Triple Fluorescent PCR Detection Method for Differentially Diagnosing Three Genes of Different African Swine Fever Viral Strains
By designing specific primer probes and constructing triple fluorescent PCR system, the problem of simultaneous detection of three genes of African swine fever virus is solved, and high-sensitivity virus detection and early diagnosis is achieved, which is suitable for a variety of sample types.
CN118755879BActive Publication Date: 2025-08-05CHINA INST OF VETERINARY DRUG CONTROL
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Patent Information
- Application Number
- CN202410737602.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-07
- Publication Date
- 2025-08-05
- Estimated Expiration
- 2044-06-07
AI Technical Summary
Technical Problem
The prior art cannot detect the three genes of African swine fever virus B646L, CD2v and I177L, especially the CD2v gene deletion strain, which leads to the increased difficulty in preventing and controlling African swine fever.
Method used
Design specific primer probes to target B646L, CD2v and I177L genes, build a triple fluorescent PCR system, optimize the ratio and reaction procedures of primer probes, and achieve simultaneous detection of these three genes.
Benefits of technology
It has achieved high sensitivity detection of African swine fever virus, can identify CD2v and I177L gene deletion strains, and has no cross-reactivity with other pig diseases. It is suitable for detection of multiple sample types and provides early diagnosis and monitoring methods.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application relates to the field of veterinary diagnostic technology, and specifically to a probe composition, a primer composition, and a triple fluorescence PCR kit and related methods for differential diagnosis of three genes of different strains of African swine fever composed of the above-mentioned probe composition and primer composition. The kit includes primer probes specific to the B646L gene, the CD2v gene, and the I177L gene. The present application designs fluorescent PCR primers and probes for the B646L gene, the CD2v gene, and the I177L gene, respectively, and establishes a triple fluorescence PCR detection method, which can quickly identify African swine fever virus strains and gene-deficient strains. At the same time, it has no cross-reaction with swine fever virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, porcine epidemic diarrhea virus, porcine circovirus, porcine parvovirus, healthy whole blood, oropharyngeal swabs, nasal swabs, anal swabs, environmental swabs, pig tissues, etc., and has good specificity.
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