A fully orthogonal system for protein synthesis in bacterial cells

Tethered ribosomes with ligated subunits address the limitations of bipartite ribosomes by enabling orthogonal translation systems for sequence-controlled polymers and novel properties, including non-natural amino acid incorporation and antibiotic resistance.

JP7872551B2Active Publication Date: 2026-06-10NORTHWESTERN UNIV +1
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
NORTHWESTERN UNIV
Filing Date
2021-03-24
Publication Date
2026-06-10

AI Technical Summary

⚠Technical Problem

Existing ribosomes are limited by their bipartite nature, preventing the development of fully orthogonal systems capable of evolving new functions without interfering with native translation, as the free exchange of subunits hinders the creation of specialized ribosomes with novel properties.

⚗Method used

Modified ribosomes with tethered subunits, where the small and large subunits are ligated by a polynucleotide sequence, allowing for the translation of sequence-controlled polymers and enabling the use of native mRNA while maintaining functional separation of subunits.

🎯Benefits of technology

The tethered ribosomes facilitate the translation of sequence-controlled polymers, including non-natural amino acids, and allow for the development of orthogonal translation systems with improved properties, such as antibiotic resistance and gain-of-function mutations.

✦ Generated by Eureka AI based on patent content.

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Abstract

Disclosed are engineered polynucleotides, engineered ribosomes comprising the engineered polynucleotides, engineered cells and systems comprising the engineered polynucleotides and ribosomes, and methods of making and using the engineered polynucleotides, engineered ribosomes, engineered cells and systems. The engineered polynucleotides, engineered ribosomes, and engineered cells can be used to prepare sequence-defined polymers and to select mutant ribosomes that are capable of incorporating non-canonical amino acids into polymers.
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