Skin barrier repair composition containing prunus armeniaca extract and use thereof

Abstract

The present application relates to a skin barrier repair composition containing a Prinsepia uniflora extract and application thereof, and the skin barrier repair composition comprises the Prinsepia uniflora extract, the Physella fructus extract, the Origanum vulgare extract and the Portulaca oleracea extract. The Prinsepia uniflora extract, the Physella fructus extract, the Origanum vulgare extract and the Portulaca oleracea extract are combined creatively to form a brand-new composition mode, and the four are scientifically matched and synergized, the composition has excellent skin repair efficacy and anti-aging efficacy, and the specific performance is that the composition can promote the increase of the epidermal layer thickness, reduce the water loss rate of the damaged skin, accelerate the wound healing speed, and has a remarkable effect on promoting the expression of the repair-related genes and promoting the expression of the elastin and the collagen. In addition, the safety has been well verified, and the composition can be safely applied to related products.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a skin barrier repair composition containing extract of sea buckthorn fruit and its application, specifically to a skin barrier repair composition containing extract of sea buckthorn fruit and its application in the preparation of cosmetics. Background Technology

[0002] Aging, also known as aging, is an inevitable stage in the life process, and skin aging is the most obvious manifestation of this process. Skin aging not only manifests as wrinkles, sagging, and pigmentation, but also as a decrease in epidermal metabolism, a weakening of the epidermis's ability to repair damage, and a reduced barrier function.

[0003] As research into the physiological structure and mechanisms of the skin continues to expand, our understanding of the skin barrier is constantly growing. A healthy skin barrier is a crucial factor in maintaining healthy skin. The most common skin manifestation caused by a damaged skin barrier is sensitive skin. Furthermore, most skin diseases also involve skin barrier damage, such as acne, seborrheic dermatitis, melasma, rosacea, and steroid-induced dermatitis. Additionally, mesotherapy and laser treatments, which are most widely used in the medical aesthetics field, are also accompanied by skin barrier damage.

[0004] Human skin is generally considered to be a metabolic system, and the skin barrier is also a multi-dimensional and multi-faceted system that interacts and influences each other. From the outermost sebum film to the most important brick-and-mortar structure, to the water regulation mechanisms such as aquaporins and natural moisturizing factors, to the inflammation and immune regulation mechanisms, and finally to the activation and remodeling of the dermis, each layer has its own appropriate regulatory mechanism. If a problem occurs in any link, it will induce problems in other links, thus forming a vicious cycle.

[0005] There are many skin barrier repair products on the market, most of which focus on repairing the skin's "brick wall" structure and addressing aging in the dermis, but their targets are not comprehensive. Some products simply provide a non-irritating, moisturizing environment for the skin to repair itself, and these products often feel heavy and oily, failing to provide a comfortable user experience.

[0006] Some products illegally contain added hormones, causing new skin problems such as steroid-induced dermatitis. A significant number of products also contain harmful chemicals (such as irritating chemical preservatives), which not only irritate the skin and easily trigger allergic reactions like redness and swelling, but also damage the skin's barrier system and reduce its ability to retain moisture. As people's living standards improve, consumers have increasingly higher demands for cosmetics, requiring products to not only have skin-repairing effects but also to be natural, safe, healthy, and gentle.

[0007] Therefore, it is very meaningful to develop a safe, gentle product that can effectively repair the skin barrier. Summary of the Invention

[0008] To address the shortcomings of existing technologies, the present invention aims to provide a skin barrier repair composition containing *Rhizoma Cirsium japonicum* extract and its application, specifically providing a skin barrier repair composition containing *Rhizoma Cirsium japonicum* extract and its application in the preparation of cosmetics.

[0009] To achieve this objective, the present invention employs the following technical solution:

[0010] In a first aspect, the present invention provides a skin barrier repair composition containing *Pyracantha fortuneana* fruit extract, the skin barrier repair composition comprising: *Pyracantha fortuneana* fruit extract, *Phyllostachys edulis* calyx extract, oregano extract and purslane extract.

[0011] This invention creatively combines four plant extracts—*Rhizoma Cirsium japonicum*, *Caulis Phyllanthus*, *Radix Oregano*, and *Radix Portulacae*—to form a novel composition. These four extracts work synergistically to produce superior skin repair and anti-aging effects. Specifically, they promote increased epidermal thickness, reduce water loss from damaged skin, and accelerate wound healing. Furthermore, they significantly promote the expression of repair-related genes and the expression of elastin and collagen. In addition, their safety has been well-verified, making them safe for use in related products.

[0012] Preferably, the skin barrier repair composition comprises, by weight, 5-10 parts of *Prickly pear fruit* extract, 1-5 parts of *Prunus armeniaca* calyx extract, 1-5 parts of *Oregano* extract, and 1-5 parts of *Portulaca oleracea* extract.

[0013] Based on the potential synergistic relationship between the four plant extracts mentioned above, when they meet the specific mass ratios described above, the composition exhibits superior skin repair and anti-aging effects.

[0014] The weight parts of the prickly ash fruit extract can be 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.

[0015] The weight parts of the *Phyllostachys edulis* extract can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0016] The weight parts of the oregano extract can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0017] The weight parts of the purslane extract can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0018] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0019] In this invention, the extracts of *Prickly pear* fruit and *Prunus armeniaca* calyx can be made from commercially available raw materials, but products prepared by the specific method described below are preferred, as they have more prominent technical effects in skin barrier repair and anti-oxidation.

[0020] Preferably, the sea buckthorn fruit extract is prepared by a method comprising the following steps:

[0021] (S1) After mixing the raw material of the prickly pear fruit with water, microwave treatment is performed, and then the treated mixture is subjected to vacuum decompression reflux extraction to obtain the extract.

[0022] (S2) Filter the extract, concentrate the filtrate, and obtain a concentrated solution;

[0023] (S3) Mix the concentrated liquid with the alcohol solution and perform alcohol precipitation;

[0024] (S4) Filter the alcohol precipitation solution, dry the precipitate, and obtain the prickly pear fruit extract.

[0025] Preferably, the raw material of *Pteris vittata* is selected from any one or a combination of at least two of the following: *Pteris vittata* seed meal powder, *Pteris vittata* kernel, *Pteris vittata* fruit, *Pteris vittata* leaves, or *Pteris vittata* roots.

[0026] The raw material parts of the prickly pear fruit used in this invention are not limited, but prickly pear seed meal and prickly pear kernels are preferred. Prickly pear seed meal is the residue powder left after pressing prickly pear kernels for oil. This invention provides a new strategy for the secondary development of prickly pear seed meal, which can realize waste utilization and is more economical and environmentally friendly.

[0027] Preferably, the microwave processing conditions include: microwave power of 150-400W, such as 150W, 180W, 200W, 220W, 250W, 300W, 350W, 400W, etc.; and microwave time of 30-90min, such as 30min, 40min, 50min, 60min, 70min, 80min, 90min, etc.

[0028] The microwave-assisted treatment described above can further improve the extraction efficiency of the target components. Within the specific microwave treatment parameter range, it can balance extraction efficiency and the stability of the target components, thereby maximizing the technical effect of the final product.

[0029] Preferably, the vacuum degree of the vacuum decompression reflux extraction is 0.03-0.08 MPa, such as 0.03 MPa, 0.04 MPa, 0.05 MPa, 0.06 MPa, 0.07 MPa, 0.08 MPa, etc.

[0030] Preferably, the temperature of the vacuum decompression reflux extraction is 35-45℃, such as 35℃, 36℃, 37℃, 38℃, 39℃, 40℃, 41℃, 42℃, 43℃, 44℃, 45℃, etc.

[0031] Preferably, the filtrate is concentrated to 1 / 6 to 1 / 2 of its volume, for example, 1 / 6, 1 / 5, 1 / 4, 1 / 3, 1 / 2, etc.

[0032] Concentrating the filtrate to the specific range defined above allows for better coordination with subsequent alcohol precipitation. If the volume is further concentrated, the remedial effect of the alcohol precipitation product will be negatively affected.

[0033] Preferably, the alcohol solution is an anhydrous alcohol solution or an aqueous alcohol solution, and the alcohol concentration of the aqueous alcohol solution is 90-99%, such as 90%, 92%, 93%, 94%, 95%, 96%, 97%, 99%, etc.

[0034] Preferably, the final volume concentration of alcohol after mixing the alcohol solution and the concentrate is 80-90%, such as 80%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, etc.

[0035] Preferably, the alcohol is selected from ethanol, a combination of ethanol and butanediol, or a combination of ethanol, butanediol, and polyethylene glycol.

[0036] The alcohol used in the above-mentioned alcohol precipitation treatment can be ethanol alone, or a combination of ethanol and butanediol, or a combination of ethanol, butanediol and polyethylene glycol. The choice of alcohol type affects the alcohol precipitation effect to a certain extent. When butanediol or polyethylene glycol is further added to ethanol, the efficacy of the final product is further improved.

[0037] Preferably, the volume ratio of ethanol to butanediol is (1-4):1, for example, 1:1, 2:1, 3:1, 4:1, etc.

[0038] Preferably, the volume ratio of ethanol to butanediol to polyethylene glycol is (1-4):1:(0.05-0.2), wherein the specific values ​​in (0.05-0.2) can be selected from 0.05, 0.07, 0.1, 0.12, 0.14, 0.15, 0.2, etc.

[0039] Preferably, the number average molecular weight of the polyethylene glycol is 200-600, such as 200, 300, 400, 500, 600, etc.

[0040] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0041] Preferably, the calyx extract is prepared by a method comprising the following steps:

[0042] (T1) The raw material of *Phyllostachys pubescens* was mixed with a eutectic solvent and then extracted by heating in a water bath to obtain the extract;

[0043] (T2) The extract is concentrated under reduced pressure and dried to obtain the calyx extract.

[0044] The extract of *Phyllostachys pubescens* is creatively prepared using a eutectic solvent extraction method, which results in a product with better repair and anti-aging effects than the product obtained by the traditional ethanol reflux extraction method.

[0045] Preferably, the eutectic solvent is obtained by reacting a hydrogen bond acceptor with a hydrogen bond donor, wherein the hydrogen bond donor is citric acid and the hydrogen bond acceptor is betaine.

[0046] This invention has found that different combinations of hydrogen bond acceptors and hydrogen bond donors result in eutectic solvents with varying repair and anti-aging effects on the resulting *Phyllostachys edulis* extract. A preferred approach is to use citric acid as the hydrogen bond donor and betaine as the hydrogen bond acceptor.

[0047] Preferably, the reaction is carried out at 40-80°C until the system is a clear and transparent liquid.

[0048] Preferably, the ratio of the acid calyx raw material to the eutectic solvent is 1:(5-20)g / mL, for example, 1:5g / mL, 1:7g / mL, 1:8g / mL, 1:10g / mL, 1:12g / mL, 1:14g / mL, 1:16g / mL, 1:20g / mL, etc.

[0049] Preferably, the water bath heating extraction temperature is 45-65℃, such as 45℃, 50℃, 55℃, 60℃, 65℃, etc.; and the time is 30-90min, such as 30min, 40min, 50min, 60min, 70min, 80min, 90min, etc.

[0050] In a second aspect, the present invention relates to the use of the skin barrier repair composition according to the first aspect in the preparation of cosmetics.

[0051] Thirdly, the present invention provides a cosmetic product, the components of which, by mass percentage, comprise: 0.5-10% of the skin barrier repair composition described in the first aspect, 20-40% of a moisturizer, 5-30% of a polyol, and 50-95% of water.

[0052] The mass percentage of the skin barrier repair composition can be selected from 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc. The mass percentage of the moisturizer can be selected from 20%, 22%, 25%, 28%, 30%, 32%, 35%, 38%, 40%, etc. The mass percentage of the polyol can be selected from 5%, 8%, 10%, 12%, 15%, 20%, 22%, 25%, 30%, etc. The mass percentage of the water can be selected from 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, etc.

[0053] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0054] Preferably, the cosmetic components, by weight percentage, further include any one or a combination of at least two of the following: skin conditioning agent 1-10%, pH adjuster 0.1-2%, thickener 0.05-2%, solubilizer 0.02-1%, chelating agent 0.01-0.05%, fragrance 0.1-1%, and preservative 0.05-1%.

[0055] The mass percentage of the skin conditioning agent can be selected from 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc. The mass percentage of the pH adjuster can be selected from 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.5%, 2%, etc. The mass percentage of the thickener can be selected from 0.05%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.5%, 2%, etc. The mass percentage of the solubilizer can be selected from 0.02%, 0.05%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc. The mass percentage of the chelating agent can be selected from 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, etc. The mass percentage of the fragrance can be selected from 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc. The mass percentage of the preservative can be selected from 0.05%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc.

[0056] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0057] Preferably, the moisturizer includes any one or a combination of at least two of hyaluronic acid, sodium hyaluronate, sclerotium gum, trehalose, aloe vera, panthenol, and erythritol.

[0058] Preferably, the polyol includes any one or a combination of at least two of butanediol, pentanediol, propylene glycol, and glycerol.

[0059] Compared with the prior art, the present invention has the following beneficial effects:

[0060] This invention creatively combines four plant extracts—*Rhizoma Cirsium japonicum*, *Caulis Prunellae*, *Radix Oregano*, and *Radix Portulacae*—to form a novel composition. These four extracts work synergistically to produce superior skin repair and anti-aging effects. Specifically, they promote increased epidermal thickness, reduce water loss from damaged skin, accelerate wound healing, and significantly promote the expression of repair-related genes and elastin and collagen. Furthermore, their safety has been well-verified, allowing for safe application in related products. Attached Figure Description

[0061] Figure 1 This is a graph showing the PPAR-β expression levels in each group during an acute mouse skin injury model experiment.

[0062] Figure 2 This is a graph showing the LOR expression levels in each group during an acute mouse skin injury model experiment.

[0063] Figure 3 This is a graph showing the Claudin-5 expression levels in each group during an acute mouse skin injury model experiment.

[0064] Figure 4 This is a graph showing the SPT expression levels in each group during an acute mouse skin injury model experiment.

[0065] Figure 5 This is a graph showing the Eln1 expression levels in each group during the zebrafish elastin assay.

[0066] Figure 6 This is a graph showing the expression levels of col1a1 in each group during the zebrafish collagen assay. Detailed Implementation

[0067] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0068] Unless otherwise specified, the extracts of *Prickly pear* fruit, *Prunus armeniaca* calyx, *Oregano*, and *Portulaca oleracea* mentioned below are all from commercially available sources. Information on their commercial sources is as follows:

[0069] Prickly pear fruit extract: provided by Shanghai Betterness Health Technology Co., Ltd., batch number: 20230724;

[0070] Phyllanthus urinaria calyx extract: provided by Shanghai Betterness Health Technology Co., Ltd., batch number: 20230715;

[0071] Oregano extract: provided by Shanghai Jiyan Biomedical Development Co., Ltd., batch number: 20230703;

[0072] Purslane extract: provided by Shanghai Jiyan Biomedical Development Co., Ltd., batch number: 20230718.

[0073] Preparation Example 1-1

[0074] This preparation example provides a sea buckthorn fruit extract, the preparation method of which is as follows:

[0075] (1) Mix the seed meal powder of the Chinese prickly pear with water at a ratio of 1:10 g / mL and then microwave it. The microwave power is 250W and the microwave time is 60min. Then, the treated mixture is vacuum refluxed twice at a vacuum degree of 0.05MPa and a temperature of 40℃ for 90min each time. The two extracts are combined.

[0076] (2) Filter the extract and concentrate the filtrate under reduced pressure to 1 / 4 of the original volume to obtain the concentrate;

[0077] (3) Add 95% ethanol aqueous solution dropwise to the concentrate while stirring, and add it all within 30 minutes to make the final volume concentration of ethanol 85%.

[0078] (4) The alcohol precipitation solution was filtered through a filter cloth with a pore size of 200 mesh, the precipitate was freeze-dried, and the extract of the prickly pear fruit was obtained.

[0079] Preparation Examples 1-2

[0080] This preparation example provides a Chinese prickly pear fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that: in step (3), the 95% ethanol aqueous solution is replaced with the same concentration of butanediol aqueous solution, while other conditions remain unchanged.

[0081] Preparation Examples 1-3

[0082] This preparation example provides a Chinese prickly pear fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that: in step (3), the 95% ethanol aqueous solution is replaced with a mixed alcohol aqueous solution of the same concentration (the volume ratio of ethanol to butanediol is 2:1), and all other conditions remain unchanged.

[0083] Preparation Examples 1-4

[0084] This preparation example provides a Chinese prickly pear fruit extract, the preparation method of which differs from that of Preparation Example 1-1 only in that: in step (3), the 95% ethanol aqueous solution is replaced with a mixed alcohol aqueous solution of the same alcohol concentration (the volume ratio of ethanol and butanediol and polyethylene glycol 300 is 2:1:0.1), and all other conditions remain unchanged.

[0085] Preparation Example 2-1

[0086] This preparation example provides an extract of *Phyllostachys pubescens* calyx, prepared by the following method:

[0087] (1) After crushing the calyx of the physalis, it is mixed with a eutectic solvent at a material-to-liquid ratio of 1:10 g / mL and then extracted by water bath heating at 55°C for 60 min to obtain the extract; the eutectic solvent is citric acid and betaine in a molar ratio of 1:1 reacted at 60°C until the system is a clear and transparent liquid.

[0088] (2) The extract was concentrated under reduced pressure and the concentrate was freeze-dried to obtain the calyx extract.

[0089] Preparation Example 2-2

[0090] This preparation example provides an extract of *Phyllostachys edulis*, the only difference between which preparation method differs from that of Preparation Example 2-1: the eutectic solvent used is different, namely, glycolic acid and betaine in a molar ratio of 4:1 reacted at 60°C until the system is a clear and transparent liquid; all other conditions remain unchanged.

[0091] Preparation Examples 2-3

[0092] This preparation example provides an extract of *Phyllostachys edulis*, the only difference between which preparation method differs from that of Preparation Example 2-1: the eutectic solvent used is different, namely, citric acid and choline chloride in a molar ratio of 1:1 reacted at 60°C until the system is a clear and transparent liquid; all other conditions remain unchanged.

[0093] Preparation Examples 2-4

[0094] This preparation example provides an extract of *Phyllostachys pubescens* calyx, prepared by the following method:

[0095] (1) After crushing the calyx of the physalis, it was mixed with 60% ethanol aqueous solution at a material-to-liquid ratio of 1:10 g / mL and then heated and refluxed for 60 min to obtain the extract.

[0096] (2) The extract was concentrated under reduced pressure and the concentrate was freeze-dried to obtain the calyx extract.

[0097] Example 1

[0098] This embodiment provides a composition, which is obtained by uniformly mixing 8 parts by weight of *Cynanchum paniculatum* fruit extract, 4 parts by weight of *Prunus armeniaca* calyx extract, 4 parts by weight of oregano extract and 4 parts by weight of purslane extract.

[0099] Example 2

[0100] This embodiment provides a composition, which is obtained by uniformly mixing 9 parts by weight of *Cynanchum paniculatum* fruit extract, 3 parts by weight of *Prunus armeniaca* calyx extract, 5 parts by weight of oregano extract and 3 parts by weight of purslane extract.

[0101] Example 3

[0102] This embodiment provides a composition, which is obtained by uniformly mixing 7 parts by weight of *Cynanchum paniculatum* fruit extract, 5 parts by weight of *Prunus armeniaca* calyx extract, 3 parts by weight of oregano extract and 5 parts by weight of purslane extract.

[0103] Examples 4-7

[0104] This embodiment provides four compositions, which differ from those in Example 1 in that commercially available sea buckthorn fruit extract is replaced in equal amounts with sea buckthorn fruit extracts prepared in Preparation Examples 1-1, 1-2, 1-3, and 1-4.

[0105] Examples 8-11

[0106] This embodiment provides four compositions, which differ from those in Example 1 in that: commercially available physalis extract is replaced in equal amounts with physalis extracts prepared in Preparation Examples 2-1, 2-2, 2-3, and 2-4.

[0107] Comparative Example 1

[0108] This comparative example provides a composition that differs from Example 1 in that it lacks the extract of *Cynanchum paniculatum* fruit and is obtained by uniformly mixing 6.7 parts by weight of *Prunus armeniaca* calyx extract, 6.7 parts by weight of oregano extract and 6.6 parts by weight of purslane extract.

[0109] Comparative Example 2

[0110] This comparative example provides a composition that differs from Example 1 in that it lacks the calyx extract of *Pyracantha fortuneana* and is obtained by uniformly mixing 10 parts by weight of *Pyracantha fortuneana* fruit extract, 5 parts by weight of oregano extract and 5 parts by weight of purslane extract.

[0111] Comparative Example 3

[0112] This comparative example provides a composition that differs from Example 1 in that it lacks oregano extract and is obtained by uniformly mixing 10 parts by weight of sea buckthorn fruit extract, 5 parts by weight of purslane calyx extract and 5 parts by weight of purslane extract.

[0113] Comparative Example 4

[0114] This comparative example provides a composition that differs from Example 1 in that it lacks purslane extract and is obtained by uniformly mixing 10 parts by weight of *Prickly pear fruit* extract, 5 parts by weight of *Prunus armeniaca* calyx extract, and 5 parts by weight of oregano extract.

[0115] Application Example 1

[0116] This application example provides a cosmetic composition formulated as follows: 5% of the composition obtained in Example 1, 3% sodium hyaluronate, 3% trehalose, 5% panthenol, 3% propylene glycol, 10% glycerin, and the balance being water. All components are homogenized to obtain the final product.

[0117] Application Example 2-11

[0118] This application example provides ten cosmetic compositions, the only difference between which are the same as those in Application Example 1. The compositions prepared in Example 1 are replaced in equal amounts with the compositions prepared in Examples 2-11, while all other conditions remain unchanged.

[0119] Compare and contrast examples 1-4

[0120] This application example provides four cosmetic compositions whose formulations differ from Application Example 1 only in that the compositions obtained in Example 1 are replaced in equal amounts with the compositions obtained in Comparative Examples 1-4, while all other conditions remain unchanged.

[0121] Test Example 1

[0122] 3D Skin Epidermal Layer Thickness Change Evaluation Experiment:

[0123] Preparation of test sample solution: The cosmetic compositions prepared in Application Examples 1-11 and Comparative Application Examples 1-4 were diluted with physiological saline solution to a concentration of 0.5%.

[0124] Experimental procedure: A commercially available 3D skin model-artificial epidermal detection kit (Jinan Pansheng Biotechnology Co., Ltd.) was purchased for the experiment. By adding the cosmetic composition and leaving it for 48 hours, if the epidermal layer thickness increased, it indicates that the cosmetic composition has a barrier repair effect. The blank control group was the same amount of physiological saline solution added.

[0125] The experimental results are shown in Table 1.

[0126] Table 1

[0127]

[0128]

[0129] As shown in Table 1, the skin barrier repair composition involved in this invention has a good effect on promoting the increase of epidermal thickness. The comparison results between Application Example 1 and Comparative Application Examples 1-4 show that the extracts of *Cynanchum paniculatum*, *Prunus armeniaca*, *Oregano*, and *Portulaca oleracea* have a synergistic effect in promoting the increase of epidermal thickness.

[0130] Test Example 2

[0131] Acute mouse skin injury model experiment:

[0132] Preparation of test sample solution: The cosmetic compositions prepared in Application Examples 1-11 and Comparative Application Examples 1-4 were diluted with physiological saline solution to a concentration of 0.5%.

[0133] An acute mouse skin injury model was established using the acetone-ether method. Specifically, male SPF-grade BALB / c mice (16-18g) purchased after passing quarantine were housed in standard polycarbonate cages under a 12-hour light / dark cycle, with free access to food and water. All procedures were performed in accordance with relevant ethical requirements for laboratory animals. A total of three groups were established: a normal control group, a model group, and experimental groups 1-15, with 6 mice in each group.

[0134] Hair removal: On the first day of the experiment, the hair on the back of the animals was shaved off using a hair removal device, covering an area of ​​approximately 2×2cm. 2 If, during the experiment, animal hair growth becomes too large to interfere with sample administration and observation, shaving is necessary. The hair removal process should be gentle to avoid damaging the skin.

[0135] Sample loading: From day 2 to day 7 of the experiment, medication was administered to mice after hair removal. Model group: Administered medication twice daily, at 9:00 AM and 5:00 PM. Each time, a cotton pad soaked in an equal volume mixture of acetone and ether was applied to the shaved area on the back of the mouse's neck for 15 seconds, followed by a cotton pad soaked in distilled water for another 15 seconds. This was repeated for 6 consecutive days. Normal control group: Compared to the model group, the acetone and ether mixture was replaced with physiological saline; all other parameters remained the same. Experimental groups 1-15: Compared to the model group, after modeling, distilled water was replaced with the experimental sample solution for each group; all other parameters remained the same.

[0136] Testing: On the eighth day of the experiment, the TEWL (transepidermal water loss) value of each group of mice was measured using an epidermal water loss meter. Compared with the model group, a smaller TEWL value indicates a better repair effect.

[0137] The transepidermal water loss rate data for each group are shown in Table 2.

[0138] Table 2

[0139]

[0140]

[0141] As shown in Table 2, the skin barrier repair composition involved in this invention has a good effect on reducing transepidermal water loss. The comparison results between Application Example 1 and Comparative Application Examples 1-4 show that the extracts of *Cynanchum paniculatum*, *Phyllanthus urinaria*, *Oregano*, and *Portulaca oleracea* have a synergistic effect in reducing transepidermal water loss.

[0142] mRNA expression level assay for repair-related genes:

[0143] Experimental procedure: After the above tests were completed, all mice were euthanized by cervical dislocation, and the skin on the back of the mice after hair removal and drug administration was collected for relevant tests.

[0144] The mRNA expression levels of keratinocyte proliferation and differentiation-related protein (PPAR-β), keratinization mantle-related protein (LOR), tight junction protein (Claudin-5), and lipid synthase (SPT) in each group of mice are as follows: Figure 1 , Figure 2 , Figure 3 , Figure 4 As shown.

[0145] Depend on Figure 1-4 It can be seen that, compared with the model group, after applying the skin barrier repair composition provided by this invention, the expression levels of the four protein mRNAs related to repair in the mouse skin of experimental groups 1-15 were increased to varying degrees, indicating that it has a good repair effect. Although the absence of any one of the skin barrier repair compositions also showed a certain degree of significance compared with the model group, the final repair effect would be greatly reduced. This indicates that the extracts of *Prickly ash* fruit, *Prunus armeniaca* calyx, *Oregano* extract, and *Portulaca oleracea* extract have a synergistic effect on the above-mentioned efficacy.

[0146] Test Example 3

[0147] Zebrafish elastin and collagen expression level assay:

[0148] Preparation of test sample solution: The cosmetic compositions prepared in Application Examples 1-11 and Comparative Application Examples 1-4 were diluted with zebrafish embryo culture medium solution to a concentration of 0.2%.

[0149] Experimental objective: To test the expression of elastin (Eln1) and type I collagen (col1a1) genes in zebrafish, compare the changes in the relative expression levels of elastin and type I collagen genes in the experimental group and the normal control group, and calculate the expression promotion rate of elastin and type I collagen genes to evaluate the firming and anti-wrinkle effects of the product.

[0150] Test method:

[0151] Experimental groups 1-15: Healthy zebrafish 6 days after fertilization were selected and transferred to 24-well plates. Each well contained 12 zebrafish and 2.5 mL of experimental sample solution. Each group of samples had 3 parallel wells. The only difference between the normal control group and the experimental group was that the 2.5 mL of experimental sample solution was replaced with 2.5 mL of fish embryo culture medium.

[0152] Culture process: The normal control group and the experimental group were cultured in a 28℃ incubator for 24 h. Twelve zebrafish from each well were collected into 1.5 mL test tubes, the solution was removed, 0.5 mL of RNAlater solution (Invitrogen; AM7020) was added, and the tubes were frozen for storage.

[0153] RNA extraction, cDNA synthesis, and real-time RT-PCR processes are detailed in: Zhang Hanjing, et al. A fibronectin nanoparticle formulation with tissue repair, anti-wrinkle, and soothing effects, its preparation method and application: 202310010878 [P].

[0154] Data and Result Calculation: Real-time PCR data were obtained. The relative expression levels of each gene (Eln1, col1a1) were calculated as test results. For ease of comparison and statistics, the relative expression level of each gene (Eln1, col1a1) in the normal control group zebrafish was set to 1.

[0155] The relative expression levels of elastin (Eln1) and type I collagen (col1a1) genes in each group are as follows: Figure 5 , Figure 6 As shown. By Figure 5-6 It is known that the skin barrier repair composition involved in this invention has varying degrees of enhancement effects on the elastin (Eln1) and type I collagen gene (col1a1) of zebrafish, indicating that the skin barrier repair composition not only has a good repair effect, but also has excellent firming and anti-wrinkle effects. However, although the absence of any one of the skin barrier repair composition results in a certain degree of significance compared to the normal control group, the final firming and anti-wrinkle effects will be greatly reduced. This indicates that the extracts of *Pyracantha fortuneana*, *Phyllostachys edulis*, *Oregano*, and *Portulaca oleracea* have a synergistic effect on the above-mentioned effects.

[0156] Test Example 4

[0157] Safety tests:

[0158] According to the "Cosmetic Safety Technical Specifications" (2015 edition), acute eye irritation test, repeated skin irritation test, skin allergy test, skin phototoxicity test, cell phototoxicity test, and hormone, microbial, and heavy metal tests were conducted on Examples 1, 4, and 8, respectively; pesticide residue tests were conducted according to GB / T39665-2020; the safety of the compositions obtained in the examples was jointly evaluated. The results are shown in Table 3.

[0159] Table 3

[0160]

[0161] Therefore, the skin barrier repair composition involved in this invention is safe and non-irritating.

[0162] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0163] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0164] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A skin barrier repair composition containing extract of sea buckthorn fruit, characterized in that, The skin barrier repair composition comprises, by weight, 5-10 parts of *Pyracantha fortuneana* fruit extract, 1-5 parts of *Pyracantha fortuneana* calyx extract, 1-5 parts of *Oregano* extract, and 1-5 parts of *Portulaca oleracea* extract. The sea buckthorn fruit extract is prepared by a method comprising the following steps: (S1) After mixing the raw material of the prickly pear fruit with water, microwave treatment is performed, and then the treated mixture is subjected to vacuum decompression reflux extraction to obtain the extract. The conditions for microwave processing include: microwave power of 150-400W and microwave time of 30-90 min. The vacuum degree of the vacuum decompression reflux extraction is 0.03-0.08 MPa, and the temperature is 35-45℃; (S2) Filter the extract and concentrate the filtrate to 1 / 6-1 / 2 of its volume to obtain a concentrated solution; (S3) Mix the concentrated liquid with the alcohol solution for alcohol precipitation; (S4) Filter the alcohol precipitation solution, dry the precipitate, and obtain the prickly pear fruit extract; The calyx extract of *Phyllostachys edulis* is prepared by a method comprising the following steps: (T1) The raw material of *Phyllostachys edulis* was mixed with a eutectic solvent and then extracted by heating in a water bath to obtain the extract; The eutectic solvent is obtained by reacting a hydrogen bond acceptor with a hydrogen bond donor, wherein the hydrogen bond donor is citric acid and the hydrogen bond acceptor is betaine. (T2) The extract is concentrated under reduced pressure and dried to obtain the calyx extract.

2. The skin barrier repair composition according to claim 1, characterized in that, The raw material of *Cyprinus pubescens* is selected from any one or a combination of at least two of the following: *Cyprinus pubescens* seed meal, *Cyprinus pubescens* kernel, *Cyprinus pubescens* fruit, *Cyprinus pubescens* leaf, or *Cyprinus pubescens* root.

3. The skin barrier repair composition according to claim 1, characterized in that, The alcohol solution is an anhydrous alcohol solution or an aqueous alcohol solution, and the alcohol concentration of the aqueous alcohol solution is 90-99%.

4. The skin barrier repair composition according to claim 1, characterized in that, The final volume concentration of alcohol after mixing the alcohol solution and the concentrate is 80-90%.

5. The skin barrier repair composition according to claim 1, characterized in that, The alcohol is selected from ethanol, a combination of ethanol and butanediol, or a combination of ethanol, butanediol, and polyethylene glycol.

6. The skin barrier repair composition according to claim 5, characterized in that, The volume ratio of ethanol to butanediol is (1-4):

1.

7. The skin barrier repair composition according to claim 5, characterized in that, The volume ratio of ethanol to butanediol to polyethylene glycol is (1-4):1:(0.05-0.2).

8. The skin barrier repair composition according to claim 5, characterized in that, The number average molecular weight of the polyethylene glycol is 200-600.

9. The skin barrier repair composition according to claim 1, characterized in that, The reaction between the hydrogen bond acceptor and the hydrogen bond donor is carried out at 40-80°C until the system becomes a clear and transparent liquid.

10. The skin barrier repair composition according to claim 1, characterized in that, The ratio of the acid calyx raw material to the eutectic solvent is 1:(5-20) g / mL.

11. The skin barrier repair composition according to claim 1, characterized in that, The water bath heating extraction temperature is 45-65℃, and the time is 30-90 min.

12. The use of the skin barrier repair composition according to any one of claims 1-11 in the preparation of cosmetics.

13. A cosmetic product, characterized in that, The cosmetic composition comprises, by weight percentage: 0.5-10% of the skin barrier repair composition according to any one of claims 1-11, 20-40% of the moisturizer, 5-30% of the polyol, and 50-95% of the water.

14. The cosmetic product according to claim 13, characterized in that, The cosmetic components, by mass percentage, also include any one or a combination of at least two of the following: skin conditioning agent 1-10%, pH adjuster 0.1-2%, thickener 0.05-2%, solubilizer 0.02-1%, chelating agent 0.01-0.05%, fragrance 0.1-1%, and preservative 0.05-1%.

15. The cosmetic product according to claim 13, characterized in that, The moisturizer includes any one or a combination of at least two of the following: hyaluronic acid, sodium hyaluronate, sclerotium gum, trehalose, aloe vera, panthenol, and erythritol.

16. The cosmetic product according to claim 13, characterized in that, The polyols include any one or a combination of at least two of butanediol, pentanediol, propylene glycol, and glycerol.

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