MiniSTR fluorescent multiplex amplification system and kit with 18 short tandem repeat sequences
The MiniSTR fluorescent multiplex amplification system and six-color fluorescent labeling kit have solved the problems of amplification of degraded and trace DNA samples, enabling efficient individual identification and paternity testing, and are suitable for rapid detection of various human samples.
Patent Information
- Application Number
- CN202311848427.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-29
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2043-12-29
AI Technical Summary
Existing STR technology has difficulty achieving effective amplification in degraded and trace DNA samples, leading to difficulties in individual identification and paternity testing, especially in complex cases where sample preservation is poor, resulting in DNA structural changes that lead to fragment loss and increased inhibitor concentrations.
A MiniSTR fluorescent multiplex amplification system was designed, containing 18 short tandem repeat sequence sites. Using six-color fluorescent labeling and an optimized PCR amplification kit, it can simultaneously amplify 17 autosomal STR sites and 1 sex site in a single tube. The amplification product is less than 300 bp, making it suitable for samples with high degradation or high inhibitor content.
It improves the success rate of typing trace amounts and degraded DNA, enhances individual identification rate, makes up for the shortcomings of traditional kits in typing degraded samples, shortens detection time and improves experimental efficiency, and is suitable for the detection of a variety of human samples.