Application of licorice-wolfberry-based carbon quantum dots in crop drip irrigation fertilizers
By synthesizing licorice-goji berry-based carbon quantum dots as drip irrigation fertilizer in a one-pot process, the problems of high cost and serious environmental pollution in existing technologies have been solved. This method enhances the salt tolerance of crops and is suitable for improving the yield and health of various crops under salt stress.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- TARIM UNIV
- Filing Date
- 2024-03-22
- Publication Date
- 2026-06-23
Smart Images
Figure CN118221460B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural production technology, and in particular to the application of licorice-goji berry-based carbon quantum dots as drip irrigation fertilizer to improve crop salt tolerance. Background Technology
[0002] Soil salinization is a global agricultural challenge. In Xinjiang, severe saline-alkali slopes significantly restrict local agricultural development. Various food crops, cash crops, and fruits and vegetables are widely cultivated in Xinjiang. Under salt stress, crop fresh weight and SPAD values decrease, leading to reduced crop yields and consequently lower agricultural output. Plant nanobiology induced by salinity is an emerging interdisciplinary field with unique approaches to improving crop salt tolerance. However, most existing nanomaterials capable of increasing crop fresh weight and SPAD values under salt stress are synthesized from industrially sourced raw materials, requiring rigorous purification and safety assessment processes. This undoubtedly increases the costs of technology transfer, promotion, and production.
[0003] The root system is the organ through which plants absorb water and nutrients, and it is also the first part of the plant to be affected by salt and alkali stress, making it most susceptible to damage and thus affecting wheat yield. Drip irrigation technology in Xinjiang is very mature, allowing for quick and even application of fertilizers while saving significant costs. However, most existing drip irrigation fertilizers are synthesized from industrially produced raw materials, causing considerable environmental pollution.
[0004] Therefore, finding a drip irrigation fertilizer made from nanomaterials to improve crop salt tolerance is of great significance for the development of sustainable agriculture. Summary of the Invention
[0005] In view of this, the present invention provides a method for preparing licorice-goji-based carbon quantum dots and their application in improving crop salt tolerance when used as drip irrigation fertilizer. The licorice-goji-based carbon quantum dots synthesized by the present invention through a one-pot method can effectively improve the salt tolerance of crops.
[0006] To achieve the above objectives, the present invention adopts the following technical solution:
[0007] The application of licorice-goji-based carbon quantum dots in crop drip irrigation fertilizer, wherein the licorice-goji-based carbon quantum dots are used as drip irrigation fertilizer to improve the salt tolerance of crops.
[0008] Preferably, the preparation method of the licorice-goji-based carbon quantum dots is as follows: Goji berry powder and licorice powder are mixed and then a solvent is added. The mixture is heated to react, cooled and separated, and the supernatant is collected and stored at 3-5℃ for later use.
[0009] Preferably, the mass ratio of the wolfberry powder to the licorice powder is 1:(0.5-2);
[0010] And / or the ratio of the sum of the masses of the wolfberry powder and licorice powder to the volume of the solvent is (10-30) mg: 1 ml;
[0011] And / or the solvent is distilled water.
[0012] Preferably, the heating reaction temperature is 150-180℃ and the reaction time is 4-8h.
[0013] Preferably, the separation method is centrifugal separation, wherein the centrifugation is performed at 2000-10000 rpm for 30-90 minutes.
[0014] Preferably, the application of licorice-goji-based carbon quantum dots in crop drip irrigation fertilizer involves dissolving and diluting the licorice-goji-based carbon quantum dots to obtain a nanomaterial solution, and then soaking the roots of crop seedlings in the nanomaterial solution.
[0015] Preferably, the concentration of the nanomaterial solution is 50-300 mg / L.
[0016] Preferably, the soaking time is 1-5 hours.
[0017] Preferably, after soaking, the crop seedlings are placed in the dark to adapt for 2-5 hours, and then subjected to salt stress with 50-500mM NaCl solution, and the changes in the crop seedlings are recorded.
[0018] Preferably, the crops include wheat, corn, rice, cotton, beans, and rapeseed.
[0019] This invention provides an application of licorice-goji berry-based carbon quantum dots in crop drip irrigation fertilizer. Compared with existing technologies, its advantages are as follows:
[0020] This invention uses wolfberry and licorice as the main raw materials to synthesize licorice-wolfberry-based carbon quantum dots through a one-pot hydrothermal method. The synthesis method is simple, and the synthesized solution can be used after simple separation or filtration. Furthermore, the application of licorice-wolfberry-based carbon quantum dots to the roots of crops can effectively increase the fresh weight and SPAD value of crops under salt stress. Attached Figure Description
[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0022] Figure 1 TEM images of the synthesized products of Example 1, Comparative Example 1, and Comparative Example 2. Detailed Implementation
[0023] The embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the embodiments, they are performed in accordance with the techniques or conditions described in the literature in the art or in accordance with the product instructions. Reagents or instruments used, unless otherwise specified, are all conventional products that can be obtained commercially.
[0024] In one aspect of this invention, an embodiment of the invention proposes a method for preparing licorice-goji-based carbon quantum dots as follows: Goji berries and licorice are pulverized, then the goji berry powder and licorice powder are mixed evenly, a solvent is added, the mixture is heated to react, cooled, and separated. The supernatant is collected to obtain a brown liquid with a sweet aroma, which is the licorice-goji-based carbon quantum dots. The licorice-goji-based carbon quantum dots are stored at 3-5℃ for later use. Salt-tolerant metabolites from licorice and goji berries can be extracted using a hydrothermal method to form nanomaterials.
[0025] In some embodiments of the present invention, the mass ratio of the wolfberry powder to the licorice powder is 1:(0.5-2), for example, it can be 1:0.5, 1:1, 1:1.5, 1:2, etc., preferably 1:1. The ratio of the sum of the mass of the wolfberry powder and the licorice powder to the volume of the solvent is (10-30) mg:1 ml, wherein the solvent is preferably distilled water, and each milliliter of distilled water can dissolve, for example, 10 mg, 20 mg, or 30 mg of the raw material powder. Preferably, the ratio of the sum of the mass of the wolfberry powder and the licorice powder to the volume of the solvent is 20 mg:1 ml.
[0026] Furthermore, the reaction temperature of the heating reaction is 150-180℃, for example, 150℃, 160℃, 170℃, 180℃, etc.; the reaction time is 4-8h, for example, 4h, 5h, 6h, 8h, etc.
[0027] Preferably, the separation method is centrifugal separation or filtration separation, preferably centrifugal separation, wherein the centrifugation is performed at 2000-10000 rpm for 30-90 min, preferably at 5000 rpm for 60 min, to obtain a brown supernatant with a sweet aroma.
[0028] In another aspect of the present invention, the present invention provides an application of licorice-goji-based carbon quantum dots as a drip irrigation fertilizer to improve the salt tolerance of crops. Specifically, the licorice-goji-based carbon quantum dots are dissolved and diluted to obtain a nanomaterial solution, and the root system of crop seedlings is soaked in the nanomaterial solution.
[0029] Furthermore, the concentration of the nanomaterial solution is 50-300 mg / L, preferably 100-200 mg / L, and more preferably 150 mg / L. When the concentration of the nanomaterial solution is 150 mg / L, the fresh weight and SPAD value of the crop seedlings are higher.
[0030] Furthermore, the time for soaking crop seedlings in nanomaterial solution is 1-5 hours, for example, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, etc. Soaking can improve the salt tolerance of crops.
[0031] Furthermore, after soaking, the crop seedlings were placed in the dark to adapt for 2-5 hours, and then subjected to salt stress using 50-500mM NaCl solution. Changes in the crop seedlings were recorded. By changing the concentration of NaCl solution, the salinity of the soil in which the crop was planted was simulated, and changes in the crop were observed.
[0032] Furthermore, the crops include, but are not limited to, wheat, corn, rice, cotton, beans, rapeseed, etc. The licorice-goji berry-based carbon quantum dots of the present invention can improve the fresh weight and SPAD value of the above crops.
[0033] The present invention will be described below through specific embodiments. Those skilled in the art will understand that the specific embodiments below are merely illustrative and do not limit the scope of the invention in any way. Furthermore, in the following embodiments, unless otherwise specified, the reagents and equipment used are commercially available. If specific processing conditions and methods are not explicitly described in the later embodiments, conditions and methods known in the art can be used for processing. Example 1
[0034] This embodiment provides a method for preparing licorice-goji-based carbon quantum dots, the steps of which are as follows:
[0035] Goji berries and licorice were crushed into powders. 200 mg of goji berry powder and 200 mg of licorice powder were weighed out, mixed well, and 20 ml of distilled water were added. The mixture was subjected to a hydrothermal reaction in a polytetrafluoroethylene reactor at a temperature of 160°C for 6 hours. After cooling, the mixture was dispensed into 1.5 ml centrifuge tubes and centrifuged at 5000 rpm for 1 hour. The supernatant was collected to obtain a brown liquid with a sweet aroma, which is licorice-goji berry-based carbon quantum dots, denoted as LWCMs. The LWCMs were stored in a refrigerator at 4°C for later use.
[0036] LWCMs were dropped onto a copper grid, and their morphology was observed using a transmission electron microscope with an accelerating voltage of 200 kV. Figure 1 As shown, LWCMs are structures consisting of carbon spheres of about 20 nm loaded with carbon quantum dots. Example 2
[0037] Example 2 is basically the same as Example 1, except that 267 mg of wolfberry powder and 133 mg of licorice powder are weighed. Example 3
[0038] Example 3 is basically the same as Example 1, except that 160 mg of wolfberry powder and 240 mg of licorice powder are weighed. Example 4
[0039] Example 4 is basically the same as Example 1, except that 133 mg of wolfberry powder and 267 mg of licorice powder are weighed. Example 5
[0040] Example 5 is basically the same as Example 1, except that 13.3 ml of distilled water is added after mixing. Example 6
[0041] Example 6 is basically the same as Example 1, except that 40 ml of distilled water is added after mixing.
[0042] Comparative Example 1
[0043] This comparative example provides a method for preparing carbon quantum dots from wolfberry, the steps of which are as follows:
[0044] Grind goji berries into powder, weigh 200 mg of goji berry powder, add 10 ml of purified water, and carry out a hydrothermal reaction in a polytetrafluoroethylene reactor at a reaction temperature of 160℃ for 6 hours. After cooling, remove and dispense into 1.5 ml centrifuge tubes, centrifuge at 5000 rpm for 1 hour, and take the supernatant to obtain a brown liquid with a sweet aroma, which is goji berry carbon quantum dots, denoted as WoCDs. Store WoCDs in a refrigerator at 4℃ for later use.
[0045] WoCDs were dropped onto a copper grid, and their morphology was observed using a transmission electron microscope with an accelerating voltage of 200 kV. Figure 1 As shown, WoCDs are the structure of carbon quantum dots.
[0046] Comparative Example 2
[0047] This comparative example provides a method for preparing licorice carbon quantum dots, the steps of which are as follows:
[0048] Licorice was crushed into powder. 200 mg of licorice powder was weighed and added to 10 ml of purified water. The mixture was subjected to a hydrothermal reaction in a polytetrafluoroethylene reactor at a temperature of 160°C for 6 hours. After cooling, the mixture was dispensed into 1.5 ml centrifuge tubes and centrifuged at 5000 rpm for 1 hour. The supernatant was collected to obtain a brown liquid with a sweet aroma, which is licorice carbon quantum dots, denoted as LiCBs. The LiCBs were stored in a refrigerator at 4°C for later use.
[0049] LiCBs were dropped onto a copper grid, and their morphology was observed using a transmission electron microscope with an accelerating voltage of 200 kV. Figure 1 As shown, LiCBs are mesoporous carbon spheres with a diameter of approximately 200 nm.
[0050] Comparative Example 3
[0051] Comparative Example 3 is basically the same as Example 1, except that 300 mg of wolfberry powder and 100 mg of licorice powder are weighed.
[0052] Comparative Example 4
[0053] Comparative Example 4 is basically the same as Example 1, except that 100 mg of wolfberry powder and 300 mg of licorice powder are weighed.
[0054] The following experiments investigate the effects of carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 on crop salt tolerance when used as drip irrigation fertilizer.
[0055] Select plump, uniformly sized, and undamaged wheat seeds. Disinfect their surface with a 0.5-1% sodium hypochlorite solution for 10-15 minutes, then rinse with deionized water for 4-6 minutes. Allow the disinfected seeds to air dry at room temperature. Sow 5 seeds into each seed germination bag and add 15ml of distilled water.
[0056] The carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 were dissolved and diluted to 50-300 mg / L and then used to soak crop roots for 1-5 hours. After soaking, the crop seedlings were placed in the dark to adapt for 2-5 hours and then subjected to salt stress with 50-300 mM NaCl solution. The fresh weight and SPAD value of the crops were measured.
[0057] Experimental Example 1
[0058] This experiment was used to study the effect of the concentration of carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 after dissolution and dilution on the salt tolerance of crops.
[0059] The carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 were dissolved and diluted to 50 mg / L, 100 mg / L, 150 mg / L, 200 mg / L and 300 mg / L, respectively, and then used to soak wheat roots. Water was used as a control. The soaking time was 3 h. After soaking, the wheat seedlings were placed in the dark to adapt for 3 h and then subjected to salt stress with 300 mM NaCl solution. The fresh weight and SPAD value of the crops were measured. The results are shown in Tables 1-3.
[0060]
[0061]
[0062]
[0063] As shown in Tables 1-3, when the carbon quantum dot concentration was 50-300 mg / L, the aboveground and underground fresh weights of wheat seedlings treated with licorice-goji-based carbon quantum dots were significantly greater than those of wheat seedlings treated with licorice carbon quantum dots or goji berry carbon quantum dots alone. Furthermore, the SPAD value of wheat seedlings treated with licorice-goji-based carbon quantum dots was significantly greater than that of wheat seedlings treated with licorice carbon quantum dots or goji berry carbon quantum dots alone.
[0064] Experimental Example 2
[0065] This experiment was conducted to investigate the effects of using carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 as drip irrigation fertilizer on crop salt tolerance under different concentrations of salt stress.
[0066] The carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 were dissolved and diluted to 150 mg / L and then used to soak wheat roots. Water was used as a control. The soaking time was 3 h. After soaking, the wheat seedlings were placed in the dark to adapt for 3 h. Then, they were subjected to salt stress by 0, 50 mM, 100 mM, 150 mM, 200 mM and 300 mM NaCl solutions, respectively. The fresh weight and SPAD value of the crops were measured. The results are shown in Tables 4-6.
[0067]
[0068]
[0069]
[0070] As shown in Table 4-6, after different concentrations of salt stress, the aboveground and underground fresh weights of wheat seedlings treated with licorice-goji-based carbon quantum dots were significantly greater than those treated with licorice carbon quantum dots or goji berry carbon quantum dots alone. In addition, after different concentrations of salt stress, the SPAD values of wheat seedlings treated with licorice-goji-based carbon quantum dots were significantly greater than those treated with licorice carbon quantum dots or goji berry carbon quantum dots alone.
[0071] Experimental Example 3
[0072] This experiment was conducted to study the effects of carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 on the salt tolerance of different types of crops when used as drip irrigation fertilizer.
[0073] The carbon quantum dots prepared in Examples 1-6 and Comparative Examples 1-4 were dissolved and diluted to 150 mg / L and then used to soak crop roots for 3 hours. After soaking, wheat, corn, rice, cotton, soybean, pea, tomato, grape, sugar beet, rapeseed and alfalfa seedlings were placed in the dark for 3 hours to adapt, and then subjected to salt stress with 300 mM NaCl solution. After 5 days of stress, the fresh weight and SPAD value of the crops were measured. The results are shown in Tables 7-9.
[0074]
[0075]
[0076]
[0077] As shown in Tables 7-9, drip irrigation and fertilization of various crops using the licorice-goji-based carbon quantum dots of the present invention can effectively increase the fresh weight of various crops under salt stress; and drip irrigation and fertilization of various crops using the licorice-goji-based carbon quantum dots of the present invention can effectively increase the SPAD value of various crops under salt stress.
[0078] In the description of this specification, the terms "one embodiment," "another embodiment," "yet another embodiment," "some embodiments," "some specific embodiments," "other specific embodiments," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment, which are included in at least one embodiment of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples, without contradiction. Additionally, it should be noted that in this specification, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features.
[0079] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.
Claims
1. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer, characterized in that, The application of licorice-goji berry-based carbon quantum dots as drip irrigation fertilizer to improve crop salt tolerance; The preparation method of the licorice-goji-based carbon quantum dots is as follows: Goji berry powder and licorice powder are mixed and then added to a solvent, heated to react, cooled and separated, and the supernatant is taken and stored at 3-5℃ for later use. The mass ratio of the wolfberry powder to the licorice powder is 1:(0.5-2); The ratio of the sum of the masses of the wolfberry powder and licorice powder to the volume of the solvent is (10-30) mg: 1 ml; The solvent is distilled water; The heating reaction is carried out at a temperature of 150-180℃ for 4-8 hours.
2. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer according to claim 1, characterized in that, The separation method is centrifugal separation, and the centrifugation is performed at 2000-10000 rpm for 30-90 minutes.
3. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer according to claim 1 or 2, characterized in that, The licorice-goji-based carbon quantum dots are dissolved and diluted to obtain a nanomaterial solution. The nanomaterial solution is then used to soak the roots of crop seedlings.
4. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer according to claim 3, characterized in that, The concentration of the nanomaterial solution is 50-300 mg / L; Soaking time is 1-5 hours.
5. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer according to claim 3, characterized in that, After soaking, the crop seedlings were placed in the dark for 2-5 hours to adapt, and then subjected to salt stress with 50-500mM NaCl solution. The changes in the crop seedlings were recorded.
6. The application of licorice-lycium-based carbon quantum dots in crop drip irrigation fertilizer according to any one of claims 1, 2, 4, or 5, characterized in that, The crops include wheat, corn, rice, cotton, beans, and rapeseed.