the sample, measuring the absorption of DPBF using a UV-Vis spectrophotometer, and then transmitting the solution through ultrasound (1 MHz, 1.5 W / cm²). 2 After treatment, its absorption spectrum was tested immediately, and the generation of reactive oxygen species was characterized by the decrease in absorbance of DPBF at 417 nm.

[0052] The specific implementation method is as follows:

[0053] Example 1:

[0054] 1) Weigh 72.8 mg (0.1 mmol) of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithiophenecyclopentadiene, the same molar amount of 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, and 10 mg of tetra(triphenylphosphine)palladium, add them to a reaction tube, add 8 mL of toluene under nitrogen protection, and react in an oil bath at 100 °C for 24 h;

[0055] 2) The reaction product was precipitated with methanol. After complete precipitation, the product was collected by filtration and dried at 50-60℃ to obtain a dark blue conjugated polymer.

[0056] 3) Prepare a 1 mg / mL PCPDTBT-NO2 chloroform solution and mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10. After ultrasonic emulsification, remove the chloroform by heating at 60℃ to obtain the corresponding aqueous solution. Mix the obtained solution with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and 2 mol / L sodium hydroxide aqueous solution at a volume ratio of 1:9:0.03:0.05:0.2:0.06. After reacting at 70℃ for about 40 min, add triethoxysilane-modified polyethylene glycol and continue reacting for 3 h. After ultrafiltration purification, obtain degradable water-soluble PCPDTBT-NO2 mesoporous silica nanoparticles. Characterize them using transmission electron microscopy and measure their absorption and fluorescence emission spectra.

[0057] 4) Add 10mM glutathione to the material and place it in a 37℃ oven to simulate the degradation process of the material in the tumor microenvironment. Detect its photodynamic and acoustic effects every day.

[0058] Experimental results show that, Figure 1 As shown, the material has an average particle size of approximately 20 nm, exhibiting uniform particle size and good dispersibility. Figure 2 As shown, the material has two absorption peaks near 420 nm and 650 nm; Figure 3 As shown, under 635nm light excitation, the conjugated polymer exhibits strong fluorescence at 950nm, which extends to 1200nm, reaching the near-infrared II region, and can be used for near-infrared II fluorescence imaging. Figure 4 As shown, at a power of 1.5W / cm 2 Under conditions of 1 minute of ultrasonication, the material showed almost no ability to degrade diphenylbenzofuran (DPBF), but it could degrade DPBF under 635 nm laser irradiation. Compared with Example 2, after coating with silica, the photodynamic effects of the conjugated polymer did not change significantly, while the acoustic dynamic effects were significantly suppressed. This may be related to the fact that the cavitation bubbles generated by ultrasound could not contact the conjugated polymer. Figure 4As shown, after GSH treatment, the mesoporous silica gradually degrades, and the acoustic dynamic properties of the material are significantly improved. Figure 5 As shown, cell viability experiments also demonstrated that the material, after GSH treatment, could effectively inhibit tumor cell growth under ultrasound. Figure 6 As shown, after the material is injected into the tail vein, near-infrared fluorescence imaging reveals that the material can be efficiently enriched in the tumor site of mice, and can be used for optical imaging and sonodynamic therapy of tumors.

[0059] Example 2:

[0060] 1) Prepare a 1 mg / mL PCPDTBT-NO2 chloroform solution, mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10, emulsify it by sonication, remove the chloroform under heating at 60°C to obtain the corresponding aqueous solution, without coating it with a silica shell, and directly test its acoustic and photodynamic properties under the same conditions as in Example 1.

[0061] Experimental results show that, Figure 7 As shown, the conjugated polymer exhibits good acoustic dynamics, and the material also shows good near-infrared II fluorescence and photodynamics under illumination.

[0062] Example 3:

[0063] 1) Prepare a 1 mg / mL PCPDTBT-NO2 chloroform solution, mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10, emulsify it by sonication, and remove the chloroform by heating at 60℃ to obtain the corresponding aqueous solution;

[0064] 2) The obtained solution was mixed with water, tetraethoxysilane, ethyl acetate, and 2 mol / L sodium hydroxide aqueous solution in a volume ratio of 1:9:0.04:0.2:0.06. After reacting at 70℃ for about 40 min, triethoxysilane-modified polyethylene glycol was added and the reaction was continued for 3 h. After purification by ultrafiltration, water-soluble PCPDTBT-NO2 mesoporous silica nanoparticles were obtained.

[0065] 4) Add 10mM glutathione to the material and place it in a 37℃ oven to simulate the degradation process of the material in the tumor microenvironment. Detect its photodynamic and acoustic effects every day.

[0066] Experimental results show that the fluorescence and photodynamic effects of this material are comparable to those of Examples 1 and 2, while its acoustic dynamic effects are very poor, similar to those of Example 1. Figure 8 As shown, because the silica encapsulated in this material is not degradable by GSH, it did not exhibit any activatable acoustic dynamic effects. Figure 5As shown, the material was not significantly inhibited by ultrasound before and after GSH treatment.

[0067] Example 4:

[0068] 1) Prepare a 1 mg / mL PCPDTBT (non-nitro-substituted conjugated polymer, purchased from Sigma-Aldrich) chloroform solution, mix it with a 20 mg / mL CTAB aqueous solution at a volume ratio of 1:10, ultrasonically emulsify it, and remove the chloroform under heating at 60°C to obtain the corresponding aqueous solution. Without coating with a silica shell, its acoustic and dynamic properties are directly tested under the same conditions as in Example 1.

[0069] Experimental results show that, Figure 9 As shown, the aqueous solution of this conjugated polymer has almost no acoustic dynamic effect, indicating that nitro substitution plays an important role in the acoustic dynamic properties of the material, which may be related to the electron-withdrawing properties and hydrophilicity of nitro groups.

[0070] In summary, this invention develops a novel conjugated polymer for near-infrared II imaging-guided ultrasound-dynamic therapy. This conjugated polymer itself possesses excellent fluorescence and acoustic-dynamic therapeutic properties. However, after encapsulation with mesoporous silica, the fluorescence properties are almost unaffected, while the acoustic-dynamic properties are significantly suppressed. This may be related to the silica layer hindering the contact between the conjugated polymer and the ultrasound cavitation bubble. After incorporating degradable disulfide bonds into the mesoporous silica, the material can be gradually degraded by the high concentration of glutathione (GSH) at the tumor site, activating its acoustic-dynamic therapeutic properties. This helps reduce damage to normal tissues and improves the specificity of tumor acoustic-dynamic therapy.

[0071] This invention is simple to operate, and the synthesized novel copolymer composite material has strong stability and good biocompatibility. It has near-infrared fluorescence and photodynamic therapy effects in the range of 700-1200 nm, and can be degraded by high concentrations of glutathione in tumors. The singlet oxygen generated under ultrasound gradually increases as degradation proceeds, which can be used for near-infrared imaging-guided activated sonodynamic therapy. Furthermore, the mesoporous silica encapsulation can also be used for drug loading and delivery.

[0072] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. The understanding of this specification should be based on those skilled in the art. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still make modifications or equivalent substitutions to the present invention. All technical solutions and improvements that do not depart from the spirit and scope of the present invention should be covered within the scope of the claims of the present invention.

Claims

1. A method for preparing activatable acoustic-dynamic nanomaterials based on conjugated polymers, characterized in that, Includes the following steps: 2,6-Di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienrocyclopentadiene, 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole, and tetra(triphenylphosphine)palladium were added to a reaction tube, and toluene was added under nitrogen protection. The tube was then placed in an oil bath and reacted at 100 °C for 8–48 h. The reaction product was precipitated with methanol, filtered, collected, and dried to obtain the conjugated polymer solid PCPDTBT-NO2. The molar ratio of 2,6-di(trimethyltin)-4,4-di(2-ethylhexyl)-dithienrocyclopentadiene and 4,7-dibromo-5,6-dinitrobenzo[c][1,2,5]thiadiazole was 1:

1. Prepare a PCPDTBT-NO2 chloroform solution, mix it with an aqueous CTAB solution, emulsify it with ultrasound, and remove the chloroform to obtain an aqueous solution of the conjugated polymer; An aqueous solution of the conjugated polymer was mixed with water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and an aqueous solution of sodium hydroxide. The mixture was reacted at 70 °C for 15–60 min. Triethoxysilane-modified polyethylene glycol was then added to continue the reaction. After purification by ultrafiltration, an activatable sonodynamic nanomaterial based on the conjugated polymer was obtained. The volume ratio of the aqueous solution of the conjugated polymer to water, bis-[3-(triethoxysilyl)propyl]-disulfide, tetraethoxysilane, ethyl acetate, and an aqueous solution of sodium hydroxide was 1:9:0.03:0.05:0.2:0.

06.

2. The method for preparing activatable acoustic-dynamic nanomaterials based on conjugated polymers according to claim 1, characterized in that, The reaction product was precipitated with methanol, filtered and collected, and dried at 50-60 °C to obtain the conjugated polymer solid PCPDTBT-NO2.

3. The method for preparing activatable acoustic-dynamic nanomaterials based on conjugated polymers according to claim 1, characterized in that, The concentration of PCPDTBT-NO2 in the PCPDTBT-NO2 chloroform solution is 1 mg / mL; the concentration of CTAB in the CTAB aqueous solution is 20 mg / mL; and the volume ratio of PCPDTBT-NO2 chloroform solution to CTAB aqueous solution is 1:8~12.

4. The method for preparing activatable acoustic-dynamic nanomaterials based on conjugated polymers according to claim 1, characterized in that, A PCPDTBT-NO2 chloroform solution was prepared, mixed with an aqueous CTAB solution, and ultrasonically emulsified. The chloroform was then removed by heating at 60 °C to obtain an aqueous solution of the conjugated polymer.

5. An activatable acoustic-dynamic nanomaterial based on a conjugated polymer, characterized in that, Prepared by the method described in any one of claims 1 to 4.

6. The application of the conjugated polymer-based activated acoustic dynamic nanomaterial as described in claim 5 in the preparation of near-infrared II luminescent acoustic sensitizer materials.

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