A rapid quantitative dried blood spot sampler

Abstract

The application discloses a kind of quick quantitative dry blood spot sampler, including test tube, test tube plug, blood collection tube, blood absorption filter paper;The application is designed into integrated, continuity, closed operation structure by quantitative blood suction and blood spot formation, after automatic quantitative blood suction is carried out by blood collection tube, the end of blood collection tube contacted with blood is inverted upward, since the upper end of blood collection tube is contacted with the lower end surface of blood absorption filter paper, so blood will flow downward under the action of gravity and is automatically absorbed by blood absorption filter paper to form blood spot, the desiccant preloaded in test tube plug can absorb moisture in blood to rapidly complete the drying of blood spot, so that the quantitative blood suction of blood collection tube and the formation and drying of blood spot of blood absorption filter paper become a coherent, closed step, the quickness and convenience of sampling are improved, the risk of contamination in the process of manual transfer of blood suctioned by blood collection tube and blood spot drying is avoided, and the efficiency of dry blood spot collection and preparation is greatly improved.

Description

Technical Field

[0001] This invention relates to a rapid quantitative dried blood spot sampler. Background Technology

[0002] Dried blood spot detection technology involves dripping a biological sample, typically whole blood, onto filter paper, drying and preserving it, and then extracting the analytes from the dried blood spot sample for analysis. This technology was initially used for the qualitative analysis of nucleic acid-based genetic material. Currently, the most widely used blood collection card for dried blood spot technology is cotton filter paper, made of uniform cotton fibers, which ensures even distribution of absorbed whole blood. Blood spots are typically collected using a microcapillary tube to draw, transfer, and drip the blood onto the blood collection card, then allow the blood on the card to dry, forming a dried blood spot. During detection, the traditional extraction process involves punching a fixed-size sample from the center of the blood collection card. The dried blood spot is placed in a suitable container, and the analyte is extracted with an appropriate extraction solution. Finally, it is detected by a detection instrument. However, the above method requires aspiration, transfer, and dropping onto the blood collection card, and the blood collection card is exposed to the outside air to dry. During the test, a circular piece of a fixed size needs to be punched from the center of the dried blood spot. This makes the process cumbersome, inefficient, and results in poor collection accuracy. Moreover, there is a risk of blood contamination when transferring blood to the blood collection card, and the blood spot is exposed to the outside air to dry, which also has the disadvantage of contamination. In addition, the blood on the blood collection card takes a long time to dry, resulting in low work efficiency. Summary of the Invention

[0003] In view of the shortcomings of the prior art, the present invention solves the problem of providing a rapid quantitative dried blood spot sampler that is fast, convenient, efficient, has a short drying time, and avoids blood sample contamination.

[0004] To solve the following problems, the technical solution adopted by the present invention is as follows: A rapid quantitative dried blood spot sampler includes a test tube, a test tube stopper, a blood collection tube, and absorbent filter paper. The upper end of the blood collection tube is movably connected to the lower end of the test tube stopper. The absorbent filter paper is installed between the test tube stopper and the blood collection tube. The upper end of the blood collection tube is in contact with the lower end of the absorbent filter paper. The test tube is inserted upward from the lower end of the blood collection tube, and the upper end of the test tube is movably and sealingly connected to the test tube stopper, while simultaneously sealing the blood collection tube and the absorbent filter paper inside the test tube.

[0005] Furthermore, the blood collection tube has an open end and a hollow interior; a connecting sleeve is provided around the upper end of the blood collection tube; the blood-absorbing filter paper is installed inside the connecting sleeve, and the middle of the lower end face of the blood-absorbing filter paper contacts the upper end of the blood collection tube; the connecting sleeve is movably connected to the lower end of the test tube stopper; the blood-absorbing filter paper is clamped and installed between the lower end of the connecting sleeve and the lower end of the test tube stopper.

[0006] Furthermore, the lower end of the test tube stopper is provided with a pressure strip; the pressure strip presses downward against the upper surface of the blood-absorbing filter paper.

[0007] Furthermore, the lower end of the test tube stopper is provided with an intermediate sleeve; the upper end of the intermediate sleeve is movably connected to the lower end of the test tube stopper; the upper end of the blood collection tube is movably connected to the lower end of the intermediate sleeve; the blood-absorbing filter paper is installed between the intermediate sleeve and the blood collection tube; and a desiccant is provided inside the test tube stopper.

[0008] Furthermore, the blood collection tube has an open end and a hollow interior; a connecting sleeve is provided around the upper end of the blood collection tube; the blood-absorbing filter paper is installed inside the connecting sleeve, and the middle of the lower end face of the blood-absorbing filter paper contacts the upper end of the blood collection tube; the connecting sleeve is movably connected to the lower end of the intermediate sleeve; the blood-absorbing filter paper is clamped and installed between the connecting sleeve and the intermediate sleeve.

[0009] Furthermore, the desiccant is in the form of flakes, granules, or powder.

[0010] Furthermore, a pressure strip is provided inside the lower end of the intermediate sleeve; the pressure strip presses downward against the upper surface of the blood-absorbing filter paper.

[0011] Furthermore, the pressure strip has a cross-shaped structure or a mesh structure.

[0012] Furthermore, the blood collection tube is made of hydrophilic plastic or glass material that automatically absorbs liquid.

[0013] Furthermore, reinforcing ribs are provided on both sides of the blood collection tube.

[0014] The beneficial effects of this invention are as described above: 1. This invention integrates the quantitative blood collection and blood spot formation into a continuous and closed operating structure. After the blood collection tube is automatically drawn, once it is full, the upper end of the tube is in contact with the lower end of the absorbent filter paper. By inverting the lower end of the tube upwards, the blood is automatically absorbed by the filter paper under gravity. This design integrates the blood collection tube's absorption and the filter paper's absorption to form a continuous and closed process, improving the speed and convenience of sampling, avoiding the risk of contamination during manual transfer of blood collected from the blood collection tube, and greatly improving sampling efficiency.

[0015] 2. In order to improve the drying properties, the present invention can fill the inside of the test tube stopper with a desiccant. The desiccant absorbs the moisture in the blood and actively dries the blood spots, thereby improving the speed of dried blood spot formation.

[0016] 3. In the detection process of this invention, it is only necessary to separate and disassemble the blood collection tube and the test tube stopper, put the dried blood spot formed by the blood-absorbing filter paper into the test tube of this invention, and then add the extraction solution directly into the test tube to extract and perform subsequent detection. The extraction of dried blood spot is convenient and quick, avoiding the traditional cumbersome hole-punching collection steps. At the same time, the test tube can be reused, reducing the cost of use. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the disassembled structure of the test tube and test tube stopper in Embodiment 1 of the present invention.

[0018] Figure 2 This is a schematic diagram showing the disassembled structure of the test tube stopper, blood-absorbing filter paper, blood collection tube, and connecting sleeve in Embodiment 1 of the present invention.

[0019] Figure 3 This is a schematic diagram of the connection structure in Embodiment 2 of the present invention.

[0020] Figure 4 This is a schematic diagram of the disassembled structure of the test tube and test tube stopper in Embodiment 2 of the present invention.

[0021] Figure 5 This is a schematic diagram showing the disassembled structure of the test tube stopper, desiccant, intermediate sleeve, blood-absorbing filter paper, connecting sleeve, and blood collection tube in Embodiment 2 of the present invention.

[0022] Figure 6 This is a top view of the structure of the pressure bar of the present invention.

[0023] The attached diagrams are labeled as follows: test tube—1, test tube stopper—2, blood collection tube—3, blood absorption filter paper—4, intermediate sleeve—5, lower pressure strip—6, desiccant—7, connecting sleeve—31, reinforcing rib—32. Detailed Implementation

[0024] The invention will be further described in detail above with reference to the accompanying drawings. Example 1

[0025] like Figure 1 , 2As shown in Figure 6, a rapid quantitative dried blood spot sampler includes a test tube 1, a test tube stopper 2, a blood collection tube 3, and absorbent filter paper 4. The test tube 1 is closed at the bottom and sides, and open at the top. The test tube stopper 2 is closed at the sides and top, and open at the bottom. The absorbent filter paper 4 is a filter paper sheet or a glass fiber sheet, which can automatically absorb blood to facilitate the formation of blood spots. The blood collection tube 3 is open at both ends and hollow inside. A connecting sleeve 31 is provided around the upper end of the blood collection tube 3. The longitudinal cross-section of the connecting sleeve 31 is U-shaped. The upper end of the blood collection tube 3 is inserted and fixed to the bottom center of the connecting sleeve 31, and the upper end of the blood collection tube 3 can be inserted and extended to the connecting sleeve 31. Inside the middle, the upper end of the blood collection tube 3 can also be flush with the lower inner side of the connecting sleeve 31. For stable clamping and installation of the blood-absorbing filter paper 4, this embodiment chooses the latter. The blood-absorbing filter paper 4 is installed inside the lower part of the connecting sleeve 31, and can be fitted against the lower inner side of the connecting sleeve 31, so that the lower side of the blood-absorbing filter paper 4 can directly adhere to the upper end of the blood collection tube 3. The connecting sleeve 31 is movably connected to the outer periphery of the lower end of the test tube stopper 2. Here, the connecting sleeve 31 can be directly sleeved onto the outer periphery of the lower end of the test tube stopper 2. A stepped annular surface can be provided on the outer periphery of the lower end of the test tube stopper 2 to facilitate the insertion of the connecting sleeve 31. The connection between the connecting sleeve 31 and the test tube stopper 2 needs to maintain a certain tightness, allowing the connecting sleeve 31 to be tightly fitted around the outer periphery of the test tube stopper 2. The blood-absorbing filter paper 4 is clamped and installed between the lower inner surface of the connecting sleeve 31 and the lower annular surface of the test tube stopper 2. The diameter of the blood-absorbing filter paper 4 should match the outer and inner diameters of the lower end of the test tube stopper 2; that is, the diameter of the blood-absorbing filter paper 4 should be greater than the inner diameter of the test tube stopper 2 but less than or equal to the outer diameter. Thus, when the liquid rises and fills the blood collection tube 3, inverting the blood collection tube 3 allows the blood to flow through gravity. The downward contact of the blood-absorbing filter paper 4 allows the liquid to be automatically absorbed by the filter paper 4. This is the most important design point of the present invention, realizing the integrated, continuous, and closed-loop quantitative absorption of liquid and blood spot formation. The test tube 1 is inserted upward from the lower end of the blood collection tube 3, and the upper end of the test tube 1 is connected to the test tube stopper 2 in a closed and movable manner. At the same time, the blood collection tube 3 and the blood-absorbing filter paper 4 are sealed inside the test tube 1. Here, an annular stepped surface can be provided around the upper end of the test tube stopper 2 to facilitate the insertion of the test tube 1. Alternatively, a threaded annular surface can be provided around the upper end of the test tube stopper 2 to allow the test tube 1 to be screwed on. The connection structure can be selected preferentially. In this embodiment, the former connection method is selected.To ensure stable liquid adsorption, a pressure strip 6 is provided inside the lower end of the test tube stopper 2. The lower end face of the pressure strip 6 is flush with the lower circumferential surface of the test tube stopper 2. The blood-absorbing filter paper 4 is installed against the lower end face of the pressure strip 6. The pressure strip 6 is preferably a cross-shaped structure or a mesh structure. Here, the lower circumferential surface of the test tube stopper 2 and the pressure strip 6 together press against the upper end face of the blood-absorbing filter paper 4. The pressure strip 6 is provided to press against the middle of the blood-absorbing filter paper 4, ensuring stable contact between the lower side of the blood-absorbing filter paper 4 and the upper end of the blood collection tube 3, preventing the connecting sleeve 31 from being blocked. The downward and upward compression of the lower end and the lower annular surface of the test tube stopper 2 causes the middle of the blood-absorbing filter paper 4 to bulge upward, creating a separation gap between the middle of the blood-absorbing filter paper 4 and the upper end of the blood collection tube 3. This prevents the blood-absorbing filter paper 4 from successfully and completely absorbing blood. In this embodiment, the blood collection tube 3 is made of hydrophilic plastic or glass material that automatically absorbs liquid, allowing for automatic blood absorption. Reinforcing ribs 32 are provided on both sides of the blood collection tube 3 to strengthen it and ensure its robustness and stability. Example 2

[0026] This embodiment refers to Embodiment 1, except that, in order to facilitate the addition of desiccant 7 into the test tube stopper 2 and the storage of desiccant 7, an intermediate sleeve 5 is provided at the lower end of the test tube stopper 2; as Figure 3 , 4As shown in Figures 5 and 6, the lower end of the test tube stopper 2 is provided with an intermediate sleeve 5, which has an open structure at both the upper and lower ends and is hollow inside. The upper end of the intermediate sleeve 5 is movably connected to the lower end of the test tube stopper 2. This connection can be made by external thread, internal thread, or a flexible and tight insertion structure, as long as the airtight connection is guaranteed. The upper end of the blood collection tube 3 is provided with a connecting sleeve 31 around its perimeter. The connection method between the connecting sleeve 31 and the blood collection tube 3 can refer to Example 1. The blood-absorbing filter paper 4 is fitted and installed on the lower inner side of the connecting sleeve 31. The connecting sleeve 31 is movably connected to the lower end of the intermediate sleeve 5. The connecting sleeve 31 can be fitted onto the outer perimeter of the lower end of the intermediate sleeve 5. The blood-absorbing filter paper 4 is clamped and installed between the lower inner side of the connecting sleeve 31 and the lower annular surface of the intermediate sleeve 5. The diameter of 4 is greater than the inner diameter of the intermediate sleeve 5 but less than or equal to the outer diameter of the intermediate sleeve 5; the connecting sleeve 31 can be elastically fastened and fitted around the lower end of the intermediate sleeve 5; the liquid is collected through the blood collection tube 3 as in Example 1, and after being filled, it is inverted so that the blood automatically moves down to the blood-absorbing filter paper 4 under the action of gravity, and the blood-absorbing filter paper 4 automatically absorbs the blood to form blood spots; the inside of the test tube stopper 2 is equipped with a desiccant 7, which can be in the form of flakes, granules, or powder. The test tube stopper 2 can be removed from the upper end of the intermediate sleeve 5, and then the desiccant 7 is filled into the test tube stopper 2 before it is installed on the upper end of the intermediate sleeve 5. In this way, when the test tube 1 is sealed with the test tube stopper 2, the blood spots can be dried by the desiccant 7, which accelerates the formation of dried blood spots. In this embodiment, the lower end of the intermediate sleeve 5 may be provided with a pressure strip 6. The pressure strip 6 is a cross-shaped structure or a mesh structure. The setting of the pressure strip 6 can ensure that the upper end of the blood collection tube 3 is in stable contact with the lower side of the blood absorption filter paper 4. In order to avoid the blood absorption filter paper 4 being squeezed and pressed by the lower side of the connecting sleeve 31 and the lower end annular surface of the intermediate sleeve 5, causing the blood absorption filter paper 4 to bulge upward and thus creating a gap between the upper end of the blood collection tube 3 and the blood absorption filter paper 4, it is necessary to use the pressure strip 6 with a cross-shaped structure or a mesh structure to abut and support the middle of the blood absorption filter paper 4, so as to ensure that the upper end of the blood collection tube 3 is in stable contact with the lower side of the blood absorption filter paper 4. The lower end annular surface of the intermediate sleeve 5 and the lower end surface of the pressure strip 6 jointly press against the upper side of the blood absorption filter paper 4.

[0027] like Figure 3 , 4As shown in Figures 5 and 6, the sampling and extraction operation steps of this embodiment 2 are as follows: The test tube stopper 2 is pulled out from the top of the test tube 1, causing the blood collection tube 3 to detach from the test tube 1. The blood collection tube 3 is then positioned horizontally or at an angle, with its lower end contacting the blood to be collected. The blood is automatically drawn into the blood collection tube 3 until it is completely filled. The lower end of the blood collection tube 3 is then removed from the blood collection tube, and the entire blood collection tube 3 is inverted. Due to gravity, the blood in the blood collection tube 3 flows downwards into the blood-absorbing filter paper 4. The blood-absorbing filter paper 4 quickly absorbs the blood, forming blood spots. Finally, the blood collection tube is... Test tube 1 is reconnected to test tube stopper 2 for sampling and preservation. The present invention features an integrated and continuous structure for quantitative blood sampling and blood spot formation, and the entire process is carried out in a closed manner. The blood spot is dried by the desiccant inside test tube stopper 2. When the blood spot needs to be tested after drying, test tube stopper 2 is pulled out from the top of test tube 1 again, and then connecting sleeve 31 is pulled out from the bottom of test tube stopper 2. The connecting sleeve 31 is gently squeezed to allow the dried blood spot inside the connecting sleeve 31 to be sent into test tube 1 of the present invention. Finally, extraction solution is directly added to test tube 1 for extraction and subsequent testing.

[0028] This invention integrates the quantitative blood collection and blood spot formation into a continuous, closed-loop operating structure. After the blood collection tube 3 automatically collects blood by contacting it horizontally or at an angle, and the entire tube 3 is filled with blood, the upper end of the tube 3 contacts the lower end of the absorbent filter paper. By inverting the lower end of the tube 3 upwards, the blood inside is automatically absorbed by the absorbent filter paper 4 under gravity. This integrates the quantitative blood collection from the blood collection tube 3 with the formation and drying of the blood spot on the absorbent filter paper 4 into a continuous, closed process. This improves the speed and convenience of sampling, avoids the risk of contamination during manual transfer of the blood collected from the blood collection tube 3, and greatly improves sampling efficiency. To enhance drying, a desiccant can be filled inside the test tube stopper. The desiccant absorbs moisture from the blood, actively drying the blood spot and improving the speed of dried blood spot formation. Furthermore, this invention uses a closed drying process, preventing blood spot contamination. During testing, the present invention only requires separating and disassembling the blood collection tube 3 and the test tube stopper 2, sending the dried blood spot formed by the blood-absorbing filter paper 4 into the test tube 1 of the present invention, and then directly adding the extraction solution to the test tube 1 to extract and perform subsequent testing. The extraction of dried blood spot is convenient and quick, avoiding the traditional cumbersome drilling and collection steps. At the same time, the test tube 1 of the present invention can be reused, reducing the cost of use.

Claims

1. A rapid quantitative dried blood spot sampler, characterized in that, The device includes a test tube (1), a test tube stopper (2), a blood collection tube (3), and blood-absorbing filter paper (4); the upper end of the blood collection tube (3) is movably connected to the lower end of the test tube stopper (2); the blood-absorbing filter paper (4) is installed between the test tube stopper (2) and the blood collection tube (3); the upper end of the blood collection tube (3) is in contact with the lower end of the blood-absorbing filter paper (4); the test tube (1) is inserted upward from the lower end of the blood collection tube (3), so that the upper end of the test tube (1) is movably sealed with the test tube stopper (2). The blood collection tube (3) and the blood-absorbing filter paper (4) are connected and sealed inside the test tube (1); the lower end of the test tube stopper (2) is provided with an intermediate sleeve (5); the upper end of the intermediate sleeve (5) is movably connected to the lower end of the test tube stopper (2); the upper end of the blood collection tube (3) is movably connected to the lower end of the intermediate sleeve (5); the blood-absorbing filter paper (4) is installed between the intermediate sleeve (5) and the blood collection tube (3); the inside of the test tube stopper (2) is provided with a desiccant (7).

2. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The blood collection tube (3) has an open end and a hollow interior; the upper end of the blood collection tube (3) is provided with a connecting sleeve (31) around its perimeter; the blood-absorbing filter paper (4) is installed inside the connecting sleeve (31), and the lower end face of the blood-absorbing filter paper (4) is in contact with the upper end of the blood collection tube (3); the connecting sleeve (31) is movably connected to the lower end of the test tube stopper (2); the blood-absorbing filter paper (4) is clamped and installed between the connecting sleeve (31) and the lower end of the test tube stopper (2).

3. The rapid quantitative dried blood spot sampler according to claim 2, characterized in that, The lower end of the test tube stopper (2) is provided with a pressure strip (6); the pressure strip (6) presses down against the upper surface of the blood-absorbing filter paper (4).

4. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The blood collection tube (3) has an open end and a hollow interior; the upper end of the blood collection tube (3) is provided with a connecting sleeve (31) around its perimeter; the blood-absorbing filter paper (4) is installed inside the connecting sleeve (31), and the lower end face of the blood-absorbing filter paper (4) is in contact with the upper end of the blood collection tube (3); the connecting sleeve (31) is movably connected to the lower end of the intermediate sleeve (5); the blood-absorbing filter paper (4) is clamped and installed between the connecting sleeve (31) and the intermediate sleeve (5).

5. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The desiccant (7) is a sheet-like, granular, or powdered desiccant (7).

6. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The lower end of the intermediate sleeve (5) is provided with a pressure strip (6); the pressure strip (6) presses down against the upper surface of the blood-absorbing filter paper (4).

7. The rapid quantitative dried blood spot sampler according to claim 3 or 6, characterized in that, The pressure strip (6) has a cross-shaped structure or a mesh structure.

8. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The blood collection tube (3) is made of hydrophilic plastic or glass material that automatically absorbs liquid.

9. The rapid quantitative dried blood spot sampler according to claim 1, characterized in that, The blood collection tube (3) is provided with reinforcing ribs (32) on both sides.

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