244 site-based s-adenosylmethionine synthetase mutant and application thereof

By performing site-directed mutagenesis and soluble tagging on the S-adenosylmethionine synthase of Guyparkeria sp. XI15, the problems of low enzyme catalytic efficiency and high production cost were solved, achieving efficient and high-purity S-adenosylmethionine production and reducing production costs.

CN117551629BActive Publication Date: 2026-06-12SHANDONG UNIV OF TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV OF TECH
Filing Date
2022-11-11
Publication Date
2026-06-12

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Abstract

The application belongs to the technical field of bioengineering and particularly relates to a S-adenosylmethionine synthetase mutant based on a 244 site and application thereof. Guyparkeria sp The wild strain of the S-adenosylmethionine synthetase mutant of the application is derived from XI15, and a mutant with a mutation of core amino acids related to enzyme catalytic activity is obtained through site-directed mutation; only one of the following mutations exists in the 1th to 390th amino acid sequence of SEQ ID NO: 1: D22S; G123D; D244A; K251I; G266S; A307S; the amino acid sequence is one of SEQ ID NO: 3-8; the S-adenosylmethionine synthetase mutant of the application realizes the application in S-adenosylmethionine biosynthesis, so that the yield of S-adenosylmethionine can reach 25.7 g / L, and the purity of process products can reach 89.7%.
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