Increased production of acetyl-coenzyme a and derived products in yeast
By genetically modifying yeast cells to express heterologous phosphoketolase and optimizing non-oxidative glycolysis pathways, acetyl-CoA production is enhanced, improving the yield of fermentation products such as acetone, isopropanol, and ethanol.
WO2026133258A1PCT designated stage Publication Date: 2026-06-25DANSTAR FERMENT AG
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- DANSTAR FERMENT AG
- Filing Date
- 2025-12-18
- Publication Date
- 2026-06-25
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Figure IB2025063192_25062026_PF_FP_ABST
Abstract
The present disclosure describes a recombinant yeast host cell having a first engineered metabolic pathway to convert fructose-6-phosphate (F-6-P) into acetyl-coenzyme A. The first engineered metabolic pathway comprises: a genetic modification for expressing a heterologous phosphoketolase capable of converting fructose-6-phosphate into erythrose-4-phosphate, and at least one genetic modification for decreasing native 6- phosphofructo-2-kinase activity classified under Enzyme Commission No. 2.7.1.105. Optionally, the recombinant yeast host cell comprises a second engineered metabolic pathway for convert acetyl-coA into a fermentation product. The present disclosure further describes a process for making a fermentation product. The process comprises contacting the recombinant yeast host cell described herein with a carbohydrate source under a condition allowing the conversion of at least a part of the carbohydrate into a fermentation product.
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