A composition containing Antrodia cinnamomea powder and its preparation method and application

Abstract

The present invention relates to a composition containing Antrodia cinnamomea powder and a preparation method and application thereof, belonging to the technical field of medicines, the composition comprises Antrodia cinnamomea extract, Hovenia dulcis extract, Pueraria root extract, Psoralea corylifolia extract, Cyperus rotundus extract and cotton sugar; Antrodia cinnamomea extract contains triterpenoid compounds; Pueraria root extract is an extract below 10kDa obtained by graded enzymolysis of Pueraria root with hemicellulase and trypsin; Psoralea corylifolia extract is an extract below 5kDa obtained by enzymolysis of Psoralea corylifolia protein with alkaline protease; Cyperus rotundus extract is an extract below 5kDa obtained by enzymolysis of Cyperus rotundus with subtilisin and trypsin. Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract all have the effect of promoting hangover, and have a significant synergistic effect when used in combination with Antrodia cinnamomea extract, can promote cell and enzyme activity, promote the decomposition of alcohol in the body, quickly improve the drunken state, and do not cause vomiting.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines, and particularly relates to a composition containing Antrodia cinnamomea powder, and a preparation method and application thereof. Background Art

[0002] Antrodia cinnamomea is a precious medicinal material that has the effects of eliminating carbuncle and dispersing nodules, clearing away heat and detoxifying, clearing liver and purging fire, dispersing blood stasis and relieving pain, and relieving sore throat; it has a good therapeutic effect on liver diseases, stagnation of qi and blood, abdominal distension and pain, amenorrhea, dysmenorrhea, carbuncle, sore, weak vital energy, physical overdraft and other symptoms. Antrodia cinnamomea also has the effects of lowering blood pressure, blood lipids, blood sugar and cholesterol; it can also inhibit platelet aggregation and enhance the body's immunity; and it can effectively prevent arteriosclerosis, cerebral thrombosis, cerebral stroke and myocardial infarction and other diseases.

[0003] Antrodia cinnamomea contains polysaccharides, triterpenoids, superoxide dismutase, adenosine, proteins (including immune proteins), vitamins, trace elements, nucleic acids, lectins, amino acids, linoleic acid, cellulose, blood pressure stabilizing substances, etc. In particular, the content of triterpenoids in Antrodia cinnamomea is 15 times that of top-grade Ganoderma lucidum and more than 30 times that of ordinary Ganoderma lucidum. Triterpenoids have a wide range of physiological activities, including hemolysis, anti-cancer, anti-inflammatory, antibacterial, antiviral, analgesic, cholesterol-lowering, and molluscicide.

[0004] In addition, Antrodia cinnamomea also has a certain effect of sobering up, but the existing sobering up drugs containing Antrodia cinnamomea have the problem of unsatisfactory sobering up effects. On the one hand, the drunken person's stomach is sensitive and prone to vomiting. Antrodia cinnamomea tastes bitter, pungent and cold in nature. When taken in a drunken state, compliance is poor, which can easily induce vomiting and lose its efficacy. On the other hand, the sobering up drugs themselves have the problem of slow sobering up effects and unsatisfactory efficacy, so they are not widely used. Summary of the invention

[0005] The existing Antrodia cinnamomea powder as a hangover-relieving ingredient has poor compliance and is easy to induce vomiting, and the hangover-relieving drugs themselves have slow hangover-relieving effects and unsatisfactory efficacy. The present invention provides a composition containing Antrodia cinnamomea powder and a preparation method and application thereof, the composition containing Antrodia cinnamomea extract, Hovenia dulcis extract, Pueraria root extract, Trillium extract, Cyperus rotundus extract and cotton sugar; wherein Pueraria root extract, Trillium extract and Cyperus rotundus extract all have the effect of promoting hangover-relieving, and the combination of Pueraria root extract, Trillium extract and Cyperus rotundus extract has a significant synergistic effect on Antrodia cinnamomea extract, which can quickly improve the drunken state without vomiting. The specific technical scheme is as follows:

[0006] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by weight: 6 to 10 parts of Antrodia cinnamomea extract, 1 to 3 parts of Hovenia dulcis extract, 0.5 to 2 parts of Pueraria root extract, 0.5 to 1.5 parts of Psoralea corylifolia extract, 3 to 5 parts of Cyperus rotundus extract and 0.1 to 0.3 parts of cotton sugar;

[0007] The Antrodia cinnamomea extract contains triterpenoid compounds; the Pueraria root extract is an extract of less than 10 kDa obtained by graded enzymatic hydrolysis of Pueraria root with hemicellulase and trypsin; the Trillium trifoliatum extract is an extract of less than 5 kDa obtained by enzymatic hydrolysis of Trillium trifoliatum protein with alkaline protease; and the Cyperus rotundus extract is an extract of less than 5 kDa obtained by enzymatic hydrolysis of Cyperus rotundus with subtilisin and trypsin.

[0008] In the above technical scheme, the preparation method of the Antrodia cinnamomea extract is: wash the Antrodia cinnamomea with water, dry it at a low temperature of 25°C to 30°C or dry it naturally, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in alcohol; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate, and take the supernatant; evaporate the supernatant to remove the alcohol, air-dry it, and grind it into powder to obtain the Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0009] The alcohol is edible alcohol, and the volume concentration of the alcohol is 92% to 95%; the soaking time is 3d to 7d; the liquid level of the alcohol is higher than the height of the bag; the precipitation time is 1h to 3h; the evaporation temperature is 60°C to 65°C; and the air-drying temperature is 30°C to 40°C.

[0010] In the above technical scheme, the preparation method of the Hovenia dulcis extract is: crushing the Hovenia dulcis, using ethanol 6 to 8 times the mass of Hovenia dulcis to perform percolation to obtain a percolation liquid, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0011] In the above technical scheme, the preparation method of the kudzu root extract is: crush the kudzu root, soak it in water 6 to 8 times the mass of the kudzu root for 1h to 2h, filter, take the filter residue, dry it, and then disperse the filter residue in water 6 to 8 times the mass of the filter residue, adjust the pH to 4.0 to 5.5, add hemicellulase, hydrolyze at 50°C to 55°C for 1.5h to 2h, reduce to 36°C to 38°C, adjust the pH to 7.8 to 8.5, add trypsin, hydrolyze for 1.5h to 2h, inactivate the enzyme at high temperature, reduce to room temperature, filter with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry, and obtain the kudzu root extract.

[0012] The amount of hemicellulase added is 0.5% to 1.5% of the mass of kudzu root; the amount of trypsin added is 0.5% to 1.5% of the mass of kudzu root; the temperature of high temperature enzyme inactivation is 90°C to 100°C, and the time is 20min to 30min.

[0013] In the above technical scheme, the preparation method of the Pseudo-Trillium extract is as follows: taking Pseudo-Trillium to break the spores and crushing them, adding 0.12 mol / L to 0.18 mol / L salt water, soaking for 1h to 2h, centrifuging, taking the supernatant, adding 4 mol / L to 4.5 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water 6 to 8 times the mass of the protein precipitate, adjusting the pH to 10.0 to 11.0, adding 0.5% to 1.5% alkaline protease by mass of the protein precipitate, enzymolysis at 40°C to 50°C for 1.5h to 2h, high temperature enzyme inactivation at 90°C to 100°C for 20min to 30min, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the Pseudo-Trillium extract.

[0014] In the above technical scheme, the preparation method of the Cyperus rotundus extract is: crushing the Cyperus rotundus, adding water 6 to 8 times the mass of the Cyperus rotundus, adjusting the pH to 7.8 to 8.0, adding 0.5% to 1.5% by mass of subtilisin and 0.5% to 1.5% by mass of trypsin, hydrolyzing at 35°C to 38°C for 1h to 1.5h, inactivating the enzyme at 90°C to 100°C for 20min to 30min, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0015] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0016] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0017] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0018] S1: According to the mass ratio, the composition: polyethylene glycol = (6-10): (1-1.5), the composition and polyethylene glycol powder are evenly mixed, placed in drip irrigation, stirred and blended in an oil bath at 70°C-75°C for 1h-2h, and dripped into edible oil to make drip pills; the dripping parameters are: the nozzle temperature of the dripping is 30°C-35°C, the dripper is 2cm-3cm away from the molding, and the cooling temperature of the edible oil is 10°C-12°C;

[0019] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 7d to 15d to obtain dripping pills.

[0020] The present invention provides a composition containing Antrodia cinnamomea powder, a preparation method thereof and an application thereof, which have the following beneficial effects compared with the prior art:

[0021] 1. The composition of the present invention contains Antrodia cinnamomea extract, Hovenia dulcis extract, Pueraria root extract, Psoralea corylifolia extract, Cyperus rotundus extract and cotton sugar; wherein, Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract all have the effect of promoting hangover relief, and the combination of Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract has a significant synergistic effect on Antrodia cinnamomea extract, which can quickly improve the state of drunkenness without causing vomiting.

[0022] 2. The combination of Hovenia dulcis extract and Antrodia cinnamomea extract has a good hangover-relieving effect.

[0023] 3. Soft sugar can improve the taste of the composition, improve palatability, and increase medication compliance.

[0024] 4. The Antrodia cinnamomea extract of the present invention contains a higher proportion of triterpenoid compounds, and has a better hangover-relieving effect than Antrodia cinnamomea dry powder, and can lower blood pressure and blood lipids and reduce poisoning reactions.

[0025] 5. The kudzu root extract of the present invention is an extract below 10 kDa obtained by graded enzymatic hydrolysis of kudzu root with hemicellulase and trypsin. Compared with ordinary ethanol percolation extract, the kudzu root extract of the present invention also contains small molecule peptides and free amino acids, as well as other decomposition products, which can be quickly absorbed, assisting the Antrodia cinnamomea extract in improving the damage of ethanol, and repairing and improving liver function.

[0026] 6. The Psoralea corylifolia extract of the present invention is an extract below 5 kDa obtained by enzymatic hydrolysis of Psoralea corylifolia protein by alkaline protease. Psoralea corylifolia protein peptides and amino acids can be quickly absorbed and improve liver function. The inventor speculates that the Psoralea corylifolia extract can promote the improvement of alcohol dehydrogenase and acetaldehyde dehydrogenase activities; it is unexpected that the Psoralea corylifolia extract has a good effect of promoting alcohol sobering up.

[0027] 7. The Cyperus rotundus extract of the present invention is an extract of less than 5 kDa obtained by enzymatic hydrolysis of Cyperus rotundus with Bacillus subtilisin and trypsin, contains various active ingredients, and can repair and improve liver function. The inventors speculate that the Cyperus rotundus extract can promote and increase the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, and can shorten the speed of hangover resolution; it is unexpected that the Cyperus rotundus extract has a good effect in promoting hangover resolution.

[0028] 8. The present invention's Antrodia camphorata extract, Hovenia dulcis extract, Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract are scientifically and rationally compounded to promote cell and enzyme activity, promote the decomposition of alcohol in the body, have very excellent effects of sobering up and sobering up, protect the liver, and repair liver health. In addition to sobering up, Antrodia camphorata powder also has the benefits of clearing away heat and detoxifying, eliminating carbuncle and dispersing furuncle, clearing the liver and purging fire, and promoting the recovery of the drunk body to a healthy state. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0030] Example 1

[0031] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by mass: 8 parts of Antrodia cinnamomea extract, 2 parts of Hovenia dulcis extract, 1 part of Pueraria root extract, 1 part of Psoralea corylifolia extract, 4 parts of Cyperus rotundus extract and 0.2 parts of cotton sugar.

[0032] Among them, the preparation method of Antrodia cinnamomea extract is as follows: wash Antrodia cinnamomea with water, dry it at a low temperature of 30°C, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in edible alcohol with a volume concentration of 95% for 4 days. During the soaking, the liquid level of the edible alcohol is higher than the height of the cloth bag; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate for 2 hours, and take the supernatant; evaporate the supernatant at 60°C to remove the edible alcohol, air-dry it at 35°C, and grind it into powder to obtain Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0033] The preparation method of the Hovenia dulcis extract is as follows: crushing the Hovenia dulcis, performing percolation with ethanol 7 times the mass of the Hovenia dulcis to obtain a percolation solution, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0034] The preparation method of kudzu root extract is as follows: pulverize kudzu root, soak it in water with 7 times the mass of kudzu root for 1.5 hours, filter, take the filter residue, dry it, then disperse the filter residue in water with 7 times the mass of the filter residue, adjust the pH to 5.0, add 1% hemicellulase with the mass of kudzu root, enzymolyze it at 52°C for 1.5 hours, reduce the pH to 37°C, adjust the pH to 8.0, add 1% trypsin with the mass of kudzu root, enzymolyze it for 1.5 hours, inactivate the enzyme at 95°C for 25 minutes, reduce the pH to room temperature, filter it with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry it, and obtain kudzu root extract.

[0035] The preparation method of the trillium extract is as follows: taking trillium to break the spores, adding 0.15 mol / L salt water, soaking for 1.5 hours, centrifuging at 12000 r / min for 20 minutes, taking the supernatant, adding 4.2 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging at 12000 r / min for 20 minutes to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 7 times the mass of the protein precipitate, adjusting the pH to 10.5, adding alkaline protease with a mass of 1% of the mass of the protein precipitate, enzymolysis at 45°C for 2 hours, high-temperature enzyme inactivation at 95°C for 25 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the trillium extract.

[0036] The preparation method of the Cyperus rotundus extract is as follows: crushing the Cyperus rotundus, adding water 7 times the mass of the Cyperus rotundus, adjusting the pH to 8.0, adding 1% by mass of subtilisin and 1% by mass of trypsin, hydrolyzing at 37°C for 1.5h, inactivating the enzyme at 95°C for 25min, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0037] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0038] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0039] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0040] S1: According to the mass ratio, the composition: polyethylene glycol = 8:1.2, the composition and polyethylene glycol powder are evenly mixed, placed in drip irrigation, stirred and fused in an oil bath at 70°C for 1h, and dripped into edible oil to make drip pills; the dripping parameters are: the dripping nozzle temperature is 30°C, the dripper is 2cm away from the molding, and the cooling temperature of the edible oil is 10°C;

[0041] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 10 days to fuse, and obtain dripping pills.

[0042] Example 2

[0043] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by mass: 10 parts of Antrodia cinnamomea extract, 3 parts of Hovenia dulcis extract, 2 parts of Pueraria root extract, 1.5 parts of Trillium extract, 5 parts of Cyperus rotundus extract and 0.3 parts of cotton sugar.

[0044] Among them, the preparation method of Antrodia cinnamomea extract is as follows: wash Antrodia cinnamomea with water, dry it naturally at 25°C, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in edible alcohol with a volume concentration of 95% for 7 days. During the soaking, the liquid level of the edible alcohol is higher than the height of the cloth bag; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate for 3 hours, and take the supernatant; evaporate the supernatant at 65°C to remove the edible alcohol, air-dry it at 40°C, grind it into powder, and obtain Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0045] The preparation method of the Hovenia dulcis extract is as follows: crushing the Hovenia dulcis, performing percolation with ethanol in an amount of 8 times the mass of the Hovenia dulcis to obtain a percolation liquid, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0046] The preparation method of the kudzu root extract is as follows: crush the kudzu root, soak it in water with an amount of 8 times the mass of the kudzu root for 2 hours, filter, take the filter residue, dry it, then disperse the filter residue in water with an amount of 8 times the mass of the filter residue, adjust the pH to 5.5, add hemicellulase with an amount of 1.5% by mass of the kudzu root, perform enzymolysis at 55°C for 2 hours, reduce the pH to 38°C, adjust the pH to 8.5, add trypsin with an amount of 1.5% by mass of the kudzu root, perform enzymolysis for 2 hours, inactivate the enzyme at 100°C for 30 minutes, reduce the pH to room temperature, filter with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry, and obtain the kudzu root extract.

[0047] The preparation method of the trillium extract is as follows: taking trillium to break the spores, adding 0.18 mol / L salt water, soaking for 2 hours, centrifuging at 15000 r / min for 25 minutes, taking the supernatant, adding 4.5 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging at 15000 r / min for 25 minutes to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 8 times the mass of the protein precipitate, adjusting the pH to 11.0, adding alkaline protease with a mass of 1.5% of the mass of the protein precipitate, enzymolysis at 50°C for 2 hours, high temperature enzyme inactivation at 100°C for 30 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the trillium extract.

[0048] The preparation method of the Cyperus rotundus extract is as follows: crushing the Cyperus rotundus, adding water 8 times the mass of the Cyperus rotundus, adjusting the pH to 8.0, adding 1.5% by mass of subtilisin and 1.5% by mass of trypsin, hydrolyzing at 38°C for 1.5 hours, inactivating the enzyme at 100°C for 30 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0049] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0050] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0051] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0052] S1: According to the mass ratio, the composition: polyethylene glycol = 6:1, the composition and polyethylene glycol powder are evenly mixed, placed in drip irrigation, stirred and fused in an oil bath at 75°C for 2h, and dripped into edible oil to make drip pills; the dripping parameters are: the dripping nozzle temperature is 35°C, the dripper is 3cm away from the molding, and the cooling temperature of the edible oil is 12°C;

[0053] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 15 days to fuse, and obtain dripping pills.

[0054] Example 3

[0055] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by mass: 6 parts of Antrodia cinnamomea extract, 1 part of Hovenia dulcis extract, 0.5 parts of Pueraria root extract, 0.5 parts of Psoralea corylifolia extract, 3 parts of Cyperus rotundus extract and 0.1 parts of cotton sugar.

[0056] Among them, the preparation method of Antrodia cinnamomea extract is: wash Antrodia cinnamomea with water, dry it at a low temperature of 28°C, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in edible alcohol with a volume concentration of 92% for 3 days. During the soaking, the liquid level of the edible alcohol is higher than the height of the cloth bag; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate for 1 hour, and take the supernatant; evaporate the supernatant at 60°C to remove the edible alcohol, air-dry it at 30°C, grind it into powder, and obtain Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0057] The preparation method of the Hovenia dulcis extract is as follows: crushing the Hovenia dulcis, performing percolation with ethanol 6 times the mass of the Hovenia dulcis to obtain a percolation solution, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0058] The preparation method of the kudzu root extract is as follows: crush the kudzu root, soak it in water 6 times the mass of the kudzu root for 1 hour, filter, take the filter residue, dry it, then disperse the filter residue in water 6 times the mass of the filter residue, adjust the pH to 4.0, add 0.5% hemicellulase by mass of the kudzu root, enzymolyze it at 50°C for 1.5 hours, reduce the temperature to 36°C, adjust the pH to 7.8, add 0.5% trypsin by mass of the kudzu root, enzymolyze it for 1.5 hours, inactivate the enzyme at 90°C for 20 minutes, reduce the temperature to room temperature, filter it with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry it, and obtain the kudzu root extract.

[0059] The preparation method of the trillium extract is as follows: taking trillium to break the spores, adding 0.12 mol / L salt water, soaking for 1 hour, centrifuging at 10000 r / min for 15 minutes, taking the supernatant, adding 4 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging at 10000 r / min for 15 minutes to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 6 times the mass of the protein precipitate, adjusting the pH to 10.0, adding alkaline protease with a mass of 0.5% of the mass of the protein precipitate, enzymolysis at 40°C for 1.5 hours, high temperature enzyme inactivation at 90°C for 20 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the trillium extract.

[0060] The preparation method of the Cyperus rotundus extract is as follows: crushing the Cyperus rotundus, adding water 6 times the mass of the Cyperus rotundus, adjusting the pH to 7.8, adding 0.5% by mass of subtilisin and 0.5% by mass of trypsin, hydrolyzing at 35°C for 1 hour, inactivating the enzyme at 90°C for 20 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0061] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0062] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0063] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0064] S1: According to the mass ratio, the composition: polyethylene glycol = 10:1.5, the composition and polyethylene glycol powder are mixed evenly, placed in drip irrigation, stirred and fused in an oil bath at 70°C for 1h, and dripped into edible oil to make drip pills; the dripping parameters are: the dripping nozzle temperature is 30°C, the dripper is 2cm away from the molding, and the cooling temperature of the edible oil is 10°C;

[0065] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 7 days to fuse, and obtain dripping pills.

[0066] Example 4

[0067] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by mass: 7 parts of Antrodia cinnamomea extract, 1.5 parts of Hovenia dulcis extract, 0.8 parts of Pueraria root extract, 0.8 parts of Psoralea corylifolia extract, 3.5 parts of Cyperus rotundus extract and 0.15 parts of cotton sugar.

[0068] Among them, the preparation method of Antrodia cinnamomea extract is: wash Antrodia cinnamomea with water, dry it at a low temperature of 29°C, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in edible alcohol with a volume concentration of 93% for 4 days. During the soaking, the liquid level of the edible alcohol is higher than the height of the cloth bag; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate for 1.5 hours, and take the supernatant; evaporate the supernatant at 62°C to remove the edible alcohol, air-dry it at 32°C, grind it into powder, and obtain Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0069] The preparation method of the Hovenia dulcis extract is as follows: crushing the Hovenia dulcis, performing percolation with ethanol 6.5 times the mass of the Hovenia dulcis to obtain a percolation solution, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0070] The preparation method of the kudzu root extract is as follows: crush the kudzu root, soak it in water with a mass of 6.5 times the mass of the kudzu root for 1 hour, filter, take the filter residue, dry it, then disperse the filter residue in water with a mass of 6.5 times the mass of the filter residue, adjust the pH to 4.2, add 0.8% hemicellulase by mass of the kudzu root, enzymolyze it at 52°C for 1.5 hours, reduce the pH to 37°C, adjust the pH to 8.0, add 0.8% trypsin by mass of the kudzu root, enzymolyze it for 1.5 hours, inactivate the enzyme at 92°C for 22 minutes, reduce the pH to room temperature, filter it with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry it, and obtain the kudzu root extract.

[0071] The preparation method of the trillium extract is as follows: taking trillium to break the spores, adding 0.13 mol / L salt water, soaking for 1 hour, centrifuging at 10000 r / min for 18 minutes, taking the supernatant, adding 8.2 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging at 10000 r / min for 20 minutes to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 6.5 times the mass of the protein precipitate, adjusting the pH to 10.2, adding alkaline protease with a mass of 0.8% of the protein precipitate, enzymolysis at 42°C for 1.5 hours, high temperature enzyme inactivation at 92°C for 22 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the trillium extract.

[0072] The preparation method of the Cyperus rotundus extract is as follows: crushing the Cyperus rotundus, adding 6.5 times the mass of water of the Cyperus rotundus, adjusting the pH to 7.9, adding 0.8% by mass of subtilisin and 0.8% by mass of trypsin of the Cyperus rotundus, performing enzymatic hydrolysis at 36°C for 1 hour, inactivating the enzyme at 92°C for 22 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0073] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0074] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0075] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0076] S1: According to the mass ratio, the composition: polyethylene glycol = 7:1, the composition and polyethylene glycol powder are mixed evenly, placed in drip irrigation, stirred and fused in an oil bath at 72°C for 1h, and dripped into edible oil to make drip pills; the dripping parameters are: the dripping nozzle temperature is 32°C, the dripper is 2.5cm away from the molding, and the cooling temperature of the edible oil is 11°C;

[0077] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 8 days to fuse, and obtain dripping pills.

[0078] Example 5

[0079] A composition containing Antrodia cinnamomea powder, the composition is prepared from the following raw materials in parts by mass: 9 parts of Antrodia cinnamomea extract, 2.5 parts of Hovenia dulcis extract, 1.5 parts of Pueraria root extract, 1.3 parts of Psoralea corylifolia extract, 4.5 parts of Cyperus rotundus extract and 0.25 parts of cotton sugar.

[0080] Among them, the preparation method of Antrodia cinnamomea extract is as follows: wash Antrodia cinnamomea with water, dry it naturally at 26°C, grind it into fine powder, put it in a cloth bag, and soak the cloth bag in edible alcohol with a volume concentration of 94% for 6 days. During the soaking, the liquid level of the edible alcohol is higher than the height of the cloth bag; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate for 2.5 hours, and take the supernatant; evaporate the supernatant at 64°C to remove the edible alcohol, air-dry it at 38°C, and grind it into powder to obtain Antrodia cinnamomea extract, that is, Antrodia cinnamomea powder, which contains triterpenoid compounds.

[0081] The preparation method of the Hovenia dulcis extract is as follows: crushing the Hovenia dulcis, performing percolation with ethanol 7.5 times the mass of the Hovenia dulcis to obtain a percolation solution, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis extract.

[0082] The preparation method of the kudzu root extract is as follows: crush the kudzu root, soak it in water with a mass of 7.5 times the mass of the kudzu root for 2 hours, filter, take the filter residue, dry it, then disperse the filter residue in water with a mass of 7.5 times the mass of the filter residue, adjust the pH to 5.2, add 1.2% hemicellulase by mass of the kudzu root, enzymolyze it at 54°C for 2 hours, reduce the pH to 37.5°C, adjust the pH to 8.2, add 1.2% trypsin by mass of the kudzu root, enzymolyze it for 2 hours, inactivate the enzyme at 98°C for 28 minutes, reduce the pH to room temperature, filter it with a 10kDa ultrafiltration membrane, obtain a liquid below 10kDa, spray dry it, and obtain the kudzu root extract.

[0083] The preparation method of the Psoralea corylifolia extract is as follows: taking Psoralea corylifolia to break the spores, adding 0.16 mol / L salt water, soaking for 2 hours, centrifuging at 14000 r / min for 22 minutes, taking the supernatant, adding 4.4 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated, centrifuging at 14000 r / min for 22 minutes to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 7.5 times the mass of the protein precipitate, adjusting the pH to 10.8, adding alkaline protease with a mass of 1.2% of the protein precipitate, enzymolysis at 48°C for 2 hours, high-temperature enzyme inactivation at 98°C for 28 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the Psoralea corylifolia extract.

[0084] The preparation method of the Cyperus rotundus extract is as follows: crushing the Cyperus rotundus, adding 7.5 times the mass of water of the Cyperus rotundus, adjusting the pH to 8.0, adding 1.2% by mass of subtilisin and 1.2% by mass of trypsin of the Cyperus rotundus, performing enzymatic hydrolysis at 37.5°C for 11.5 hours, inactivating the enzyme at 98°C for 28 minutes, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, collecting liquid less than 5kDa, spray drying, and obtaining the Cyperus rotundus extract.

[0085] The preparation method of the above-mentioned composition containing Antrodia cinnamomea powder comprises the following steps:

[0086] The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

[0087] The above-mentioned composition containing Antrodia cinnamomea powder is used to prepare a drop pill for hangover relief, and the preparation method of the drop pill comprises the following steps:

[0088] S1: According to the mass ratio, the composition: polyethylene glycol = 9:14, the composition and polyethylene glycol powder are evenly mixed, placed in drip irrigation, stirred and fused in an oil bath at 74°C for 2h, and dripped into edible oil to make drip pills; the dripping parameters are: the dripping nozzle temperature is 34°C, the dripper is 3cm away from the molding, and the cooling temperature of the edible oil is 12°C;

[0089] S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 12 days to fuse, and obtain dripping pills.

[0090] Preparation Comparative Example 1

[0091] In the composition, the preparation method of the kudzu root extract is a conventional method: the kudzu root is crushed, ethanol of 7 times the mass of the kudzu root is used for percolation to obtain a percolation liquid, the ethanol is evaporated to obtain an extract, and the extract is dried and crushed to obtain the kudzu root extract. Other parameters and methods are the same as in Example 1.

[0092] Preparation Comparative Example 2

[0093] No Psoralea corylifolia extract was added to the composition; other parameters and methods were the same as those in Example 1.

[0094] Preparation Comparative Example 3

[0095] No Cyperus rotundus extract was added to the composition; other parameters and methods were the same as those in Example 1.

[0096] Preparation Comparative Example 4

[0097] In the composition, no Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract are added; other parameters and methods are the same as in Example 1.

[0098] The hangover-relief effects of the above-mentioned compositions of the embodiments and the compositions of the comparative examples were tested.

[0099] 100 rats (fasting, no food within 4 hours) were selected and divided into 10 groups, half of which were male and half were female; 9 groups were test groups, which were gavaged with 0.02g / Kg body weight of each composition; 1 group was not gavaged with the composition as a blank group. After gavage for 30 minutes, each rat was gavaged with 6g / Kg of edible alcohol with a volume concentration of 50%; the alcohol volume concentration was 80%.

[0100] Blood was collected from the mice at 0.5h, 1h, 1.5h, 2h, 4h, 6h, and 8h after intragastric administration of alcohol; the changes in blood alcohol concentration were determined by gas chromatography; the test results are shown in Table 1 below.

[0101] Table 1 Blood alcohol concentration change test results

[0102]

[0103] At the same time, the righting reflex test was performed, the sleeping time was the intoxication time, and the intoxication maintenance was counted. The results are shown in Table 2 below.

[0104] Table 2 Results of rat drunkenness maintenance time test (average value)

[0105]

[0106]

[0107] During the experiment, more than 50% of the rats in Comparative Example 4 and the blank sample vomited, while the rats in the other groups did not vomit.

[0108] From the above results, it can be seen that compared with the Pueraria lobata ethanol percolation extract, the Pueraria lobata enzymatic extract has a better auxiliary and alcohol-resolving effect on the Antrodia cinnamomea extract; the Psoralea corylifolia extract has a good auxiliary and alcohol-resolving effect on the Antrodia cinnamomea extract, and the Cyperus rotundus extract has an excellent auxiliary and alcohol-resolving effect on the Antrodia cinnamomea extract; the combination of Pueraria lobata extract, Psoralea corylifolia extract and Cyperus rotundus extract, when mixed with the Antrodia cinnamomea extract, has a significant synergistic effect, can promote the rapid decomposition of alcohol, and improve the state of drunkenness.

Claims

1. A composition containing Antrodia cinnamomea powder, characterized in that: The composition is prepared from the following raw materials in parts by weight: 6 to 10 parts of Antrodia cinnamomea extract, 1 to 3 parts of Hovenia dulcis extract, 0.5 to 2 parts of Pueraria root extract, 0.5 to 1.5 parts of Trillium extract, 3 to 5 parts of Cyperus rotundus extract and 0.1 to 0.3 parts of cotton sugar; The Antrodia cinnamomea extract contains triterpenoid compounds; the Pueraria root extract is an extract of less than 10 kDa obtained by graded enzymatic hydrolysis of Pueraria root with hemicellulase and trypsin; the Trillium trifoliatum extract is an extract of less than 5 kDa obtained by enzymatic hydrolysis of Trillium trifoliatum protein with alkaline protease; the Cyperus rotundus extract is an extract of less than 5 kDa obtained by enzymatic hydrolysis of Cyperus rotundus with subtilisin and trypsin; The preparation method of the Antrodia cinnamomea extract is as follows: wash Antrodia cinnamomea with water, dry at 25°C to 30°C or dry naturally, grind into fine powder, put into a cloth bag, and soak the cloth bag in alcohol; take out the cloth bag, centrifuge and dry it in a dehydrator, collect the centrifuge liquid and the soaking liquid, precipitate, and take the supernatant; evaporate the supernatant to remove the alcohol, air-dry, grind to powder, and obtain the Antrodia cinnamomea extract, i.e., Antrodia cinnamomea powder, which contains triterpenoid compounds; The preparation method of the Hovenia dulcis fruit extract comprises the following steps: crushing the Hovenia dulcis fruit, performing percolation with ethanol of 6 to 8 times the mass of the Hovenia dulcis fruit to obtain a percolation solution, evaporating and removing the ethanol to obtain an extract, drying, and crushing to obtain the Hovenia dulcis fruit extract.

2. A composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The alcohol is edible alcohol, and the volume concentration of the alcohol is 92% to 95%; the soaking time is 3 days to 7 days; the liquid level of the alcohol is higher than the height of the bag; the precipitation time is 1 hour to 3 hours; the evaporation temperature is 60°C to 65°C; and the air-drying temperature is 30°C to 40°C.

3. A composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The preparation method of the kudzu root extract comprises the following steps: crushing the kudzu root, soaking it in water of 6 to 8 times the mass of the kudzu root for 1 to 2 hours, filtering, taking the filter residue, drying, and then dispersing the filter residue in water of 6 to 8 times the mass of the filter residue, adjusting the pH to 4.0 to 5.5, adding hemicellulase, performing enzymolysis at 50 to 55° C. for 1.5 to 2 hours, reducing the pH to 36 to 38° C., adjusting the pH to 7.8 to 8.5, adding trypsin, performing enzymolysis for 1.5 to 2 hours, inactivating the enzyme at high temperature, reducing the pH to room temperature, filtering with a 10 kDa ultrafiltration membrane, obtaining a liquid below 10 kDa, and spray drying to obtain the kudzu root extract.

4. A composition containing Antrodia cinnamomea powder according to claim 3, characterized in that: The amount of hemicellulase added is 0.5% to 1.5% of the mass of Pueraria root; the amount of trypsin added is 0.5% to 1.5% of the mass of Pueraria root; the temperature of high-temperature enzyme inactivation is 90°C to 100°C, and the time is 20min to 30min.

5. The composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The preparation method of the trillium extract comprises the following steps: taking trillium to break spores, crushing, adding 0.12mol / L-0.18mol / L saline, soaking for 1h-2h, centrifuging, taking supernatant, adding 4mol / L-4.5mol / L ammonium sulfate solution to the supernatant until protein is completely precipitated, centrifuging to obtain protein precipitate; resuspending the protein precipitate in water with a mass of 6 to 8 times that of the protein precipitate, adjusting the pH to 10.0-11.0, adding alkaline protease with a mass of 0.5%-1.5% of the protein precipitate, performing enzymolysis at 40°C-50°C for 1.5h-2h, performing high temperature enzyme inactivation at 90°C-100°C for 20min-30min, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a liquid below 5kDa, spray drying, and obtaining the trillium extract.

6. The composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The preparation method of the Cyperus rotundus extract comprises the following steps: crushing the Cyperus rotundus, adding water 6 to 8 times the mass of the Cyperus rotundus, adjusting the pH to 7.8 to 8.0, adding 0.5% to 1.5% by mass of subtilisin and 0.5% to 1.5% by mass of trypsin, performing enzymatic hydrolysis at 35 to 38° C. for 1 hour to 1.5 hours, inactivating the enzyme at 90 to 100° C. for 20 minutes to 30 minutes, cooling to room temperature, filtering with a 5 kDa ultrafiltration membrane, collecting liquid less than 5 kDa, and spray drying to obtain the Cyperus rotundus extract.

7. The method for preparing a composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The preparation method of the composition comprises the following steps: The Antrodia cinnamomea extract, the Hovenia dulcis extract, the Pueraria root extract, the Psoralea corylifolia extract and the Cyperus rotundus extract are mixed evenly according to their weight proportions, and then soft sugar is added and mixed evenly to obtain a composition.

8. The use of a composition containing Antrodia cinnamomea powder according to claim 1, characterized in that: The composition is used to prepare a drop pill for sobering up, and the preparation method of the drop pill comprises the following steps: S1: According to the mass ratio, the composition: polyethylene glycol = (6-10): (1-1.5), the composition and polyethylene glycol are evenly mixed, placed in drip irrigation, stirred and blended in an oil bath at 70℃-75℃ for 1h-2h, and dripped into edible oil to make drip pills; the dripping parameters are: the nozzle temperature of the dripping is 30℃-35℃, the dripper is 2cm-3cm away from the molding, and the cooling temperature of the edible oil is 10℃-12℃; S2: After the dripping is completed, filter out the edible oil to obtain dripping pills, use food-grade kitchen oil-absorbing paper to repeatedly rub and wipe the oil on the dripping pills, let them stand for 7d to 15d to obtain dripping pills.

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