Preparation method and application of carbon-based antibacterial catalyst loaded with organic photovoltaic material

Abstract

The application discloses a preparation method and application of a carbon-based antibacterial catalyst loaded with organic photovoltaic materials, and the method comprises the following operation steps: A, crushing carbon materials to 3000-5000 meshes to obtain carbon-based materials; B, mixing organic photovoltaic materials and chloroform according to the following weight ratio: 0.01-0.015 g of the organic photovoltaic materials: 10-15 ml of chloroform, wherein the organic photovoltaic materials are PTQ10:PC71BM:ITIC-Th; C, uniformly coating the mixed solution of step B on the carbon-based materials of step A, and the dosage of the mixed solution and the carbon-based materials is 1:5000-10000 in weight ratio, and the carbon-based antibacterial catalyst loaded with organic photovoltaic materials is obtained after drying. The antibacterial catalyst obtained by the method can remove more than 99.9% of escherichia coli and staphylococcus aureus, has excellent sterilization capacity, environmental protection characteristics and non-invasiveness, is low in cost, can be repeatedly used, can be applied to the fields of fruit and vegetable preservation, food processing and food packaging, prolongs the shelf life of food, and is suitable for large-scale production.

Description

Technical Field

[0001] This invention belongs to the field of photocatalysis technology, specifically a method for preparing and applying a carbon-based supported organic photovoltaic material antibacterial catalyst. Background Technology

[0002] In recent years, various diseases caused by foodborne pathogens have become one of the most prominent health and safety issues globally. Numerous outbreaks of foodborne illnesses are related to microbial contamination in food, representing a pressing problem in food safety. Food sterilization and antimicrobial technologies can be divided into thermal processing and non-thermal processing. The former can damage or even destroy the nutrients and natural flavor of food, while the latter includes methods such as low-temperature freeze-drying, refrigeration, vacuum impregnation, and high-pressure pulse drying. Although these methods are environmentally friendly and maintain food quality, they require highly sophisticated equipment and energy, consuming significant resources. Therefore, finding and developing a safe, energy-efficient, efficient, economical, and environmentally friendly sterilization method is the best way to achieve sustainable development and is of great significance. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide a method for preparing and applying a carbon-based supported organic photovoltaic material antibacterial catalyst. This method has excellent bactericidal ability, environmental protection characteristics and non-invasiveness.

[0004] The present invention solves the above-mentioned technical problems by means of the following technical solution:

[0005] This invention discloses a method for preparing a carbon-based supported organic photovoltaic material antibacterial catalyst, comprising the following steps:

[0006] A. Crush carbon materials to 3000-5000 mesh to obtain carbon-based materials;

[0007] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01-0.015g organic photovoltaic material: 10-15ml chloroform to obtain a mixed solution. The organic photovoltaic material is PTQ10:PC71BM:ITIC-Th.

[0008] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000 to 10000. After drying, a carbon-based supported organic photovoltaic material antibacterial catalyst is obtained.

[0009] In step A, the carbon material is placed in a blender and pulverized for 30-60 seconds.

[0010] In step B, when the organic photovoltaic material PTQ10:PC71BM:ITIC-Th is mixed with chloroform, stirring is used at a speed of 500 r / min for 15 min.

[0011] In step B, the organic photovoltaic material PTQ10:PC71BM:ITIC-Th is prepared by mixing PTQ10, PC71BM, and ITIC-Th in a weight ratio of 1:1:1.

[0012] In step C, drying refers to drying in a constant temperature drying oven at 60°C for 24 hours.

[0013] The carbon-based supported organic photovoltaic material antibacterial catalyst obtained by the method of this invention can be used for the sterilization of harmful microorganisms. The operation is as follows: the carbon-based supported organic photovoltaic material antibacterial catalyst is placed in a solution containing harmful microorganism Escherichia coli (E. coli) or a solution containing harmful microorganism Staphylococcus aureus (S. aureus). A xenon lamp is used as the light source, supplemented with a filter. After the adsorption in the dark reaches equilibrium, the light is irradiated. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined by plate counting method. The kill rate is calculated until the sterilization purpose is achieved.

[0014] The xenon lamp has a power of 500W and a wavelength range of 420–760nm.

[0015] The concentration of both bacterial solutions was 10. 8 CFU / mL, the dosage of antibacterial catalyst is 1-5 mg of antibacterial catalyst per 1 ml of bacterial solution.

[0016] The carbon-based supported organic photovoltaic material antibacterial catalyst obtained by the method of this invention can be used for closed food preservation and antibacterial treatment. The operation is as follows: the carbon-based supported organic photovoltaic material antibacterial catalyst is placed in a transparent porous film package and sealed, and placed near the food to be preserved. The amount of the carbon-based supported photocatalytic bactericide is 0.1-0.5g of antibacterial catalyst per package of food to be preserved, and the weight of each package of food to be preserved is 1kg.

[0017] The preserved food includes fruits, vegetables, or meat products.

[0018] The photocatalytic technology employed in this invention is an ideal energy utilization technology. This technology oxidizes almost all organic pollutants by generating reactive oxygen species, without producing harmful substances. Compared to existing technologies, photocatalysis can deeply oxidize organic matter at room temperature, decomposing it into CO2 and H2O, and also imparts excellent sterilization capabilities to the air through its catalytic effect. By using photocatalysis to treat the gases in fruit and vegetable storage spaces, the ethylene released by the fruits and vegetables during storage is decomposed, reducing the ethylene content in the storage space, inhibiting over-ripening of the fruits and vegetables, and achieving the purpose of preservation.

[0019] The method of the present invention has the following beneficial effects:

[0020] (1) The carbon-based supported organic photovoltaic material antibacterial catalyst obtained in this invention can remove more than 99.9% of Escherichia coli and Staphylococcus aureus, and can be reused.

[0021] (2) Based on its own photocatalytic performance, the method of the present invention generates electron-hole pairs when irradiated by light, which react with water and oxygen in the air to generate hydroxyl radicals and superoxide radicals, which attack bacteria and outer cells, penetrate the cell membrane of bacteria and destroy the cell membrane structure, thereby completely killing bacteria. This process can achieve the purpose of highly efficient inactivation of bacteria without the aid of any external chemical substances, and has excellent bactericidal ability, environmental protection characteristics and non-invasiveness.

[0022] (3) The photocatalyst of the present invention has excellent antibacterial properties and can be applied in fields such as fruits and vegetables, food processing, and food packaging that come into direct contact with people. Moreover, the preparation method is simple, low-cost, and environmentally friendly, and can be industrialized, which has great promotional value.

[0023] (3) The matrix material used in this invention is a natural carbon-based material, which has many excellent properties such as being green, having high porosity, and having a large specific surface area. It is also reusable and biodegradable, achieving true green friendliness and environmental pollution-free. Detailed Implementation

[0024] The technical solution of the present invention will be further illustrated below with reference to specific embodiments, but the embodiments do not limit the present invention in any way.

[0025] Example 1

[0026] The preparation and application of the carbon-based supported organic photovoltaic material antibacterial catalyst of the present invention includes the following steps:

[0027] A. Put 10g of carbon material into a high-speed blender and pulverize for 50 seconds to obtain 5000 mesh carbon-based material.

[0028] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 15ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0029] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0030] D. Divide the carbon-based supported organic photovoltaic antibacterial catalyst obtained in step C into two parts, A and B. Part A is placed in a solution containing the harmful microorganism *Escherichia coli* (E. coli), and part B is placed in a solution containing the harmful microorganism *Staphylococcus aureus* (S. aureus). Both are placed in separate reaction vessels, each using a 500W xenon lamp as the light source, supplemented with a filter. Irradiation begins after dark-state adsorption reaches equilibrium, lasting 0–60 minutes. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined using the plate count method to calculate the kill rate. The wavelength range of the xenon lamp is 420–760 nm; the concentration of both microbial solutions is 10. 8 The amount of antibacterial catalyst used in the carbon-based supported organic photovoltaic material is 1.0 mg per 1 mL of bacterial solution.

[0031] The antibacterial effect of this embodiment is as follows: As can be seen from Table 1, after 45 minutes of light exposure, the kill rate of the two bacteria reached 99.9%, demonstrating an antibacterial effect.

[0032] Table 1. Sterilization rate of Example 1

[0033]

[0034] Example 2

[0035] The preparation and application of the carbon-based supported organic photovoltaic material antibacterial catalyst of the present invention includes the following steps:

[0036] A. Put 10g of carbon material into a high-speed blender and crush it for 40 seconds to obtain 3000 mesh carbon-based material.

[0037] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.015g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 12ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0038] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0039] D. Divide the carbon-based supported organic photovoltaic antibacterial catalyst obtained in step C into two parts, A and B. Part A is placed in a solution containing the harmful microorganism *Escherichia coli* (E. coli), and part B is placed in a solution containing the harmful microorganism *Staphylococcus aureus* (S. aureus). Both are placed in separate reaction vessels, each using a 500W xenon lamp as the light source, supplemented with a filter. Irradiation begins after dark-state adsorption reaches equilibrium, lasting 0–60 minutes. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined using the plate count method to calculate the kill rate. The wavelength range of the xenon lamp is 420–760 nm; the concentration of both microbial solutions is 10. 8 The amount of CFU / mL for the antibacterial catalyst of the carbon-based supported organic photovoltaic material is 2.0 mg of antibacterial catalyst per 1 mL of bacterial solution.

[0040] The antibacterial effect of this embodiment is as follows: As can be seen from Table 2, after 30 minutes of light exposure, the kill rate of the two bacteria reached 99.9%, demonstrating an antibacterial effect.

[0041] Table 2. Sterilization rate of Example 2

[0042]

[0043] Example 3

[0044] The preparation and application of the carbon-based supported organic photovoltaic material antibacterial catalyst of the present invention includes the following steps:

[0045] A. Put 10g of carbon material into a high-speed blender and pulverize for 50 seconds to obtain 5000 mesh carbon-based material.

[0046] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 15ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0047] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:10000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0048] D. Divide the carbon-based supported organic photovoltaic antibacterial catalyst obtained in step C into two parts, A and B. Part A is placed in a solution containing the harmful microorganism *Escherichia coli* (E. coli), and part B is placed in a solution containing the harmful microorganism *Staphylococcus aureus* (S. aureus). Both are placed in separate reaction vessels, each using a 500W xenon lamp as the light source, supplemented with a filter. Irradiation begins after dark-state adsorption reaches equilibrium, lasting 0–60 minutes. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined using the plate count method to calculate the kill rate. The wavelength range of the xenon lamp is 420–760 nm; the concentration of both microbial solutions is 10. 8 The amount of antibacterial catalyst used in the carbon-based supported organic photovoltaic material is 1.0 mg per 1 mL of bacterial solution.

[0049] The antibacterial effect of this embodiment is as follows: As can be seen from Table 3, after 60 minutes of light exposure, the kill rate of the two bacteria reached 99.9%, demonstrating an antibacterial effect.

[0050] Table 3. Sterilization rate of Example 3

[0051]

[0052] Example 4

[0053] The preparation and application of the carbon-based supported organic photovoltaic material antibacterial catalyst of the present invention includes the following steps:

[0054] A. Put 10g of carbon material into a high-speed blender and crush it for 30 seconds to obtain 5000 mesh carbon-based material.

[0055] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 10ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0056] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0057] D. Divide the carbon-based supported organic photovoltaic antibacterial catalyst obtained in step C into two parts, A and B. Part A is placed in a solution containing the harmful microorganism *Escherichia coli* (E. coli), and part B is placed in a solution containing the harmful microorganism *Staphylococcus aureus* (S. aureus). Both are placed in separate reaction vessels, each using a 500W xenon lamp as the light source, supplemented with a filter. Irradiation begins after dark-state adsorption reaches equilibrium, lasting 0–60 minutes. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined using the plate count method to calculate the kill rate. The wavelength range of the xenon lamp is 420–760 nm; the concentration of both microbial solutions is 10. 8 The amount of antibacterial catalyst used in the carbon-based supported organic photovoltaic material is 3.0 mg per 1 mL of bacterial solution.

[0058] The antibacterial effect of this embodiment is as follows: As can be seen from Table 4, after 20 minutes of light exposure, the kill rate of the two bacteria reached 99.9%, demonstrating an antibacterial effect.

[0059] Table 4. Sterilization rate of Example 4

[0060]

[0061] Example 5

[0062] The preparation and application of the carbon-based supported organic photovoltaic material antibacterial catalyst of the present invention includes the following steps:

[0063] A. Put 10g of carbon material into a high-speed blender and pulverize for 45 seconds to obtain 5000 mesh carbon-based material.

[0064] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.015g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 10ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0065] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0066] D. Divide the carbon-based supported organic photovoltaic antibacterial catalyst obtained in step C into two parts, A and B. Part A is placed in a solution containing the harmful microorganism *Escherichia coli* (E. coli), and part B is placed in a solution containing the harmful microorganism *Staphylococcus aureus* (S. aureus). Both are placed in separate reaction vessels, each using a 500W xenon lamp as the light source, supplemented with a filter. Irradiation begins after dark-state adsorption reaches equilibrium, lasting 0–60 minutes. Samples are taken every 15 minutes, and the concentration of surviving bacteria is determined using the plate count method to calculate the kill rate. The wavelength range of the xenon lamp is 420–760 nm; the concentration of both microbial solutions is 10. 8 The dosage of the carbon-based supported organic photovoltaic material antibacterial catalyst is 5.0 mg of antibacterial catalyst per 1 mL of bacterial solution.

[0067] The antibacterial effect of this embodiment is as follows: Table 5 shows that after 10 minutes of light exposure, the kill rate of the two bacteria reached 99.9%, demonstrating an antibacterial effect.

[0068] Table 5. Sterilization rate of Example 5

[0069]

[0070] Example 6

[0071] The application of the carbon-based supported organic photovoltaic material antibacterial catalyst in strawberry preservation according to this invention includes the following steps:

[0072] A. Put 10g of carbon material into a high-speed blender and pulverize for 50 seconds to obtain 5000 mesh carbon-based material.

[0073] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 13ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0074] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:7000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0075] D. The carbon-based supported organic photovoltaic material antibacterial catalyst obtained in step C was sealed in a transparent porous film package to prepare a preservative. The sealed antibacterial catalyst was placed in an open 20cm square transparent box containing 1kg of strawberries and placed at 37℃ for a period of time. The appearance and other indicators of the strawberries were observed. The amount of the photocatalytic antibacterial agent used was 0.1g. Under the same temperature conditions, the control group only placed 10 strawberries in an open 20cm square transparent box.

[0076] The antibacterial effect in this embodiment is as follows: In the control group without the antibacterial catalyst of this invention, strawberries began to rot on day 1 and were completely rotten on day 2. In the experimental group with the antibacterial catalyst of this invention, slight rot began to occur on day 4 and was completely rotten on day 5, demonstrating excellent antibacterial effect.

[0077] Example 7

[0078] The application of the carbon-based supported organic photovoltaic material antibacterial catalyst in the preservation of raw beef, as described in this invention, includes the following steps:

[0079] A. Put 10g of carbon material into a high-speed blender and pulverize for 50 seconds to obtain 5000 mesh carbon-based material.

[0080] B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01g of organic photovoltaic material PTQ10:PC71BM:ITIC-Th : 13ml of chloroform to obtain a mixed solution. The organic photovoltaic material is composed of PTQ10, PC71BM, and ITIC-Th mixed in a weight ratio of 1:1:1. When mixing the organic photovoltaic material with chloroform, use a magnetic stirrer to stir at a speed of 500 rpm for 15 minutes.

[0081] C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:7000. Then, it is dried in a constant temperature drying oven at 60°C for 24 hours to obtain a carbon-based supported organic photovoltaic material antibacterial catalyst.

[0082] D. The carbon-based supported organic photovoltaic material antibacterial catalyst obtained in step C was sealed in a transparent porous film package to prepare a preservative. The sealed organic photovoltaic material was placed in an uncovered square transparent box with a side length of 20cm containing 1kg of raw beef, and placed at 37℃ for a period of time. The appearance, odor, and other indicators of the raw beef were observed. The amount of the antibacterial catalyst used was 0.5g. Under the same temperature conditions, the control group only placed the same size of raw beef in an uncovered 20cm square transparent box.

[0083] The antibacterial effect of this embodiment is as follows: In the control group without the antibacterial catalyst of this invention, raw beef began to spoil at 14 hours, producing an irritating odor, and was completely spoiled at 24 hours. In the experimental group with the antibacterial catalyst of this invention, slight spoilage began at 28 hours, and was completely spoiled at 40 hours, demonstrating excellent antibacterial effect.

Claims

1. A method for preparing a carbon-based supported organic photovoltaic material antibacterial catalyst, characterized in that, The following steps are included: A. Crush carbon materials to 3000-5000 mesh to obtain carbon-based materials; B. Mix the organic photovoltaic material with chloroform in the following weight ratio: 0.01-0.015g organic photovoltaic material: 10-15ml chloroform. Mix by stirring at a speed of 500r / min for 15min to obtain a mixed solution. The organic photovoltaic material is PTQ10:PC71BM:ITIC-Th; the organic photovoltaic material PTQ10:PC71BM:ITIC-Th is prepared by mixing PTQ10, PC71BM, and ITIC-Th in a weight ratio of 1:1:

1. C. The mixed solution from step B is uniformly coated onto the carbon-based material from step A. The weight ratio of the mixed solution to the carbon-based material is 1:5000 to 10000. The mixture is dried in a constant temperature drying oven at 60°C for 24 hours. After drying, a carbon-based supported organic photovoltaic material antibacterial catalyst is obtained.

2. The method for preparing the carbon-based supported organic photovoltaic material antibacterial catalyst according to claim 1, characterized in that, In step A, the carbon material is placed in a blender and pulverized for 30-60 seconds.

3. The application of the carbon-based supported organic photovoltaic material antibacterial catalyst obtained by the preparation method according to claim 1 or 2, characterized in that, The carbon-based supported organic photovoltaic material antibacterial catalyst was used for the sterilization of harmful microorganisms. The operation method is as follows: The carbon-based supported organic photovoltaic material antibacterial catalyst was placed in a solution containing harmful microorganism Escherichia coli or Staphylococcus aureus. A xenon lamp was used as the light source, supplemented with a filter. After the adsorption in the dark reached equilibrium, the light was started. Samples were taken every 15 minutes, and the concentration of surviving bacteria was determined by plate counting method. The kill rate was calculated until the sterilization purpose was achieved.

4. The application of the carbon-based supported organic photovoltaic material antibacterial catalyst according to claim 3, characterized in that, The xenon lamp has a power of 500W and a wavelength range of 420–760nm.

5. The application of the carbon-based supported organic photovoltaic material antibacterial catalyst according to claim 3, characterized in that, The concentration of both bacterial solutions was 10. 8 CFU / mL, the dosage of antibacterial catalyst is 1-5 mg of antibacterial catalyst per 1 ml of bacterial solution.

6. The application of the carbon-based supported organic photovoltaic material antibacterial catalyst obtained by the preparation method according to claim 1 or 2, characterized in that, The carbon-based supported organic photovoltaic material antibacterial catalyst is used for closed food preservation and antibacterial purposes. The operation is as follows: the carbon-based supported organic photovoltaic material antibacterial catalyst is placed in a transparent porous film package and sealed. It is then placed near the food to be preserved. The amount of the carbon-based supported photocatalytic bactericide is 0.1-0.5g of antibacterial catalyst per package of food to be preserved. The weight of each package of food to be preserved is 1kg.

7. The application of the carbon-based supported organic photovoltaic material antibacterial catalyst according to claim 6, characterized in that, The preserved food includes fruits, vegetables, or meat products.

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