A fully automatic artificial root system multifunctional simulation test device

Abstract

The present invention discloses a fully automatic artificial root multifunctional simulation test device, which belongs to the technical field of plant root growth research, and comprises a base, wherein the side walls of the base are provided with a plurality of support platforms, wherein the plurality of support platforms are evenly arranged on the side walls of the base in a ring shape at equal intervals, a fixed column is installed at the center of the base, a fixed plate is installed on the upper wall of the fixed column, a telescopic component is movably installed in the fixed column, and the telescopic component is provided with a plurality of groups, which are telescopic component 1, telescopic component 2 ... telescopic component N from bottom to top, the telescopic component 1 is movably sleeved in the fixed column, the telescopic component 2 is movably sleeved in the telescopic component 1, and the telescopic component N is movably sleeved in the telescopic component N-1; a lifting component is also installed at the center of the base, and the lifting component is arranged through the telescopic component N. The device can carry out a plurality of tests and can also form a protection for the test box to reduce vibration during movement.

Description

Technical Field

[0001] The invention belongs to the technical field of plant root growth research, and in particular relates to a full-automatic artificial root multifunctional simulation test device. Background Art

[0002] The root system of plants is not only an organ of absorption and metabolism, but also a powerful secretory organ. During the growth of plants, the root system absorbs nutrients and water from the growth medium to supply the growth of the aboveground part, and on the other hand, it secretes low molecular weight organic compounds (sugars, amino acids, phenolic acids and other secondary metabolites), high molecular weight colloids (polysaccharides, proteins, some phospholipids) and cell shedding and lysate into the growth medium (such as soil, culture solution, etc.), namely root secretions. Root secretions can not only change the physical and chemical properties of the soil, the state of mineral elements in the soil, the distribution of soil biological communities and soil enzyme activity, but also affect the growth and development of surrounding plants.

[0003] The plant rhizosphere is a micro-ecosystem that is affected by the activity of the plant roots and is generally within a few millimeters from the surface of the root axis. It is composed of soil, roots, and microorganisms and has a close interaction with each other. It is the hub that controls the flow of matter, energy, and information exchange in the plant-soil system.

[0004] The study of the effect of plant root-secreted metabolites on rhizosphere soil microorganisms generally uses the soil culture method: that is, metabolites are regularly added to the soil where plants are planted, and then the rhizosphere soil is collected for measurement. However, this method cannot fully focus on the micro-environment of plant roots. The growth of plant roots is complex, and the original metabolites secreted by them cannot be excluded.

[0005] Therefore, it is necessary to provide a fully automatic artificial root system rhizosphere microdomain test device that can simulate the influence of plant root secretion metabolites on rhizosphere microorganisms. Summary of the invention

[0006] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a fully automatic artificial root system multifunctional simulation test device.

[0007] The technical solution adopted by the present invention is as follows: a fully automatic artificial root system multifunctional simulation test device, including a base for supporting and fixing, the side wall of the base is equipped with multiple groups of support platforms for supporting, and the multiple groups of support platforms are evenly arranged on the side wall of the base in a ring shape and at equal intervals, a fixed column is installed at the center of the base, and the fixed column is a cavity structure that passes through from top to bottom, a fixed plate is installed on the upper wall of the fixed column, and the fixed plate is an annular structure, and a telescopic component is movably installed in the fixed column, and the telescopic component is provided with multiple groups, which are telescopic component one, telescopic component two... telescopic component N from bottom to top, the telescopic component one is movably sleeved in the fixed column, and the upper end of the telescopic component one is arranged on the upper end of the fixed plate, the telescopic component two is movably sleeved in the telescopic component one, and the upper end of the telescopic component two is arranged on the upper end of the telescopic component one... the telescopic component N is movably sleeved in the telescopic component N-1, and the telescopic component N The upper end of the base is arranged at the upper end of the telescopic component N-1; a lifting component is also installed at the center of the base, the lifting component is arranged through the telescopic component N, and the upper end of the lifting component is arranged at the upper end of the telescopic component N;

[0008] A sealing bag 1 is installed between the upper wall of the fixed plate and the bottom wall of the telescopic component 1, a sealing bag 2 is installed between the upper wall of the telescopic component 1 and the bottom wall of the telescopic component 2... A sealing bag N is installed between the upper wall of the telescopic component N-1 and the bottom wall of the telescopic component N, and an extrusion bag is installed between the upper wall of the telescopic component N and the bottom wall of the lifting component. The sealing bags 1, 2, N and the extrusion bags are all annular structures. The space inside the sealing bag 1 is a sealing chamber 1, the space inside the sealing bag 2 is a sealing chamber 2... The space inside the sealing bag N is a sealing chamber N, the space inside the extrusion bag is an extrusion chamber, and the sealing chambers 1, 2, N and the extrusion chamber are all sealed chambers.

[0009] Preferably, the side wall of the fixed plate is provided with a plurality of groups of fixing grooves 1 for limiting, and the plurality of groups of fixing grooves 1 are evenly arranged on the outer side wall of the fixed plate in a circular shape with equal intervals, and the fixing grooves 1 are respectively arranged at the upper ends of the support platform, and a ventilation cavity 1 for gas circulation is installed in the fixed plate, and the ventilation cavity 1 is provided with a plurality of groups, and the plurality of groups of ventilation cavity 1 are evenly arranged in the fixed plate in a circular shape with equal intervals, and the ventilation cavity 1 is placed corresponding to the position of the fixing groove 1, and a fixing part is movably installed in any group of the fixing grooves 1, and the fixing part is sealed and connected to the fixing groove 1, and a through cavity 1 is provided in the fixing part, and the upper end of the ventilation cavity 1 is connected to the sealing cavity 1, and the lower end of the ventilation cavity 1 is connected to the through cavity 1, and a control valve is provided in the ventilation cavity 1.

[0010] As a preferred embodiment of the present invention, since the structures of telescopic component 1, telescopic component 2... telescopic component N are the same, only one group is introduced here, the telescopic component 1 includes a telescopic part, the telescopic part is movably arranged in the fixed column, the telescopic part is a cavity structure that passes through from top to bottom, the upper end of the telescopic part extends out of the upper wall of the fixed column, the upper end of the telescopic part is installed with a connecting plate, the connecting plate is arranged at the upper end of the fixed plate, the connecting plate is annular structure, the upper end of the sealing bag 1 is connected to the bottom wall of the connecting plate, the lower end of the sealing bag 1 is connected to the upper wall of the fixed plate, and the telescopic part passes through the sealing bag 1.

[0011] Among them, the side wall of the connecting plate is provided with multiple groups of fixed grooves 2 for limiting, and the multiple groups of fixed grooves 2 are evenly arranged on the outer wall of the connecting plate in a circular shape, and the fixed grooves 2 are arranged at the upper end of the fixed grooves 1. A ventilation cavity 2 for gas circulation is installed in the connecting plate, and the ventilation cavity 2 is provided with multiple groups, and the multiple groups of ventilation cavities 2 are evenly arranged in a circular shape at equal intervals in the connecting plate. The ventilation cavity 2 is placed corresponding to the position of the fixed groove 2, and a connecting component is movably installed in any group of the fixed grooves 2.

[0012] Furthermore, the connecting assembly includes a support plate, a compression plate and a through cavity 2, one end of the support plate is movably arranged on the inner wall of the second fixing groove, and the other end of the support plate extends out of the second fixing groove, the support plate is sealed and connected to the second fixing groove, the support plate is a cavity structure that passes through from left to right, one end of the compression plate is movably clamped in the cavity inside the support plate, the other end of the compression plate extends out of the support plate, the compression plate is sealed and connected to the support plate, a return spring is installed in the support plate, one end of the return spring is connected to the inner wall of the support plate, and the other end is connected to the outer wall of one end of the compression plate, the through cavity 2 is arranged in the compression plate, the upper end of the ventilation cavity 2 is connected to the second sealing cavity, the lower end of the ventilation cavity 2 is connected to the cavity inside the support plate and the through cavity 2 in sequence, a control valve is provided in the ventilation cavity 2, and the ventilation cavity 2 in the telescopic assembly N is connected to the extrusion cavity.

[0013] Among them, a placement groove is provided at the center of the base, and the placement groove is provided in the cavity of the fixed column. The lifting assembly includes a hydraulic rod and a ventilation pipe, the fixed end of the hydraulic rod is provided in the placement groove, and the movable end of the hydraulic rod passes through the cavity of the telescopic assembly N, and the movable end of the hydraulic rod is installed with a lifting plate, and the lifting plate is provided at the upper end of the telescopic assembly N, and the upper end of the extrusion bag is connected to the bottom wall of the lifting plate, and the ventilation pipe is provided in the cavity of the telescopic assembly N. There are multiple groups of ventilation pipes, and the multiple groups of ventilation pipes are evenly arranged in the cavity of the telescopic assembly N in a ring shape at equal intervals. The ventilation pipe is placed corresponding to the position of the fixed groove one, and the number of the support platform, the fixed groove one, the fixed groove two, the ventilation cavity one, the ventilation cavity two and the ventilation pipe are all the same. The inner wall of the lifting plate is provided with a ventilation cavity four, and the upper ends of the ventilation pipes are connected with the ventilation cavity four, and the ventilation cavity four is connected with the extrusion cavity, and a control valve is provided in the ventilation pipe.

[0014] As a preferred embodiment of the present invention, a spring 1 is installed in the sealing bag 1, and the lower end of the spring 1 is connected to the upper wall of the fixed plate, and the upper end of the spring 1 is connected to the bottom wall of the connecting plate in the telescopic component 1; a spring 2 is installed in the sealing bag 2, and the lower end of the spring 2 is connected to the upper wall of the connecting plate in the telescopic component 1, and the upper end of the spring 2 is connected to the bottom wall of the connecting plate in the telescopic component 2… A spring N is installed in the sealing bag N, and the lower end of the spring N is connected to the upper wall of the connecting plate in the telescopic component N-1, and the upper end of the spring N is connected to the bottom wall of the connecting plate in the telescopic component N; an extrusion spring is installed in the extrusion bag, and the lower end of the extrusion spring is connected to the upper wall of the connecting plate in the telescopic component N, and the upper end of the extrusion spring is connected to the bottom wall of the lifting plate.

[0015] Furthermore, an outer baffle plate and an inner baffle plate are installed on the support platform, and the outer baffle plate and the inner baffle plate are placed opposite to each other. The side wall of the outer baffle plate is provided with an elastic bag 1, and the side wall of the inner baffle plate is provided with an elastic bag 2, and the elastic bag 1 and the elastic bag 2 are placed opposite to each other; the lower end of the fixing member and the lower end of the compression plate are both installed with a test box, the test box installed at the lower end of the fixing member and the test box installed at the lower end of the telescopic component... The test box installed at the lower end of the telescopic component N is movably arranged on the upper wall of the support platform from the inside to the outside, and are all arranged between the outer baffle plate and the inner baffle plate, the test box installed at the lower end of the fixing member is arranged close to the inner baffle plate, and the test box installed at the lower end of the telescopic component N is arranged close to the outer baffle plate; the side walls of the test box are respectively installed with elastic bag 3 and elastic bag 4.

[0016] Preferably, a plurality of groups of ventilation cavities three for gas circulation are installed in the base, and the plurality of groups of ventilation cavities three are evenly arranged in the base in a circular shape with equal intervals, and the number of the plurality of ventilation cavities three is the same as the number of ventilation pipes, and the ventilation cavities three respectively penetrate the base and are arranged in the support platform, and the lower ends of the ventilation pipes are respectively connected with the ventilation cavities three, an outer connecting cavity is provided in the outer baffle plate, and the outer connecting cavity is connected with the elastic bag one, and an inner connecting cavity is provided in the inner baffle plate, and the inner connecting cavity is connected with the elastic bag two, and the inner connecting cavity and the outer connecting cavity are respectively connected with the ventilation cavities three in the corresponding support platform.

[0017] Furthermore, a ventilation cavity five is provided in the test box, and the through cavity one and the through cavity two are respectively connected with the ventilation cavity five in the corresponding test box, and the ventilation cavity five is connected with the elastic bladder three and the elastic bladder four; the sealing bladder N and the extrusion bladder are provided with pressure sensors for detecting the pressure of the internal gas, and the lower ends of the elastic bladders one, two, three and four are provided with control valves for discharging the internal gas, and the outer baffle plate, the inner baffle plate and the test box are provided with air holes, and the air holes in the outer baffle plate and the inner baffle plate are arranged on the same straight line as the air holes on the extruded surface of the test box to facilitate the circulation of gas.

[0018] After adopting the above structure, the beneficial effects of the present invention are as follows:

[0019] (1) Multiple groups of test boxes with artificial rhizomes are placed in the test device and can be ventilated with each other. Under the same conditions, the test results of multiple groups can be compared and observed.

[0020] (2) Multiple groups of test boxes with artificial rhizomes are placed in a spiral shape in the test device, and they can be ventilated with each other. Multiple groups of test devices are placed separately, and different test conditions can be adjusted according to needs, such as changing the lighting conditions, changing the test temperature, etc., and then the test results can be compared and observed.

[0021] (3) Multiple groups of test boxes with artificial rhizomes are placed on the same support table, and each group of test boxes is tightly fitted with each other. The air holes in the outer baffle and the inner baffle are arranged on the same straight line as the air holes on the squeezed surface of the test box to facilitate the circulation of gas. At this time, the environments in the multiple groups of test boxes can be integrated with each other, which is convenient for observing the influence of metabolites secreted by the roots of sheet-like artificial plants on the rhizosphere microbial environment.

[0022] (4) Elastic bag three and elastic bag four bulge, and the elastic bags between the inner baffle plate, the test box and the outer baffle plate are in contact with each other, forming a protection for the test box and reducing vibration during movement.

[0023] (5) At the same time, control valves are provided at the lower ends of elastic bag 1, elastic bag 2, elastic bag 3 and elastic bag 4. When a suitable test environment is reached, the control valves are opened at the lower ends of elastic bag 1, elastic bag 2, elastic bag 3 and elastic bag 4 to clean the upper wall of the support platform. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0025] Figure 1 A structural schematic diagram of a fully automatic artificial root system multifunctional simulation test device proposed by the present invention;

[0026] Figure 2 This is a front view of a fully automatic artificial root system multifunctional simulation test device proposed by the present invention;

[0027] Figure 3 for Figure 2 A local enlarged view of point A;

[0028] Figure 4 This is a front and cross-sectional view of a fully automatic artificial root system multifunctional simulation test device proposed by the present invention;

[0029] Figure 5 for Figure 4 A local enlarged view of point B;

[0030] Figure 6 A partial cross-sectional view of a fully automatic artificial root system multifunctional simulation test device proposed by the present invention;

[0031] Figure 7 for Figure 6 A local enlarged view of point C;

[0032] Figure 8 Schematic diagram of the structure of the experiment on the influence of metabolites secreted by artificial plant roots on the rhizosphere microbial environment;

[0033] Fig. 9 Schematic diagram of the structure of the experiment on the influence of artificial plant root secretion metabolites on rhizosphere microbial environment under different environments;

[0034] Fig.10 This is a schematic diagram of the structure when multiple groups of test boxes are transported;

[0035] Fig.11 Schematic diagram of the structure of the experiment on the influence of metabolites secreted by the roots of sheet-shaped artificial plants on the rhizosphere microbial environment;

[0036] Fig.12This is a schematic diagram of the structure in which the fully automatic microinjection pump is connected to the artificial root system in the experiment of how the metabolites secreted by the artificial plant root system affect the rhizosphere microbial environment.

[0037] In the attached drawings: 1, base, 2, support platform, 3, fixed column, 4, fixed plate, 5, telescopic component 1, 6, telescopic component 2, 7, telescopic component 3, 8, lifting component, 9, sealing capsule 1, 10, sealing capsule 2, 11, sealing capsule 3, 12, extrusion capsule, 13, sealing chamber 1, 14, sealing chamber 2, 15, sealing chamber 3, 16, extrusion chamber, 17, fixing groove 1, 18, ventilation chamber 1, 19, fixing part, 20, through chamber 1, 21, telescopic part, 22, connecting plate, 23, fixing groove 2, 24, ventilation chamber 2, 25, connecting component, 26, supporting plate, 27, Compression plate, 28, through cavity two, 29, spring for resetting, 30, placement groove, 31, hydraulic rod, 32, ventilation pipe, 33, lifting plate, 34, ventilation cavity four, 35, spring one, 36, spring two, 37, spring three, 38, spring for extrusion, 39, outer baffle, 40, inner baffle, 41, elastic capsule one, 42, elastic capsule two, 43, test box, 44, elastic capsule three, 45, elastic capsule four, 46, ventilation cavity three, 47, outer connecting cavity, 48, inner connecting cavity, 49, ventilation cavity five, 50, air hole, 51, fully automatic micro-injection pump, 52, artificial root system. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0039] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0040] like Figure 1-Figure 12As shown, a fully automatic artificial root system multifunctional simulation test device comprises a base 1 for supporting and fixing, a plurality of groups of support platforms 2 for supporting are installed on the side wall of the base 1, and the plurality of support platforms 2 are evenly arranged on the side wall of the base 1 in a ring shape and at equal intervals, a fixing column 3 is installed at the center of the base 1, and the fixing column 3 is a cavity structure which passes through from top to bottom, a fixing plate 4 is installed on the upper wall of the fixing column 3, and the fixing plate 4 is a ring structure, a telescopic component is movably installed in the fixing column 3, and the telescopic component is provided with three groups, namely, telescopic component one 5, telescopic component two 6 and telescopic component three 7 from bottom to top, the telescopic component one 5 is movably sleeved in the fixing column 3, and the upper end of the telescopic component one 5 is arranged on the upper end of the fixing plate 4, the telescopic component two 6 is movably sleeved in the telescopic component one 5, and the upper end of the telescopic component two 6 is arranged on the upper end of the telescopic component one 5, the telescopic component three 7 is movably sleeved in the telescopic component two 6, and the telescopic component three 7 The upper end of the telescopic component 2 6 is arranged at the upper end; the base 1 is also equipped with a lifting component 8 at the center, the lifting component 8 is arranged through the telescopic component 3 7, and the upper end of the lifting component 8 is arranged at the upper end of the telescopic component 3 7;

[0041] A sealing bag 19 is installed between the upper wall of the fixed plate 4 and the bottom wall of the telescopic component 15, a sealing bag 210 is installed between the upper wall of the telescopic component 15 and the bottom wall of the telescopic component 26, a sealing bag 311 is installed between the upper wall of the telescopic component 26 and the bottom wall of the telescopic component 37, and an extrusion bag 12 is installed between the upper wall of the telescopic component 37 and the bottom wall of the lifting component 8. The sealing bags 19, 10, 11 and 12 are all annular structures. The space inside the sealing bag 19 is a sealing chamber 13, the space inside the sealing bag 210 is a sealing chamber 214, the space inside the sealing bag 311 is a sealing chamber 315, and the space inside the extrusion bag 12 is an extrusion chamber 16. The sealing chambers 13, 14, 15 and 16 are all sealed chambers.

[0042] It should be noted that the side wall of the fixed plate 4 is provided with a plurality of groups of fixed grooves 17 for limiting, and the plurality of groups of fixed grooves 17 are evenly arranged on the outer wall of the fixed plate 4 in an annular shape and at equal intervals, and the fixed grooves 17 are respectively arranged at the upper end of the support platform 2, and a ventilation cavity 18 for gas circulation is installed in the fixed plate 4, and the ventilation cavity 18 is provided with a plurality of groups, and the plurality of groups of the ventilation cavity 18 are evenly arranged in the fixed plate 4 in an annular shape and at equal intervals, and the ventilation cavity 18 is placed corresponding to the position of the fixed groove 17, and any group of the fixed grooves 17 is movable. A fixing part 19 is dynamically installed, and the fixing part 19 is sealed and connected to the fixing groove 17. A through cavity 20 is arranged in the fixing part 19. The upper end of the ventilation cavity 18 is connected to the sealing cavity 13, and the lower end of the ventilation cavity 18 is connected to the through cavity 20. A control valve is arranged in the ventilation cavity 18 to control whether the gas circulates. After the control valve in the ventilation cavity 18 is opened, when the sealing bag 9 is squeezed, the gas in the sealing cavity 13 enters into the through cavity 20 in the fixing part 19 through the ventilation cavity 18.

[0043] Since the structures of telescopic components 1 5, telescopic components 2 6, and telescopic components 3 7 are all the same, only one group is introduced here. The telescopic component 1 5 includes a telescopic part 21, and the telescopic part 21 is movably arranged in the fixed column 3. The telescopic part 21 is a cavity structure that passes through from top to bottom. The upper end of the telescopic part 21 extends out of the upper wall of the fixed column 3. A connecting plate 22 is installed on the upper end of the telescopic part 21. The connecting plate 22 is arranged at the upper end of the fixed plate 4. The connecting plate 22 is an annular structure. The upper end of the sealing bag 1 9 is connected to the bottom wall of the connecting plate 22, and the lower end of the sealing bag 1 9 is connected to the upper wall of the fixed plate 4. The telescopic part 21 passes through the sealing bag 1 9.

[0044] The side wall of the connecting plate 22 is provided with a plurality of groups of fixing grooves 23 for limiting, and the plurality of groups of fixing grooves 23 are evenly arranged on the outer wall of the connecting plate 22 in a circular shape and at equal intervals, and the fixing grooves 23 are arranged at the upper end of the fixing groove 1 17. A ventilation cavity 24 for gas circulation is installed in the connecting plate 22, and the ventilation cavity 24 is provided with a plurality of groups, and the plurality of groups of ventilation cavities 24 are evenly arranged in a circular shape and at equal intervals in the connecting plate 22, and the ventilation cavity 24 is placed corresponding to the position of the fixing groove 23, and a connecting component 25 is movably installed in any group of the fixing grooves 23.

[0045] The connecting assembly 25 includes a support plate 26, a compression plate 27 and a through cavity 28, one end of the support plate 26 is movably arranged on the inner wall of the second fixing groove 23, and the other end of the support plate 26 extends out of the second fixing groove 23, the support plate 26 is sealed and connected to the second fixing groove 23, the support plate 26 is a cavity structure that passes through the left and right, one end of the compression plate 27 is movably clamped in the inner cavity of the support plate 26, the other end of the compression plate 27 extends out of the support plate 26, the compression plate 27 is sealed and connected to the support plate 26, a return spring 29 is installed in the support plate 26, one end of the return spring 29 is connected to the inner wall of the support plate 26, and the other end The end is connected to the outer side wall of one end of the compression plate 27, the through cavity 28 is arranged in the compression plate 27, the upper end of the ventilation cavity 24 is connected with the sealing cavity 14, and the lower end of the ventilation cavity 24 is connected with the inner cavity of the support plate 26 and the through cavity 28 in sequence. A control valve is provided in the ventilation cavity 24 for controlling whether the gas circulates. After the control valve in the ventilation cavity 24 is opened, when the sealing bag 10 is squeezed, the gas in the sealing cavity 14 enters into the support plate 26 through the ventilation cavity 24, and enters into the through cavity 28 through the support plate 26. The ventilation cavity 24 in the telescopic component 3 7 is connected with the extrusion cavity 16.

[0046] A placement groove 30 is provided at the center of the base 1, and the placement groove 30 is provided in the cavity of the fixed column 3. The lifting component 8 includes a hydraulic rod 31 and a vent pipe 32. The fixed end of the hydraulic rod 31 is provided in the placement groove 30, and the movable end of the hydraulic rod 31 is provided through the cavity of the telescopic component three 7. A lifting plate 33 is installed at the movable end of the hydraulic rod 31, and the lifting plate 33 is provided at the upper end of the telescopic component three 7. The upper end of the extrusion bag 12 is connected to the bottom wall of the lifting plate 33. The vent pipe 32 is provided in the cavity of the telescopic component three 7. A plurality of vent pipes 32 are provided, and the plurality of vent pipes 32 are evenly arranged in a ring shape at equal intervals on the telescopic component. In the cavity of component three 7, the vent pipe 32 is placed corresponding to the position of the fixed groove one 17, the number of the support platform 2, the fixed groove one 17, the fixed groove two 23, the vent cavity one 18, the vent cavity two 24 and the vent pipe 32 are the same, the inner wall of the lifting plate 33 is provided with a vent cavity four 34, the upper ends of the vent pipes 32 are connected with the vent cavity four 34, the vent cavity four 34 is connected with the extrusion cavity 16, and the vent pipes 32 are provided with a control valve for controlling whether the gas circulates. After the control valve in the vent pipe 32 is opened, when the extrusion bag 12 is squeezed, the gas in the extrusion cavity 16 enters the vent pipe 32 through the vent cavity four 34.

[0047] A spring 35 is installed in the sealing capsule 19, and the lower end of the spring 35 is connected to the upper wall of the fixed plate 4, and the upper end of the spring 35 is connected to the bottom wall of the connecting plate 22 in the telescopic component 15. A spring 2 36 is installed in the sealing capsule 2 10, and the lower end of the spring 2 36 is connected to the upper wall of the connecting plate 22 in the telescopic component 1 5, and the upper end of the spring 2 36 is connected to the bottom wall of the connecting plate 22 in the telescopic component 2 6. A spring 37 is installed in the sealing capsule 3 11, and the lower end of the spring 37 is connected to the upper wall of the connecting plate 22 in the telescopic component 2 6, and the upper end of the spring 37 is connected to the bottom wall of the connecting plate 22 in the telescopic component 3 7. An extrusion spring 38 is installed in the extrusion capsule 12, and the lower end of the extrusion spring 38 is connected to the upper wall of the connecting plate 22 in the telescopic component 3 7, and the upper end of the extrusion spring 38 is connected to the bottom wall of the lifting plate 33.

[0048] In this embodiment, initially, the extrusion spring 38, spring 1 35, spring 2 36, and spring 3 37 are all in an uncompressed state, the extrusion bag 12, the sealing bag 1 9, the sealing bag 2 10, and the sealing bag 3 11 are all in a stretched state, and are filled with gas. The number of installations of the fixing member 19 and the connecting assembly 25 is selected as needed, the fixing member 19 is installed in the fixing groove 1 17, the connecting assembly 25 is installed in the fixing groove 2 23, the control valve in the ventilation cavity 1 18 in the fixing groove 1 17 where the fixing member 19 is installed is opened, and the control valves in the other ventilation cavities 1 18 are closed, the control valve in the ventilation cavity 2 24 in the fixing groove 2 23 where the connecting assembly 25 is installed is opened, and the control valves in the other ventilation cavities 2 24 are closed, the control valve in the ventilation pipe 32 placed corresponding to the fixing groove 2 23 where the connecting assembly 25 is installed is opened, and the control valves in the other ventilation pipes 32 are closed, and the hydraulic rod 31 drives The lifting plate 33 moves downward, and the lifting plate 33 drives the extrusion spring 38, and the extrusion spring 38 drives the telescopic component three 7, and the telescopic component three 7 squeezes the spring three 37, and the sealing bag three 11 is compressed, and the gas in the sealing chamber three 15 enters the ventilation chamber two 24 in the telescopic component two 6, and the spring three 37 compresses the telescopic component two 6, and the telescopic component two 6 squeezes the spring two 36, and the sealing bag two 10 is compressed, and the gas in the sealing chamber two 14 enters the ventilation chamber two 24 in the telescopic component one 5, and the telescopic component one 5 squeezes the spring one 35, and the sealing bag one 9 is compressed, and the gas in the sealing chamber one 13 enters the ventilation chamber one 18 in the fixing plate 4, and the gas in the ventilation chamber one 18 enters the through chamber one 20 in the fixing part 19, and the gas in the ventilation chamber two 24 enters the support plate 26 and enters the through chamber two 28 through the support plate 26.

[0049] An outer baffle plate 39 and an inner baffle plate 40 are installed on the support platform 2, and the outer baffle plate 39 and the inner baffle plate 40 are placed opposite to each other. The side wall of the outer baffle plate 39 is provided with an elastic capsule 1 41, and the side wall of the inner baffle plate 40 is provided with an elastic capsule 2 42, and the elastic capsule 1 41 and the elastic capsule 2 42 are placed opposite to each other; a test box 43 is installed at the lower end of the fixing member 19 and the lower end of the compression plate 27, and the test box 43 installed at the lower end of the fixing member 19 and the test box 43 installed at the lower end of the telescopic component 15... The test box 43 installed at the lower end of the telescopic component 37 are movably arranged on the upper wall of the support platform 2 from the inside to the outside, and are all arranged between the outer baffle plate 39 and the inner baffle plate 40, the test box 43 installed at the lower end of the fixing member 19 is arranged close to the inner baffle plate 40, and the test box 43 installed at the lower end of the telescopic component 37 is arranged close to the outer baffle plate 39;

[0050] It should be noted that elastic bladder three 44 and elastic bladder four 45 are respectively installed on the side walls of the test box 43, and a ventilation cavity five 49 is provided in the test box 43. The through cavity one 20 and the through cavity two 28 are respectively connected with the ventilation cavity five 49 in the corresponding test box 43, and the ventilation cavity five 49 is connected with the elastic bladder three 44 and the elastic bladder four 45.

[0051] A plurality of ventilation cavities 3 46 for gas circulation are installed in the base 1. The plurality of ventilation cavities 3 46 are evenly arranged in the base 1 in a circular shape at equal intervals. The number of the plurality of ventilation cavities 3 46 is the same as the number of ventilation tubes 32. The ventilation cavities 3 46 respectively penetrate the base 1 and are arranged in the support platform 2. The lower ends of the ventilation tubes 32 are respectively connected with the ventilation cavities 3 46. An outer connecting cavity 47 is provided in the outer baffle plate 39. The outer connecting cavity 47 is connected with the elastic capsule 1 41. An inner connecting cavity 48 is provided in the inner baffle plate 40. The inner connecting cavity 48 is connected with the elastic capsule 2 42. The inner connecting cavity 48 and the outer connecting cavity 47 are respectively connected with the ventilation cavities 3 46 in the corresponding support platform 2. The gas in the extrusion cavity 16 enters the inner connecting cavity 48 and the outer connecting cavity 47 through the ventilation cavity 44 34, the ventilation tube 32 and the ventilation cavity 3 46 in turn to blow the elastic capsule 2 42 and the elastic capsule 1 41.

[0052] In this embodiment, pressure sensors are provided in the sealing bag three 11 and the extrusion bag 12 for detecting the pressure of the internal gas. Control valves are provided at the lower ends of the elastic bag one 41, the elastic bag two 42, the elastic bag three 44 and the elastic bag four 45 to facilitate the discharge of the internal gas. Air holes 50 are provided on the outer baffle plate 39, the inner baffle plate 40 and the test box 43, and the air holes 50 in the outer baffle plate 39 and the inner baffle plate 40 are arranged on the same straight line as the air holes 50 on the extruded surface of the test box 43 to facilitate the circulation of gas. The pressure sensor is electrically connected to the hydraulic rod 31 through a controller.

[0053] The specific usage is as follows:

[0054] The telescopic components are provided with three groups, namely, telescopic component one 5, telescopic component two 6 and telescopic component three 7. The present invention provides a fully automatic artificial root system simulation test device for studying the influence of plant root secretion on the rhizosphere micro-domain environment, such as Fig.12 The fully automatic micro-injection pump 51 is connected to one end of the artificial root system 52 , and the other end of the artificial root system 52 is buried in the test box. The fully automatic micro-injection pump 51 is used to add a specific root secretion solution into the artificial root system 52 .

[0055] 1. The operation of the experiment on the influence of multiple groups of artificial plant root secretion metabolites on rhizosphere microbial environment is as follows:

[0056] like Figure 1 , Figure 8 and Fig. 9 Three simulation test methods are shown:

[0057] Figure 1 and Figure 8 In the experiment, multiple test boxes 43 with artificial rhizomes are placed in the test device and can be ventilated with each other. Under the same conditions, Figure 1 The results of multiple groups of tests can be compared and observed. Figure 8 It is also possible to compare and observe multiple groups of test results.

[0058] Fig. 9 In the present invention, multiple test boxes 43 with artificial rhizomes are placed in a spiral shape in the test device and can be ventilated with each other. Multiple test devices are placed separately, and different test conditions can be adjusted according to needs, such as changing the lighting conditions, changing the test temperature, etc., and then the test results can be compared and observed.

[0059] 2. The operation of the experiment to simulate the influence of metabolites secreted by the roots of artificial plants on the rhizosphere microbial environment is as follows:

[0060] like Fig.11 The simulation test method shown is:

[0061] Place multiple groups of test boxes 43 with artificial rhizomes on the same support platform 2, and each group of test boxes 43 fits closely to each other, and the air holes 50 in the outer baffle 39 and the inner baffle 40 are arranged on the same straight line as the air holes 50 on the squeezed surface of the test box 43, so as to facilitate the circulation of gas. At this time, the environments in the multiple groups of test boxes 43 can be integrated with each other, so as to facilitate the observation of the influence of metabolites secreted by the roots of the sheet-like artificial plants on the rhizosphere microbial environment;

[0062] The operation process of each set of test boxes 43 being closely attached to each other is as follows:

[0063] The control valve in the ventilation chamber 18 and the control valve in the ventilation chamber 24 are both in a closed state, the extrusion spring 38, spring 1 35, spring 2 36, and spring 37 are all in an uncompressed state, the extrusion bag 12, the sealing bag 1 9, the sealing bag 2 10, and the sealing bag 3 11 are all in a stretched state, and are filled with gas. The number of installation of the fixing member 19 and the connecting component 25 is selected according to the needs, the fixing member 19 is installed in the fixing groove 1 17, and the connecting component 25 is installed in the fixing groove 2 23. The control valve in the ventilation pipe 32 placed corresponding to the fixing groove 23 where the connecting component 25 is installed is opened, and the control valves in the other ventilation pipes 32 are closed, and the hydraulic rod 31 drives the lifting plate 33 to move downward, and the lifting plate 33 drives the extrusion spring 38, and the extrusion spring 38 is compressed, and the extrusion bag 12 is squeezed. , the gas in the extrusion chamber 16 enters the inner connecting chamber 48 and the outer connecting chamber 47 through the ventilation chamber 434, the ventilation pipe 32, and the ventilation chamber 3 46 in turn, blowing the elastic capsule 2 42 and the elastic capsule 1 41. The elastic capsule 2 42 protects the closest test box 43. The elastic member 1 swells and pushes the closest test box 43. The test box 43 moves toward the direction close to the inner baffle 40, driving the compression plate 27 to insert into the support plate 26, and the reset spring 29 is compressed until it fits the next group of test boxes 43. The two groups of test boxes 43 move together until the four groups of test boxes 43 are tightly fitted to each other. As the hydraulic rod 31 continues to move downward, the pressure sensor values ​​in the extrusion bag 12 and the sealing bag 3 11 increase, and the signal is transmitted to the controller, and the controller stops the operation of the hydraulic rod 31.

[0064] 3. When it is necessary to simulate the effects of multiple groups of artificial plant root secretion metabolites on the rhizosphere microbial environment and simulate the effects of sheet-shaped artificial plant root secretion metabolites on the rhizosphere microbial environment for comparative tests:

[0065] First, according to method 2, the test box 43 for the influence of the root secretion metabolites of the sheet-shaped artificial plant on the rhizosphere microbial environment is placed on the same group of support platforms 2, and then according to the needs Figure 8 or Fig. 9 The remaining test boxes 43 can be installed in the same manner.

[0066] When the test box 43 needs to be moved to a suitable test environment, in order to move multiple groups of test boxes 43 at the same time and reduce the shaking of the test boxes 43 during the movement, first install Figure 1 Assemble the test box 43 and then take the following steps:

[0067] Assume that initially, the extrusion spring 38, spring 1 35, spring 2 36, and spring 3 37 are all in an uncompressed state, and the extrusion bag 12, the sealing bag 1 9, the sealing bag 2 10, and the sealing bag 3 11 are all in a stretched state. The number of fixing members 19 and the connecting assembly 25 to be installed is selected according to the needs. The fixing member 19 is installed in the fixing groove 1 17, and the connecting assembly 25 is installed in the fixing groove 2 23. The control valve in the ventilation cavity 1 18 in the fixing groove 1 17 where the fixing member 19 is installed is opened, and the control valves in the other ventilation cavities 1 18 are closed. The control valve in the ventilation cavity 2 24 in the fixing groove 2 23 where the connecting assembly 25 is installed is opened, and the control valves in the other ventilation cavities 2 24 are closed. The control valve in the vent pipe 32 correspondingly placed in the fixing groove 23 of the connecting component 25 is opened, and the control valves in the other vent pipes 32 are closed. The hydraulic rod 31 drives the lifting plate 33 to move downward, and the lifting plate 33 drives the extrusion spring 38. The extrusion spring 38 is compressed, and the extrusion bag 12 is squeezed. The gas in the extrusion chamber 16 enters the inner connecting chamber 48 and the outer connecting chamber 47 through the ventilation chamber 4 34, the vent pipe 32, and the ventilation chamber 3 46 in turn, and the elastic bag 2 42 and the elastic bag 1 41 are blown. The elastic bag 2 42 protects the closest test box 43. The elastic member 1 swells and pushes the closest test box 43. At the same time, the gas in the extrusion bag 12 also passes through the telescopic component The ventilation cavity 24 in the three 7 enters into the support plate 26, and enters into the through cavity 28 through the support plate 26. The extrusion spring 38 drives the telescopic component three 7. The telescopic component three 7 squeezes the spring three 37. The sealing capsule three 11 is compressed. The gas in the sealing cavity three 15 enters into the support plate 26 through the ventilation cavity 24 in the telescopic component two 6, and enters into the through cavity two 28 through the support plate 26. The spring three 37 compresses the telescopic component two 6. The sealing capsule two 10 is compressed. The gas in the sealing cavity two 14 enters into the support plate 26 through the ventilation cavity two 24 in the telescopic component one 5, and enters into the through cavity two 28 through the support plate 26. The telescopic component one 5 The spring 35 is squeezed, the sealing capsule 9 is compressed, the gas in the sealing chamber 13 enters the ventilation chamber 18 in the fixing plate 4, the gas in the ventilation chamber 18 enters the through chamber 20 in the fixing member 19, the through chamber 20 and the through chamber 28 are respectively connected with the ventilation chamber 5 49 in the corresponding test box 43, the squeezed gas is connected with the elastic capsule 3 44 and the elastic capsule 4 45 through the ventilation chamber 5 49, the elastic capsule 3 44 and the elastic capsule 4 45 swell, and the elastic capsules between the inner baffle plate 40, the test box 43 and the outer baffle plate 39 are in contact with each other, forming a protection for the test box 43, reducing vibration during movement, and when reaching a suitable test environment, in accordance with Figure 1 , Figure 8 as well as Fig. 9 Install it as shown.

[0068] At the same time, control valves are provided at the lower ends of elastic capsule 1 41 , elastic capsule 2 42 , elastic capsule 3 44 and elastic capsule 4 45 . When a suitable test environment is reached, the control valves are opened at the lower ends of elastic capsule 1 41 , elastic capsule 2 42 , elastic capsule 3 44 and elastic capsule 4 45 to clean the upper wall of support platform 2 .

[0069] Although the embodiments of the present invention have been shown and described, it is understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents. In short, if those skilled in the art are inspired by them and design structural modes and embodiments similar to the technical solution without creativity without departing from the purpose of the present invention, they shall all fall within the scope of protection of the present invention.

Claims

1. A fully automatic multifunctional artificial root simulation test device, comprising a base for supporting and fixing, the side wall of the base is equipped with multiple groups of support platforms for supporting, the multiple groups of support platforms are evenly arranged on the side wall of the base in a ring shape, a fixed column is installed in the center of the base, a fixed plate is installed on the upper wall of the fixed column, a telescopic component is movably installed in the fixed column, and the telescopic component is provided with multiple groups, which are telescopic component 1, telescopic component 2... telescopic component N from bottom to top, the telescopic component 1 is movably sleeved in the fixed column, and the upper end of the telescopic component 1 is arranged on the upper end of the fixed plate, the telescopic component 2 is movably sleeved in the telescopic component 1, and the upper end of the telescopic component 2 is arranged on the upper end of the telescopic component 1... the telescopic component N is movably sleeved in the telescopic component N-1, and the upper end of the telescopic component N is arranged on the upper end of the telescopic component N-1; a lifting component is also installed in the center of the base, the lifting component is arranged through the telescopic component N, and the upper end of the lifting component is arranged on the upper end of the telescopic component N; A sealing capsule 1 is installed between the upper wall of the fixed plate and the bottom wall of the telescopic component 1, a sealing capsule 2 is installed between the upper wall of the telescopic component 1 and the bottom wall of the telescopic component 2... a sealing capsule N is installed between the upper wall of the telescopic component N-1 and the bottom wall of the telescopic component N, an extrusion capsule is installed between the upper wall of the telescopic component N and the bottom wall of the lifting component, the sealing capsule 1, the sealing capsule 2, the sealing capsule N and the extrusion capsule are all annular structures, the space inside the sealing capsule 1 is the sealing cavity 1, the space inside the sealing capsule 2 is the sealing cavity 2... the space inside the sealing capsule N is the sealing cavity N, the space inside the extrusion capsule is the extrusion cavity, the sealing cavity 1, the sealing cavity 2, the sealing cavity N and the extrusion cavity are all sealed chambers; The side wall of the fixed plate is provided with a plurality of groups of fixing grooves 1 for limiting, and the plurality of groups of fixing grooves 1 are evenly arranged on the outer side wall of the fixed plate 1 in a circular shape with equal intervals, and the fixing grooves 1 are respectively arranged at the upper ends of the support platform, and a ventilation cavity 1 for gas circulation is installed in the fixed plate, and the ventilation cavity 1 is provided with a plurality of groups, and the plurality of groups of ventilation cavity 1 are evenly arranged in the fixed plate in a circular shape with equal intervals, and the ventilation cavity 1 is placed corresponding to the position of the fixing groove 1, and a fixing piece is movably installed in any group of the fixing grooves 1, and a through cavity 1 is provided in the fixing piece, and the upper end of the ventilation cavity 1 is connected with the sealing cavity 1, and the lower end of the ventilation cavity 1 is connected with the through cavity 1, and a control valve is provided in the ventilation cavity 1; The telescopic component 1 includes a telescopic member, which is movably arranged in the fixed column, and the telescopic member is a cavity structure that passes through from top to bottom. The upper end of the telescopic member extends out of the upper wall of the fixed column, and a connecting plate is installed on the upper end of the telescopic member, and the connecting plate is arranged on the upper end of the fixed plate. The upper end of the sealing bag 1 is connected to the bottom wall of the connecting plate, and the lower end of the sealing bag 1 is connected to the upper wall of the fixed plate. The telescopic member passes through the sealing bag 1; The side wall of the connecting plate is provided with a plurality of groups of fixing grooves 2 for limiting, and the plurality of groups of fixing grooves 2 are evenly arranged on the outer side wall of the connecting plate in an annular shape and at equal intervals, and the fixing grooves 2 are arranged at the upper end of the fixing grooves 1. A ventilation cavity 2 for gas circulation is installed in the connecting plate, and the ventilation cavity 2 is provided with a plurality of groups, and the plurality of groups of ventilation cavities 2 are evenly arranged in the connecting plate in an annular shape and at equal intervals, and the ventilation cavity 2 is placed corresponding to the position of the fixing grooves 2, and a connecting component is movably installed in any group of the fixing grooves 2; The connecting assembly includes a support plate, a compression plate and a through cavity II, one end of the support plate is movably arranged on the inner side wall of the second fixing groove, and the other end of the support plate is extended out of the second fixing groove, the support plate is a cavity structure that is through-going from left to right, one end of the compression plate is movably connected in the cavity of the support plate, and the other end of the compression plate is extended out of the support plate, a return spring is installed in the support plate, one end of the return spring is connected to the inner side wall of the support plate, and the other end is connected to the outer side wall of one end of the compression plate, the through cavity II is arranged in the compression plate, the upper end of the ventilation cavity II is connected to the sealing cavity II, the lower end of the ventilation cavity II is connected to the cavity of the support plate and the through cavity II in turn, a control valve is arranged in the ventilation cavity II, and the ventilation cavity II in the telescopic assembly N is connected to the extrusion cavity; A placement slot is provided at the center of the base, and the placement slot is provided in the cavity of the fixed column. The lifting assembly includes a hydraulic rod and a vent pipe. The fixed end of the hydraulic rod is provided in the placement slot, and the movable end of the hydraulic rod is provided through the cavity of the telescopic assembly N. A lifting plate is installed at the movable end of the hydraulic rod, and the lifting plate is provided at the upper end of the telescopic assembly N. The upper end of the extrusion bag is connected to the bottom wall of the lifting plate, and the vent pipe is provided in the cavity of the telescopic assembly N. There are multiple groups of vent pipes, and the multiple groups of vent pipes are evenly arranged in the cavity of the telescopic assembly N in a ring shape at equal intervals. The vent pipe is placed corresponding to the position of the fixed slot one, and a ventilation cavity four is provided on the inner wall of the lifting plate. The upper ends of the vent pipes are connected with the ventilation cavity four, and the ventilation cavity four is connected with the extrusion cavity, and a control valve is provided in the vent pipe; The sealing capsule 1 is provided with a spring 1, the lower end of which is connected to the upper wall of the fixed plate, and the upper end of which is connected to the bottom wall of the connecting plate in the telescopic component 1; the sealing capsule 2 is provided with a spring 2, the lower end of which is connected to the upper wall of the connecting plate in the telescopic component 1, and the upper end of which is connected to the bottom wall of the connecting plate in the telescopic component 2... The sealing capsule N is provided with a spring N, the lower end of which is connected to the upper wall of the connecting plate in the telescopic component N-1, and the upper end of which is connected to the bottom wall of the connecting plate in the telescopic component N; the extrusion capsule is provided with an extrusion spring, the lower end of which is connected to the upper wall of the connecting plate in the telescopic component N, and the upper end of which is connected to the bottom wall of the lifting plate; An outer baffle and an inner baffle are installed on the support platform, and the outer baffle and the inner baffle are placed opposite to each other. The side wall of the outer baffle is provided with an elastic bag 1, and the side wall of the inner baffle is provided with an elastic bag 2, and the elastic bag 1 and the elastic bag 2 are placed opposite to each other; the lower end of the fixing member and the lower end of the compression plate are both installed with test boxes, the test box installed at the lower end of the fixing member and the test box installed at the lower end of the telescopic component... The test box installed at the lower end of the telescopic component N is movably arranged on the wall of the support platform from the inside to the outside, and is arranged between the outer baffle and the inner baffle, the test box installed at the lower end of the fixing member is arranged close to the inner baffle, and the test box installed at the lower end of the telescopic component N is arranged close to the outer baffle; the side walls of the test box are respectively installed with elastic bags 3 and 4; A plurality of groups of ventilation cavities three for gas circulation are installed in the base, and the plurality of groups of ventilation cavities three are evenly arranged in the base in a circular shape with equal intervals, and the number of the plurality of ventilation cavities three is the same as the number of ventilation pipes, and the ventilation cavities three are respectively penetrated through the base and arranged in the support platform, and the lower ends of the ventilation pipes are respectively connected with the ventilation cavities three, and an outer connecting cavity is provided in the outer baffle plate, and the outer connecting cavity is connected with the elastic bladder one, and an inner connecting cavity is provided in the inner baffle plate, and the inner connecting cavity is connected with the elastic bladder two, and the inner connecting cavity and the outer connecting cavity are respectively connected with the ventilation cavities three in the corresponding support platform; A ventilation cavity five is provided in the test box, and the through cavity one and the through cavity two are respectively connected with the ventilation cavity five in the corresponding test box, and the ventilation cavity five is connected with the elastic bladder three and the elastic bladder four; a pressure sensor is provided in the sealing bladder N and the extrusion bladder, and a control valve is provided at the lower end of the elastic bladder one, the elastic bladder two, the elastic bladder three and the elastic bladder four, and air holes are provided on the outer baffle plate, the inner baffle plate and the test box, and the air holes in the outer baffle plate and the inner baffle plate are arranged on the same straight line as the air holes on the extruded surface of the test box.

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